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Journal : Indonesian Journal of Electrical Engineering and Computer Science

Feature analysis for stage identification of Plasmodium vivax based on digital microscopic image Hanung Adi Nugroho; I Md. Dendi Maysanjaya; Noor Akhmad Setiawan; E. Elsa Herdiana Murhandarwati; Widhia K.Z Oktoeberza
Indonesian Journal of Electrical Engineering and Computer Science Vol 13, No 2: February 2019
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v13.i2.pp721-728

Abstract

Plasmodium parasite is identified to confirm malaria disease.  Paramedics need to observe the presence of this parasite prepared on thick and thin blood films under microscope.  However, false identification still occurs which is caused by human factor during the examination.  Thus, malaria identification based on digital image processing has been widely developed to overcome the error possibility.  This paper proposes a scheme to identify and classify the stages of Plasmodium vivax parasite on digital microscopic image of thin blood films based on feature analysis.  Shape and texture features are extracted from segmented parasite objects.   Feature selection based on wrapper method is then conducted to obtain relevant features which may contribute in improving the classification result.  The classification process is conducted based on Naïve Bayes classifier.  The performance of proposed method is evaluated using 73 digital microscopic images of P.vivax parasite on thin blood films comprising of 29 trophozoites, 10 schizonts and 34 gametocytes stages.  By using six selected features including perimeter, dispersion, mean of intensity, ASM, contrast GLCM and entropy GLCM, the proposed scheme achieves the best classification rate with the accuracy, sensitivity and specificity of 97.29%, 97.30% and 97.30%, respectively.  This indicates that the proposed scheme has a potential to be implemented in the development of a computerised aided malaria diagnosis system for assisting the paramedics.
Identification of plasmodium falciparum and plasmodium vivax on digital image of thin blood films gf Hanung Adi Nugroho; Made Satria Wibawa; Noor Akhmad Setiawan; E. Elsa Herdiana Murhandarwati; Ratna Lestari Budiani Buana
Indonesian Journal of Electrical Engineering and Computer Science Vol 13, No 3: March 2019
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v13.i3.pp933-944

Abstract

Observing presence of Plasmodium parasite of stained thick or thin blood films through microscopic examination is a gold standard for malaria diagnosis.  Although the microscopic examination has been extensively used, misidentification might occur caused by human factors.  In order to overcome misidentification problem, several studies have been conducted to develop a computer-aided malaria diagnosis (CADx) to assist paramedics in decision-making.  This study proposes an approach to identify species and stage of Plasmodium falciparum and Plasmodium vivax on thin blood films collected from the Laboratory of Parasitology, Faculty of Medicine, Universitas Gadjah Mada.  Adaptive k-means clustering is applied to segment Plasmodium parasites.  A total of 39 features consisting of shape and texture features are extracted and then selected by using wrapper-based forward and backward directions.  Classification is evaluated in two schemes.  The first scheme is to classify the species of parasite into two classes. The second scheme is to classify the species and stage of parasite into six classes.  Three classifiers applied are k-nearest neighbour (KNN), support vector machine (SVM) and multi-layer perceptron (MLP).  Furthermore, to facilitate the multiclass classification, one-versus-one (OVO) and one-versus-all (OVA) methods are implemented.  The first scheme achieves the accuracy of 88.70% based on MLP classifier using three selected features.  While the accuracy gained by the second scheme is 95.16% based on OVO and MLP classifier using 29 selected features.  These results indicate that the proposed approach successfully identifies the species and stage of parasite on thin blood films and has potential to be implemented in the CADx system for assisting paramedics in diagnosing malaria.