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EXPLORATION AND SELECTION OF RHIZOBACTERIA THAT INHIBIT PHYTOPHTHORA CAPSICI IN VITRO Zakia, Aulia; Ilyas, Satriyas; Budiman, Candra; ., Syamsuddin; Manohara, Dyah
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 18, No 1 (2018): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.999 KB) | DOI: 10.23960/j.hptt.11883-94

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Exploration and Selection of Rhizobacteria that Inhibit Phytophthora capsici in vitro. Phytophthora capsici, a seed borne and the soil borne fungal pathogen is the cause of phytophthora blight on chili. The disease is difficult to control because of the resistant varieties unavailability in Indonesia. The aimed was to obtain isolates of rhizobacteria which has the ability to inhibit P. capsici in vitro. Rhizobacteria exploration was conducted in the chili production center in East Java (Malang, Batu, and Kediri) and West Java (Bogor). In one location, chili plant that had symptoms of phytophthora blight disease and a healthy plant next to it were chosen as samples to isolate P. capsici and the rhizobacteria. The rhizobacteria were isolated on NA, TSA, and TSAP (TSA with heated sample). Samples of diseased plants were used in isolation of P. capsici on V8 agar. The inhibition and compatibility of the rhizobacteria to inhibit P. capsici in vitro were tested by dual culture method. In this experiment, it was obtained 252 isolates of rhizobacteria and one isolate of P. capsici. Isolates of rhizobacteria with high to medium inhibition were E1, E3C2, and F2B1 respectively. All three isolates were then combined and tested against P. capsici in vitro. The highest inhibition was indicated by four isolate and combination of isolates, which were E1 isolate (58%), the combination of E1 + E3C2 isolates (58%), E1 + F2B1 (60%) and E1 + E3C2 + F2B1 (58 %).
Determination of Agronomic Characteristics as Selection Criteria in Potato Crossing Lines Ishartati, Erny; Sufianto, Sufianto; Zakia, Aulia; Mejaya, Made Jane; Madianto, Rudy
PLANTA TROPIKA Vol 10, No 1 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.7571

Abstract

Vegetative propagation of potato decreases variation in commercially cultivated varieties. Induction of variability in potatoes is needed for crop improvement. Selection is selecting the best individual plants based on the desired characteristics, which will be effective when suitable characteristics are used. The objective of this study was to determine the agronomic characteristics for the selection of potato crossing lines through heritability, genetic variability, and genotypic correlation. The experiment was conducted at the Greenhouse, Sumber Brantas Village, Batu City, East Java. The materials used were 30 potato lines resulting from LJPRSD1 x AP-4. This study was arranged in a complete randomized design (RCD) with three replications. The agronomic characteristics observed include plant height, stem diameter, number of leaves, the diameter of tuber, number of tubers, tuber length, weight per tuber, starch, and glucose. Almost all characteristics observed showed high heritability values, except stem diameter and tuber length. The genetic variability in stem diameter, tuber diameter, and tuber length was low, whereas the genetic variability in other characteristics was slightly low. The genotypic correlation was significant between characteristics, except between starch content and weight per tuber. Meanwhile, the agronomic characteristics used for selection are plant height, diameter of tuber, number of tubers.
Modifikasi Teknik Invigorasi untuk Meningkatkan Viabilitas dan Vigor Benih Jagung Manis (Zea mays Sacharata L.) Aulia Zakia; Muhammad Bahrul Ulum; Aniek Iriany; Agus Zainudin
Agriprima : Journal of Applied Agricultural Sciences Vol 5 No 1 (2021): MARET
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v5i1.383

