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THE CLINICAL EPIDEMIOLOGY OF NOROVIRUS INFECTION IN CHILDREN WITH DIARRHEA AT REGIONAL PUBLIC HOSPITAL DR.SOETOMO Alpha Fardah Athiyyah; Sofia Wardhani; Andy Darma; Reza Gunadi Ranuh; Dadik Raharjo; Toshiro Shirakawa; Subijanto Marto Sudarmo
Jurnal Berkala Epidemiologi Vol. 8 No. 3 (2020): Jurnal Berkala Epidemiologi (Periodic Epidemiology Journal)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbe.V8I32020.200-207

Background: Norovirus has become a major cause of severe outbreaks of gastroenteritis since the discovery of the rotavirus vaccine, with the main symptom being diarrhea. Until now, research on the epidemiological analysis of norovirus has not been carried out at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya. Purpose: This study aims to provide clinical epidemiology data and an analysis of norovirus infections in children with diarrhea at Regional Public Hospital (RSUD) Dr. Soetomo, Surabaya, Indonesia, including the prevalence of norovirus infection in each age group and sex, its clinical appearance, and its seasonal variation. Methods: A cross-sectional study was conducted in children aged 1–60 months hospitalized for diarrhea in RSUD Dr. Soetomo between April 2013 and March 2014. Identification of the virus in the stool was done by norovirus enzyme immunoassay Quick NaviTM Noro2. The proportion, age, sex, clinical symptoms, and patterns of the norovirus seasonal data were calculated. Results: Norovirus was detected in 64 samples (19%) of the 340 stool samples, with a mean patient age of 11.75 months; it was mostly found in patients less than 24 months of age (95%), and 64% were male. The monthly pattern of norovirus infection was mostly found in November, followed by May and April. The clinical symptoms were fever (72%), vomiting (66%), bloating (59%), abdominal cramps (34%), perianal inflammation (27%), abdominal distension (16%), and seizures (8%). Conclusion: The prevalence of norovirus was found to be high in patients with diarrhea aged 1–60 months hospitalized in RSUD Dr. Soetomo, with a proportion of 19%. Further research is needed to determine the severity of norovirus infection.

Occurrences Salmonella sp. and Escherichia Coli in Bulk and Packaged Chicken Sausages in Surabaya, Indonesia Deana Fyra Adi Nur; Ratna Yulistiani; Dedin F. Rosida; Dadik Raharjo
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 6 No. 2 (2022)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Salmonella sp. and Escherichia coli are pathogenic bacteria that cause foodborne diseases that often contaminate food and are harmful to human health. Sales of unpackaged (bulk) sausages accompanied by poor hygiene and environmental sanitation conditions have a high potential for Salmonella and Escherichia coli contamination. The purpose of this study was to compare the contamination level of bacterial, Salmonella sp. and Escherichia coli in bulk and packaged chicken sausages as well as to determine the relationship between the hygiene and sanitation of traders and the level of bacterial contamination, Salmonella sp. and Escherichia coli contamination in chicken sausages sold at traditional markets in Surabaya, Indonesia. This research is a cross-sectional study and purposive sampling method. The results showed that the level of bacterial contamination in the bulk chicken sausage was higher (5.98 Log CFU/g) than in packaged chicken sausage (4.83 Log CFU/g). Salmonella sp. contamination in the bulk chicken sausage was higher (44.44%) than in packaged chicken sausage (10.00%) and Escherichia coli contamination in the bulk chicken sausage was higher (22.22%) than in packaged chicken sausage (20.00%). There is a significant relationship between the hygiene and sanitation of traders with the contamination level of bacteria, Salmonella sp., and Escherichia coli in bulk and packaged chicken sausages.

