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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Sain Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Agrovigor: Jurnal Agroekoteknologi Jurnal Kelautan : Indonesian Journal of Marine Science and Technology REKAYASA Jurnal Kelautan Tropis Jurnal Sumberdaya Akuatik Indopasifik Samakia: Jurnal Ilmu Perikanan Jurnal Kelautan Nasional Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Jurnal Airaha Journal of Fisheries & Marine
Wahyu Andy Nugraha
Program Studi Ilmu Kelautan, Universitas Trunojoyo Madura
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Library & Information Science Physics Social Sciences Veterinary Other

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Early Detection of Contamination Vibrio parahaemolyticus and Escherichia coli in Fisheries Product Using Multiplex Polymerase Chain Reaction Nur Hasanah; Putu Eka Sudaryatma; Imanuddin Razaq; Ni Nyoman Eriawati; Wahyu Andy Nugraha; Hidayati Kumalasari; Ni Putu Arya Shintya Anggraeni; Ida Ayu Mirah Meliana Dewi
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73314

Abstract

The fisheries sector provided a significant contribution to the Indonesian economy by increasing export activities in every year. The exported fisheries product are categorized of live fish, frozen fish, preservation products from various types of fish, crustaceans, and molluscs. The contamination of pathogenic bacteria Vibrio parahaemolyticus and Escherichia coli causing healthy problems originating from the fishery sector (sea-food borne disease). These two bacteria contaminated fisheries product is due to mishandling and storaging in the processing, which causes acute diarrhea, gastrointestinal infections and fever. The multiplex polymerase chain reaction (mPCR) method was developed to increase the efficiency of time, effort and accuracy of the bacterial contamination testing process. The mPCR method begins with the optimization of the two bacterial gene targets, sensitivity test, specificity test and then applied to samples of fishery products. The mPCR method is carried out in two mechanisms, namely “one-run” conducted from bacterial colonies isolated on agar media and “one-tube” which is applied directly from fishery products. The results of the development of the mPCR method on V. parahaemolyticus and E. coli resulted in sensitivity at concentrations of DNA 5.6 pg/ml and DNA 5.5 pg/ml, respectively. One-tube mPCR application obtained 7 positive colonies of V. parahaemolyticus and 38 positive colonies of E. coli. Meanwhile, one-tube mPCR which was applied directly from shrimp samples could identify the two bacteria.
Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction Hidayati Kumalasari; Putu Eka Sudaryatma; Artanti Tri Lestari; Wahyu Nurlita; Wahyu Andy Nugraha; Nur Hasanah; Ida Ayu Mirah Meliana Dewi; Ni Putu Arya Shintya Anggraeni
Jurnal Sain Veteriner Vol 40, No 2 (2022): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73599

Abstract

Indonesia become a maritime country with large of ocean area and beach line with marine species diversity reaches 37% of the world’s fish. This makes Indonesia have aquaculture potential that supported by an appropriate climate. However, the challenge of viral diseases caused by Infectious Spleen and Kidney Necrosis Virus (ISKNV) and Viral Nervous Necrosis (VNN) would hamper marine aquaculture and causing huge economic losses. Therefore, it is necessary to carry out early detection methods that are efficient, fast, precise and accurate to identifying these viral disease. Here we developed a multiplex-PCR (mPCR) a method that can detect simultaneously of the ISKNV and VNN. The results of this method showed high sensitivity and specificity by using MCP and CP gene target primers to detect ISKNV and VNN, respectively. From a total of 353 samples of seawater fish examined by mPCR, positive results following of single infection of ISKNV and VNN were 14 and 2, respectively. We also found that 18 grouper fish was co-infection with these viruses. From these results, it shows that the mPCR method developed has efficiency, faster and high accuracy. This founding was possible to be applied in laboratory testing or early detection system in the field for ISKNV and VNN. 
Seagrass Ecological Quality Index Pada Lokasi Yang Berbeda di Madura Nisa Riska Alif; Insafitri Insafitri; Wahyu Andy Nugraha
Juvenil Vol 3, No 4 (2022)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v3i4.18172

