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Prevalensi dan Analisis Faktor Risiko Multidrug Resistance Bakteri Escherichia coli pada Ayam Komersial di Kabupaten Blitar Wibisono, Freshinta Jellia; Sumiarto, Bambang; Untari, Tri; Effendi, Mustofa Helmi; Permatasari, Dian Ayu; Witaningrum, Adiana Mutamsari
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Multidrug resistance is a problem that is difficult to overcome in terms of treating infectious diseases. Multidrug resistance is the term used to describe when a bacteria is resistant to three or more different classes of antibiotics. Escherichia coli as a commensal bacterium which has multidrug resistance, this causes more issues because Escherichia coli can transfer its resistant properties to other bacteria within the poultry digestive tract. The observational study is used to determine the risk factors and to estimate the quantitative effects arising from various components that contribute to the emergence of a disease. The sampling in this study was carried out randomly through cloaca swabs from commercial chicken farms in Blitar and 345 samples were collected. Complementary data collection was carried out using questionnaires, interviews, and field observations. The results showed the incidence of multidrug resistance in commercial chickens in the Blitar District was 72.5%. There is a relationship between causative factors with the incidence of multidrug resistance in Escherichia coli bacteria that is significantly associated with positive risk factors. The strength of this relationship can be seen from the value of OR and RR, among others factors of chicken breed (OR = 3.07; RR = 1.34), breeder's education (OR = 2.3; RR = 1.29), type of livestock business (OR = 7.5; RR = 1.43), type of animal feed (OR = 1.91; RR = 1.2), veterinary support for livestock raising management (OR = 3.09; RR = 1.44). The reference variables are whether the antibiotics are administered by non-veterinarians (OR = 2.35) or by the TS (OR = 7.92), and whether there is an antibiotic administration program (OR = 3.16; RR = 1.47). The overseeing function of farm maintenance, management, and implementation of antimicrobial administration in commercial chicken farms needs to be improved, to increase breeders' awareness of the careful usage of antibiotics and controlling the incidence of antibiotic resistance.

Pemodelan Epidemiologi Kejadian Multidrug Resistance Bakteri Escherichia coli pada Peternakan Ayam Komersial di Kabupaten Blitar Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.52071

Sifat resistensi bakteri Escherichia coli terhadap antibiotik mengakibatkan terbatasnya pilihan pengobatan. Perkembangan lebih lanjut dari resistensi bakteri dapat menyebabkan munculnya multidrug resistance pada bakteri, sehingga meningkatkan morbiditas dan mortalitas penyakit. Interaksi penyebaran kejadian multidrug resistance pada Escherichia coli yang terjadi pada populasi sangat kompleks, sehingga sulit memahami dinamika penyebaran berskala besar. Pendekatan pemodelan menjadi sangat penting untuk pengambilan keputusan tentang program pengendalian penyakit infeksi. Penelitian ini merupakan penelitian epidemiologi deskriptif analitik dengan desain cross-sectional study. Metode analisis menggunakan analisis regresi logistic untuk mendapatkan pemodelan kejadian multidrug resistance bakteri Escherichia coli pada tingkat ternak, dan menggunakan regresi linier untuk mendapatkan pemodelan kejadian multidrug resistance bakteri Escherichia coli pada tingkat peternakan. Hasil dari penelitian ini menunjukkan Distribusi kasus kejadian multidrug resistance pada ayam komersial di Kabupaten Blitar menunjukkan prevalensi kejadian pada tingkat peternakan sebesar 95.9%. Pemodelan kejadian multidrug resistance bakteri Escherichia coli tingkat ternak menghasilkan model regresi logistik ganda Ln () = 0.21964 + 1.60374 RefTS + 1.44989 Broiler + 0.96022 PakRacik + 0.84182 ProgAb – 1.16667 SaniKan – 1.15046 Tritendap, dengan peluang kejadian sebesar 94 %. Pemodelan kejadian Multidrug resistance bakteri Escherichia coli tingkat peternakan menghasilkan model regresi linier, MDR (Y) = 0.57886 + 0.16105 JUMitra + 0.19342 ProgAb – 0.16178 Dukudrh. Model ini memiliki wilk saphiro mendekati 1 (W = 0,9573) sehingga model persamaan ini merupakan model yang baik untuk kejadian Multidrug resistance bakteri Escherichia coli tingkat peternakan.

