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Pengaruh Suhu dan Lama Ekstraksi dengan cara Maserasi terhadap Karakteristik Pewarna dari Ekstrak Alga Merah (Gracilaria sp.) Novia Esterulina Purba; Lutfi Suhendra; Ni Made Wartini
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 4 (2019): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.15 KB) | DOI: 10.24843/JRMA.2019.v07.i04.p01

Abstract

Red algae (Rhodophyta) contains vitamins, minerals, fiber, sodium, potassium and bioactive compounds in the form of secondary metabolites and the most important nutrients are pigments.This study aims to determine the effect of temperature and length of extraction by maceration on the characteristics of dyes from red algae extract (Gracilaria sp.) spand determine the best temperature and maceration time to produce the color extract of Gracilaria sp. This study used Factorial Randomized Block Design. The first factor is temperature which consists of 3 levels namely 30±1°C, 40±1°C, 50±1°C. The second factor is maceration length which consists of 3 levels, namely 24 hours, 30 hours and 36 hours. Each treatment was grouped into 2 based on the time of implementation so that 18 units of experiment were obtained. The variables observed were yield, phycoeritrin, color intensity and effectiveness test. The data obtained were then analyzed by variance analysis and test Tukey’s. The results showed that the treatment of temperature, maceration time and interactions between treatments had a very significant effect on yield, phycoerythrin, brightness (L*), redness level (a*) and yellowness level (b*). The temperature of 30±1°C and the maceration time of 30 hours is the best treatment to produce dyes from red algae extract (Gracilaria sp.) with the characteristics of yield 0.496 ± 0.001%, phycoerythrin 0.430 ± 0.006 mg/g, lightness (L*) 20.470 ± 0.141, redness level (a*) 21.790 ± 0.198 and yellowish level (b*) 12.205 ± 0.035. Keywords: temperature, length of maceration, color, phycoeritrin, Gracilaria sp.
PENGARUH UKURAN PARTIKEL BUBUK DAN KONSENTRASI PELARUT ASETON TERHADAP KARAKTERISTIK EKSTRAK WARNA Sargassum polycystum Ni Putu Noviantari; Lutfi Suhendra; Ni Made Wartini
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 3 (2017): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.935 KB)

Abstract

Sargassum polycystum is one type of brown seaweed from Indonesia that has potential as natural dye. The aims of this study were to determine the effect of particle size of powder and concentration of acetone solvent on characteristics color extract from Sargassum polycystum and to determine the best particle size of powder and concentration of acetone solvent to produce Sargassum polycystum color extract. The experiment in this study used Randomized Block Design (RBD) with two factors. The first factor is particle size of powder consisting of three levels: <40, 40, and 60 mesh. The second factor is the concentration of acetone solvent consisting of 4 levels: 65, 75, 85, and 95%. The results showed that the particle size of powder and concentration of acetone solvent affected significantly (P<0.01) on rendement, total carotenoid, total phenolic, brightness level (L*), redness level (a*) and yellowishness (b*). Interaction of both treatments affected significantly (P<0.01) on total carotenoid, total phenolic, redness level (a*) and yellowishness (b*), significant on rendemen (P<0.05), and did not effected (P>0.05) on brightness level (L*). The best treatment that produced color extract of Sargassum polycystum was obtained on particle size of powder from 60 mesh and concentration 95% of acetone solvent, which can produce 1.41% of rendement, 0.19% of total carotenoid, 16.02 mg GAE/ 100g of total phenolic, with brightness level (L*) 4.41, redness level (a*) -2.73 and yellowishness (b*) 38.66. Keywords : concentration of acetone solvent, the particle size of powder, Sargassum polycystum
PENGARUH VARIASI LARUTAN pH BUFFER TERHADAP KARAKTERISTIK EKSTRAK ALGA MERAH (Gracilaria sp.) SEBAGAI PEWARNA Esra Palenta Sinaga; Lutfi Suhendra; G. P. Ganda Putra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 3 (2019): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.443 KB) | DOI: 10.24843/JRMA.2019.v07.i03.p08