Abstract

ABSTRAKPenelitian dilakukan dilakukan di perairan Pulau Pagerungan Besar, Kecamatan Sapeken, Sumenep dan di perairan Desa Prancak, Kecamatan Sepuluh, Bangkalan. Tujuan penelitian untuk mengetahui nilai SEQI dan tutupan lamun di perairan Pulau Pagerungan Besar, Kecamatan Sapeken, Sumenep dan di perairan Desa Prancak, Kecamatan Sepuluh, Bangkalan. Metode yang digunakan dalam penelitian ini adalah transek garis pada 3 titik dan menggunakan transek kuadrat nerukuran 50 x 50 cm pada 2 lokasi yang berbeda. Parameter ketahanan yang digunakan kekayaan spesies lamun, tutupan lamun, tutupan makroalga, tutupan epifit, dan transparansi air. Pada penelitian ini ditemukan 4 jenis lamun yaitu Thalassia hemprichii, Enhalus acoroides, Cymodocea serrulata, dan Cymodocea rotundata dan rata-rata persentase tutupan lamun memperoleh 50% pada perairan Kecamatan Sapeken dan 22% pada kecamatan Sepuluh. Sedangkan hasil dari Seagrass Ecological Quality Indeks (SEQI) memperoleh 0.74 pada perairan Kecamatan Sapeken dan masuk dalam kategori baik dan pada perairan Kecamatan Sepuluh memperoleh hasil 0.62 masuk dalam kategori sedang.Kata kunci: SEQI, penutupan lamun, Sapeken, SepuluhABSTRACTThe research was conducted in the waters of Pagerungan Besar Island, Sapeken District, Sumenep and in the waters of Prancak Village, Sepuluh District, Bangkalan. The purpose of the study was to determine the value of SEQI and seagrass cover in the waters of Pagerungan Besar Island, Sapeken District, Sumenep and in the waters of Prancak Village, Sepuluh District, Bangkalan. The method used in this study is a line transect at 3 points and uses a ner squared transect measuring 50x50 cm at 2 different locations. The resistance parameters used are rich in seagrass species, seagrass cover, macroalgae cover, epiphytic cover, and water transparency. In this study, 4 types of seagrasses were found, namely Thalassia hemprichii, Enhalus acoroides, Cymodocea serrulata, and Cymodocea rotundata and the average percentage of seagrass cover obtained 50% in the waters of Sapeken District and 22% in Subdistrict Sepuluh. Meanwhile, the results of the Seagrass Ecological Quality Index (SEQI) obtained 0.74 in the waters of Sapeken District and were included in the good category and in the waters of District Ten obtained a result of 0.62 in the medium category.Keywords: SEQI, seagrass closure, Sapeken, Sepuluh
Pengaruh Salinitas yang Berbeda Terhadap Pertumbuhan Karang Lunak Cladiella Sp. Matius Gerry Nugroho; Muhammad Zainuri; Insafitri Insafitri; Wahyu Andy Nugraha
Jurnal Kelautan Nasional Vol 18, No 1 (2023): APRIL
Publisher : Pusat Riset Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkn.v18i1.9903

Abstract

Karang lunak mempunyai tekstur kerangka yang lunak berupa duri-duri kecil dari kalsium karbonat yang ada dalam jaringan tubuhnya. Pada umumnya karang lunak melekat pada substrat yang keras di dasar perairan. Penelitian ini bertujuan untuk mengetahui tingkat kelangsungan hidup, laju pertumbuhan relatif dan mutlak, dan perbandingan laju pertumbuhan karang lunak pada salinitas yang berbeda. Salinitas merupakan kadar garam terlarut pada air laut. Salinitas berperan penting untuk mendukung kehidupan biota laut termasuk karang lunak. Penelitian dilakukan di Laboratorium Ilmu Kelautan dari tgl 27 Januari 2019 sampai 09 Mei 2019 dengan cara mengukur panjang dan lebar fragmen karang hasil transplantasi yang di tempelkan di setiap akuarium dengan salinitas yang berbeda. Tingkat kelangsungan hidup yang didapatkan pada akuarium dengan salinitas 28, 31, dan 34 yaitu 100 % sedangkan untuk akuarium dengn salinitas 36 yaitu 40% karang lunak dapat hidup sampai akhir penelitian. Laju pertumbuhan karang lunak relatif bervariasi karena pengaruh lingkungan dan kualitas air. Sedangkan laju pertumbuhan mutlak panjang selama 3 bulan mendapatkan nilai yaitu Akuarium Salinitas 28 sebesar 3,221 cm/minggu dan 1,382 cm/minggu; Akuarium salinitas 31 sebesar 3,285 cm/minggu dan 1,433 cm/minggu; Akuarium salinitas 34 sebesar 1,753 cm/minggu dan 0,713 cm/minggu; dan Akuarium salinitas 36 sebesar 0,359 cm/minggu dan 0,224 cm/minggu. Ada perbedaan yang nyata pada laju pertumbuhan karang lunak Cladiella sp. pada salinitas yang berbeda..  Kata Kunci: Karang Lunak, Cladiella sp., Salinitas, Laju Pertumbuhan, Pertumbuhan
Efektivitas Alat Tangkap Mini Purse Seine Menggunakan Sumber Cahaya Berbeda Terhadap Hasil Tangkap Ikan Kembung (Rastrelliger sp.) [Fishing Tool Effectivity of Mini Purse Seine by Using Different Light Source for Catching Fish of Puffer Fish (Rastrelliger sp.) ] Akhmad Farid; Wahyu Andy Nugraha; Ifa Nur Rosyidah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11620

