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All Journal Majalah Kedokteran Bandung Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry
Marry Siti Mariam
Jurusan Biologi Mulut Fakultas Kedokteran Gigi Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Antimicrobial properties of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis ATCC 29212 Sovira, Gita Dwi Jiwanda; Satari, Mieke Hemiawati; Mariam, Marry Siti
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.25206

Abstract

Introduction: Enterococcus faecalis is often found in root canal infections and can cause failure in root canal treatment. Uncaria gambier is an herbal medicine that is known to be rich in polyphenol compounds that have antibacterial properties. Because of the polyphenol content and antibacterial properties of Uncaria gambier, it can be an alternative as an antibacterial solution for root canal treatment. The purpose of this study is to analyze the antimicrobial of various solvents combinations for phytochemical fraction derived from Uncaria gambier extract against Enterococcus faecalis (ATCC 29212). Methods: This study is descriptive explorative research Uncaria gambier was extracted using a combination of three different fractions, ethyl acetate-water, hexane-water and hexane-ethyl acetate. We determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Enterococcus faecalis  (ATCC 29212) using the serial microdilution method and by add test samples to the Mueller Hinton agar medium from each fraction Results: Combination Ethyl Acetate-water has MIC value at 0.195 mg/ml, combination Hexane-water at 0.049 mg/ml and combination Hexane- Ethyl Acetate have the MIC value at 1.563 mg/ml. The MBC value in combination Ethyl Acetate-water at 25 mg/ml, combination Hexane-Ethyl Acetat and Hexane-water MBC value at 50 mg/ml and 100 mg/ml. Based on optical density measurement using ELISA Reader and bacterial growth on the media used, the fraction combinations increase the antibacterial effect of Uncaria gambier against E. faecalis ATCC 29212. Conclusion: The combination of various Uncaria gambier  solvents has been shown to inhibit and kill E. faecalis (ATCC 29212). The combination of Ethyl Acetate-water fraction is the best combination to against E. faecalis (ATCC 29212). It means that the combination of various Uncaria gambier solvents can be developed as alternative root canal irrigation.
The linear polymerisation shrinkage effect of chemical activated composite resin using different bonding agents on the dimensions of the tooth cavity Mariam, Marry Siti; Cahyanto, Arief
Padjadjaran Journal of Dentistry Vol 32, No 1 (2020): March 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol32no1.25915

Abstract

Introduction: This study was aimed to determine the effect of the linear polymerisation shrinkage of composite resin using different bonding agents on the dimension of the tooth cavity. Methods: Fifty-four extracted human premolar teeth, both maxillary and mandibular were used and divided into three groups (A, B, and C) into eighteen specimens. The conventional cavity was prepared, and all cavities were bevelled. In Group A, only the bevel was etched, and the enamel bond was applied to it. Group B was treated with the same procedure as group A, but the dentin bond was used instead of enamel bond. Group C, the whole cavity was etched, dentin bond was applied on the bevel and the cavity walls. The reference points were placed in the vestibular and lingual palatal region as occlusal as possible on the enamel. The Silar® composite resin (microfilled, chemically activated), etching agent (gel etch), enamel and dentin bond system were employed for the preparation and filing of the cavities. The cavity was filled with composite, and the specimens were immersed in 0.02% chlorhexidine gluconate solution at the room temperature. The distance between both reference points was measured before and after filling procedures. Results: Group A showed the least shrinkage with a mean of 0.14%, group B showed a larger shrinkage with the average of 0.24% and the largest shrinkage was found in group C with the average of 0.26%. The difference between groups B and C was not statistically significant given at the point of 0.05 significance level, and the difference between groups A, B, and C was significant at the 0.01 significance level. From the mathematical modelling using the stress-strain equation, the results showed that the tensile stress in restrained of Silar® composite was higher than the enamel (19.80 MPa > 10.34 MPa) thus lead to cracks in the enamel. Conclusion: The acid etching on dentin is not improving the retention and marginal adaption of restorative resin. The use of enamel bond system gives a better effect on the adhesion strength compared to the dentin bond system.
Co-Authors Amida, Aya Ayu Trisna Hayati, Ayu Trisna Cahyanto, Arief Denta Asnatasia Nurmadhini Deradjat, Arini Puspitasari Dudi Aripin Fitri Anggraini, Fitri Fourier Dzar Eljabbar Latief Gilang Yubiliana, Gilang Gita Dwi Jiwanda Sovira Imelda, Reza Afri Kosterman Usri, Kosterman Mieke Hemiawati Satari Nina Djustiana Ririn Ariyanti, Ririn Sri Tjahajawati, Sri Sunardhi Widyaputra Wazilah Nasserie Winny Yohana, Winny