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All Journal Jurnal Penelitian Pertanian Terapan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Inovasi Journal of Horticulture Production Technology
Lisa Erfa
Politeknik Negeri Lampung
Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Respon Pertumbuhan Seedling Anggrek Dendrobium Gradita 31 Pada Beberapa Kombinasi Media Ms dan Ekstrak Taoge Dwi Dhita Apriyani; Yeni Yeni; Wika Anrya Darma; Lisa Erfa
Journal of Horticulture Production Technology Vol 4 No 1 (2026)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jhpt.v4i1.4635

Abstract

Problems in orchid development are still quite limited due to the lack of availability of uniform seeds and the slow growth process of orchids, resulting in limited availability of orchid plant seeds. Subculture is the transfer of plants by in vitro culture from old media to new media with a specific purpose. One type of organic PGR used is bean sprout extract which contains auxin and cytokinin for the growth of plant seedlings. The objectives of this study are, (1) To obtain the best concentration of MS media at the growth stage of Dendrobium "Gradita" orchids, (2) To obtain the best concentration of bean sprout extract at the growth stage of Dendrobium "Gradita 31" orchids, (3) To determine the interaction between MS media and bean sprout extract at the growth stage of Dendrobium "Gradita 31" orchids, and (4) To obtain the best combination between MS media and bean sprout extract at the growth stage of Dendrobium "Gradita 31" orchids. The experiment will be conducted using a factorial Randomized Block Design (RAK). The first factor is the concentration of MS media, namely MS media ½ MS and full MS. The second factor is the concentration of bean sprout extract, namely 0 g.l-1, 100 g.l-1, 200 g.l-1, and 300 g.l-1. The research data were processed using analysis of variance, to see the differences between treatments, then continued with the Least Significant Difference (LSD) test at a level of 5%. The conclusions from the results of this study are (1) The best concentration of MS media use is at the level of concentration of bean sprout extract addition used in increasing seedling height, number of leaves, number of roots, root length, and seedling weight in the use of MS media. (2) The best concentration of bean sprout extract use is at the level of MS media use used in increasing seedling height, number of leaves, number of roots, root length, and seedling weight in the concentration of bean sprout extract 0 g.l-1 and 100 g.l-1. (3) There is an interaction between the combination of MS media and bean sprout extract on the increase in seedling height and seedling weight, and (4) There is the best combination of bean sprout extract, namely in ½ MS media with the addition of 0 g.1-1 bean sprout extract which results in an increase in seedling height, number of leaves, leaf length, and plant weight.
Pertumbuhan Planlet Dendrobium ‘Linda Febriana’ × ‘Nindii’ secara In Vitro pada Berbagai Konsentrasi BAP dan NAA dalam Media Subkultur Intan Triani; Lisa Erfa; Sekar Utami Putri; Dila Febria
Journal of Horticulture Production Technology Vol 4 No 1 (2026)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jhpt.v4i1.4639

Abstract

Orchid propagation through in vitro techniques takes 9-12 months. Culture media plays a very important role in supporting the growth and development of explants. In addition to macro and micro nutrients, sugar, and vitamins, the use of plant growth regulators (PGRs) is needed to accelerate the growth of explants. The objectives of this study were: (a) to obtain the best BAP concentration for the growth of orchid seedlings, (b) to obtain the best NAA concentration for the growth of orchid seedlings, (c) to determine whether there is an interaction between the administration of BAP and NAA concentrations on the growth of orchid seedlings, and (d) to obtain the best combination of BAP and NAA concentrations for the growth of Dendrobium orchids. This study used a 2-factor Randomized Block Design. The first factor was BAP with levels of 1 mg.l-1 (B1), 2 mg.l-1 (B2), and 3 mg.l-1 (B3). While the second factor was NAA with levels of 1 mg.l-1 (N1), 2 mg.l-1 (N2), and 3 mg.l-1 (N3). Observation parameters include increase in seedling height, increase in number of leaves, increase in number of roots, shoot height, and number of shoots. Observation data were analyzed for variance and continued with BNJ test at 5% level. The results of this study indicate that: (a) the best BAP concentration on increasing seedling height, increasing number of leaves, and increasing number of roots is BAP 1 mg.l-1, while on the variable shoot height and number of shoots is BAP concentration 2 mg.l-1, (b) The best NAA concentration is 2 mg.l-1 on increasing seedling height, increasing number of leaves, and increasing number of roots, (c) there is an interaction between BAP and NAA concentrations on increasing seedling height, increasing number of leaves, and increasing number of roots, (d) the best combination of BAP and NAA concentrations is BAP 1 mg.l-1 with NAA 2 mg.l-1 on increasing seedling height, increasing number of leaves, and increasing number of roots.
Co-Authors Amalia Hartias Anggraini Desi Maulida Dulbari, Dulbari Dwi Dhita Apriyani Evi Yunita Sari Febria, Dila Ferziana Ferziana I Gde Darmaputra Intan Triani Marveldani Prajaka, Nanang Wahyu Pretty Aulia Dewi Fortuna Putri, Sekar Utami Rahhutami, Ratih Rahmadyah Hamiranti Raida Kartina Rizka Novi Sesanti Rizka novi sesanti Shendi Elendrya Sismanto Sismanto Wika Anrya Darma, Wika Anrya Yeni Yeni Yuriansyah Yuriansyah Yuriansyah Yuriansyah Yuriansyah, Yuriansyah Zainal Mutaqin