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The most common and predicted diagnosis or conditions of oral mucosal lesions among elementary school children Wahyuni, Indah Suasani; Putri, Fidya Meditia; Fatriadi, Fajar; Nur'aeny, Nanan; Hidayat, Wahyu
Padjadjaran Journal of Dentistry Vol 33, No 2 (2021): July 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no2.15977

Introduction: Oral mucosal health is part of general health that also requires attention. Research on oral mucosal in children has not widely conducted, especially in the Jatinangor sub-district as one of the educational centres in West Java. This study was aimed to determine the most common and predicted the diagnosis or conditions of oral mucosal lesions among elementary school children. Methods: This research was a cross-sectional study. The purposive sampling method was used, in which only the fifth-grade students from the elementary school cluster near the centre of the Jatinangor sub-district were involved. The inclusion criteria were healthy condition, cooperative, able to communicate with the examiner, and obtained permission from the parent/guardian. The oral mucosal lesions were examined using disposable dental tools and a white light headlamp. Oral mucosal lesions found on examination defined as oral diseases and normal variation of oral mucosal. Results: A total of 226 students, consisting of 115 males and 111 females, were included in this study. The oral mucosal lesions found were described as follows: 100 cases of pseudomembranous plaque, 106 cases of macula, 56 cases of ulceration, 45 cases of cheek biting, and another fewer lesions. The lesions' diagnosis was coated tongue for the pseudomembranous plaque, physiologic pigmentation for the macula, aphthous stomatitis/traumatic ulcer/angular cheilitis for ulcers, linea alba for cheek biting, and others. Conclusion: The most commonly found oral mucosal lesions were macula which is diagnosed as macular hyperpigmentation, followed by pseudomembranous plaque which is diagnosed as coated tongue.

Antibacterial potential of strawberries and basil extracts combination against Streptococcus sanguinis (ATCC 10556) Dharsono, Hendra Dian Adhita; Nurdin, Denny; Fatriadi, Fajar; Gondowidjojo, Yolanda Dwiutami; Yunita, Ellizabeth; Apriyanti, Eti
Padjadjaran Journal of Dentistry Vol 33, No 3 (2021): November
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no3.35894

Introduction: Streptococcus sanguinis is a commensal microorganism as well as a pioneer colony in forming dental plaque. Oral biofilm formation can be prevented by a mechanical cleaning procedure followed by the use of mouthwash. The current gold standard for mouthwash is chlorhexidine. Nevertheless, it has side effects that are not recommended for long-term use. Previous studies had proven that herbal-based mouthwashes such as basil leaves (Ocimum basilicum) and strawberry fruit (Fragaria x ananassa) have been shown to have antibacterial properties. The effectivity of antibacterial activity phenomenon in combined extracts has been reported in other studies. This research aims to observe the antibacterial potential of the F. x ananassa and O. basilicum extract combinations against S. sanguinis (ATCC 10556). Methods: The sample of this study was a combination of F. x ananassa and O. basilicum extract, which initially screened for their antibacterial activities. Antibacterial activities of F. x ananassa and O. basilicum extracts against S. sanguinis were observed using Kirby Bauer method, while Minimum Inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by serial microdilution method. The 2% concentration from each extract was combined in 1:1, 1:2, and 2:1 volume ratio variations then tested for inhibitory zones, MIC, and MBC. Results: F. x ananassa extract had 0.125% and 0.25% for MIC and MBC respectively, while O. basilicum extract showed the value of MIC and MBC as 0.031% and 0.063% against S. sanguinis (ATCC 10556). The extract combinations in 1:1, 1:2, and 2:1 volume ratio variations showed 0.016% for MIC and 0.031% for MBC. Conclusions: It was concluded that combining extracts of 2 % F. x ananassa and 2% O. basilicum in various ratios were observably to have the antibacterial potential against S. sanguinis (ATCC 10556).

Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis ATCC 10556) Nurdin, Denny; Dharsono, Hendra Dian Adhita; Fatriadi, Fajar; Sahara, Elvi; Adang, Rahmi Alma Farah; Gondowidjojo, Yolanda Dwiutami; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.37043

Introduction: Streptococcus sanguinis is a facultative anaerobic Gram-positive bacteria known as a pioneer that plays a role in creating the biofilm in the oral cavity. Strawberry fruit (Fragaria x ananassa) is an edible fruit widely used for the study as their active compound synergy to improve health. This study aims to analyse the antibacterial activity of strawberry fruit against S. sanguinis (ATCC 10556). Methods: An explorative study was conducted with high concentration methanol extract from strawberry fruit. Antibacterial activity was tested on the methanol extract; afterwards, the extract was fractionated and divided into three fractions: water, ethyl acetate, and hexane. Zone of inhibition was used to assess the most effective fraction among those three, then continued by testing for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Inhibitory zone of the ethyl acetate fraction from strawberry fruit 1%, 2%, 3% ,4% and 5% sequentially were 7.3 mm,10.2 mm, 12.3 mm, 16.3 mm and 16.1 mm. Ethyl acetate fraction of 4% was the most effective to create the zone of inhibition with a size of 16.3 mm compared to the others. The Minimum Inhibitory Concentration (MIC) value was 0.25%. It was obtained by diluting a 4% ethyl acetate fraction on a microplate. The Minimum Bactericidal Concentration (MBC) was 2%. Conclusion: Ethyl acetate fraction was an effective fraction from strawberry fruit and had antibacterial activity against S. sanguinis with the inhibitory zone in the concentration of 4%, MIC of 0.25%, and MBC of 2%.

Perawatan Saluran Akar dengan teknik Step Back pada Kasus Pulpitis Irreversible dengan Jaringan Apikal Normal Gigi Insisif Mandibula Pratiwi, Amatul Firdaustia; Fatriadi, Fajar
Journal of Syiah Kuala Dentistry Society Vol 9, No 2 (2024): December 2024
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jds.v9i2.39035

Introduction: Caries is a bacterial infection of the teeth that can attack the enamel, dentin, and pulp. Caries extending to the pulp can result in irreversible pulpitis, where root canal treatment is indicated so the tooth can return functionally and aesthetically. Case report: A 50-year-old woman came with chief complaints of cavities in her lower front teeth. From subjective, objective, and radiographic examination, tooth 31 was diagnosed with irreversible pulpitis with normal apical tissue, and a root canal treatment was planned. In the next visit, the root canal treatment was carried out using the step-back technique starting from IAF K-File #10 and MAF K-File #25 with a working length of 22.5 mm. The final K-File used was K-File #40, with a working length of 19.5 mm. Root canal treatment on tooth 31 was carried out in 10 visits. After radiographic evaluation subjective and objective examination, the root canal treatment was completed, and a post-crown restoration was planned as a follow-up. Conclusion: Root canal treatment in 10 visits on tooth 31, which was diagnosed with irreversible pulpitis with normal apical tissue, carried out using the step-back technique in this case, showed clinical and radiological success.

AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

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