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All Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Jurnal Ilmiah Kesehatan Jurnal Ilmu Kefarmasian Indonesia Bioscientist : Jurnal Ilmiah Biologi Journal Borneo Jurnal Farmasindo: Jurnal Penelitian dan Pengabdian Masyarakat JI-KES (Jurnal Ilmu Kesehatan)
Rukaya, Benazir Evita
Politeknik Kaltara
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Toxicity test of Kedayan root infusion (Aristolochia sp.) using brine shrimp lethality test method Syuhada, Syuhada; Rukaya, Benazir Evita; Setiawan, Budi
Media Farmasi: Jurnal Ilmu Farmasi Vol. 19 No. 2: September 2022
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v19i2.21045

Abstract

Kedayan roots are still used by the Lundayeh Dayak people as traditional medicine. This plant is one of the Aristolochia sp. species whose production and distribution as traditional medicine or food supplements are prohibited by the Food and Drug Supervisory Agency of the Republic of Indonesia because it has side effects that are harmful to the body. The purpose of this study was to determine the toxicity of kedayan root infusion using the Brine Shrimp Lethality Test (BSLT) method based on the LC50 value. Toxicity test was carried out by varying the concentration of Kedayan root infusion, namely 100, 500, and 1000 ppm, as an intervention against Artemia salina Leach with a negative control in the form of 2.8% saline solution for 24 hours with 3 replications, then observing the presence or absence of movement of Artemia salina L. Larvae. Test results data in the form of % mortality obtained, then analyzed using the probit regression analysis method to determine the LC50 value. The results of this study indicate that the kedayan root infusion has very high toxicity, the LC50 value is 101.9278 ppm.
Evaluasi Aktivitas Antibakteri Minyak Jintan Hitam Terhadap Bakteri Gram-Positif dan Gram-Negatif: Studi pada Staphylococcus aureus dan Pseudomunas aeruginosa Salsabila, Maisan Khoiri; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14950

Abstract

This study aims to evaluate the antibacterial activity of black cumin oil (Nigella sativa) against Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria. Methods: The antibacterial activity was tested using the well diffusion method with varying concentrations of black cumin oil (0.1%, 0.5%, 1%, and 2%), along with a negative control using ethyl acetate as the solvent. Additionally, a simple phytochemical screening was conducted using a test tube reaction method to detect the presence of active compounds such as alkaloids, flavonoids, tannins, and saponins. The results showed that (1) black cumin oil at a concentration of 2% produced the largest inhibition zones, measuring 15.33 ± 3.21 mm for Staphylococcus aureus and 9.37 ± 1.98 mm for Pseudomonas aeruginosa; (2) Statistical analysis confirmed significant differences in several treatments, with a p-value < 0.05. The antibacterial effect of black cumin oil was stronger against Gram-positive bacteria than Gram-negative bacteria, likely due to differences in bacterial cell wall structures; (3) Furthermore, black cumin oil tested positive for alkaloids and saponins, which are known as potential antimicrobial compounds. Overall, this study indicates that black cumin oil has the potential to serve as an effective natural antibacterial agent, particularly against Gram-positive Staphylococcus aureus.
Efektivitas Salep Minyak Jintan Hitam (Nigella sativa) sebagai Antibakteri terhadap Staphylococcus aureus dan Pseudomonas aeruginosa Arifullah, Muhamad Aqsal; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15005

Abstract

This study aimed to evaluate the effectiveness of black cumin oil ointment as an antibacterial agent against Staphylococcus aureus and Pseudomonas aeruginosa. The ointment was formulated with three variations of black cumin oil concentrations (0.1%, 1%, and 10%) using a lipophilic base. The physical stability test was conducted by evaluating organoleptic properties, homogeneity, pH, spreadability, and adhesion. The antibacterial activity was tested using the well-diffusion method against Staphylococcus aureus and Pseudomonas aeruginosa and analyzed using the One Way ANOVA method followed by a post hoc Tukey test. The results of this study indicate that (1) The black cumin oil ointment demonstrated good physical stability, particularly in terms of homogeneity and pH; (2) The antibacterial activity test showed that the ointment with a 10% black cumin oil concentration (F3) effectively inhibited the growth of Staphylococcus aureus with an efficacy not significantly different from gentamicin ointment (p = 0.477), but significantly different from the negative control (p = 0.027). However, no antibacterial activity was observed against Pseudomonas aeruginosa.Conclusion: The 10% black cumin oil ointment formulation has the potential as a natural antibacterial agent against Staphylococcus aureus. These findings suggest that black cumin oil can be further developed as a natural-based topical therapy alternative for treating skin infections caused by Gram-positive bacteria.
Uji Cemaran Mikroba pada Jajanan Pedagang Kaki Lima Sekolah Dasar di Kecamatan Tarakan Tengah Menggunakan Metode Angka Lempeng Total Nurfadilah, Nurfadilah; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15026

