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KEMAMPUAN BUBUK EKSTRAK DAUN CINCAU HIJAU (Premna oblongifolia Merr.) DALAM MENSTIMULASI PERTUMBUHAN Lactobacillus casei subsp. rhamnosus Dwi Putri Nurmalasari; Nyoman Semadi Antara; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 4 (2017): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Dietary fiber with excellent fermentability can be categorized as prebiotics, one of which was green grass jelly. The objective of this research was to know the concentration of green grass leaf extract powder to stimulate the growth of Lactic Acid Bacteria (Lactobacillus casei subsp. rhamnosus). In vitro experiments were performed in laboratory with a concentration treatment of green grass leaf powder and glucose as a control. The medium used was yeast-peptone medium added with 1% glucose, 0% green grass leaf extract powder, 0.1% green grass leaf extract powder, 0.2% green grass leaf extract powder, 0.3% green grass leaf extract powder, 0.4% green grass leaf extract powder, and 0.5% green grass leaf extract powder. Fermentation was carried out at 37oC with a fermentation time of 9 hours. The experimental results showed that the concentration of green grass leaf powder extract of 0.2% was the best, in performance to stimulating growth of LAB. This concentration of the extract was the critical concentration, since the process of medium solidification was occured at the extract concentration of more than 0.2%. Keywords: Lactobacillus casei subsp. rhamnosus, green grass jelly, prebiotic.
Karakteristik Bubuk Alginat dari Alga Coklat Sargassum sp. pada Perlakuan Waktu dan Suhu Maserasi Kadek Ngurah Ghandhi Danu Subagan; Lutfi Suhendra; Ni Made Wartini
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 1 (2020): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.614 KB) | DOI: 10.24843/JRMA.2020.v08.i01.p11

Abstract

Sargassum sp. is an example of a brown algae plant that produces alginates in the form of an organic polysaccharide polymer composed of two units of L-guluronic acid and D-mannuronic acid monomers. The polymer is hydrophilic, that is, alginate is a linear molecule with a high molecular weight, so it is easy to absorb water causing alginate compounds can be used as emulsifiers. This research aims to know the effect of maceration time and temperature on the characteristics of alginate powder from brown algae Sargassum sp. and determine the best maceration time and temperature to produce alginate powder from brown algae Sargassum sp. This research used a Factorial Randomized Block Design. The first factor is time (A) which consists of 3 levels, namely 1 hour, 2 hours and 3 hours. The second factor is temperature (B) which consists of 3 levels namely 50°C,60°C,70°C. Each treatment were grouped into 2 based on the time of implementation so that 18 units of the experiment were obtained. The variables observed were the yield, water content, ash content, and viscosity. The data obtained were then analyzed by variance analysis and continued using the HRD method (Honest Real Difference). The results showed that the treatment of maceration time and temperature had a very significant effect on the observed variables namely yield, moisture content, ash content, and viscosity. The results showed that the treatment of 3 hours with a temperature of 70±1°C was the best treatment to produce alginate powder from brown algae Sargassum sp. with yield characteristics 39.44±0.06 %, water content 9.86±0.04 %, ash content 31.75±0.02 %, and viscosity value 19.00±1.41 cPs. Keywords: maseration, time, temperature, alginate, Sargassum sp.
KARAKTERISTIK GULA CAIR YANG DIBUAT DARI PATI UBI GADUNG (Dioscorea hispida D.) DALAM VARIASI JENIS DAN KONSENTRASI ASAM Reni Okta Fitriani; Amna Hartiati; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 3 (2018): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.369 KB) | DOI: 10.24843/JRMA.2018.v06.i03.p03