Resistance Profile of Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria using Vitek® 2 Compact Method Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.51347

This study aimed to determine the resistance profile and the nature of multidrug resistance in Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E.coli) against several classes of antibiotics. Positive isolates of ESBL-producing E.coli were tested for antibiotic sensitivity using the VITEK® 2 compact method which then analyzed automatically. The results showed an antibiotic resistance profile against ESBL-producing E.coli showed the highest level of antibiotics in beta lactam, amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone at 100%. Subsequent results found a relatively high level of resistance in the antibiotics aztreonam (86.36%), trimethoprim/sulfamethoxazole (77.27%), gentamicin (72.73%), and ciprofloxacin (68.18%). Antibiotics from carbapenem groups such as ertapenem and memenem, and antibiotics from the aminoglycosides (amicasin) and tigecycline groups of tetracycline still showed a high sensitivity level of 100%. The most common resistance patterns found in ESBL-producing E.coli isolates are AM/AMP/KZ/CTX/CRO/ATM/GM/CIP as much as 22.73%, and AM/AMP/KZ/CTX/CRO/ATM/GM/CIP/SXT patterns of 18.2%. The results of multi-class antibiotic resistance showed that 86.36% had multidrug resistance. The highest multidrug resistance pattern in ESBL-producing E.coli occurred with a BL/AG/Q/SP pattern of 50%. Other patterns of multidrug resistance in ESBL-producing E.coli that can be found in this study are, the BL/AG/Q/SP pattern is 18.20%, the BL/AG/Q/SP pattern is 13.64%, and the BL/AG/Q pattern is 4.55%. The high profile of resistance and the nature of multidrug resistance in ESBL-producing E.coli has the potential to spread these resistant genes, thus risking the use of antibiotics as a public health therapy and animal health, therefore further evaluation and control are needed.

PENINGKATAN SUMBER DAYA PANGAN ASAL HEWAN DI KECAMATAN KANDANGAN KABUPATEN KEDIRI Adiana Mutamsari Witaningrum; Dhandy Koesoemo Wardhana; Dian Ayu Permatasari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 2 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i2.2020.243-250

Food of animal origin is used for human consumption such as meat, milk, eggs and their derivatives. Food safety for food of animal origin is needed to prevent biological, chemical and physical contamination that can interfere of human health. One of the animal-based food businesses that are mostly managed in the Kandangan, Kediri is milk. Activities carried out in this community service program are counselling and demonstrations related to increasing food resources of animal origin in the area. The community was able to understand this extension activity on the importance of increasing food resources of animal origin and was able to participate in demonstrations of making yogurt. The results of the pre-test and post-test on public knowledge and awareness of the evaluation of the delivery that have been given show an increase from an average of 89.1 points to 100 points. This activity is able to increase the knowledge and skills of the local community on how to increase animal-based food resources in order to develop their business.Pangan asal hewan dimanfaatkan untuk konsumsi manusia yang berupa daging, susu, telur dan hasil turunannya. Keamanan pangan pada pangan asal hewan diperlukan untuk mencegah cemaran biologis, kimia dan fisik yang dapat mengganggu kesehatan manusia. Salah satu usaha pangan asal hewan yang banyak dikelola di daerah Kecamatan Kandangan, Kabupaten Kediri yaitu susu. Kegiatan yang dilaksanakan dalam program pengabdian masyarakat ini berupa penyuluhan dan demonstrasi terkait peningkatan sumber daya pangan asal hewan di Kecamatan, Kabupaten Kediri. Masyarakat mampu memahami kegiatan penyuluhan ini akan pentingnya peningkatan sumber daya pangan asal hewan dan mampu mengikuti demonstrasi pembuatan yoghurt. Hasil pre-test dan post-test pada pengetahuan dan kesadaran masyarakat akan evaluasi dari penyampaian yang telah diberikan menunjukkan peningkatan dari rata-rata mendapatkan 89.1 poin menjadi 100 poin. Kegiatan ini mampu meningkatkan pengetahuan dan keterampilan masyarakat sekitar bagaimana cara meningkatkan sumber daya pangan asal hewan dalam rangka mengembangkan usahanya.