Abstract

This study aims to evaluate the level of microbial contamination in street food sold and to conduct direct observations on environmental sanitation and hygiene practices carried out by street vendors in elementary school environments in Kecamatan Tarakan Tengah. The microbial contamination levels in 15 research samples, consisting of 9 types of food and 6 types of beverages, were randomly obtained from five different sub-districts and measured using the Total Plate Count (TPC) method. During the sampling process, observations and documentation of activities and conditions around the sales locations were conducted. The results of this study indicate that (1) all samples had TPC values far exceeding the limits set by BPOM, with the highest contamination found in pentol ayam goreng (8.69 × 10¹⁰ CFU/g) and es nutrijel stroberi (4.79 × 10¹⁰ CFU/ml); (2) The main factors contributing to the high microbial contamination, based on direct observations during sampling, include poor sanitation practices, exposure to an open environment, and the use of unhygienic equipment. Therefore, it can be concluded that street food sold in the vicinity of elementary schools in Kecamatan Tarakan Tengah presents a significant health risk to students due to the high level of microbial contamination.
Studi Mikrobiologi pada Jajanan Kaki Lima di Sekolah Dasar Kecamatan Tarakan Barat: Implikasi bagi Keamanan Pangan Sholehah, Nur Ulya Aufa; Rukaya, Benazir Evita; Syuhada, Syuhada
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15038

Abstract

This study aims to evaluate the microbiological safety of street food sold by vendors in Tarakan Barat District. The evaluation was conducted on 12 food samples, consisting of 8 types of food and 4 types of beverages, randomly collected from vendors in four sub-districts: Karang Anyar, Karang Balik, Karang Rejo, and Karang Anyar Pantai. Samples were tested using the Total Plate Count (ALT) method to determine the bacterial colony count in the tested food and beverages. The results showed that (1) the sample with the highest contamination level among food items was Pentol Rebus/Kukus (3,19 × 10¹⁰CFU/g), while the beverage with the highest ALT was Es Teh Sisri (I) (2,59 × 10¹⁰ CFU/ml) obtained from SDN 003 Tarakan, whereas Es Teh Sisri (D) from SDN 1 Utama Tarakan showed a TFTC (Too Few to Count) result; (2) The high microbial contamination levels in these street foods are suspected to be caused by several factors, including poor environmental sanitation, lack of vendor hygiene, and exposure to dust and pollution from the selling locations. Most of the tested samples had ALT levels far exceeding the limits set by BPOM, confirming that street food poses a risk of causing various diseases such as gastrointestinal infections, diarrhea, food poisoning, and typhoid fever, which may negatively impact the health of elementary school students in Tarakan Barat District.
Optimasi Sterilisasi Minuman Jamu Kunyit-Asam Kemasan dengan Metode Pasteurisasi Putri, Meilani; Syuhada, Syuhada; Rukaya, Benazir Evita
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.15042

Abstract

This study aims to evaluate the effect of pasteurization at 65°C and 85°C on pH stability, organoleptic characteristics, and microbiological contamination of turmeric and tamarind herbal medicine during 15 days of storage. This research is an experimental study in the laboratory. The research methods include pasteurization at the two temperatures, pH testing, organoleptic tests (including colour, aroma, and taste), and total plate count (TPC) analysis to assess microbial contamination. The results of the study showed several main findings (1) pasteurization was effective in maintaining the stability of pH, colour, and aroma of turmeric and tamarind herbal medicine during 15 days of storage; (2) there was an increase in flavour intensity on the 15th day in the pasteurized group; (3) pasteurization at 85°C reduced the lowest microbial contamination rate (1.85 × 10¹⁰ CFU/mL) compared to the group without pasteurization (3.33 × 10¹⁰ CFU/mL). Thus, pasteurization in this study increased microbiological safety and affected the sensory characteristics of turmeric and tamarind herbal medicine, especially in taste intensity.
Optimasi dan Uji Efektivitas Gel Shampoo Antiketombe Poliherbal terhadap Jamur Penyebab Ketombe Rukaya, Benazir Evita; Syuhada
JI-KES (Jurnal Ilmu Kesehatan) Vol. 8 No. 2 (2025): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : LPPM Universitas Hafshawaty Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/jikes.v8i2.869