Abstract

Gracilaria sp. is one type of red algae containing phycoerytrin pigment. Phycoerytrin pigment can be used as a natural dye to replace synthesis dyes. Phycoerytrin is widely used as a natural dye for foods and cosmetics. The purpose of this research was 1) to determine the effect of variations pH buffer solution on the characteristics of red algae extract (Gracilaria sp.), 2) to determine the best pH buffer solution used to produce red algae extract (Gracilaria sp.). This study used a factorial Randomized Block Design with group variation of pH buffer. The treatment of materials with solvent variation of pH buffer consist of 8 level, namely: 3, 4, 5, 6, 7, 8, 9 and 10. Each treatment are grouped into two time-based implementation. The results showed that each treatment of pH buffer variation significantly affected the yield, the degree of phycoerytrin pigment, brightness (L *), redness (a *) and yellowish (b *). Buffer pH 7 produced the best extracts in red algae (Gracilaria sp.) with a yield of 0.6030%, pigment production 0.933 mg/g, brightness value 18.650 L *, redness value 24.470 a * and yellowish value 10.135 b *. Keywords: Gracilaria sp., phycoerytrin, pH Buffer, extract characteristic.
Pengaruh Perbandingan Minyak dan Surfaktan serta Suhu terhadap Karakteristik Sediaan Krim I Made Mas Oka Hendrawan; Lutfi Suhendra; G.P. Ganda Putra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 4 (2020): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.569 KB) | DOI: 10.24843/JRMA.2020.v08.i04.p04

Abstract

This study aims to determine the effect of oil and surfactant comparisons and the temperature to the resultant dosage characteristics and determine the oil and surfactant ratio and the appropriate temperature for the preparation of cream. This study uses the group random two-factor design. The first factor is the comparison of oil that is mixture of cocoa fat and virgin coconut oil with surfactants that is mixture of tween 80 and span 80 consisting of 3 levels, which are 85% ; 15%, 80% ; 20% and 85% ; 25%. The second factor is temperature consisting of 3 levels, which are 60 º C, 70 º C and 80 º C. Results of this study showed the treatment of oil comparison and surfactants were had an strongly effect to the homogeneity, adhesive time, spread ability, separation ratio and viscosity, but had no effect on the pH of cream preparations. Temperature treatment had an strongly effect on homogeneity, adhesive time, spread ability, separation ratio and viscosity, while only had an effect on the pH of a basic cream. Interaction between the treatment had an effect on the ratio of separation, pH as well as the spread of cream preparations, but no had effect on adhesive time and the viscosity of the preparation of cream. Oil comparison and surfactant treatment of 80%: 20% and temperature 70ºC is the best treatment resulting in a cream preparations with homogenity characteristics, adhesive time 7.14 Second, ratio of separator 0.48, spread ability of 6.83 cm, viscosity of 37000 cps and pH 6.05. Keywords: oil, surfactans, temperature, cocoa fats, virgin coconut oil, tween 80 and span 80
Kandungan Senyawa Bioaktif Teh Herbal Daun Kenikir (Cosmos caudatus Kunth.) pada Perlakuan Suhu Pengeringan dan Ukuran Partikel Luh Kurnia Dwi Indriyani; Luh Putu Wrasiati; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 1 (2021): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.803 KB) | DOI: 10.24843/JRMA.2021.v09.i01.p11

Abstract

Kenikir is a plant easily found in Indonesia. Kenikir leaves contain high levels of antioxidants, phenols, flavonoids, and vitamin C that kenikir leaves can be used as tea. This study aims to determine the effect of drying temperature and particle size on the content of bioactive compound in kenikir herbal tea and to determine the right drying temperature and particle size in order to produce the best characteristics of kenikir herbal tea. This experiment used a group factorial randomized design with two factors. The first factor was drying temperature which consisted of 50 ± 2 °C, 60 ± 2 °C, and 70 ± 2 °C. The second factor was the particle size consisted of 40, 60, and 80 mesh. The variables observed in this study were total phenols, total flavonoids, vitamin C and antioxidants. The data obtained were analyzed by analysis of variation (ANOVA) and analysis with real difference test (tukey) using Minitab 16 software. The results showed that the interaction between drying temperature and particle size had a very significant effect on total phenolics, total flavonoids, total vitamin C and antioxidant activity of kenikir herbal tea. The best treatment to produce kenikir leaf herbal tea was at a drying temperature of 70 ° C and a particle size of 80 mesh, with characteristics total phenol of 83.85 ± 0.12 mg GAE / g, total flavonoids of 7.25 ± 0.12 mg QAE / g, vitamin C 2.05 ± 027 mg / g and antioxidant activity (IC50) of 157.87 ± 0.08 ppm. Keywords : bioaktives compound ,drying temperature, kenikir herbal tea , size reduction
Resistensi Isolat Bakteri Asam Laktat dari Asinan Rebung Bambu Tabah (Gigantochloa nigrociliata Buse – Kurz) terhadap Antibiotik Irawaty Yolanda Hutajulu; Nyoman Semadi Antara; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 10 No 1 (2022): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.005 KB) | DOI: 10.24843/JRMA.2022.v10.i01.p11