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Penyimpanan benih yang salah, dapat menyebabkan deteriorasi, sehingga viabilitas dan vigor benih di lapangan rendah. Teknik invigorasi diterapkan sesuai dengan jenis benihnya. Penelitian ini bertujuan untuk mengetahui apakah media agar yang dicampur dengan arang aktif dapat digunakan sebagai alternatif cara invigorasi benih Jagung manis. Penelitian dilaksanakan pada bulan Oktober-November 2019, bertempat di Laboratorium Benih Agronomi, Program Studi Agroteknologi, Fakultas Pertanian-Peternakan, Universitas Muhammadiyah Malang. Penelitian dilakukan pada benih Jagung manis varietas Talenta, menggunakan rancangan acak lengkap (RAL) sederhana. Modifikasi teknik invigorasi sebagai faktor perlakuan, terdiri atas Kontrol, benih ditumbuhkan dalam media pasir (A0), modifikasi media agar (A1), modifikasi media agar ditambah arang aktif (A2), modifikasi media agar yang ditumpuk/ benih diletakkan diantara media agar (A3), modifikasi media agar+arang aktif yang ditumpuk/ benih diletakkan diantara media agar+arang aktif (A4), matriconditioning menggunakan arang sekam (A5). Setiap perlakuan diulang sebanyak 3 kali. Hasil percobaan menunjukkan bahwa viabilitas dan vigor benih Jagung masing-masing meningkat sebesar 22,22 % dan 19, 45 %. Modifikasi metode invigorasi menggunakan agar dan arang aktif dapat dijadikan sebagai alternatif perlakuan invigorasi terhadap benih Jagung.
Increased Agricultural Literacy through the Bacterial Consortium Production Based on IoT Temperature Sensors for Students at SMKN 1 Purwosari: Peningkatan literasi pertanian melalui produksi konsorsium bakteri berbasis IoT sensor suhu pada siswa SMKN 1 Purwosari Erfan Dani Septia; Maftuchah Maftuchah; Aulia Zakia; Iqbal Maulana Zulfan; Akhmad Rizal Oktafian; Sohnif Nurwicahyo Putra
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 2 (2024): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v8i2.17691

Abstract

This community service program aims to enhance the understanding of Vocational High School (SMK) students in sustainable agriculture, particularly in producing bacterial consortiums of Biofertilizer and Bioprotectant. Situation analysis reveals that SMK students face challenges in accessing training, limited resources and facilities, and a lack of awareness regarding the importance of sustainable farming practices. The program presents strategic solutions, beginning with developing a comprehensive learning module on sustainable agriculture and utilizing bacterial consortiums, biofertilizers, and bioprotectants. This module is designed to assist students in comprehending the concepts, benefits, and practical applications of sustainable farming. The solution also includes practical field training, especially in the propagation of bacterial consortiums, utilizing IoT temperature sensor DS18B20 to monitor the propagation process. The anticipated outcome of this program is an improvement in sustainable agricultural literacy among SMK students. The expectation is that they will better understand the concepts of sustainable agriculture and the implementation of bacterial consortiums, biofertilizers, and bioprotectants. Additionally, students are expected to acquire practical skills in the propagation of bacterial consortiums. Thus, the program aims to positively contribute to developing students' skills and encourage active participation in sustainable agriculture.
Quality and resistance optimization of banana (Musa acuminata L.) vegetative seeds through the addition of indole butyric acid with biological agents induction against fusarium wilt disease intensity Septia, Erfan Dani; Zakia, Aulia; Zainudinn, Agus; Maftuchah, Maftuchah; Gofur, Abdul; Abbabiel, Yudantara Ekanaradhipa
Kultivasi Vol 23, No 2 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i2.50966

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Banana plants are a vital agricultural commodity in Indonesia, but Fusarium wilt disease (Fusarium oxysporum) significantly hampers production. This research aims to improve the quality and resilience of vegetative banana seedlings by selecting superior seedlings and vegetative propagation techniques such as budding, shoot cutting, and tissue culture management. The research used a randomized complete block design (RCBD) factorial with a combination of two factors, namely the addition of IBA and biological agents, with nine treatment combinations and three replications. IBA treatment significantly affected the initial emergence of shoots and leaf area, with the best result from treatment Z2 (IBA concentration of 1.5 g/l). The biological agent factor treatment significantly affected plant height, lower stem diameter, number of leaves, and hypothetical vigor index, with the best value shown by treatment B1. The use of IBA and biological agents can optimize the quality and resilience of vegetative banana seedlings, aiding farmers in enhancing the productivity and quality of agricultural products sustainably.
Seed Priming Used Coconut Water in Different Concentration and Soaked Periode to Increase Soybean Germination Wibowo, Anang; Zakia, Aulia; Sufianto
Journal of Tropical Crop Science and Technology Vol. 3 No. 2 (2021): VOLUME 3, NO. 2, OCTOBER 2021
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v3i2.29719

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Soybean seeds are orthodox seeds that quickly deteriorate, especially if the storage environment conditions are less favorable (sub optimum). Soaking using coconut water can be done to overcome the deterioration of soybean seeds by utilizing vegetable waste. The purpose of this research was to study the effect of concentration and duration of coconut water soaking on the process of soybean seed germination. This research was conducted in January-February 2020, at the Agronomy Laboratory, Agrotechnology Study Program, Faculty of Agriculture-Livestock. The soybean used in the study was the Dena I variety. Factorial Completely Randomized Design (CRD) with 2 factors was used in this study. Factor 1 was coconut water concentration (K0 = 0% Coconut Water Concentration; K1 = 25% Coconut Water
The Effect of the Administration of Calcium Chloride (CaCl2) and Phosphoric Acid Fertilizer (H3PO4) on the Growth and Production of Strawberry (Fragaria ananassa) Rasyid, Harun; Zakia, Aulia; Triyuana, Resty Mirza
Journal of Tropical Crop Science and Technology Vol. 3 No. 1 (2021): VOLUME 3, NO. 1, MARCH 2021
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v3i1.29733

