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Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis ATCC 10556) Nurdin, Denny; Dharsono, Hendra Dian Adhita; Fatriadi, Fajar; Sahara, Elvi; Adang, Rahmi Alma Farah; Gondowidjojo, Yolanda Dwiutami; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.37043

Introduction: Streptococcus sanguinis is a facultative anaerobic Gram-positive bacteria known as a pioneer that plays a role in creating the biofilm in the oral cavity. Strawberry fruit (Fragaria x ananassa) is an edible fruit widely used for the study as their active compound synergy to improve health. This study aims to analyse the antibacterial activity of strawberry fruit against S. sanguinis (ATCC 10556). Methods: An explorative study was conducted with high concentration methanol extract from strawberry fruit. Antibacterial activity was tested on the methanol extract; afterwards, the extract was fractionated and divided into three fractions: water, ethyl acetate, and hexane. Zone of inhibition was used to assess the most effective fraction among those three, then continued by testing for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Inhibitory zone of the ethyl acetate fraction from strawberry fruit 1%, 2%, 3% ,4% and 5% sequentially were 7.3 mm,10.2 mm, 12.3 mm, 16.3 mm and 16.1 mm. Ethyl acetate fraction of 4% was the most effective to create the zone of inhibition with a size of 16.3 mm compared to the others. The Minimum Inhibitory Concentration (MIC) value was 0.25%. It was obtained by diluting a 4% ethyl acetate fraction on a microplate. The Minimum Bactericidal Concentration (MBC) was 2%. Conclusion: Ethyl acetate fraction was an effective fraction from strawberry fruit and had antibacterial activity against S. sanguinis with the inhibitory zone in the concentration of 4%, MIC of 0.25%, and MBC of 2%.

Perawatan Saluran Akar dengan teknik Step Back pada Kasus Pulpitis Irreversible dengan Jaringan Apikal Normal Gigi Insisif Mandibula Pratiwi, Amatul Firdaustia; Fatriadi, Fajar
Journal of Syiah Kuala Dentistry Society Vol 9, No 2 (2024): December 2024
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v9i2.39035

Introduction: Caries is a bacterial infection of the teeth that can attack the enamel, dentin, and pulp. Caries extending to the pulp can result in irreversible pulpitis, where root canal treatment is indicated so the tooth can return functionally and aesthetically. Case report: A 50-year-old woman came with chief complaints of cavities in her lower front teeth. From subjective, objective, and radiographic examination, tooth 31 was diagnosed with irreversible pulpitis with normal apical tissue, and a root canal treatment was planned. In the next visit, the root canal treatment was carried out using the step-back technique starting from IAF K-File #10 and MAF K-File #25 with a working length of 22.5 mm. The final K-File used was K-File #40, with a working length of 19.5 mm. Root canal treatment on tooth 31 was carried out in 10 visits. After radiographic evaluation subjective and objective examination, the root canal treatment was completed, and a post-crown restoration was planned as a follow-up. Conclusion: Root canal treatment in 10 visits on tooth 31, which was diagnosed with irreversible pulpitis with normal apical tissue, carried out using the step-back technique in this case, showed clinical and radiological success.

AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

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