Article Per Year (5 Year)
p-Index From 2021 - 2026
1.728
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Biotechnology Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Prosiding University Research Colloquium
DIAH RACHMAWATI
Staf Pengajar Fakultas Biologi UGM
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
Pengaruh Aplikasi Pupuk Nitrogen terhadap Ketahanan Tembakau (Nicotiana tabacum L. ‘Manilo’) pada Cekaman Kekeringan Ardadi, Maharani Pratiwi Salsabila; Nurbaiti, Siti; Rachmawati, Diah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.11663

Abstract

Tembakau (Nicotiana tabacum L.) merupakan salah satu tanaman unggulan yang berkontribusi dalam perekonomian Indonesia. Namun, perubahan iklim dapat memicu berbagai cekaman abiotik, seperti kekeringan yang dapat menghambat pertumbuhan tanaman. Salah satu upaya yang dapat dilakukan untuk meningkatkan toleransi tanaman terhadap kekeringan adalah melalui pemberian pupuk nitrogen. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian pupuk nitrogen terhadap ketahanan tembakau (Nicotiana tabacum L. ‘Manilo’) pada cekaman kekeringan. Penelitian dilakukan menggunakan metode Rancangan Blok Acak Lengkap dengan perlakuan kombinasi tiga tingkat ketersediaan air (100%, 75%, dan 50% kapasitas lapang) serta tiga dosis pupuk nitrogen (0,6; 1,2; dan 1,8 g/polybag). Parameter yang diamati meliputi respons tingkat kerusakan serta aktivitas antioksidan enzimatik. Analisis data dilakukan menggunakan ANOVA dan uji DMRT pada taraf kepercayaan 95%. Hasil menunjukkan aplikasi pupuk nitrogen mampu meningkatkan aktivitas enzim antioksidan, terutama APX. Peningkatan dosis pupuk nitrogen berkorelasi positif dengan ketahanan tanaman terhadap kekeringan yang ditunjukkan melalui penurunan kebocoran elektrolit dan MDA, serta peningkatan aktivitas APX. Penelitian ini diharapkan dapat memberikan informasi ketahanan tembakau dengan pemberian pupuk nitrogen terhadap kondisi kekeringan.
Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.) Maryanto, Sigit Dwi; Rohman, Randi Abdur; Roberdi, Roberdi; Nuringtyas, Tri Rini; Purnomo, Purnomo; Rachmawati, Diah; Putra, Eka Tarwaca Susila; Utomo, Condro; Liwang, Tony; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109941

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.
Seed Priming and Silicon-Based Fertilizer Application Enhanced Salinity Tolerant of Tobacco Plant (Nicotiana tabacum L.) Jannah, Na'imatul; Rachmawati, Diah
Biotropika: Journal of Tropical Biology Vol. 14 No. 2 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.02.13

Abstract

Tobacco (Nicotiana tabacum L.) is an important commodity due to its high economic value and utilisation. However, tobacco productivity is still suboptimal due to soil salinity. One effort that can be made to overcome salinity stress is to develop more tolerant tobacco varieties. To increase plant tolerance to salinity stress, seed priming and the application of silicon-based fertilisers to the growing medium can be carried out. Seed priming treatments, which control seed hydration, can increase seed germination. Silicon (Si) is a beneficial element that plays a role in increasing plant tolerance to stress. This study aims to examine the effect of seed priming and calcium silicate application on 'Jember Sili' tobacco in saline soil by observing germination, growth, and physiological responses. This Completely Randomized Design (CRD) study consisted of two factors: seed priming with calcium silicate (0 mM, 1 mM, 2 mM) and calcium silicate (CaSiO3) addition to the growing medium (0 mM, 1 mM, 2 mM). Data were analysed using SPSS for ANOVA, followed by DMRT at a 95% significance level. Tobacco (Nicotiana tabacum L. 'Jember Sili') responded positively to varying concentrations of calcium silicate priming, particularly at 1 mM, as indicated by a significant increase in germination percentage, germination rate, vigour index, Timson Germination Index (TGI), and Mean Germination Time (MGT). The 1 mM concentration was also optimal for increasing plant height, leaf number, dry weight of shoots and roots, and root length. Tobacco priming and calcium silicate application in low-saline soil also increased chlorophyll, carotenoid, and SOD levels. But it reduces H2O2 levels and MDA levels.
Co-Authors Ardadi, Maharani Pratiwi Salsabila BUDI SETIADI DARYONO Eka Tarwaca Susila Putra Iqlima, Selesa Jannah, Na'imatul Jasmine, Nataya Annisa Kumala Dewi Majidah, Salma Nur Maryani Maryani Masruroh, dan Ulfatul Milasari, Asri Fajar Mona Monika, Ni Luh Gde Nilamsari, Elvian Indah Nining Lestari Novanursandy, Nikita Besty Purnomo Purnomo Putra, Sidiq Permana Refli Retno Sintowati Rima Vegi Santika Roberdi, Roberdi Rohman, Randi Abdur Sigit Dwi Maryanto Sukarti Muljopawiro, Sukarti Tony Liwang Tri Agustina, Tri Tri Rini Nuringtyas Tri Setyaningsih Utomo, Condro Wibowo, Wiko Arif