Abstract

Strawberry production in Indonesia has been on the rise in various regions, including Aceh, Riau, Jambi, DKI Jakarta, West Java, Central Java, East Java, and others. According to the Central Bureau of Statistics (2021), production fluctuated from 573.00 tons in 2019 to 559.00 tons in 2020, rebounding to 838.00 tons in 2021. Factors influencing production include land availability, proper fertilization methods, and planting media. Adequate fertilization, considering both dosage and frequency, plays a crucial role. The study, conducted at Balitjestro GreenHouse in Batu City, East Java, focused on the interaction between lime and fertilizer on strawberry plant growth and yield. Synthetic fertilizers, particularly phosphate fertilizers, are essential for plant growth. The research utilized a completely randomized design with two factors: CaCl2 lime (S) and phosphoric acid fertilizer (R), resulting in two treatment combinations (4x4). Each treatment, repeated three times, yielded 48 experimental units, with one plant per unit. Results indicated that the addition of CaCl2 lime and phosphoric acid fertilizer had a minimal impact on strawberry plant growth and production. Specific combinations, such as calcium chloride and phosphoric acid fertilizer with KCL 7.2 grams and urea 12.5 grams, did not significantly affect vegetative growth parameters like plant height and leaf count. However, these combinations did influence strawberry plant production. The study underscores the need for careful nutrient management to optimize strawberry production in Indonesia.
Testing the Effectiveness of Biological Agents as Biofertilizers on the Growth of Two Rice Varieties (Oryza sativa L.) in Screen House Gibran, Rizki Nabil; Nursandi, Fatimah; Zakia, Aulia
Journal of Tropical Crop Science and Technology Vol. 3 No. 2 (2021): VOLUME 3, NO. 2, OCTOBER 2021
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v3i2.29743

Abstract

Rice (Oryza sativa L.) is one of the most important food commodities for human nutritional needs. The amount of rice consumption per capita per week in 2017 was 1,571 (BPS, 2018). Population growth over time has resulted in increased demand for rice in Indonesia. Therefore, the demand for rice by the Indonesian people is very high. The problem is that the demand for local rice in Indonesia is still low, so one way to meet the demand for rice in Indonesia is through imports. Utilization of water and land resources is one of the common challenges faced in realizing national food security (Fita et al., 2013). Low productivity of rice plants is generally caused by biological, physical, and chemical soil problems. One of the efforts to increase the productivity of rice plants is by utilizing biological resources such as biofertilizers.The objectives of this study were to determine the appropriate dose of biofertilizer that will affect the growth of rice plants, determine the combination of biofertilizer and application interval that affects the growth of rice plants, and determine the effect of biofertilizer application on 2 varieties of rice plants (Ciherang and IR 64). The design used in this study was a factorial complete randomized block design (RKLT) to determine the interaction and effect of each factor. Then DMRT (Duncan's test) was conducted to determine the best treatment. Based on the results of the study, it was found that the interaction between biofertilizer treatment and rice varieties had significant effects on almost all observed variables except stem diameter at 70 HST, number of clumps at 42 HST, number of productive tillers, and weight of planted seeds. The combination of biofertilizer treatment and rice variety had inconsistent effects on growth parameters and rice yield.
Teknologi Produksi Benih Kentang untuk Mewujudkan Kemandirian Benih di BUMDes Desa Batur Dr. Syarif Husen; Agus Eko Purnomo; Aniek Iriany; Muhidin Muhidin; Aulia Zakia; Iqbal Ramadhani Fuadiputra; M. Zul Mazwan; Farusa Anggita; Rizka Nurfitriani
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 1 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.1.12-22

