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All Journal Jurnal Sain Veteriner BERITA BIOLOGI Aquacultura Indonesiana Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Berkala Penelitian Hayati Journal of Fisheries & Marine Jurnal Ilmiah Pertanian dan Peternakan

Hari Suprapto

Faculty Of Fisheries And Marine Universitas Airlangga

Author-ID : 3288403

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Pemberian Vaksin Formalin Killed Cell (FKC) Vibrio alginolitycus untuk Meningkatkan Survival Rate (SR), Titer Antibodi dan Fagositosis Leukosit pada Kerapu Cantang (Epinephelus sp.) setelah Uji Tantang Bakteri Vibrio alginolitycus [Administration of Vaccine Formalin Killed Cell (FKC) Vibrio alginolyticus to Increase Survival Rate (SR), Antibody Titre, and Phagocytic Leukocytes Cantang Grouper (Epinephelus sp.) Against Bacteria Vibrio alginolitycus] Hari suprapto; Sudarno Sudarno; Nurul Qomariyah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 9 No. 1 (2017): Jurnal Ilmiah Perikanan dan Kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v9i1.7625

AbstrakVaksinasi merupakan suatu upaya untuk menimbulkan ketahanan tubuh yang bersifat spesifik melalui pemberian vaksin. Vaksinasi digunakan untuk mencegah serangan penyakit infeksi serta efektif untuk mengontrol serangan penyakit ikan seperti bakteri V. alginolyticus. Pengamatan tingkat kelulushidupan (SR) dan pengukuran titer antibody merupakan pengukuran efektivitas vaksinasi, sedangkan tingkat kesehatan ikan dapat dilihat dari hematologi salah satunya adalah fagositosis leukosit. Tujuan dari penelitian ini Untuk mengetahui tingkat kelulushidupan, titer antibodi, dan indeks fagositosis leukosit benih ikan kerapu cantang yang divaksin dengan vaksin FKC bakteri Vibrio alginolyticus. Penelitian ini dilakukan di Balai Perikanan Budidaya Air Payau Situbondo, Jawa Timur. Penelitian ini menggunakan metode Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah kontrol negatif, kontrol positif, 0,0125 mg/5 g BB ikan, 0,25 mg/ 5 g BB ikan, 0,5 mg/ 5 BB ikan. Analisis hasil menggunakan ANOVA dan dilanjutkan Uji Jarak Berganda Duncan. Hasil penelitian menunjukkan bahwa perlakuan pemberian vaksinasi memberikan pengaruh terhadap tingkat kelulushidupan (SR), titer antibody, dan indeks fagositosis benih ikan kerapu cantang. Jumlah tingkat kelulushidupan ikan kerapu cantang yang divaksinasi terbaik yaitu 70,0%, titer antibodi yang divaksinasi terbaik yaitu 298,67, dan indeks fagositosis yang terbaik yaitu 52,67%. Hasil penelitian dengan pemberian vaksin formalin killed cell (FKC) Vibrio alginolitycus dapat memberikan proteksi pada ikan dan penggunaan dosis yang berbeda mampu mempengaruhi tingkat kelulushidupan (SR), titer antibodi, dan indeks fagositosis AbstractVaccination is an attempt to give-specific endurance through vaccine. Vaccination is used to prevent infectious diseases and effective to control fish diseases such as bacterial attack V. alginolyticus. Observations survival rate (SR) and measurement of antibody titre is a measure of the effectiveness of vaccination, while the level of health of the fish can be seen from the hematology one of which is phagocytic leukocytes. The purpose of this research was to determine the effect of the vaccine Formaldehyde Killed Cell (FKC) Vibrio alginolitycus to increase survival rate (SR), antibody titre, and phagocytic leucocytes cantang grouper (Epinephelus sp.) This research used a completely randomized design with five treatments and three replications. The treatment used is the negative control, the positive control, 0.0125 mg / 5 g BB fish, 0.25 mg / 5 g BB fish , 0.5 mg / 5 BB fish. Analysis of the results using ANOVA and Duncan's Multiple Range Test continued. The results showed that the treatment of vaccination impact the survival rate (SR), antibody titre, and phagocytosis index cantang grouper seed. Total survival rate, antibody titre, and the best phagocytosis index cantang grouper with consecutive values as follows70,0%, 298,67, and 52,67%. This research that used the vaccine formaldehyde killed cells (FKC) Vibrio alginolitycus can provide protection to the fish and the use of different doses capable of affecting the survival rate (SR), antibody titre, and the index of phagocytosis

