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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Kimia (Journal of Chemistry) E-Jurnal Medika Udayana Universa Medicina International Journal of Health and Pharmaceutical (IJHP) Jurnal Riset Rumpun Ilmu Kedokteran (JURRIKE)
Ida Bagus Nyoman Putra Dwija
Departement Of Clinical Microbiology Udayana University - Prof,Dr,I.G.N.G Ngoerah General Hospital, Denpasar, Bali, Indonesia
Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Article Review: Phenotypic and Genotypic Identification of Bacillus cereus in Soy Milk Ni Ketut Sastraningsih; I Putu Bayu Mayura; Ida Bagus Nyoman Putra Dwija
International Journal of Health and Pharmaceutical (IJHP) Vol. 6 No. 2 (2026): May 2026
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v6i2.633

Abstract

Bacillus cereus  is a Gram-positive, spore-forming bacterium frequently found as a contaminant in various food products, including soy milk, and is recognized as a causative agent of foodborne illnesses. Its pathogenicity mainly arises from the production of toxins such as non-hemolityc enterotoxin (nhe), hemolysin BL (hbl), and cytotoxin K (cytK), which contribute to gastrointestinal disorders. Conventional phenotypic identification methods, including culture, morphological observation, and biochemical assays, are simple but often lack specificity in distinguishing B. cereus from the B. cereus group. Therefore, genotypic approaches based on molecular techniques such as PCR and Whole Genome Sequencing (WGS) are required to achieve higher sensitivity and precision. The gyrB gene serves as a molecular marker for species confirmation, while the nhe gene complex acts as a virulence determinant in identifying toxigenic strains. The integration of phenotypic and genotypic methods provides a more comprehensive understanding of B. cereus contamination in soymilk, elucidating its genetic diversity and pathogenic potential, which are essential aspects of food safety assessment.
Co-Authors Gede Wirya Diptanala Putra Duarsa I Dewa Made Sukrama I Gusti Ayu Agung Elis Indira I Gusti Ayu Nastiti Wahyu Handayani I Ketut Agus Somia I Ketut Juniarta I Putu Bayu Mayura Kadek Dwi Adi Mas Putrayana Ketut Tuti Parwati Merati Komang Vindya Triananda Made Agus Hendrayana Made Yogi Oktavian Prasetia Mita Anggraeni, Mita Ni Ketut Sastraningsih Ni Made Dewi Dian Sukmawati Ni Putu Ariantari Ni Putu Kusuma Santhi Y Ni Wayan Winarti Nyoman Gilang Putrayasa Putu Agoes Adi Ariestyawan Nugraha Putu Bagus Redika Janasuta Putu Harrista Indra Pramana Rinawati, Luh Putu Sagun Chandra Yowani