Abstract

This study aims to know the effect of acid type and concentration on the characteristics of liquid sugar resulting from the hydrolysis of yam gadung (Dioscorea hispida D.) and the highest DE value from the hydrolysis of the yam gadung starch. This research uses Randomized Block Design of factorial pattern. The first factor is acid treatment consisting of 3 levels: hydrolysis of starch with HNO3, H2SO4, and HCl and second factor is acid concentration consisting of 3 levels: 3%, 4%, and 5% . Each treatment is grouped into 2 based on execution time. The variables observed in this study were total sugar reduction, reducing sugar content, Dextrose Equivalent (DE), clarity, and total dissolved solids. The best treatment determination was done by analysis on DE value of hydrolysis result of yam gadung starch. The treatment of acid type variation was very significant and the acid concentration had a significant effect on the value of DE of hydrolysed yam gadung starch. The best hydrolysis treatment of starchy yam starch is by using chloride acid variation at 3% concentration with Dextrose Equifalent (DE) value of 41,22%. Keywords : starch, yam gadung, hydrolysis, acid
Pengaruh Ukuran Partikel dan Lama Maserasi terhadap Karakteristik Ekstrak Virgin Coconut Oil Wortel (Daucus carota L.) sebagai Pewarna Alami Ni Komang Novy Trisna Ardyanti; Lutfi Suhendra; G.P. Ganda Puta
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 3 (2020): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.266 KB) | DOI: 10.24843/JRMA.2020.v08.i03.p11

Abstract

Carrots are vegetable plants that contain ?-carotene, carotenoids, and vitamin A which can be extracted with organic (non-polar) solvents. Virgin Coconut Oil (VCO) is a non-polar solute suitable for dissolving natural dyes and has a dielectric constant of 2.82 × 10-18, so that VCO can be used as a solvent in carotenoid extraction in carrots. The purpose of this study were to determine the effect of particle size and maceration duration on the characteristics of carrot VCO extract as a natural dye and obtain the best particle size and maceration time to obtain carrot VCO extract as a natural dye. This experiment was designed using a Randomized Block Design with two factors. The first factor is the particle size which consists of 3 levels, namely: P1 (40 mesh), P2 (60 mesh), and P3 (80 mesh). The second factor is maceration time which consists of 3 levels, namely: W1 (4 hours), W2 (6 hours), and W3 (8 hours). Data were analyzed by analysis of variance and continued with Tukey test. The results showed that particle size and maceration time a very significant effect on yield, total carotenoids, total ?-carotene levels, brightness level (L*), redness level (a*) and yellowish level (b*). Treatment particle size 80 mesh and maceration duration of 6 hours was the best treatment to extract carrot VCO with yield characteristics of 0.77 percent, total carotenoids 2425.9 (mg/L), total ?-carotene levels 123.39 (mg/L), brightness level (L *) 34.77, redness level (a *) 14.67, and yellowish level (b *) 25.84. Keywords : VCO, carrot, extraction, particle size, time maceration.
PENGARUH JENIS LEMAK DAN MINYAK NABATI PADA PROSES EKSTRAKSI SISTEM ENFLEURASI TERHADAP KARAKTERISTIK MINYAK ATSIRI BUNGA KAMBOJA CENDANA (Plumeria alba) Sasha Patrisia; Ni Made Wartini; lutfi suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 2 (2017): April
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

The extraction of enfleurage system is widely used to take volatile compounds in flowers. The purposes of this research were to know the influence of fat and vegetable oil in the extraction process of enfleurage system on sandalwood frangipani flower’s essential oil characteristic, and to determine the best combination of fat and vegetable oil that produced essential oil of sandalwood frangipani flower. This research was a factorial research which used Randomize Block Design with 2 factors. The first factor was the type of fat oil, which consist of butter and margarine. The second factor was the type of vegetable oil, which consist of coconut, palm, corn, and soy oil, so there were 8 combination of experiments. The experiments were done in two replications, resulting 16 units of experiment. The variables observed were rendement of essential oil, density, solubility in alcohol, and aroma preference. The objective data were analyzed by analysis of variance and then continuing by Duncan test. Meanwhile, aroma preference data were analyzed by using Friedman test. The best treatment were determined using effectiveness index test. The result showed that there was a significant influence of fat and vegetable oil on essential oil’s rendement and solubility in alcohol, but there was no significant effect on essential oil’s aroma preference. Combination treatment of butter and corn oil was the best treatment that produced essential oil of sandalwood frangipani flower with rendement, density, and solubility in alcohol was 4.13%, 0.83 g/ml, and 6.5 ml, respectively. The aroma preference of this product was in the range of ‘quite like’ to ‘like’.Keywords: enfleurage, sandalwood frangipani flower, fat oil, vegetable oil, Plumeria alba.
Pengaruh Jenis Pelarut dan Perbandingan Bahan dengan Pelarut terhadap Ekstrak Warna Kulit Pisang Kepok (Musa paradisiaca F.). I Gede Esti Widiantara; Ni Made Wartini; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 1 (2020): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.064 KB) | DOI: 10.24843/JRMA.2020.v08.i01.p01