Prevalensi dan Derajat Infestasi Ektoparasit pada Udang Vaname (Litopenaeus vannamei) di Tambak Intensif dan Tradisional di Kabupaten Gresik [ Prevalence and Degrees of Infestation Ectoparasite on White Shrimp (Litopenaeus vannamei) in Intensive and Extensive Cultivation System in Gresik] Gunanti Mahasri; Hari Suprapto; Abyan Farras
Jurnal Ilmiah Perikanan dan Kelautan Vol. 9 No. 2 (2017): Jurnal Ilmiah Perikanan dan Kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v9i2.7640

AbstrakUdang putih (Litopenaeus vannamei) adalah komoditas unggulan budidaya yang memiliki harga yang signifikan pada pasar di seluruh dunia. Sepenuhnya permintaan udang, perlu perhatian serius dalam pembenihan vaname, dengan bertujuan untuk mendapatkan panen maksimal dan dapat memenuhi kebutuhan pasar. Tujuan penelitian ini untuk menentukan prevalensi dan derajat infestasi, serta mengetahui perbedaan prevalensi dan derajat infestasi ektoparasit pada udang vaname sistem intensif dan tradisional. Metode yang digunakan dalam penelitian ini menggunakan metode survei, yaitu metode pengamatan sistematis, faktual, dan akurat tentang fakta dan sifat populasi atau pada wilayah tertentu. Prevalensi ektoparasit yang menyerang udang putih di budidaya intensif 57,5% dan budidaya tradisional 56,6% dan dalam kategori sering (sering kali) tingkat infestasi rata - rata yang menginfeksi vaname putih dalam budidaya intensif adalah 76,56 (berat), sedangkan di budidaya tradisional adalah 43,78 (sedang). Hasil analisis data menunjukkan bahwa tidak ada perbedaan antara prevalensi budidaya intensif dan tradisional (p> 0,05), terdapat perbedaan tingkat infestasi ektoparasit antara prevalensi budidaya intensif dan tradisional Abstract White shrimp (Litopenaeus vannamei) is a species of cultivation and have important value in the worldwide market.Tofully a demand of shrimp, need for serious treatment in prawn hatchery vaname, with the aim that gets the maximum harvest and can fulfill a need market. The aims of this research to determine the prevalence and degree of infestation, as well as know the difference prevalence and degree of infestation ectoparasite on shrimps vaname which are in intensive and extensive cultivation. The method used in this study using survey methods, that is methods to make observation systematical, factual, and accurate about the fact and the nature of the population or to a definite region. The prevalence of ectoparasite that infests white shrimp in intensive cultivation is 57,5% and in traditional cultivation is 56,6% and in category frequently (often times). The average degree of infestation that infects white vaname in intensive cultivation is 76,56 (heavy), while in the traditional cultivation is 43,78 (medium). The results analysis of the data show that there is no difference between the prevalence of intensive and traditional cultivation (p > 0,05), there was difference infestation degree of ectoparasite between the prevalence of intensive and traditional cultivation.

