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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Prosiding Seminar Biologi AL KAUNIYAH Jurnal Bidan Prada Biota Biology, Medicine, & Natural Product Chemistry INDONESIAN JOURNAL OF PHARMACY Jurnal Penelitian Pertanian Terapan BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biodjati Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Life Science JURNAL BIOLOGI PAPUA
Erma Prihastanti
Department Of Biology, Faculty Of Science And Mathematics, Universitas Diponegoro, Jl. Prof. Sudarto, S.H. Tembalang, Semarang 50275, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE Prihastanti, Erma; Soegihardjo, C. J.; Purbaningsih, Susiani
Indonesian Journal of Pharmacy Vol 12 No 1, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.652 KB) | DOI: 10.14499/indonesianjpharm0iss0pp10-19

Abstract

Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Co-Authors Afrazak Johansyah Agus Subagio Agus Subagyo Ahmad Fuad Masduqi Anitasari, Emi Arif Surahman Ariyanti Ariyanti Ariyanti Ariyanti Ayu Rahmawati Sulistyaningtyas C. J. Soegihardjo Chatarina Umbul Wahyuni Chory Praseptiana Christoph Leuschner Della Widya Puspita Della Widya Puspita Desy Wulan Triningsih Devi Ermawati Dhitya Arlan Bayu Martha Didie Soepandi Didy Sopandie Diwyacitta Prasasti Emi Anitasari Endah D. Hastuti Endah Dwi Astuti Endah Dwi Hastuti Endang Kusdiyantini Endang Saptiningsih Esti Meita Kridati Fajar Arianto Faradis Ulyah Fatkhu Zaimah Febiasasti Trias Nugraheni Fifit Yuniati Harmigita Putri Fitriani Hastuti, Endah Helmiyesi Helmiyesi I’anatushshoimah, I’anatushshoimah IBNUL QAYIM Ika Dyah Kumalasari Ika Puspitasari I Ilma Khaerasani Imam Firmansyah Imam Firmansyah Indriani, Reni Jinus Jinus Jumari Jumari, Jumari Junita Junita Kasiyati Kasiati Ken Qudsy Royana, Ken Qudsy Kuwati Kuwati Lily Nur Inda Sary Lutfiyatul Wahdah Mega Rizqi Utami Monika Heti Nuryana Much Azam Much Azam Mudlikatun Khasanah Muhammad Agus Muljanto Muhammad Luthfi Munifatul Izzati Munirotun Roiyana Ngadiwiyana M.Si. S.Si. Ningrum, Dyah Ayu Kusuma Nintya Setiari Niti, Mahatma Narendra Noor Laila Safitri Nurrahmah Azizah, Nurrahmah Oktivani D.P. Hayati Puji Hastuti Putri Zulaida Ningtyas Rafif Zuhair Muhammad Regina Cahya Cendekianesti Retno Robiatul Al Adawiyah Rini Budi Hastuti Rini Budi Hastuti Rini Budihastuti Robi’atul Asifah Sarjana Parman Sepsamli, Letus Silvana Tana Soekisman T SOEKISMAN TJITROSEMITO Sofian, Arif Ardianto Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sugiyatno Sugiyatno Sulastri Diana Silaban Surahman, Arif surur, Mukhammad akmal Susiani Purbaningsih, Susiani Tyas Rini Saraswati Ulva, Maria Wijaya, Panca Buana Winangsih Winangsih Yulita Nurchayati Zaenul Muhlisin Zaenul Muhlisin Zelly Fujianto, Zelly Zelly Fujiyanto, Zelly