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All Journal Jurnal Sain Veteriner BERITA BIOLOGI Aquacultura Indonesiana Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Berkala Penelitian Hayati Journal of Fisheries & Marine Jurnal Ilmiah Pertanian dan Peternakan
Hari Suprapto
Faculty Of Fisheries And Marine Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : BERITA BIOLOGI

 1 
KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Madyowati, Sri Oetami; Sumaryam, Sumaryam; Kusyairi, A; Suprapto, Hari
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.538 KB) | DOI: 10.14203/beritabiologi.v11i1.1878

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.
KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN) Sumaryam, Sumaryam; Kusyairi, Kusyairi; Oetami, Sri; Suprapto, Hari; de Vries, Garry Cores
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.805 KB) | DOI: 10.14203/beritabiologi.v10i4.768

Abstract

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.
KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Sri Oetami Madyowati; Sumaryam Sumaryam; A Kusyairi; Hari Suprapto
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1878

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.
KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN) Sumaryam Sumaryam; Kusyairi Kusyairi; Sri Oetami; Hari Suprapto; Garry Cores de Vries
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.768

Abstract

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.
Co-Authors . Suwarno A Kusyairi Abyan Farras Agustono - Ajik Azmijah Akhmad Syafroni Affandi Angga Bahtera Siswanto Boedi Setya Rahardja D. Handiyatno Dahlia Dahlia de Vries, Garry Cores Fabi Aisah Setiorini Faricha Risma Nurani Garry Cores de Vries Gunanti Mahasri I Made Satya Widnyana Istikhara Mentari Tito Kismiyati , Kusnoto Kusnoto Kusyairi Kusyairi Kusyairi, A Kusyairi, Kusyairi L. Sumartiwi Laksmi Sulmartiwi Madyowati, Sri Oetami Meidiana Salsabila Miftahul Jannah Mirni Lamid Muhajir Muhajir Muhammad Ghufron Muhammad Sungging Pradana N. Juni Triastuti Nadia Ayu Fadila Asshaufi Nadia Fierias Fathma Riza Nunik Diantiwi Nurul Qomariyah Oetami, Sri Oktantia Frenny Anggani Rahayu Kusdarwati Reny Lidhia Widi Samsisko Riris Ulfiana S. Prawesthirini Sari, Putri Desi Wulan Setiawati Sigit Sri Oetami Sri Oetami Madyowati Sudarno Sudarno Sudarno, Sudarno Sulmartiwi, L. Sumaryam Sumaryam Sumaryam Sumaryam Sumaryam, Sumaryam Titik Desi Sukmawati Tyfany Imanu Sabrina Widy Ayunanti Yenta Kusuma Wardhani Yudhistira, Ary Yulia Kartika