Isolasi dan Identifikasi Bakteri Kitinolitik yang terdapat pada Cangkang Lobster Air Tawar (Cherax Quadricarinatus) [Isolation and Identification of Chytinolitic Bacteria from The Crayfish (Cherax Quadricarinatus) Shell] Hari Suprapto; Sudarno Sudarno; Istikhara Mentari Tito
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 1 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i1.11186

AbstrakIndonesia memiliki potensi perikanan yang sangat tinggi, salah satunya adalah lobster. Ekspor lobster air tawar cenderung meningkat tiap tahun. Total ekspor hasil lobster budidaya mencapai 94.511 ton/tahun. Pangsa pasar lobster air tawar tidak hanya terbatas di dalam negeri saja tetapi juga ke luar negeri. Penelitian ini bertujuan untuk mengetahui adanya bakteri kitinolitik dan juga jenis-jenis bakteri kitinolitik yang terdapat pada cangkang lobster air tawar. Penelitian ini menggunakan Penelitian ini menggunakan metode observasi untuk mengetahui adanya bakteri kitinolitik yang ada pada cangkang lobster air tawar. Data yang digunakan dalam penelitian ini adalah secara deskriptif. Data yang diperoleh dianalisis secara deskriptif yaitu penyajian data tentang morfologi dan karakteristik dari bakteri kitinolitik yang diisolasi dari cangkang lobser air tawar (Cherax quadricarinatus) dan dibandingkan dengan morfologi dan karakteristik bakteri kitinolitik dengan literatur yang sesuai. Hasil penelitian ini adalah diperoleh bahwa bakteri yang diisolasi dari cangkang lobster air tawar (Cherax quadricarinatus) dapat tumbuh dan berkembang pada media uji. Kemudian hasil uji hidrolisis bakteri kitinolitik ditandai dengan adanya zona bening yang dihasilkan dari bakteri tersebut. Dari hasil pengujian tersebut didapatkan jenis – jenis bakteri yaitu Aeromonas sp., Bacillus sp. dan Pseudomonas sp. AbstractIndonesia have highest potential of fishery. One of them is Lobster. It has increasing slightly, approximately 94.511 ton/years. The aims this research to determine the presence of chitinolytic bacteria and identify its species in crayfish shells. This research was performed via observation method to determine the presence of chitinolytic bacteria, which are exist in crayfish (cherax quadricarinatus) shell. The data used in this research is descriptive. The data descriptively analyzed represented the morphology and characteristics of chitinolytic bacteria isolated from crayfish (Cherax quadricarinatus) and comparison the morphology and characteristics of chitinolytic bacteria with appropriate literature. These results obtained that the bacteria isolated from crayfish (Cherax quadricarinatus) shell can grow and develop in the test medium. Then, chitinolytic activity was signed by the formation of clear zone on the test medium. The results obtained several bacterium species including Aeromonas sp., Bacillus sp. and Pseudomonas sp.

Potensi Bacillus licheniformis dan Streptomyces olivaceoviridis sebagai Penghambat Pertumbuhan Jamur Saprolegnia sp. Penyebab Saprolegniasis pada Ikan secara In Vitro [Potential of Bacillus licheniformis and Streptomyces olivaceoviridis as Inhibiting The Growth of Fungus Saprolegnia sp, Cause Saprolegniasis on Fish by Using In Vitro] Oktantia Frenny Anggani; Rahayu Kusdarwati; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11196