Abstract

Probiotics are helpful bacteria that aid in the prevention and treatment of a variety of nutritional and health issues. Antibiotic overuse is a major contributor to the emergence of antibiotic-resistant pathogenic microorganisms. The goal of this study is to determine the level of antibiotic resistance of lactic acid bacteria isolates from pickled tabah bamboo shoots as a candidate for probiotic culture against the antibiotics amoxicillin, cefadroxil, and erythromycin, as well as the concentration level of antibiotic resistance. The diffusion method employed is paper disc diffusion. According to the research results, isolate PR.6.15.2 was resistant to erythromycin concentrations of 100 ppm (v/v), isolate PR.3.15.1 was resistant to concentrations of cefadroxil 100 ppm (v/v), erythromycin 100 ppm (v/v), and 200 ppm (v/v), with the diameter of the inhibition zone forming in a sequential order of 9.0 mm, 9.5 mm, 7.3 mm, and 8.8 mm. Keywords: bamboo shoots, tabah bamboo shoots, lactic acid bacteria, amoxicillin, erythromycin, cefadroxil
Karakteristik Ekstrak Kulit Buah Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan pada Perlakuan Perbandingan Bahan dengan Pelarut dan Waktu Maserasi Ni Kadek Yeni Dwipayanti; G.P. Ganda Putra; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 2 (2020): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.349 KB) | DOI: 10.24843/JRMA.2020.v08.i02.p06

Abstract

Cocoa pod husk is a waste of cocoa processing that is large enough and has not been used optimally. Cocoa pod husk waste can be used more optimally by extracting polyphenols compounds and used as natural antioxidants. This research aims to determine the effect of comparison of materials with solvents and maceration time against cocoa pod husk extracts as a source of antioxidants as well as to determine the material comparison with the solvent and the best maceration time for produce cocoa pod husk extract as a source of antioxidants. The study uses a random design of a group of two factors. The first factor is the comparison of materials with solvents consisting of 1:10, 1:15 and 1:20. The second factor is the maceration time consisting of 24, 36 and 48 hours. The data was analyzed its diversity and continued with the Tukey test. The results of the study showed a comparison of material with solvent and maceration time was very influential to the yield, total phenolic and antioxidant capacity of cocoa pod husk extract. The interaction between treatment is very influential on the total phenolic and antioxidant capacity but has no effect on the cocoa pod husk extract yield. The best treatment to produce cocoa pod husk extract as a source of antioxidant is the comparison of materials with solvent 1:20 and maceration time for 48 hours with a yield characteristic 3.10 ± 0.36 percent, total phenolic at 146.67 ± 5.14 mg GAE/g and antioxidant capacity 97.00 ± 2.22 mg GAEAC/g. Keywords: cocoa pod husk extract, comparison of materials with solvents, maceration time, antioxidant.
Pengaruh Jenis Pelarut dan Ukuran Partikel Bahan terhadap Karakteristik Ekstrak Daun Bidara (Ziziphus mauritiana L.) sebagai Sumber Saponin Ika Martoquito Lumbanraja; Ni Made Wartini; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 4 (2019): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.35 KB) | DOI: 10.24843/JRMA.2019.v07.i04.p06