Tingkat cemaran bakteri Coliform dan Escherichia coli pada makanan dan minuman sebagai dampak kondisi higiene sanitasi di Sentra Kuliner Penjaringansari, Surabaya Ratna Yulistiani; Jariyah; Dadik Raharjo; Ulya Sarofa; Dinda Alvianita Sabrina

Publisher : Program Studi Ilmu dan Teknologi Pangan, Universitas Yudharta, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/tp.v14i1.3565

???????????? ???????????????????????????????? ???????? ???????????????????????????????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ???????? ???????????????? ???????? ???????????????????????????????????? ???????? ???????? ???????????????????????????????????? ???????? ???????????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????? ???????????????? ???????????????????????????? ????????????????????????????????????. ???????????????? ???????????????????????????????? ???????? ???????? ???????????????????????????????????????????????????? ????????????????????, ???????????????????????????? ???????????????? ???????????????? ???????????????? ???????????? ???????????????????????????????????? ???????????????? ???????? ???????????????????????????? ???????? ???????????? ???????????????????????????????????????????? ???????????????? ???????????????????????????????? ???????????????????????? ???????? ????????????????????????????????. ???????????? ???????????????? ???????? ???????????????? ???????????????????? ???????????? 1). ???????????????????????????????? ???????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????????????????????????????????? ???????? ???????????????? ???????????????????????????? ????????????????????????????????????; 2). ???????????????????????????????????? ???????????? ???????????????? ???????????????????????? ???????? ???????????????? ???????????? ???????????????????????????????? ???????????????????????????????? ???????? ???????????????????? ???????? ???????????? ???????????????????????????????????? ???????????????????????????????????????????????????? ????????????????????, ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ????????????????????????????????????????????????????; 3). ???????????????????????????? ???????????? ???????????????????????????????????????????????? ???????????????????????????? ???????????? ???????????????????????????? ???????????? ???????????????????????????????????????? ???????? ???????????????????????????? ???????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ????????????????????????????????????????????????????; 4). ???????????????????????????????? ???????????????????????????? ???????? ???????????????????????????????????? ????????????????????????????????????????????????????. ???????????? ???????????????????????????? ???????????? 41.67% ???????? ???????????????? ???????????????????????????? ???????????????????????????????????????? ???????????????????? ???????????????????????????????? (???????????????????? 2.7 ???? 104 -3.8 ???? 105 ????????????????????????????????/????????????????) ???????????????????????????????????? ???????????? ???????????????????????????? ???????????????????? ???????? ????????????????. ???????????????????????????????? ???????????????????????????? ???????????????????????? ???????????????? 100% ???????? ???????????????? ???????????????????????????? ???????????? 66.6% ???????? ???????????????????????? ???????????? ???????????????????????? ???????????????????????????? ???????????? ???????????????????????????????? ???????????? ???????????????????????????? ???????????????????? ???????? ???????????????? ???????????????????????????????????????????????? (???????????????? ???????????? ???????????????????? ???????????????????????????? >3 ????????????/????; ???????????????????????? > 0 ????????????/????) ???????????? 50% ???????? ???????????? ???????????????????????????? ???????????????????????????????????? ???????????????????????????????????????????? ???????????????? ???????????????????????? ???????????????? ????????????????????????????????????. ???????????????????? ???????? ???? ???????????????????????????????????????????? ???????????????????????????????????????????????? (????<0.05) ???????????????????????????? ???????????? ???????????????????????????????????????? ???????????????????????????? ???????? ???????????????????????????? ???????????????? ???????????????????? ???????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????? ???????????????????????????????????????????????????? ???????? ????????????????, ???????????????????????????????????? ???????????? ????????????????????????. ???????????????????????????? ????????????????????????????????, ????????????????????????, ???????????? ???????????????????? ???????????? ???????????? ???????????????????? ???????? ???????????????????????????????? ???????????? ???????????????????????????? ???????? ???????????????????????????????? ????????????????????????????????????????????????????.

Logo, Label, and Packaging Improvement on Jamu Bu Uri Products for Digital Marketing Kartini; Ratna Yulistiani; Dadik Raharjo; Rizky Fatkhur Rohman; Sri Alam Syah Lihan Candra Asi
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3342

It is quite common knowledge that some traditional herbal medicine sellers used plastic bottles of mineral water as a storage medium for their traditional herbal medicine. Even though the allotment of mineral water bottles is dispossible, it cannot be used again after the contents run out. This problem about the packaging must be addressed. When viewed from the visual aspect, the presentation of traditional herbal medicine still tends to be monotonous in terms of packaging. Whereas packaging can influence consumers to give a positive response, good packaging will optimize profits. Therefore, this service activity targets several improvements in handling packaging problems for Jamu bu Uri products, with the aim of increasing the marketing of traditional herbal products.