Prevalensi dan Derajat Infeksi Cacing Saluran Pencernaan pada Itik Jawa (Anas javanica) di Dua Daerah Geografis Berbeda Dian Ayu Permatasari; Kusriningrum Rochiman; Tjuk Imam Restiadi; Sri Mumpuni Sosiawati; Endang Suprihati; Mustofa Helmi Effendi
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determined the prevalence and infection disease degree of worm gastrointestinal on duck in two different geographical territories. The duck feces examination samples were using natif method, sedimentation, and floatation. The result of this examination of lowlands showed that 20% (12/60) ducks checked that infected by two worm species of nematoda, wich ia Ascaridia galli (11,7%), Heterakis sp. (8,3%), with the range of infection degree classified as low infection (0-500 EPG), while the examination results of highlands showed that 44% (33/75) ducks checked infected by two worm species of nematoda, which is Ascaridia galli (17,3%), Heterakis sp. (10,7%) and one species of trematoda, which is Echinostoma sp. (6,7%), with the range infection degree classified low infection (0-500 EPG). it can get both single or mix infection. The analysis result has shown that there was differences between lowlands and highlands on the prevalence and infection disease degree of worm gastrointestinal on duck, was highly significant (p<0,05).

Identification and Prevalence of Blood Protozoa on Culling Layer Hen Sold in Wonokromo Traditional Market Surabaya City Moch. Rizky Darmawan; Poedji Hastutiek; Arimbi Arimbi; Sunaryo Hadi Warsito; Dian Ayu Permatasari; Ririn Ririn
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The most common types of blood protozoa in chickens are from the phylum of Apicomplexa, including Plasmodium, Leucocytozoon and Haemoproteus. Blood protozoan diseases can cause decreased egg production, stunting and death in poultry farms. This study aims to identify and calculate the prevalence of blood protozoan infection in casted off laying chickens sold at Wonokromo Market, Surabaya City. One hundred blood samples from casted off laying chickens were taken by purposive sampling method. Blood samples were collected in Ethylene Diamine Tetra Acetate (EDTA) tubes, and then taken to the Parasitology Laboratory to make a blood smear which was then examined using a microscope with a magnification of 1000x. The results showed that the single infection was 43% positive for Plasmodium sp., 3% positive for Leucocytozoon sp. and 3% for mixed infections (Plasmodium sp. and Leucocytozoon sp.), with a total prevalence of positive samples infected with blood protozoa was 49%.

Prevalensi dan Analisis Faktor Risiko Multidrug Resistance Bakteri Escherichia coli pada Ayam Komersial di Kabupaten Blitar: Prevalence and Risk Factors Analysis of Multidrug Resistance of Escherichia coli Bacteria in Commercial Chicken, Blitar District Freshinta Jellia Wibisono; Bambang Sumiarto; Tri Untari; Mustofa Helmi Effendi; Dian Ayu Permatasari; Adiana Mutamsari Witaningrum
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 10 No. 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v10i1.74

Multidrug resistance is a problem that is difficult to overcome in terms of treating infectious diseases. Multidrug resistance is the term used to describe when a bacteria is resistant to three or more different classes of antibiotics. Escherichia coli as a commensal bacterium which has multidrug resistance, this causes more issues because Escherichia coli can transfer its resistant properties to other bacteria within the poultry digestive tract. The observational study is used to determine the risk factors and to estimate the quantitative effects arising from various components that contribute to the emergence of a disease. The sampling in this study was carried out randomly through cloaca swabs from commercial chicken farms in Blitar and 345 samples were collected. Complementary data collection was carried out using questionnaires, interviews, and field observations. The results showed the incidence of multidrug resistance in commercial chickens in the Blitar District was 72.5%. There is a relationship between causative factors with the incidence of multidrug resistance in Escherichia coli bacteria that is significantly associated with positive risk factors. The strength of this relationship can be seen from the value of OR and RR, among others factors of chicken breed (OR = 3.07; RR = 1.34), breeder's education (OR = 2.3; RR = 1.29), type of livestock business (OR = 7.5; RR = 1.43), type of animal feed (OR = 1.91; RR = 1.2), veterinary support for livestock raising management (OR = 3.09; RR = 1.44). The reference variables are whether the antibiotics are administered by non-veterinarians (OR = 2.35) or by the TS (OR = 7.92), and whether there is an antibiotic administration program (OR = 3.16; RR = 1.47). The overseeing function of farm maintenance, management, and implementation of antimicrobial administration in commercial chicken farms needs to be improved, to increase breeders' awareness of the careful usage of antibiotics and controlling the incidence of antibiotic resistance.