Abstract

The aims of this study were to determine the effect of solvent type and their ratio between material and solvent on the color of banana peel extract.The experiment in this study using factorial randomized block design with two factor. The first factor was the solvent type which consists of three levels: n-hexsane, acetone, and ethanol. The second factor was the ratio of the solvent that consists of four levels: 1:6, 1:8, 1:10, and 1:12. The experiment were grouped into 2 group and its implementation in order to obtain 24 experimental units. Variables observed are yield, total carotenoid, and the best color intensity. The results of this study indicate that the comparison of ingredients with solvents significantly affected the total carotenoids and the level of redness (a *) while the yield, brightness (L *), and yellowish level (b *) had no effect. The interaction of solvent types significantly affected the total carotenoids and the level of redness (a *) while the interaction of solvent types on yield, brightness (L *), and yellowish level (b *) had no effect. The type of ethanol solvent with a ratio of material to of solvent (1: 6) is the best solvent that produces extracts of banana peels which produce dye extracts with a yield characteristic of 2,40 ± 0,11 %, carotenoids 0,67 ± 0,00 %, L* 2,40 ± 0,11, a* 56,58 ± 6,92, b* 1,66 ± 0,17. Keywords: Kepok banana peel, solvent type, ratio between material and solvent, extraction, natural coloring
Pengaruh Konsentrasi Etanol dan Waktu Ekstraksi menggunakan Metode Microwave Assisted Extraction (MAE) terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Kakao Diana Puspitaningtyas; G.P. Ganda Putra; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 3 (2021): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.042 KB) | DOI: 10.24843/JRMA.2021.v09.i03.p10

Abstract

This research was conducted to determine effect of ethanol concentration and extraction time on antioxidant activity of cocoa pod husk extract and to obtain ethanol concentration and extraction time using Microwave Assisted Extraction (MAE) that can produce extract with the highest antioxidant capacity. The experimental design used in this research was a factorial randomized block design, which consisted of two factors. The first factor was the concentration of ethanol which consisted of 3 level that namely ethanol 75%, 85% and 95%. The second factor was the time of extraction which consisted of 4 level namely 5, 10, 15, and 20 minutes. The data were analyzed by analysis of variance and continued with Tukey test. The results showed that the concentration and extraction time had a very significant effect on yield, total phenolic, total flavonoid and antioxidant capacity of cocoa pod husk extract. Interactions between treatments had a very significant effect on total phenolic and total flavonoid. Interaction on antioxidant capacity had a significant effect, but did not significantly effect to the yield of cocoa pod husk extract. The best treatment showed that extraction by ethanol 95% for 15 minutes using MAE which the characteristic yield was 11.85±0.84% total phenol was 149.36±0.92 mg GAE/g extract, total flavonoid was 91.03±2.31 mgQE/g extract, antioxidant capacity was 136.69±0.19 mg GAEAC/g extract. Keyword : Ethanol concentration, time extraction, antioxidants, cocoa pod husk.
Karakteristik Ekstrak Kulit Biji Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan pada Perlakuan Ukuran Partikel dan Waktu Maserasi Reren Rahmadhani; G.P. Ganda Putra; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 2 (2020): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.236 KB) | DOI: 10.24843/JRMA.2020.v08.i02.p09