Abstract Saprolegniasis is a fungal disease on fish caused by Saprolegnia sp, which is saprophyte, damaging healthy tissue and makes the immune system in fish deacreased. The uniquennes of Saprolegnia sp has the main components of the cell wall in the form of chitin that was instrumental in shaping the structure of the tip growth of fungal hyphae. Control of fungal pathogenic Saprolegnia sp can use chitinolytic microorganisms based on ability to produce chitinase for example using bacteria. This study aims to potential of Bacillus licheniformis and Streptomyces olivaceoviridis as inhibiting the growth of fungus Saprolegnia sp, cause saprolegniasis on fish by using in vitro. The research method is experimental with completely randomized design (CRD), which consists of four treatments with five replications. The experimental used is A (Saprolegnia sp and Bacillus licheniformis), B (Saprolegnia sp and Streptomyces olivaceoviridis), C negative control (Saprolegnia sp) and D positive control (Saprolegnia sp and Ketokonazol 2 %). The main parameters measured were observed inhibition zone on each treatment. Supporting parameters were observed is an observation of abnormal hpyphae structure after being induced by bacteria Bacillus licheniformis dan Streptomyces olivaceoviridis. Data were analyzed using analysis of variants (ANOVA) and to know the difference between treatments were determined by Tukey honestly significant difference (Tukey HSD) Test. The results showed that the potential of chitinolytic Bacillus licheniformis can provide a good barrier of 4,62 cm by 5,48 cm compared Streptomyces olivaceoviridis in inhibiting the growth Saprolegnia sp. Suggestions in this research is the need to further research on the value of chitinase Bacillus licheniformis and Streptomyces olivaceoviridis. Further research by using in vivo.

Deteksi Koi Herpesvirus (KHV) pada Ikan Nila (Oreochromis niloticus) yang Diinfeksi secara Buatan [ Detection of Koi Herpesvirus (KHV) in Nile Tilapia (Oreochromis niloticus) Infected by Artificially Infection ] Hari Suprapto; Suwarno Suwarno; Muhammad Sungging Pradana
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11230

Abstract Koi Herpesvirus (KHV) was formerly known as Cyprinid herpesvirus-3 (CyHV-3) and carp nephritis interstitial and necrosis gill virus (CNGV) is a virus that infects common carp and koi (Cyprinus carpio and C. carpio koi) in farmed and wild population. KHV cause disease at a temperature of 18-25 °C with mortality rate of 80-90 % in fry and adult fish. Currently KHV also detected in tilapia from the results of monitoring in the field. The presence of KHV in tilapia can occur as a result of maintenance in cages adjacent to the infected carp. KHV diagnostic method currently based on case definition and PCR (Polymerase Chain Reaction) detection. The basic concept of PCR is one DNA molecule is used to produce two copies, then four, then eight and so forth through multiplication by polymerase. PCR results sometimes indicated the presence of a faint band caused a low amount of virus, so it is necessary to investigate the presence of KHV DNA in tilapia using different doses of infection. This study aimed to determine the KHV infectivity in nile tilapia were infected by artificially infection and determine dose KHV infection that can infect nile tilapia. The study design used true experimental with with the presentation of descriptive data. Dose of viral infection are 1 ID50, 10 ID50, 100 ID50 and 1000 ID50. The results showed that no clinical symptoms of KHV infected in nile tilapia. The results of electrophoresis of PCR products showed that the mucus of nile tilapia were infected with a 1000 ID50 immersion dose showed thin bands. The same results are also shown in the gill of nile tilapia infected by gill spray at 1000 ID50 dose. Fish were infected by injection, KHV was not detected in mucus, gill, kidney and liver. The results above show nile tilapia cannot be infected by KHV on various treatment

Identifikasi Koi Herpesvirus pada Dosis yang Berbeda dengan Metode Imunohistokimia Streptavidin Biotin pada Ikan Nila (Oreochromis niloticus) [Identification of Koi Herpes Virus At Different Dose with Streptavidin Biotin Methods Immunohistochemistry on Tilapia (Oreochromis niloticus)] Hari Suprapto; Faricha Risma Nurani; Suwarno Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11277

Abstract KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations. Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus). This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000. The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms

Pengaruh Pemberian Sari Kecambah Kacang Hijau terhadap Kadar Malondialdehid pada Ikan Lele Dumbo (Clarias gariepinus) yang Terinfeksi Bakteri Aeromonas hydrophila [Effect of Mung Bean Sprouts Essences against Malondialdehyde Levels In African catfish (Clarias gariepinus) Infected by Bacteria Aeromonas hydrophila ] Hari Suprapto; Widy Ayunanti; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11302