Abstract

Bidara (Ziziphus mauritiana L.) is widely grown in the tropics. Bidara is rich in benefits because it has content of saponins contained in the leaves. Saponins compounds derived plants can be used as soap foaming agents. Saponins can be applied in the manufacture of soap because of its nature as a natural surfactant. The purpose of this study were to determine the influence of solvent type and particle size of materials againts on the characteristics bidara leaf extract as a source of saponins, get the best solvent type and particle size to produce bidara leaf extract as a source of saponins. This study used a Randomized Block Design with treatment of solvent type and particle size of materials. Treatment of solvent type consists of 3 levels, namely: methanol, ethanol and acetone. The particle size of materials treatment also consists of 3 levels, namely: 40 mesh, 60 mesh and 80 mesh. Each treatment are grouped into two-based implementation. The results showed that treatment of solvent type and particle size of materials very significant effect on yield, coarse extract saponins content and foam height leaves bidara as a source of saponins. Interaction between treatments very significant effect on yield and foam height. However, significant effect on coarse extract saponins content bidara leaves. The methanol solvent type treatment with particle size of material 80 mesh is the best treatment to produce bidara leaf extract as a source of saponins with value yield of 17.91±0.73 %, 20.40 ± 0.79% coarse extract saponins content and foam height of 8.27 ± 0.01 mm. Keywords : Ziziphus mauritina L., saponins, extraction, solvent type, the particle size of material.
KANDUNGAN SENYAWA FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK TEH HIJAU PADA PERLAKUAN SUHU AWAL DAN LAMA PENYEDUHAN Riza Ibnu Fajar; Luh Putu Wrasiati; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 3 (2018): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.438 KB) | DOI: 10.24843/JRMA.2018.v06.i03.p02

Abstract

Tea is a kind of plantations which has antioxidant compound and beneficial for health. Based on processing, there 3 kinds of tea namely black tea, green tea, and oolong tea. The aims of this study is to find out the temperature influence and time of brewing toward the flavonoid and antioxidant activity of green tea. This research was conducted to determine temperature and time brewing effect on flavonoid content and antioxidant activity of green tea. The experiment in this study used Randomized Block Design (RBD) with two factors. The first factor was temperature of brewing that consists of temperature 75oC, 85oC, and 95oC. The second factor was the time of brewing namely 5, 10, and 15 minutes. The treatment was repeated twice to obtain 18 units of the experiment. Data were analyzed with analysis of variance, followed by Duncan test. The results showed that the best treatment of green tea extract temperature of 95oC and extraction time 15 minutes resulted in yield 26.2±0,50%, total flavonoids 252.3±1,71 mg QE /g extract and IC50 value was 173.5±1,34 ?g/ml. Keywords : Green tea, brewing, flavonoid, antioxidant activity
Karakteristik Ekstrak Kulit Buah Kakao (Theobroma cacao L.) sebagai Sumber Antioksidan pada Perlakuan Konsentrasi Pelarut dan Ukuran Partikel Praycelia Marissa Miranda; G.P. Ganda Putra; Lutfi Suhendra
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 1 (2020): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.878 KB) | DOI: 10.24843/JRMA.2020.v08.i01.p04

