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All Journal Jurnal Tumbuhan Obat Indonesia PROSIDING SEMINAR KIMIA Biopropal Industri Jurnal Kimia Mulawarman BERITA BIOLOGI Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Jurnal Kimia Terapan Indonesia FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Ilmu Kefarmasian Indonesia Pharmaceutical And Biomedical Sciences Journal (PBSJ) Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Partomuan Simanjuntak
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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AKTIVITAS DAN ISOLASI SENYAWA BIOAKTIF DARI EKSTRAK ETIL ASETAT DAUN TEH (Camellia sinensis (L.) Kuntze) SEBAGAI PENGHAMBATAN ENZIM α-GLUKOSIDASE Nuraini Saadah; Lilik Sulastri; Syamsoedin Abdilah; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Tea (Camellia sinensis (L.) Kuntze) is one of the plants that has compounds that have the potential as inhibitors of the α-glucosidase enzyme. The purpose of this study was to know the activity of chemical compounds in the ethyl acetate extract of tea leaves as an inhibitor of the α-glucosidase enzyme. Extraction tea leaf was carried out by maceration tea leaf powder in ethanol 96% 3 times, then partitioned with ethyl acetate and water. The ethyl acetate extract obtained was determined by the inhibitory activity of the -glucosidase enzyme, then fractionated by column chromatography (SiO2; n-hexane: ethyl acetate 10:1 ~ 1:1) yielding 10 fractions (CSEA-1 ~ CSEA-10). The CSEA-2 fraction was further fractionated by column chromatography (SiO2; n-hexane: ethyl acetate 20:1 ~ 1:1) yielding 9 fractions (CSEA-2.1~ CSEA-2.9). The results showed that the ethyl acetate extract had activity with an IC50 value of 45.04 ppm, the fractionated CSEA-2.1 subfraction had an IC50 value of 41.51 ppm with acarbose as a comparison of 19.24 ppm. The conclusion is that the CSEA-2.1 subfraction has better activity than the extract in inhibiting the α-glucosidase enzyme and can be used as an alternative candidate for diabetes drugs. Keywords: Camellia sinensis (L.) Kuntze, tea, α-glucosidase enzyme inhibitor, Indonesian medicinal plant, IC50
AKTIVITAS DAN ISOLASI SENYAWA AKTIF PENGHAMBAT ENZIM ALFA-GLUKOSIDASE DARI EKSTRAK ETIL ASETAT DAUN STEVIA (Stevia rebaudiana Bertoni) SECARA IN VITRO Laura Stephanie Joner; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Stevia leaves (Stevia rebaudiana Bertoni) is a plant that commonly used as a natural sweetener to replace sugar. According to research, stevia has many health benefits and one of them is antidiabetic. The aims of this research are to determine the activity of the ethyl acetate extract of stevia leaves (Stevia rebaudiana Bertoni) as an inhibitor of -glucosidase enzyme. The stages on this research include extraction with 96% ethanol solvent, then the ethanolic extract was partitioned with ethyl acetate – water = 1:1. Isolation and purification of the ethyl acetate extract was carried out by column chromatography (SiO2; i). n-hexane – etil acetate = 10 : 1 ~ 1 : 1 ii). n-hexane – etil acetate = 20:1 which was guided by testing the inhibitory activity of α-glucosidase enzyme by in vitro studies. The results showed that the STEA-1.1 fraction had the best activity with IC50 value 39,55 ± 0,51 ppm compared to the partitioned ethyl acetate extract with an IC50 value 43,47 ± 0,05 ppm, while the acarbose had an IC50 value 19,24 ± 0,03 ppm. Keywords: Stevia rebaudiana Bertoni, α-glucosidase, Antidiabetic, Indonesian Medicinal Plants, IC50
UJI AKTIVITAS ANTIMIKROBA HASIL BIOPRODUKSI KAPANG ENDOFIT DAUN LIDAH MERTUA (Sansevieria trifasciata Prain) Lea P. Retnoningsih; Shirly Kumala; Marlin M. Raunsai; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 1 (2022): Prosiding SNK 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Secondary metabolite from Lidah mertua’s leaves (Sansevieria trifasciata Prain) had reported antimicrobial activity. The aim of this study is to determine the fractions of secondary metabolite produced by endophytic fungi of Lidah mertua’s leaves which potential to be antibacterial agent against Staphylococcus aureus and Escherichia coli. Rejuvenated endophytic fungi in Potato Dextrose Agar (PDA) fermented in Potato Dextrose Yeast (PDY) for 14 days, extracted with ethyl acetate obtained F.A and F.B extract from filtrat and B extract from biomass. F.A and B extract fractionated with coloumn chromatography ( SiO2; n-heksan – ethyl acetate and dichlorometana – methanol) obtained 8 fractions from filtrate extract (F.A-1 ~ F.A-8) and 5 fractions from biomass extract (B-1 ~ B-5). Anti-pathogenic bacterial tests used the KLT-bioautography dot-blot and elusion methods. Fraction F.A-1 ~ F.A-8 and F.B extract showed antimicrobial activity against S. aureus InaCC-B4 and E. coli InaCC-B5. Fraction B-1 ~ B-5 showed antimicrobial activity against S. aureus but the B-5 fraction were not showed antimicrobial activity againts E.coli. The fraction that has the potential to be a broad-spectrum antibacterial is the fraction F.B. Keywords: antimicrobial activity test, bioproduction, endophytic fungi, Lidah mertua’s leaves, Sansevieria trifasciata Prain.
AKTIVITAS SENYAWA BIOAKTIF DARI EKSTRAK ETIL ASETAT DAUN YAKON (Smallanthus sonchifolius) SEBAGAI PENGHAMBAT ENZIM α-GLUKOSIDASE Riska Diana; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Yakon (Smallanthus Sonchifolius) leaves in Indonesia, better known as insulin leaves, scientifically have various properties and one of them is antidiabetic. This study aims to determine the activity of secondary metabolites contained in the ethyl acetate extract of yakon (Smallanthus Sonchifolius) leaf as an inhibitor of the -glucosidase enzyme. Yakon leaf ethyl acetate extract using eluent from TLC results. Until several sub-factions are obtained. These sub-fractions were combined in TLC and tested for activity. The sub-fraction with the best activity in Back column chromatography and TLC and tested until the IC50 value is obtained. The results showed that the ethyl acetate extract of yakon leaves had inhibitory activity of the -glucosidase enzyme with an IC50 of 45,67 ppm. The results of column chromatography separation showed that sub-fraction 6.5 was a sub-fraction with an IC50 value of 42,23 ppm. Conclusion: Yakon leaf ethyl acetate extract had better activity than purified F6.5 and acarbose as a positive control with an IC50 value of 21,36 ppm. Keywords : Smallanthus sonchifolius, α -glucosidase inhibitory enzyme, IC50, Indonesian medicinal plant
AKTIVITAS PENGHAMBATAN ENZIM α-GLUKOSIDASE EKSTRAK DAN FRAKSI ETIL ASETAT DAUN SALAM (Syzygium polyanthum (Wight) Walp.) Aufa Ali Fajar Nanda Nasukha; Lilik Sulastri; Partomuan Simanjuntak
PROSIDING SEMINAR KIMIA Vol 1 No 2 (2023): Prosiding SNKT II 2022
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Diabetes Mellitus is a chronic disease caused by the inability of the pancreas to produce insulin which is characterized by hyperglycemia. One of the therapies used to lower blood glucose levels is drugs that have a mechanism to inhibit the α-glucosidase enzyme, so that it can delay the glucose absorption in the digestive tract. One of the plants that has the potential to inhibit the α-glucosidase enzyme is bay leaf. The intention of this study was determine the activity of ethyl acetate extract and chromatographic fraction of bay leaf in inhibiting the α-glucosidase enzyme. A liquid-partition process from 96% ethanol extract was carried out to obtain ethyl acetate extract. The ethyl acetat extract was separated by fractionation using chromatography and TLC with pNPG substrate and acarbose positive control. The inhibitory activity testing of the α-glucosidase enzyme was executed using an ELISA reader with a wavelength of 405 nm. The result revealed that the ethyl acetate extract had inhibitory activity of the α-glucosidase enzym with an IC50 of 79,65 ppm. The F-5.5 fraction was the best fraction with an IC50 value of 18,99 ppm. The acarbose activity in inhibiting the α-glucosidase enzyme had an IC50 value of 18,05 ppm. Keywords : α-glucosidase, antidiabetic, Salam leaves, Syzygium polyanthum
Penambatan Molekul Senyawa Swietemacrophyllanin dari Kulit Batang Mahoni (Swietenia macrophylla King,) sebagai Inhibitor Enzim Alfa Glukosidase Arini Khaerunnisa; Ratna Djamil; Lilik Sulastri; Tarso Rudiana; Partomuan Simanjuntak
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 5, No 2 (2023)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v5i2.32926