Abstract African catfish (Clarias gariepinus) is a type of freshwater fish consumption with elongated body and smooth skin. Bacterial disease that often strikes African catfish and can cause death as well as a cause of primary infection is A.hydrophila. Bacterial infection may be one cause of the synthesis of lipid peroxidation which is an indication of the emergence of free radicals. And the end result of lipid peroxidation is malondialdehyde. Sprouts are the new little plants grow from seeds planted beans. Green bean seed germination will enrich vitamins including vitamin E, green beans or α-tocopherol. Vitamin E in maintaining the stability of the plasma membrane and protects the cell structure against damage caused by free radicals is by blocking the reaction initiation and propagation of lipid peroxidation reactions interrupt. Data analyze of this research was used Varian Analyze (ANAVA) and to found the lowest level was used Duncan Test. The average yield of malondialdehyde levels in the negative control treatment is 0.0386 ng / 100 mg mass, positive control (1 ml Aeromonas hydrophila 105 cfu per 5 liter) is 0.0962 ng / 100 mg mass, sprout extract treatment (0 , 1 ml per fish) is 0.05 ng / 100 mg mass, germination and pollen treatment Aeromonas hydrophila is 0.0626 ng / 100 mg mass. From these results indicate that the highest levels found in the positive control treatment while the lowest levels found in the negative control. These results also suggest that decreased levels of malondialdehyde using pollen germination is very significantly different with P < 0,01.

Predileksi dan Perubahan Patologi Anatomi pada Ikan Maskoki (Carassius auratus) akibat Infestasi Lernaea cyprinacea Di Kabupaten Tulungagung [Predilection and Anatomical Pathology Changes in Goldfish (Carassius auratus) Due to Lernaea Cyprinacea Infestation in Tulungagung] Kismiyati Kismiyati; Nadia Ayu Fadila Asshaufi; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11303

Abstract Goldfish (Carassius auratus) is one of the most popular freshwater fish, therefore they has a very great potential market. Increased effort in Goldfish (Carassius auratus) aquaculture industry, fish farmer also faced greater problems. One of it is disease caused by ectoparacites Lernaea cyprinacea. The predilection of Lernaea cyprinacea can be be directly visible, by the presence of parasites attached to the body surface, fins, gills, eyes and mouth of fish. Lernaea cyprinacea attack on fish can cause pathological change such as lesions and nodules on the skin and fins. This research was conducted in March-November 2013. Location of sampling conducted in Tulungagung East Java while Goldfish (Carassius auratus) pathological examination in the Laboratory of Faculty of Fisheries and Marine Airlangga University. The purpose of this study was to determine the predilection of Lernaea cyprinacea that mostly infested on Goldfish (Carassius auratus) and knowing the change of anatomical pathology in Goldfish (Carassius auratus) infested by Lernaea cyprinacea. The method used was a survey method with random sampling technique and change in anatomic pathology described by the scoring method. The result showed that the predilection of Lernaea cyprinacea mostly infested on Goldfish (Carassius auratus) on dorsal and caudal fin, anatomic pathology changes on Goldfish (Carassius auratus) that infested by Lernaea cyprinacea are abscess, haemorhage, laceration, and fins damaged

Pengaruh Ekstrak Rimpang Kencur (Kaempferia galanga L.) terhadap Tingkat Kesembuhan Benih Ikan Lele Dumbo (Clarias sp.) yang Terinfeksi Saprolegnia sp. [Effect of Rhizome Extract (Kaempferia kalanga L.) for the Cure Rate of Catfish (Clarias sp.) Infected by Saprolegnia sp. ] Hari Suprapto; Nadia Fierias Fathma Riza; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11304

