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PENGARUH MODAL, TENAGA KERJA DAN TEKNOLOGI TERHADAP HASIL PRODUKSI SUSU KABUPATEN BOYOLALI Nugroho, Satya; Budianto, Muchamad Joko
JEJAK: Jurnal Ekonomi dan Kebijakan Vol 7, No 2 (2014): September 2014
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jejak.v7i2.3896

Currently, most of milk in Indonesia should be imported. It is about 78,89 %, and the rest, that is about 22,11% is from local production or local dairy farmers. However, there are some problems faced by the dairy farmers in Kecamatan Musuk. They are capital, workforces, and technology. This study aims at knowing the influence of capital, workforces, and technology to the milk production in Kecamatan Musuk. Ordinary Least Square (OLS) was applied for analizing the data. The results show that the regression coefficient values of each independent variable (the variables of capital, workforces, and technology) positively influence the production of milk. Based on the T-test (partial), the capital and workforces influence positively and significantly to the production. In addition, technology also has positive effect to the milk production, but it is not significant. Finally, based on F-test, the milk production is influenced by the capital, workforces and technology. It is 87%.Â Saat ini sebagian besar susu di Indonesia masih harus di impor (sekitar 78,89%), sedangkan 22,11%nya di pasok dari produksi susu domestik yang sebagian besar dihasilkan oleh peternak sapi perah rakyat. Namun ada beberapa permasalaham yang kerap dihadapi oleh peternak sapi perah di Kecamatan Musuk, baik dari segi permodalan, tenaga kerja maupun teknologi yang dipakai.Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh dari penggunaan faktor produksi modal, tenaga kerja dan teknologi terhadap hasil produksi susu sapi perah di Kecamatan Musuk. Alat analisis yang digunakan adalah analisis regresi berganda atau Ordinary Least Square (OLS).Hasil analisis diperoleh bahwa nilai koefisien regresi masing-masing variabel bebas yaitu variabel Modal, Tenaga Kerja, dan Teknologi berpengaruh secara positif terhadap produksi susu sapi perah. Dari hasil uji-t (parsial) modal dan tenaga kerja berpengaruh positif dan signifikan sedangkan teknologi berpengaruh positif namun tidak signifikan. Uji F menunjukkan produksi susu sapi perah dipengaruhi oleh modal, tenaga kerja dan teknologi sebesar 87%.

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains also increased significantly (~16-22%). After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress. Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.

PARAMETER GENETIK DAN SELEKSI SORGUM [SORGHUM BICOLOR (L.) MOENCH] POPULASI F4 HASIL SINGLE SEED DESCENT (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2878

The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential

Understanding Tsetse Fly (Glossina morsitans) Behavior through its Genome Marbun, Kristin Talia; Nugroho, Satya; Jo, Juandy
Medicinus Vol 12, No 2 (2023): February
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v10i3.7036

Glossina morsitans (G. morsitans), commonly known as tsetse fly, have caused public health concerns throughout the years. G. morsitans is the vector for Trypanosoma brucei (T. brucei), the parasite responsible for causing the deadly African sleeping disease (African trypanosomiasis). Researchers have searched for ways to contain this disease, but to little avail. Fortunately, new advances in sequencing methods have given researchers a new opportunity to win the war against the disease. The whole-genome sequence of G. morsitans provides essential data regarding involved genes that transmits T. brucei to humans. Information about those unique genes facilitates researchers to create new methods to prevent G. morsitans from becoming the vector of T. brucei, enabling the containment of this disease. With this, we review the unique genes in the G. morsitans genome, such as those that contribute to blood-feeding ability, establish a relationship with symbionts, and G. morsitans unique sensory genes, with an expectation that it would enhance our knowledge of G. morsitans as the vector for parasites causing African trypanosomiasis.

Tar spot disease of sorghum plants caused by Phyllachora sp. in Bogor, Gunung Kidul and West Lombok, Indonesia Astuti, Dwi; Wiyono, Suryo; Hidayat, Sri Hendrastuti; Trikoesoemaningtyas, Trikoesoemaningtyas; Nugroho, Satya
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12338-46

The sorghum (Sorghum bicolor) plant is highly tolerant and adapted to drought. It has the potential to be developed and planted on marginal land in Indonesia. Tar spot disease is one of the obstacles to sorghum cultivation. In Indonesia, there is no report yet about this disease, especially on sorghum. To achieve optimal disease management, information on disease incidence and severity levels, as well as pathogen tar spot disease identification, are required. The aims of this study were to morphologically identify the pathogen and determine the level of incidence and severity of tar spot in sorghum cultivation areas in Bogor Regency, Gunung Kidul Regency, and West Lombok Regency. The disease observations were carried out on 17 sorghum varieties from the three areas with no experimental design. The results of the observation of the tar spot disease incidence in 17 varieties of sorghum from Bogor, Gunung Kidul, and West Lombok were 100%. The level of tar spot disease severity varied from 32.4% in the Latu Keta sorghum variety to the highest of 87.9% in the Samurai sorghum variety. The macroscopic and microscopic observation results of tar spot on sorghum plants showed that the cause of the tar spot disease was the Phyllachora fungi. This is the first report of a tar spot on sorghum caused by Phyllachora sp. in Indonesia.

