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All Journal HAYATI Journal of Biosciences Dik Indonesiana CHEMPUBLISH JOURNAL Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Chimica et Natura Acta Bioma : Jurnal Biologi Indonesia Sains Natural: Journal of Biology and Chemistry Jurnal Cendekia : Jurnal Pendidikan Matematika Jurnal Mitra Kesehatan Jurnal Mitra Masyarakat (JMM) BIOVALENTIA: Biological Research Journal Journal Of Research and Education Chemistry (JREC) Jurnal Masyarakat Religius dan Berwawasan Karimah Tauhid
Siti Nurfajriah
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Journal : HAYATI Journal of Biosciences

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Genetic Background of β-Lactamase Genes in Extraintestinal Pathogenic Escherichia coli ST131 in Indonesia Based on Whole-Genome Sequencing (WGS) Sequences Noor Andryan Ilsan; Sipriyadi Sipriyadi; Melda Yunita; Siti Nurfajriah; Maulin Inggraini
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.540-548

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) is a group of pathogens that can colonize the outside of the intestine, such as the kidney, urinary tract, or bloodstream. This study analyzed more about the genetic background of β-lactamase genes among Indonesian ExPEC ST131. Whole-genome sequencing (WGS) sequences from the National Center for Biotechnology Information (NCBI) of Indonesian ExPEC ST131 were taken, then analyzed. Circular genomic mapping and genomic comparison of surrounding genes of β-lactamase in these isolates were generated. blaOXA-1 and blaDHA-1 were analyzed deeply due to their presence in a relatively long contig, making them available for analysis. Indonesian ExPEC ST131 isolates had blaOXA-1 with an identical genetic background of E. coli originating from China and Austria with aac (6’)-1b-cr5 in the downstream and cab83 in the upstream. The blaOXA-1 was also detected in other species, including Klebsiella pneumoniae INF142 originating from Australia and Salmonella enterica S146 from China. While, blaDHA-1 in EC_0406 had an identical genetic background to E. coli 142 and other species such as Shigella sonei FC1428 from India and S. flexneri M2901 from Australia, with Globulin-encoded gene in the upstream and lysR in the downstream. Our findings demonstrate the global spread of β-lactamase genes detected in Indonesian ExPEC ST131. These genes were identical to those isolated from some countries around the world.
Virulence Evaluation of Aeromonas spp. KS-1 Isolated from Kitchen Sponge using Omphisa fuscidentalis Larvae Ilsan, Noor Andryan; Inggrain, Maulin; Nurfajriah, Siti; Yunita, Melda; Priyanto, Jepri Agung; Ramanda, Viqih
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.613-620

Abstract

Aeromonas spp. causes the human diseases including diarrhea, gastroenteritis, and bacteremia. Aeromonas spp. can be found in kitchen sponge, one of the reservoirs for food-borne bacterial pathogens. Virulence study of Aeromonas spp. in vivo in animal model is important since the animal model can mimic manifestasions in human infections. Omphisa fuscidentalis was chosen for alternative virulence model, since they are in the same taxonomical order with the well-known infections model, Galleria mellonella. Bacterial isolation and selection of kitchen sponge used Brain Heart Infusion agar and Endo Agar, respectively. Bacterial virulence of KS-1 was injected into Omphisa fuscidentalis larvae. Survival percentage and melanization score of infected larvae were evaluated. Hemolymph of larvae with melanization score of 1 and 4 were stained with Giemsa method to observe the hemocyte changes. Bacterial identification of isolate KS-1 based on 16S rRNA sequence resulted in 96.9% identity to Aeromonas spp. strain VS7. Isolate KS-1 injection to O. fuscidentalis revealed higher bacterial dosage resulting more severe symptoms to the larvae according to survival percentage and melanization score. However, statistical analysis showed evaluation of melanization score could distinguish larvae with 106 and 107 CFU/larva dosage injection, while evaluation of survival percentage could not. Hemocyte of larvae with melanization score 1 had larger and more cytoplasmic vacuolization than the score 4 (healthy larvae). Omphisa fuscidentalis is an alternative of insect model for bacterial infections with survival percentage and melanization score as the evaluation. Cytoplasmic vacuolization of hemocyte can be used as larvae’s health indicator in a cellular level.
Co-Authors Abd. Rasyid Syamsuri Ahmad Rifans Maulana Amelia, Ria Aprilia, Fina Arsya Putri Janita Dianita Apriyanti DINDHA ARIESTA RAHMA Eka Widana, I Putu Elfira Maya Sari Ilsan, Noor Andryan Iman maulana Inggrain, Maulin Inggraini, Maulin Inggriani, Maulin Intan Kurniawati P Intan Kurniawati Pramitaningrum Jepri Agung Priyanto, Jepri Agung Lestari, Siska Ayu Maulin Inggriani Maulin Inggriani Maulin Inggriani Melania Perwitasari, Melania Melda Yunita Mitha Rianto, Maulidya Nabila rahma sania Neni Arshita Netriwati Noor Adrya Ilsan Pangeran Andareas Putri, Riva Aryani Ramanda, Viqih Rany Widyastuti Rian Permana Saepul Anwar Safitri, Fadliyah Rani Sihotang, Putri Sipayung, Oci Sulastri Sipriyadi Widana, I Putu Eka Yundari, Yundari