Abstract

Cocoa beans husk is the highest waste of the cocoa fruit processing and has not been used optimally. The waste of cocoa beans husk contain polyphenol that can be used for antioxidants. Polyphenol on beans husk of cocoa can be extract using extraction method. This study aims were to determine the effect of the macerations and particle size on the extract beans husk of cocoa as a source of antioxidants and to determine the best of solvent concentration and particle size to produce extract of beans husk of cocoa a source of antioxidant. The study was the experimental research which designed by using randomized block desin (RBD) with two factors. The first factor was particle size that that consists of 40, 60 and 80 mesh. The second factor was maceration time, wich were done for 24, 36 and 48 hours. The data were analyzed by analysis of variance (ANOVA) and continued with the Tukey test. The results showed that maceration time and particle size had a very significant effect on yield, total phenolics and antioxidant capacity on the extract of beans husk of cocoa. Interactions between treatments had a very signifiacant effect on total phenolic and antioxidant capacity but significantly effect the yield on the extract of beans husk of cocoa. 80 mesh of particle size and 48 hours maceration time was thebest treatment for extracting beans husk of cocoa as a source of antioxidants with characteristic 14.28±0.7 percent of yields, 92.2±1.66 mg GAE/g of total phenolics, and 57.71±0.27 mg GAEAC/g of antioxidant capacity. Keywords : cocoa beans husk, extraction, particle size, antioxidants.
Pengaruh Konsentrasi Asam Stearat dan Suhu Gelatinisasi terhadap Karakteristik Komposit Bioplastik Tapioka dan Glukomanan Jhon Berry Finn Damanik; Bambang Admadi Harsujuwono; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 10 No 1 (2022): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.175 KB) | DOI: 10.24843/JRMA.2022.v10.i01.p05

Abstract

This study aims to determine the effect of stearic acid concentration and gelatinization temperature and the interaction of the two treatments on the characteristics of tapioca and glucomannan bioplastic composites, and determine the concentration of stearic acid and gelatinization temperature which can produce bioplastic composites with the best characteristics. This study used a randomized block design with two factors. The first factor is the concentration of stearic acid which consists of 0.2%, 0.4%, 0.6%, 0.8%. The second factor is the gelatinization temperature which consists of 4 temperatures, namely 70±1°C, 75±1°C, 80±1°C, 85±1°C. The variables observed in this study were tensile strength, elongation at break, elasticity, thickness expansion, water vapor transmission rate, biodegradation and functional group analysis. The data were analyzed for diversity and continued with Duncan's Multiple Comparison. The results showed that the concentration of stearic acid and gelatinization temperature had a very significant effect on tensile strength, thickness expansion, water vapor transmission rate and biodegradation. The interaction between treatments had a very significant effect on tensile strength, thickness expansion, water vapor transmission rate and biodegradation and significantly affected elongation at break and elasticity. The best tapioca and glucomannan bioplastic composites used a stearic acid concentration of 0.4% and a gelatinization temperature of 80±1°C which had the characteristics of a tensile strength of 23.86 MPa, elongation at break 8.70%, elasticity 274.40 MPa, thickness expansion 111.52%, water vapour transmission rate 1.05 g/m2.hour, biodegradation rate with 7 days old. Tapioca and glucomannan bioplastic composites contain functional groups of hydroxyl (O-H), alkyne (C?H), aldehyde (C=O), carboxylic acid (C-O), alkene (C-H) and hydroxyl hydrocarbon (CH2)n. Keywords : bioplastic composite, tapioca, glucomannan, stearic acid, gelatinization temperature
PENGARUH PERBANDINGAN BAHAN DENGAN PELARUT ASETON TERHADAP TOTAL FENOLIK, WARNA DAN KLOROFIL EKSTRAK Sargassum polycystum I Putu Fajardhiputra Hernes; Lutfi Suhendra; Luh Putu Wrasiati
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 2 (2018): April
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.92 KB) | DOI: 10.24843/JRMA.2018.v06.i02.p02