Abstract

Mahogany (Swietenia macrophylla King.) is one of the plants used as an antidiabetic agent. 96% ethanol extract of S. macrophylla stem bark can inhibit the α-glucosidase enzyme. Methanol and aqueous extracts of S. macrophylla stem bark can reduce rat blood glucose levels induced by streptozotocin. Molecular docking is one of the most widely used structural-based drug design strategies due to its wide application in the analysis of binding energy and molecular interactions between drug compounds and receptors. This study aims to identify the compounds in the stem bark of S. macrophylla and analyze the molecular anchoring of the compounds produced by the aid. The research was carried out by means of the water fraction of the stem bark of S. macrophylla and was purified by column chromatography. Fraction 2 was identified by LCMS-MS. Molecular docking using AutoDock 4.2.6. The results of Fraction 2 showed the presence of catechins, evodionol, swietemacrophyllanin, swietenitin K and β-sitosterol. The results of an in-silico study showed that swietemacrophyllanin provided a better affinity than acarbose with a binding energy value of -8.57 kcal/mol and an inhibition constant of 0.52 µM. Forms hydrogen bonds with catalytic residues (Glu277 and Asp352) from the active site of the α-glucosidase enzyme. Swietemacrophyllanin has potential as an antidiabetic agent
SENYAWA KIMIA STEROL DARI KULTUR JARINGAN KELADI TIKUS (Typhonium divaricatum Decne) Lilik Sulastri; Partomuan Simanjuntak
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung sekolah tinggi ilmu kesehatan samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v5i2.264

Abstract

Keladi tikus (Typhonium divaricatum Decne) is one of Aracaceae family which a plant that’s very popular in Indonesian society because it contains anti-cancer substances. Nowadays, keladi tikus plants have been cultivated in tissue culture widely. The simplicia used in this study was keladi tikus from tissue culture which was separated into the upper part (leaves and petioles) and the bottom (tubers) dried in the sun. Dry simplicia was extracted with methanol at a temperature of 60-70 oC and the methanol extract was partitioned with ethylacetate then with n-BuOH solvents, and all extracts were tested for cytotoxic tests using the Brine Shrimp Lethality test (BSLT) method. The active fraction of ethylacetate extract, which is the tuber parts was carried out by column chromatography (SiO2; i). n-hexane: ethylacetate = 10: 1 ~ 1: 1; ethylacetate; MeOH; ii). n-hexane-ethylacetate = 5: 1) give one pure isolate. The results of identification based on Infrared (IR) spectra, and gas chromatography-mass spectra (GC-MS) are b-sitosterol which LC50 is 76.2 ppm
PENGHAMBATAN AKTIVITAS ENZIM α-GLUKOSIDASE DARI EKSTRAK AIR TANAMAN RARU (Vatica pauciflora) SECARA IN VITRO eris septiana; wiwi winarti; partomuan simanjuntak
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 4 No. 1 (2017)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/farmasains.v4i1.96

Abstract

Diabetes mellitus is a metabolic disorder that leading the blood sugar levels become higher than normal. In Indonesia, one of the plants that traditionally used to cure hyperglycemic is raru plant (Vatica pauciflora). The objectives of this research was to investigate α-glucosidase inhibitory activity from stem bark, root, and leaf of raru plant. Extraction of stem bark, root, and leaf was conducted with reflux at 70 °C by using water as a solvent. In vitro anti-diabetic activity was tested using the method of α-glucosidase inhibition. The results show that water extracts of raru's stem bark, root, and leaf have α-glucosidase inhibitory activity. The stem bark water extract has the highest activity than leaf and root with IC50 values were 13.53, 16.96, and 41.91 ppm respectively. All extracts is categorized as active in inhibiting the activity of α-glucosidase enzyme by having IC50≤100 ppm. The results obtain in this research clearly indicate a promising potential as anti-diabetic properties of raru plant.
Co-Authors Abdul Malik Anwar, Yelfi Arini Khaerunnisa Aufa Ali Fajar Nanda Nasukha Binawati Ginting Bustanussalam Bustanussalam Danty Nur Alvionitasari Edi Susanto Eka Damayanti Setyningrum Elvina Dhiaul Iftitah Enny Sudarmonowati Eris Septiana Eris Septiana Eris Septiana Fauzia Nurul Izzati Fauzy Rachman Fauzy Rachman Fauzy Rachman Harmastini I. Sukiman Lamek Marpaung Laura Stephanie Joner Lea P. Retnoningsih Leny Heliawati Lilik Sulasti Lilik Sulastri Marlin M. Raunsai Mastura Mastura Murniaty Simorangkir Nico Satria Salim Nuraini Saadah Pandapotan Nasution Rahmawati, Siti Irma Ratna Djamil Ribu Surbakti Riska Diana rizky maulida amalia hanif Rudi Kartika, Rudi Rutdianti Rutdianti Shirly Kumala Sri Hartati Syamsiah Syamsiah Syamsoedin Abdilah Syamsudin Syamsudin Sylvia J.R. Lekatompessy Tarso Rudiana, Tarso Titik K. Prana Tonel Barus Wien Kusharyoto Wiwi Winarti Yashinta Larasati Yatri Hapsari Yesi Desmiaty, Yesi