The Quality of Milk Production in Friesian Holstein (FH) Dairy Cattle Experiencing Repeat Breeding at KUD Tani Wilis Sendang, Tulungagung Regency Sintya Kumalasari Wibowo; Aldin Akbar Rahmatullah; Cindy Ercha Aulia Putri; Pudji Srianto; Dian Ayu Permatasari; Dadik Raharjo; Rimayanti; Erma Safitri; Mohammad Auzaie Afandi; Nuurin Ajrin Karim
Journal of Applied Veterinary Science And Technology Vol. 5 No. 2 (2024): October 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I2.2024.135-139

Background: Repeat breeding is a notable reproductive problem where cows do not conceive after several insemination attempts, and it is typically defined as three or more unsuccessful attempts. This results in reduced reproductive efficiency, lower economic sustainability of the dairy farm, and decreased milk quality in dairy cattle. Purpose: The purpose of this study was to investigate changes in milk quality in Friesian Holstein (FH) dairy cows subjected to repeat breeding at KUD Tani Wilis Sendang, Tulungagung Regency, focusing on the milk’s density, as well as fat, Total Solid (TS), and Solid Non-Fat (SNF) content. Method: Thirty-two milk samples were collected, including 10 from normal cows for comparison. The samples were analyzed using a Lactoscan to examine the density, fat content, total solid (TS), and solid non-fat (SNF) of milk. The data was subjected to Independent T-Test analysis. Results: The results revealed significant differences (p<0.05) between repeat breeding and normal cow milk samples in terms of specific gravity, fat content, and Total Solids (TS), but not in Solid Non-Fat (SNF). Repeat breeding cow milk exhibited a higher specific gravity (1.0282 vs. 1.0260) but lower fat content (1.08% vs. 4.18%) and Total Solids (9.38% vs. 12.73%) compared to normal cow milk. However, there was no significant difference for Solid Non-Fat (8.36% for repeat breeding vs. 8.55% for normal cows). Conclusion: Repeat breeding cow milk showed increased specific gravity but decreased fat content and Total Solids compared to normal cow milk, while Solid Non-Fat remained relatively consistent.

Logo, Label, and Packaging Improvement on Jamu Bu Uri Products for Digital Marketing Kartini; Ratna Yulistiani; Dadik Raharjo; Rizky Fatkhur Rohman; Sri Alam Syah Lihan Candra Asi
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3342

It is quite common knowledge that some traditional herbal medicine sellers used plastic bottles of mineral water as a storage medium for their traditional herbal medicine. Even though the allotment of mineral water bottles is dispossible, it cannot be used again after the contents run out. This problem about the packaging must be addressed. When viewed from the visual aspect, the presentation of traditional herbal medicine still tends to be monotonous in terms of packaging. Whereas packaging can influence consumers to give a positive response, good packaging will optimize profits. Therefore, this service activity targets several improvements in handling packaging problems for Jamu bu Uri products, with the aim of increasing the marketing of traditional herbal products.

Detection of csg and lux Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections Rini Purbowati; Sri Lestari Utami; Dadik Raharjo; Masfufatun Masfufatun
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 1 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.222

Uropathogenic Escherichia coli (UPEC) is responsible for 80–90% of urinary tract infections (UTI) in the global population. The emergence of the increasing resistance to broad-spectrum antimicrobial agents was due to the ability to form biofilms. Cell surface factors that play a role in biofilm formation include Quorum Sensing (QS) which is encoded by the luxS family gene and curli by two operons, namely the csgBA operon. The purpose of the study is to detect the effects of 2 virulence genes (csgD and luxS) on biofilm-forming UPEC associated with UTI. As many as 76 UPEC isolates were collected from the clinical microbiology laboratories and the biofilm development was analyzed using the crystal violet method on microplate 96 wells. Using PCR assay, the two studied genes (csgD and luxS) were determined to be present in the isolates. UPEC isolates the bacteria-produced biofilms (90.80%) and nonproducers (9.20%). Most UPEC bacteria (97.36%) are known to be positive for csgD and luxS gene, while the others (92.10%) are known to be positive for the luxS gene. The highest proportion of the genes expressed in this study is followed by the presence of a relationship between the ability to produce biofilm and the presence of the genes under investigation, which is followed by all UPEC strains that cause UTI in humans.

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