Abstract

Cocoa pod husk is the highest waste of the cocoa fruit processing and has not been used optimally.The waste pod husk of cocoa contain polyphenol that can be used for antioxidants. Polyphenol on pod husk of cocoa can be extract using extraction method. This study aims were to determine the effect of the solvent concentration and particle size on the extract of pod husk of cocoa as a source of antioxidants and to determine the best of solvent concentration and particle size to produce extract of pod husk of cocoa as a source of antioxidant. The study was the experimental research which designed by using randomized block desin (RBD) with two factors. The first factor was solvent concentration that consists of 70, 80 and 90 percent of ethanol.The second factor wasparticle size that that consists of 40, 60 and 80 mesh. The data were analyzed by analysis of variance (ANOVA) and continued with the Tukey test. The results showed that the solvent concentration and particle size had very significant effect while the interaction did not affect significantly of the yield, total phenolics and antioxidant capacity of the extract of pod husk of cocoa. 90 percent of ethhanol concentration and 80 mesh of particle size was the best treatment for extracting pod husk of cocoa as a source of antioxidants with characteristic 10.78±0.61 percent of yields, 110.65±0.80 mg GAE/g of total phenolics and 55.08±0.78 mg GAEAC/g of antioxidant capacity. Keywords : pod husk cocoa, solvent concentration, particle size, antioxidants
Co-Authors A. A. Dewi Anggreni Aldi Oktavian Aldo Taufan Reviandi Alfina Via Azizah Amirrulloh, Muchammad Rizqi Amna Hartiati ANAK AGUNG MADE DEWI ANGGRENI Anak Agung Made Dewi Anggreni Anissa Fitri Nur Aini Bambang Admadi Harjosuwono Bambang Admadi Harsojuwono Bambang Admadi Harsojuwono Bambang Admadi Harsujuwono Bambang Admadi Hasojuwono Chusnul Hidayat Chusnul Hidayat Chusnul Hidayat Dewi, Ni Made Raditya Shinta Diana Puspitaningtyas Dicki Cahya Putra Anggelo Dwi Putri Nurmalasari Eka Nur Diana Elfrida Ratnawati Emita Dwi Cahyana Erina Novandri Elsa Esra Palenta Sinaga Ezra Elkana Karo Sekali Fachriansyah Ismaimoon Fatahillah, Mufidah Riska G. P. Ganda Putra G.P. Ganda Puta G.P. Ganda Putra Gek Nanda Putri Dana Asih I Dewa Gde Mayun Permana I Gede Esti Widiantara I Gede Rusli Supariatna I Gusti Ayu Lani Triani I Gusti Ayu Sri Krsna Devi I Kadek Aditya Prasatya I Kadek Agus Nuada I Ketut Dio Prasetya I Ketut Satriawan I Komang Wiria Santiyoga I Made Dwipayana I Made Indra Pratista I Made Mas Oka Hendrawan I Made Suardana I Putu Fajardhiputra Hernes I Putu Hendra Adi Prasanta I Putu Lingsan Pratyaksa I W W Aryanika I Wayan Arnata I Wayan Mika Pratama I Wayan Tika Ida Ayu Bela Anggraeni Ida Ayu Kade Sintya Yulianti Dewi Ida Bagus Alit Arcana Ida Bagus Bas Baskara Ida Bagus Gede Brahmantara Ida Bagus Wayan Gunam Ika Martoquito Lumbanraja Irawaty Yolanda Hutajulu Irena Savitri Jhon Berry Finn Damanik Kadek Ngurah Ghandhi Danu Subagan Komang Gede Irwan Suparwan Komang Rumiarsa Luh Kurnia Dwi Indriyani Luh Putu Ayu Sumantining Luh Putu Wrasiati Luluk Nurmalasari Made Hary Sayoga Made Wahyu Nadaiswara Putra Mira Ardhaning Swari Misbach Baihaqi Zen Muhamad Erwin Efendi Mujahidah . Mujahidah Mujahidah Ni Kadek Eka Wati Ni Kadek Yeni Dwipayanti Ni Komang Novy Trisna Ardyanti Ni Luh Gede Dina Yunita Ni Luh Putu Ravi Cakswindryandani Ni Made Purindah Sari Ni Made Wartini Ni Made Wartini Ni Made Wartini Ni Nyoman Sri Yulianthi Ni Putu Noviantari Ni Putu Suwariani Ni Wayan Sukmayanti Novia Esterulina Purba NYOMAN SEMADI ANTARA Praycelia Marissa Miranda Pudji Hastuti Pudji Hastuti Putra, Anak Agung Gede Surya Pradana Putu Ayu Sucitawati Putu Julyantika Nica Dewi Putu Widya Sena Reni Okta Fitriani Reren Rahmadhani Rio Rambo Siallagan Riwina Bibina Br Sinulingga Riza Ibnu Fajar Rossa Ayu Sutardianie Rubbana Sunardi Sarah Chairunnisa Sasha Patrisia Shenni Maulina Sri Mulyani Sri Mulyani Sri Rahardjo Sri Raharjo Sumiyati - Supriyadi Supriyadi Tania Suciati Tia Larasati W. Angga Pranayasa Wiwik Sri Minarni Yohanes Setiyo Yohanes Wiliam Pagur