Abstract

Seaweed is one of the biological resources that its existence is very abundant in all marine waters in Indonesia. One of them is Sargassum polycystum which is a type of brown algae (Phaeophyceae). The purpose of this study was to determine the effect of comparison the material with the solvent to the color content, the total phenolic and chlorophyll of Sargassum polycystum extract, and to determine the comparative treatment of the material with best solvent to produce Sargassum polycystum extract. This experiment uses a simple Group Randomized Design (GRD) consisting 5 comparisons of materials with the acetone (w/v) ie (1:9), (1:11), (1:13), (1:15), (1:17). Furthermore, the treatment is repeated as much as 3 times based on implementation time, to obtained 15 units of experiments. The results showed the ratio of material with the solvent had a very significant effect (P<0.01) on the rendement parameters, brightness (L*), redness (a*), yellowishness (b *), total phenolic, chlorophyll a and total chlorophyll. The results had significant effect (P<0.05) shown on chlorophyll b parameters. The best treatment was obtained from the treatment with the highest value on several parameters tested ie, yield, total phenolic and chlorophyll content. Comparison of material with acetone solvent (1:15) (w/v) was the best treatment with yield of 2.27%, color intensity (L*) 5.08, (a*) -5.62, (b*) 54.00, total phenolic 0.95 mgGAE / 100g, chlorophyll a 279 ppm, chlorophyll b 134 ppm and total chlorophyll 310 ppm. Key words: Sargassum polycystum, compounds bioactive, comparison of material with solvent.
Co-Authors A. A. Dewi Anggreni Aldi Oktavian Aldo Taufan Reviandi Alfina Via Azizah Amirrulloh, Muchammad Rizqi Amna Hartiati ANAK AGUNG MADE DEWI ANGGRENI Anak Agung Made Dewi Anggreni Anissa Fitri Nur Aini Bambang Admadi Harjosuwono Bambang Admadi Harsojuwono Bambang Admadi Harsojuwono Bambang Admadi Harsujuwono Bambang Admadi Hasojuwono Chusnul Hidayat Chusnul Hidayat Chusnul Hidayat Dewi, Ni Made Raditya Shinta Diana Puspitaningtyas Dicki Cahya Putra Anggelo Dwi Putri Nurmalasari Eka Nur Diana Elfrida Ratnawati Emita Dwi Cahyana Erina Novandri Elsa Esra Palenta Sinaga Ezra Elkana Karo Sekali Fachriansyah Ismaimoon Fatahillah, Mufidah Riska G. P. Ganda Putra G.P. Ganda Puta G.P. Ganda Putra Gek Nanda Putri Dana Asih I Dewa Gde Mayun Permana I Gede Esti Widiantara I Gede Rusli Supariatna I Gusti Ayu Lani Triani I Gusti Ayu Sri Krsna Devi I Kadek Aditya Prasatya I Kadek Agus Nuada I Ketut Dio Prasetya I Ketut Satriawan I Komang Wiria Santiyoga I Made Dwipayana I Made Indra Pratista I Made Mas Oka Hendrawan I Made Suardana I Putu Fajardhiputra Hernes I Putu Hendra Adi Prasanta I Putu Lingsan Pratyaksa I W W Aryanika I Wayan Arnata I Wayan Mika Pratama I Wayan Tika Ida Ayu Bela Anggraeni Ida Ayu Kade Sintya Yulianti Dewi Ida Bagus Alit Arcana Ida Bagus Bas Baskara Ida Bagus Gede Brahmantara Ida Bagus Wayan Gunam Ika Martoquito Lumbanraja Irawaty Yolanda Hutajulu Irena Savitri Jhon Berry Finn Damanik Kadek Ngurah Ghandhi Danu Subagan Komang Gede Irwan Suparwan Komang Rumiarsa Luh Kurnia Dwi Indriyani Luh Putu Ayu Sumantining Luh Putu Wrasiati Luluk Nurmalasari Made Hary Sayoga Made Wahyu Nadaiswara Putra Mira Ardhaning Swari Misbach Baihaqi Zen Muhamad Erwin Efendi Mujahidah . Mujahidah Mujahidah Ni Kadek Eka Wati Ni Kadek Yeni Dwipayanti Ni Komang Novy Trisna Ardyanti Ni Luh Gede Dina Yunita Ni Luh Putu Ravi Cakswindryandani Ni Made Purindah Sari Ni Made Wartini Ni Made Wartini Ni Made Wartini Ni Nyoman Sri Yulianthi Ni Putu Noviantari Ni Putu Suwariani Ni Wayan Sukmayanti Novia Esterulina Purba NYOMAN SEMADI ANTARA Praycelia Marissa Miranda Pudji Hastuti Pudji Hastuti Putra, Anak Agung Gede Surya Pradana Putu Ayu Sucitawati Putu Julyantika Nica Dewi Putu Widya Sena Reni Okta Fitriani Reren Rahmadhani Rio Rambo Siallagan Riwina Bibina Br Sinulingga Riza Ibnu Fajar Rossa Ayu Sutardianie Rubbana Sunardi Sarah Chairunnisa Sasha Patrisia Shenni Maulina Sri Mulyani Sri Mulyani Sri Rahardjo Sri Raharjo Sumiyati - Supriyadi Supriyadi Tania Suciati Tia Larasati W. Angga Pranayasa Wiwik Sri Minarni Yohanes Setiyo Yohanes Wiliam Pagur