Abstract Saprolegnia sp. is a fungus that causes the disease Saprolegniasis on eggs and fresh water fish such as catfish (Clarias sp.). Treatment of Saprolegniasis have done using chemical drugs such as malachite green, formalin, sodium chloride and asetic acid. However, the use of chemical drugs is bad for the environment and biota. The purpose of this study is to determined the antifungal activity from rhizome extract (Kaempferia galanga L.) for the cure rate of catfish fry (Clarias sp) infected by Saprolegnia sp. The research was conducted on June 2013 at Fisheries and Marine Faculty of Airlangga University. The method used in this study is experimental method using Kruskall Wallis followed by Two Sample Independent test design with 5 treatments and four repetitions. Results showed that the extract of Kaempferia galanga L. has antifungal activity to recovery infected catfish fry. The optimal concentration from this research is 64%. The existence of antifungal activity of Kaempferia galanga L. extracts to Saprolegnia sp. showed that Kaempferia galanga L. extract consists some compounds as fungistatic and fungicidal such as flavonoid, saponin, phenolic and essential oil compounds.

Uji Aktivitas Antifungi Perasan Daun Kemangi (Ocimum sanctum Linn.) terhadap Aspergilllus terreus secara In Vitro [Antifungal Activity Test of Basil Leaves Juice (Ocimum sanctum Linn.) against Aspergilllus terreus by in Vitro] Sudarno Sudarno; Tyfany Imanu Sabrina; Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11305

Abstract Aspergillus terreus is a fungus that causes aspergillosis disease. The infected fishes showed grey white patches over the body. Haemorrhagic ulceratic patches were observed on the gill and skin. The infections resulted in the death of the fishes. The use of chemicals to control fungal attack A. terreus can harm fish, the environment and humans who eat them. Treatment of fungal diseases that use a lot of chemicals that can harm the fish, the environment and humans who eat them. The use of medicinal plants is a safe way to inhibit and kill fungus growth as well as environmentally friendly. One of them uses the basil plant (Ocimum sanctum). The purpose of this study was to determine the minimum concentration of activity and basil leaf juice (O. sanctum Linn) as antifungal against A. terreus growth in vitro . The experiment was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University in July 2013. The method used in this research that the paper disc diffusion method and diffusion pitting. The analysis used in this study is descriptive statistica. The results of research using the juice of basil leaves (O. santum) of juice concentration of 50% (2,5 ml juice of basil leaves + 2,5 ml NaCl) to concentration 100% (5 ml juice of basil leaves) did not produce a clear zone around the paper discs and pitting, it is the same as the negative control. The positive control did not show fungus growing on Sabouraud Dextrose Agar medium (SDA).

Co-Authors . Suwarno A Kusyairi Abyan Farras Agustono - Ajik Azmijah Akhmad Syafroni Affandi Angga Bahtera Siswanto Boedi Setya Rahardja D. Handiyatno Dahlia Dahlia de Vries, Garry Cores Fabi Aisah Setiorini Faricha Risma Nurani Garry Cores de Vries Gunanti Mahasri I Made Satya Widnyana Istikhara Mentari Tito Kismiyati , Kusnoto Kusnoto Kusyairi Kusyairi Kusyairi, A Kusyairi, Kusyairi L. Sumartiwi Laksmi Sulmartiwi Madyowati, Sri Oetami Meidiana Salsabila Miftahul Jannah Mirni Lamid Muhajir Muhajir Muhammad Ghufron Muhammad Sungging Pradana N. Juni Triastuti Nadia Ayu Fadila Asshaufi Nadia Fierias Fathma Riza Nunik Diantiwi Nurul Qomariyah Oetami, Sri Oktantia Frenny Anggani Rahayu Kusdarwati Reny Lidhia Widi Samsisko Riris Ulfiana S. Prawesthirini Sari, Putri Desi Wulan Setiawati Sigit Sri Oetami Sri Oetami Madyowati Sudarno Sudarno Sudarno, Sudarno Sulmartiwi, L. Sumaryam Sumaryam Sumaryam Sumaryam Sumaryam, Sumaryam Titik Desi Sukmawati Tyfany Imanu Sabrina Widy Ayunanti Yenta Kusuma Wardhani Yudhistira, Ary Yulia Kartika

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