Understanding Tsetse Fly (Glossina morsitans) Behavior through its Genome Marbun, Kristin Talia; Nugroho, Satya; Jo, Juandy
Medicinus Vol. 12 No. 2 (2023): February
Publisher : Fakultas Kedokteran Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/med.v10i3.7036

Glossina morsitans (G. morsitans), commonly known as tsetse fly, have caused public health concerns throughout the years. G. morsitans is the vector for Trypanosoma brucei (T. brucei), the parasite responsible for causing the deadly African sleeping disease (African trypanosomiasis). Researchers have searched for ways to contain this disease, but to little avail. Fortunately, new advances in sequencing methods have given researchers a new opportunity to win the war against the disease. The whole-genome sequence of G. morsitans provides essential data regarding involved genes that transmits T. brucei to humans. Information about those unique genes facilitates researchers to create new methods to prevent G. morsitans from becoming the vector of T. brucei, enabling the containment of this disease. With this, we review the unique genes in the G. morsitans genome, such as those that contribute to blood-feeding ability, establish a relationship with symbionts, and G. morsitans unique sensory genes, with an expectation that it would enhance our knowledge of G. morsitans as the vector for parasites causing African trypanosomiasis.

Genome-Wide SNP Discovery and Population Structure Analysis of 244 Indonesian Local Rice Accessions Satrio, Rizky Dwi; Fendiyanto, Miftahul Huda; Nugroho, Satya; Hairmansis, Aris; Miftahudin
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.142-152

Single Nucleotide Polymorphism (SNP) markers are essential tools for the genetic management and breeding of rice, enabling precise characterization of genetic diversity. However, SNP datasets specifically developed for analyzing the population structure of Indonesian local rice remain limited. This study aimed to identify polymorphic SNP markers and analyze the lineage and population structure of 244 Indonesian local rice accessions. BAM files of these accessions were obtained from the 3K Rice Genome Project via the International Rice Informatics Consortium. High-quality SNPs were generated through variant calling and stringent filtering, followed by annotation and population analyses using IR64, DJ123, and Nipponbare as references. A total of 3,839,961 high-quality SNP loci were identified across the 12 rice chromosomes. Hierarchical clustering and principal coordinate analysis consistently grouped the accessions into three major clusters, corresponding to Indica, Japonica, and admixed groups. Population structure analysis confirmed two main subpopulations: Indica and Japonica, with Japonica further divided into Temperate and Tropical subgroups. Eleven accessions showed admixed ancestry. This study provides essential insights into the genetic diversity and population structure of Indonesian local rice, supporting genetic resource conservation, trait discovery, and breeding efforts.

Seleksi Genotipe Padi Mutan Insersi Toleran Cekaman Salinitas Berdasarkan Karakter Pertumbuhan dan Biokimia Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Eleven genotypes of insertional mutant Nipponbare rice carrying randomly inserted activation tag were tested to investigate the growth and biochemical responses to salt stress. Pokkali, IR 29, and non-transgenic Nipponbare were used as tolerant, susceptible, and isogenic control, respectively. Plants were grown in plastic trays filled with 0 g L-1 NaCl Yoshida nutrient solution as control and grown in 6 g L-1 NaCl as treatment. Salt injury was scored on 10, 16, and 21 days after salinization. Observation at 21 days after salinization showed that there was significant difference among genotypes in growth and biochemical characters. Classification using hierarchical cluster analysis based on growth and biochemical responses showed that there were three clusters. Cluster I consisted of wild-type Nipponbare, T2.pMO.V.3.13.c.22, T2.pMO. V.1.7.a.15, T2.pMO.III.98.b.7, T2.pMO.II.231.c15, T2.pMO.III.98.b.8, T2.pMO.III.98.b.17, T2.pMO.III.98.b.3, and T2.pMO.VI.81.3.a.4. Cluster II consisted of IR 29, T2.pMO.V.3.13.c.14, T2.pMO.VI.30.1a.125, and T2.pMO.VI.30.1.a.65. However no mutant genotype was clustered with Pokkali in cluster III, which may indicate that there was no salt tolerant mutant genotype found in this research. Keywords: salt tolerance mutant rice, growth characters, biochemical characters

Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty Rahmawati, Syamsidah; Chairunnisa, Chairunnisa; Erdayani, Eva; Nugroho, Satya; Estiati, Amy
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains also increased significantly (~16-22%). After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress. Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.

Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV. Rojolele and Japonica Rice CV. Gajahmungkur Nugroho, Satya; Loedin, Inez Hortenza Slamet; Ouwerkerk, Pieter B. F.
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Retrotransposons are mobile genetic elements that transpose via an RNA intermediate that is revers transcribed before integration into a new location within the host genome. They are ubiquitous in eukaryotic organisms and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants. Tos17 is a rice endogenous retrotransposon that has been studied thoroughly. Tos17 has been shown to be an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonica rice cv nipponbare. In Javanica rice, however, the presence and activity of Tos17 has not been described thus far. while in some Indica rice Tos17 has been found to be inactive. Javanica rice, also known as tropical Japonica rice, has many cultivars which may serve as potential genetic resources of great interest for breeding programmes. Here, the presence and activities of retrotransposon Tos17 in Javanica rice cv Rojolele was described and compared to those of Japonica rice and Japonica rice cv Gajahmungkur. We identified five and three copies of Tos17 in Rojolele and Gajahmungkur, respectively, with different activities.

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