Abstract

AbstractIn fish catching activity, the fishermen in Banyuanyar district use mini tools for catching fish (purse seine) and use light assist tools for an operation in the night day. The light, that is used, is kerosene pressure lantern and mercury lights or lamp that is used on water surface (surface lamp). This kind of light is used to collect "pelagic” fish that has positive phototaxis characteristic. Puffer fish's response towards different sourse of light, that's kerosene pressure lantern and mercury light is needed to be known. Therefore, we can know the source of light that is more effective in order to collect the fish. It is hoped that the productivity of puffer fish will be developed for the fishermen. During the research with ten times repeating, the total account of puffer fish's haul that is resulted with two different treatment  by using kerosene pressure lantern and mercury light is that 810 kg and 1.460 kg. The data of puffer fish's haul that is gotten after using MannWhitney test shows that the kerosene pressure lantern and mercury light not to really different, especially in the haul of puffer fish in significant standart (0,05) is 2,262, while in Mann-Whitney test account is 0,171. That means the fishermans in Banyuanyar can use kerosene pressure lantern and mercury light for catching fish as the light assist tools for an operation in the night day. 
The Correlation of Coral Reef Cover and Rugosity with Coral Reef Fish Density in East Java Waters Wahyu Andy Nugraha; Faizun Mubarak; Eko Husaini; Hasyim Evendi
Jurnal Ilmiah Perikanan dan Kelautan Vol. 12 No. 1 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v12i1.14356

Abstract

HighlightsCoral cover, rugosity, and reef fish abundance was determinedThe relationship between coral cover and rugosity and fish abundance was analyzed.Coral cover in East Java were categorized as medium – good.The rugosity index and the fish abundance were highest in Bangsring Waters.There are no relationships between coral cover and fish abundance, but there is relationship between rugosity and fish abundance.AbstractA coral reef is one of the most complex and specific ecosystems in a tropical area. It is identified by its high productivity and biodiversity. This research aims to discover the percentage of coral reef cover and rugosity, as well as the fish abundance in several locations in East Java, Indonesia. In addition, this research aims to investigate the correlation of coral reef cover and rugosity with the density of coral reef fish. This research was conducted in three locations, i.e., Bangsring Waters of Wongsorejo District, Banyuwangi Regency; Gili Noko Bawean Island, Gresik Regency; and Kramat Island, Gili Genting District, Sumenep Regency. This research employed the Line Intercept Transect (LIT) method to calculate the percentage of coral reef cover, the Chain Intercept Transect (CIT) method to determine the coral reef rugosity, and the Belt Transect method to estimate the fish abundance. From this research, it was revealed that the highest coral reef cover was in Bawean waters, while the highest rugosity was in Bangring waters. In addition, the highest coral reef fish density was in Bangsring waters. Meanwhile, the coral reef fish density did not correlate with the percentage of coral reef cover. In contrast, it correlated with the coral reef rugosity. 
Struktur Komunitas Dan Tutupan Terumbu Karang di Pulau Gili Labak dan Gili Genting, Sumenep, Indonesia Alamsyah, Febri; Nugraha, Wahyu Andy; Insafitri, Insafitri
Buletin Oseanografi Marina Vol 13, No 3 (2024): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v13i3.62113