Identification of Ectoparasites and Gastrointestinal Tract Endoparasites in Stray Cats at Traditional Market of Bojonegoro City Karina Rachmawati; Setiawan Koesdarto; Iwan Sahrial Hamid; Dian Ayu Permatasari
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

ABSTRACT This study aims to determine the ectoparasites and endoparasites on gastrointestinal tract that infest stray cats in Bojonegoro traditional market. The samples in this study were ectoparasites and feces from 40 stray cats in Bojonegoro traditional market. The ectoparasite samples were examined using the permanent attachment method without staining, while the feces samples were examined using three methods, namely native, simple sedimentation and fulleborn floatation. A positive sample is considered if a parasite is found that matches the characteristics of a parasite in cats sourced from scientific references. The results of the examination of 40 stray cats found 27 positive ectoparasites, 23 positive worms and 6 positive protozoa. The types of ectoparasites found were Ctneocephalides felis (67.5%), Otodectes cynotis (15%) where age and gender did not affect the prevalence, while the endoparasites found were Toxocara cati (42.5%), Ancylostoma sp. (22.5%) and Isospora felis (15%), age has effect on the prevalence obtained and sex has no effect . Keywords : Stray cats, Ectoparasites, Endoparasites in gastrointestinal tract

Detection Of Parasitic Helminth In Gabus Fish (Channa Striata) Natural Catches At Baduk Fish Market Nganjuk Regency Anita Nur Aida Aida; Setiawan Koesdarto; Emy Koestanti Sabdoningrum; Dian Ayu Permatasari
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.44287

This study aimed to detect the existence and identify, also to determine the prevalence and degree of infection from parasitic helminth in gabus fish (Channa striata) natural catches at Baduk Fish Market, Nganjuk Regency. In 50 samples of gabus fish, parasitic helminths were examined on the body surface, gills, abdominal cavity, muscles, and digestive tract then identified. Parasitic helminths from digestive tract stained with Semichen Acetic Carmine method. Parasitic helminths that have been identified then calculate the prevalence and degree of infection. The result showed that the parasitic helminths found in gabus fish consist of the genera Pallisentis, Spinitectus, Dactylogyrus, and Gyrodactylus. Pallisentis sp. included in Phylum Acanthocephala, found to infect the intestines with prevalence of 74% and degree of infection of 5,62 parasite each fish. Spinitectus sp. included in Phylum Nematoda, found to infect the stomach and intestines with prevalence of 48% and degree of infection of 8 parasite each fish. Dactylogyrus sp. included in Class Monogenea, found to infect the gills with prevalence of 80% and degree of infection of 1,95 parasite each fish. Gyrodactylus sp. included in Class Monogenea, found to infect the body surface with prevalence of 42% and infection rate of 1,33 parasite each fish.

Hubungan teknik pemerahan dengan jumlah Escherichia coli pada susu segar dari peternakan sapi perah di KUD Kertajaya, Kabupaten Kediri, Jawa Timur Rahadyan, Raihan Alif; Tyasningsih, Wiwiek; Puspitasari, Yulianna; Permatasari, Dian Ayu; Widjiati, Widjiati
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.19-24

Background: The coliform bacteria group is a group of bacteria used as an indicator of food sanitation, one of which is in dairy products. Objective: This study aimed to determine the relationship of milking techniques with the number of Escherichia coli in raw milk compared to the bacterial contamination limit set by the National Standardization Agency. Methods: The design of this study was a descriptive study. Raw milk samples were taken from Lucky Farm and several traditional dairy farms in the KUD Kertajaya area, Kediri Regency, East Java. This study uses the most probable number (MPN) test. Results: The results showed 11 (73.3%) of raw milk samples using conventional milking techniques exceeding the maximum limit of E. coli bacterial contamination determined by the National Standardization Agency (< 3 MPN/mL). However, all 15 samples, or 100% of raw milk samples using machine milking techniques, were negative for E. coli and met the maximum bacterial contamination limit requirements. Conclusion: The milking techniques affect E. coli contamination limits in raw milk. The milk samples taken using machine milking techniques had better results with MPN values < 3 MPN/mL than those taken using conventional milking techniques.

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