Abstract

The area at the foot of Mount Merbabu, more precisely in Batur Village, has an altitude of 1,200‒1,800 meters above sea level, so it is very suitable for cultivating potato plants. Several farmers have cultivated this, but the production results are not always optimal. The potato crop productivity problems faced by farmers in BUMDes are: 1) Limited quality seeds; 2) The price of seeds is high and difficult for farmers to reach; 3) Farmers use seeds from some crops that are not of good quality so the yield is low and susceptible to disease; and 4) Farmers are unfamiliar with the technology for producing superior potato seeds. The University of Muhammadiyah Malang has superior virus-free seed sources and technology that can be adopted and disseminated to farmers to produce quality seeds independently so that they can overcome the problems they face. Activities are carried out in a participatory manner using tutorial methods, training and demonstration plots at locations, and group mentoring. Partners agree to actively provide human resources, necessary facilities, and accompanying funds in implementing activities. At this time, farmers have understood planet acclimatization technology. They can produce 5,000 mini cuttings from 500 acclimatized broodstock, 10,000 G0 seeds, and 25 tonnes/ha of G2 seeds using rooted shoot-cutting technology. Additionally, partner farmers have obtained permits as seed producers from BPSB (Seed Supervision and Certification Center). This program is ongoing, and UMM will assist on an ongoing basis until the establishment of an independent BUMDes for potato seeds in Semarang Regency.
Peningkatan Pertumbuhan Tanaman Cabai dan Pengendalian Busuk Phytophthora melalui Biopriming Benih dengan Rizobakteri Asal Pertanaman Cabai Jawa Timur Aulia Zakia; Satriyas Ilyas; Candra Budiman; Syamsuddin ,; Dyah Manohara
Jurnal Hortikultura Indonesia (JHI) Vol 8 No 3 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.117 KB) | DOI: 10.29244/jhi.8.3.171-182

Abstract

ABSTRACT The objectives of this study was to evaluate biopriming of chili seed with rhizobacteria to improve plant growth and control Phytophthora blight disease in a greenhouse. This experiment used three isolates of rhizobacteria, i.e. E1, E3C2 and F2B1, and isolate Phytophthora capsici (Cb6) isolated from the production center of chili in East Jawa. Laris variety from PT. East West was used in this experiment. This experiment used randomized block design with one factor, i.e. 11 levels of seed treatment (E1 rhizobacteria, E3C2 rhizobacteria, F2B1 rhizobacteria, E1+E3C2 rhizobacteria, E1+F2B1 rhizobacteria, E1+E3C2+F2B1 rhizobacteria, seed soaking in water, without soaking, metalaxyl, positive control and negative control). The result showed that seed treatment with combination of E1+F2B1 isolates when grown in nursery, significantly increased the height and number of leaves in chilli. Besides, seed treatment with F2B1 isolate and combination of E1+F2B1 isolates after transplanting were capable to improve plant growth and control Phytophthora blight disease in greenhouse.Keywords: greenhouse, isolate rhizobacteria, Phytophthora capsici  ABSTRAK Tujuan penelitian ini ialah mengevaluasi perlakuan biopriming benih cabai dengan rizobakteri dalam meningkatkan pertumbuhan bibit dan mengendalikan kejadian busuk Phytophthora di rumah kaca. Perlakuan biopriming benih dengan rizobakteri menggunakan tiga isolat rizobakteri E1, E3C2 dan F2B1 dan isolat Phytophthora capsici Cb6 hasil eksplorasi pertanaman cabai Jawa Timur. Benih yang digunakan dalam percobaan merupakan benih varietas Laris produksi PT. East West. Percobaan menggunakan rancangan acak kelompok satu faktor, masing-masing perlakuan diulang empat kali, dengan 11 taraf perlakuan, antara lain R0+ (kontrol positif, benih direndam dalam PDB tanpa perlakuan rizobakteri dengan inokulasi P. capsici), R0- (kontrol negatif, benih direndam dalam PDB tanpa perlakuan rizobakteri dan tanpa inokulasi P. capsici), R1 (perlakuan benih dengan isolat E1), R2 (isolat E3C2), R3 (isolat F2B1), R4 (kombinasi isolat E1+E3C2), R5 (kombinasi isolat E1+F2B1), R6 (kombinasi isolat E1+E3C2+F2B1), R0RA (benih direndam dalam air 24 jam), R0TR (benih tanpa rendam), R0M (benih direndam dalam metalaksil). Tanah inokulum P. capsici diberikan 28 hari setelah pindah-tanam di sekitar pangkal batang tanaman cabai di bawah permukaan tanah. Hasil percobaan menunjukkan, perlakuan dengan kombinasi isolat E1+F2B1 saat persemaian di rumah kaca nyata meningkatkan tinggi dan jumlah daun tanaman cabai. Perlakuan benih dengan isolat F2B1 maupun kombinasi isolat E1+F2B1 setelah pindah-tanam di rumah kaca memiliki kemampuan meningkatkan pertumbuhan tanaman serta mengendalikan penyakit busuk Phytophthora. Kata kunci: isolat rizobakteri, Phytophthora capsici, rumah kaca