Abstract

Pulau yang memiliki terumbu karang di kabupaten Sumenep adalah Pulau Gili Labak dan Pulau Gili Genting. Belum ada yang penelitian yang menganalisis struktur komunitas dan persentase tutupan terumbu karang dengan menggunakan metode UPT di Gili Labak dan Gili Genting. Penelitian ini dilaksanakan pada bulan Agustus 2023 menggunakan metode Underwater Photo Transect (UPT). Hasil penelitian menunjukkan Gili Labak mendapatkan persentase penutupan 73,25% dan Gili Genting 55,37% dikategorikan baik. Karang Acropora yang mendominasi di Gili Labak adalah jenis Acropora Digitate sebesar 23,87% dan non-Acropora yang mendominasi adalah Coral Foliose sebesar 6,8%. Karang Acropora yang mendominasi di Gili Genting adalah Acropora Branching sebesar 34,69% dan non-Acropora yang mendominasi adalah Coral Massive sebesar 7%. Gili Labak memiliki 10 genus yaitu Pavona sp., Pachyceris sp., Pacillopora sp., Acropora sp., Montipora sp., Porites sp., Stylophora sp., Favia sp., Oxypora sp. dan Fungia sp. Gili Genting memiliki 8 genus yaitu Seriatopora sp., Pacillopora sp., Acropora sp., Montipora sp., Porites sp., Stylophora sp., Pavia sp. dan Fungia sp. Indeks keanekaragaman di Gili Labak 1,51 dan Gili Genting 1,42 dikategorikan sedang. Indeks keseragaman di pulau Gili Labak 0,65 dan Gili Genting 0,68 dikategorikan tinggi. Indeks dominansi di Gili labak 0,34 dan Gili Genting 0,35 dikategorikan rendah.  The islands that have coral reefs in the Sumenep district are Gili Labak Island and Gili Genting Island. No research has yet analysed the community structure and percentage of coral reef cover using the UPT method in Gili Labak and Gili Genting. This research was conducted in August 2023 using the Underwater Photo Transect (UPT) method. The results showed that Gili Labak and Gili Genting have good condition of coral reef which high life coral coverage 73.25% 55.37% respectively. Acropora digitate are dominate coral life form in Gili Labak (23.87%) while foliose corals, non-acroporids, are abundant which has coverage 23.87%. The dominating Acropora coral in Gili Genting is Acropora Branching by 34.69% and the dominating non-Acropora is Coral Massive by 7%. Gili Labak has 10 genera namely Pavona sp., Pachyceris sp., Pacillopora sp., Acropora sp., Montipora sp., Porites sp., Stylophora sp., Favia sp., Oxypora sp. and Fungia sp. Gili Genting has 8 genera namely Seriatopora sp., Pacillopora sp., Acropora sp., Montipora sp., Porites sp., Stylophora sp., Pavia sp. and Fungia sp. The diversity index in Gili Labak 1.51 and Gili Genting 1.42 is categorised as medium. The uniformity index on the islands of Gili Labak 0.65 and Gili Genting 0.68 is categorised as high. The dominance index in Gili Labak 0.34 and Gili Genting 0.35 is categorised as low.
Co-Authors Agil Gusti Tsanianto Nugroho Akhmad Farid Alamsyah, Febri Alex Purnomo Aries Dwi Siswanto Aries Dwi Siswanto Aries Dwi Siswanto Artanti Tri Lestari David Lolodo Dewi Parawita Diah Putri Fitriani Dian Maharani Eka Nurahemma Ning Asih Eko Husaini Eko Murniyanto Faizun Mubarak Fajar Miftachul Huda Fitriyah, Atiqotul Halida Nuriya Hasyim Evendi Henik Novitasari Hidayati Kumalasari Hidayati Kumalasari Hidayatullah Hasana Ida Ayu Mirah Meliana Dewi Ida Ayu Mirah Meliana Dewi Ifa Nur Rosyidah Ihsan Aditya Imanuddin Razaq Insafitri, I Kurratul Ainy Lisana Ainun Shafa Ariyanti Makhfud Effendy Matius Gerry Nugroho Milda Prasanti Nesi Wahyu Listiani Ni Nyoman Eriawati Ni Putu Arya Shintya Anggraeni Ni Putu Arya Shintya Anggraeni Nisa Riska Alif Nisa Riska Alif Nur Hasanah Nur Hasanah Putu Eka Sudaryatma Putu Eka Sudaryatma Rivaldi Awalli Putra Pratama S Supatno Tirtana, Eko Alfin Wahyu A'idin Hidayat Wahyu Nurlita Yoga Ibnu Graha Zainul Hidayah Zainul Hidayah