Abstract

Abstrak Ketombe adalah masalah utama bagi sebagian besar masyarakat di dunia. Kemunculan ketombe biasanya dipengaruhi oleh adanya infeksi jamur Malassezia furfur yang mempengaruhi estetika dan sering menimbulkan rasa gatal. Penelitian ini bertujuan untuk memperoleh sediaan gel shampoo poliherbal dengan konsentrasi kombinasi ekstrak seledri, bawang dayak dan daun sirih yang optimal dalam menghambat pertumbuhan jamur Malassezia furfur. Metode penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental in vitro, yang diawali dengan proses maserasi menggunakan pelarut etanol 70% untuk masing-masing tanaman, kemudian diuapkan untuk mendapatkan ekstrak kental. Ekstrak diformulasikan menjadi 3 variasi konsentrasi kombinasi (daun seledri: bawang dayak: sirih) dengan perbandingan (2:1:3);(3:2:1); dan (1:3:2) dan dilakukan uji pendahuluan, selanjutnya diformulasikan menjadi sediaan gel shampoo dan dilakukan evaluasi stabilitas fisik sediaan. Selain itu, juga dilakukan pengujian aktivitas antijamur lanjutan dengan 3 kelompok uji. Hasil penelitian menunjukkan bahwa perbandingan konsentrasi yang optimal adalah F3 (1:3:2),  shampoo poliherbal (F3) memiliki aktivitas antijamur sebesar 24,20 mm. Shampoo ini juga menunjukkan stabilitas busa yang baik dan pH yang sesuai dengan standar produk perawatan kulit. Kesimpulan, shampoo poliherbal yang mengandung kombinasi ekstrak seledri, bawang dayak, dan daun sirih memiliki potensi sebagai produk perawatan kulit dengan aktivitas antijamur yang efektif dan stabil, serta aman digunakan. Kata kunci: antijamur, bawang dayak, gel shampoo, sirih, seledri   Abstract Dandruff is a common issue for many people worldwide. Its appearance is often associated with the fungal infection Malassezia furfur, which affects aesthetics and frequently causes itching. This study aimed to develop a polyherbal gel shampoo formulation with an optimal concentration of celery, Dayak onion, and betel leaf extracts to inhibit the growth of Malassezia furfur. The research method used in this study was an in vitro experimental approach, starting with the maceration process using 70% ethanol as a solvent for each plant, followed by evaporation to obtain a concentrated extract. The extracts were formulated into three different combination concentration variations (celery: Dayak onion: betel leaf) with ratios of (2:1:3), (3:2:1), and (1:3:2), followed by preliminary testing. The formulations were then developed into gel shampoo preparations and subjected to physical stability evaluations. Additionally, antifungal activity testing was conducted with three test groups. The results showed that the optimal concentration ratio was F3 (1:3:2), where the polyherbal shampoo (F3) demonstrated antifungal activity of 24.20 mm. This shampoo also exhibited good foam stability and a pH that aligns with skincare product standards. In conclusion, polyherbal shampoo containing a combination of celery, dayak onion, and betel leaf extracts has potential as a skin care product with effective and stable antifungal activity, and is safe to use. Keywords: antifungal, dayak onion, gel shampoo, betel leaf, celery
Co-Authors Adriansyah Arjuna Ainul Rabiah Akbar Syahputra Arifullah, Muhamad Aqsal Ayu Febianah Ayu Puspasari Benyamin Budi Setiawan Budi Setiawan Devy Yulia Veronika Dewi Puspita Sari Didin Sampurno Aji Faizal Mustamin Fajar Wahyu Ferry Fahruddin Hendriansyah Hendriansyah Heriani Heriani Ilham Ilham Indah Lestari Indriawati Indriawati Jumsina Jumsina Kintoko Kintoko Kintoko, Kintoko Monika Limbong Nadillah Nining Sugihartini Noor Hadızah Nurfadilah Nurfadilah Putri, Meilani Risal Hidayat Salsabila, Maisan Khoiri Salsabyla Asky Sari Wijayanti Sholehah, Nur Ulya Aufa Syarifah Nur Fauziah Syuhada syuhada Syuhada Syuhada Syuhada Syuhada Tahuti Mustika Yulianti Clarisa Febrilia