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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Biotechnology Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Prosiding University Research Colloquium
DIAH RACHMAWATI
Staf Pengajar Fakultas Biologi UGM
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Physiological responses and production of Gama Melon Parfum (Cucumis melo L. cv. GMP) on different water availability Putri Renata, Nellis Nadinda; Daryono, Budi Setiadi; Wibowo, Wiko Arif; Rachmawati, Diah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 3 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i3.54022

Abstract

Gama Melon Parfum (GMP) cultivar is a result of crossbreeding between the Natsuno Omoide and Miyamauri melon varieties in 2011. GMP exhibits a unique phenotypic characteristic, including a bitter taste of the fruit flesh and produces a stronger aroma. The objective of this study was to investigate the physiological responses and productivity of GMP under varying water conditions. The experiment was conducted using a single factor consisting of different levels of water availability with field capacity at 50%, 75%, and 100%, as well as submergence at 2 cm, 4 cm, and 8 cm above the soil surface. Each treatment was replicated three times. The plot size for each replication was 2 m x 2 m, ensuring consistent conditions for all treatments. The results of the study showed that treatment with 100% field capacity increased stem diameter, while treatment with 50% field capacity increased the root-to-shoot ratio of GMP. Submergence treatment at 8 cm decreased stem diameter and the root-to-shoot ratio of GMP. The 50% field capacity treatment reduced the total chlorophyll levels in GMP leaves. Submergence treatment at 8 cm increased the total chlorophyll levels in GMP leaves. The 50% field capacity treatment increased fruit fresh weight, while submergence treatment at 2 cm decreased fruit fresh weight. Submergence treatment at 2 cm reduced fruit water content, whereas submergence treatment at 8 cm increased water content in GMP fruits. Keywords: drought; Gama Melon Parfume (GMP); growth; plant physiological responses; submergence
Silicon priming enhances growth and photosynthetic pigments in rice plants under drought stress Hayya, Nina Fadia; Rachmawati, Diah
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 1 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i1.60026

Abstract

Rice (Oryza sativa L.) variety 'Inpari 24 Gabusan' offers high nutritional value and a short growth cycle that is ideal for further development. This study aimed to assess the effect of silicon priming on the growth and photosynthetic pigments of rice 'Inpari 24 Gabusan' during the vegetative stage under drought conditions. A completely randomized design (CRD) was used with two factors: sodium metasilicate (Na₂SiO₃) concentrations (0 mM, 20 mM, 40 mM, and 60 mM) and field water capacity (100, 75, and 50%). Germination parameters (percentage and rate of germination, and seed vigor index) and vegetative parameters (plant height, leaf number, root length, biomass) were measured. Leaf chlorophyll and carotenoid content were also assessed. Results showed that silicon priming increased the germination rate from 57.17% at 0 mM to 63.83% at 60 mM. Seed vigor index significantly improved at 60 mM. However, sodium metasilicate concentration had no significant effect on the percentage of germination. Priming at 40 mM and 60 mM significantly enhanced growth and chlorophyll content, particularly at 100% and 75% field capacity. Under 50% field capacity, growth improvements were more limited due to water deficit. Higher sodium metasilicate concentrations also enhanced chlorophyll content, improved photosynthetic efficiency and drought tolerance. Keywords: carotenoid; chlorophyll; field capacity; germination; sodium metasilicate
Pengaruh Aplikasi Pupuk Nitrogen terhadap Ketahanan Tembakau (Nicotiana tabacum L. ‘Manilo’) pada Cekaman Kekeringan Ardadi, Maharani Pratiwi Salsabila; Nurbaiti, Siti; Rachmawati, Diah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.11663

Abstract

Tembakau (Nicotiana tabacum L.) merupakan salah satu tanaman unggulan yang berkontribusi dalam perekonomian Indonesia. Namun, perubahan iklim dapat memicu berbagai cekaman abiotik, seperti kekeringan yang dapat menghambat pertumbuhan tanaman. Salah satu upaya yang dapat dilakukan untuk meningkatkan toleransi tanaman terhadap kekeringan adalah melalui pemberian pupuk nitrogen. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian pupuk nitrogen terhadap ketahanan tembakau (Nicotiana tabacum L. ‘Manilo’) pada cekaman kekeringan. Penelitian dilakukan menggunakan metode Rancangan Blok Acak Lengkap dengan perlakuan kombinasi tiga tingkat ketersediaan air (100%, 75%, dan 50% kapasitas lapang) serta tiga dosis pupuk nitrogen (0,6; 1,2; dan 1,8 g/polybag). Parameter yang diamati meliputi respons tingkat kerusakan serta aktivitas antioksidan enzimatik. Analisis data dilakukan menggunakan ANOVA dan uji DMRT pada taraf kepercayaan 95%. Hasil menunjukkan aplikasi pupuk nitrogen mampu meningkatkan aktivitas enzim antioksidan, terutama APX. Peningkatan dosis pupuk nitrogen berkorelasi positif dengan ketahanan tanaman terhadap kekeringan yang ditunjukkan melalui penurunan kebocoran elektrolit dan MDA, serta peningkatan aktivitas APX. Penelitian ini diharapkan dapat memberikan informasi ketahanan tembakau dengan pemberian pupuk nitrogen terhadap kondisi kekeringan.
Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.) Maryanto, Sigit Dwi; Rohman, Randi Abdur; Roberdi, Roberdi; Nuringtyas, Tri Rini; Purnomo, Purnomo; Rachmawati, Diah; Putra, Eka Tarwaca Susila; Utomo, Condro; Liwang, Tony; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109941

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.
Co-Authors Ardadi, Maharani Pratiwi Salsabila BUDI SETIADI DARYONO Eka Tarwaca Susila Putra Hayya, Nina Fadia Iqlima, Selesa Jasmine, Nataya Annisa Kumala Dewi Lestari, Nining Majidah, Salma Nur Maryani Maryani Masruroh, dan Ulfatul Milasari, Asri Fajar Mona Monika, Ni Luh Gde Nilamsari, Elvian Indah Novanursandy, Nikita Besty Purnomo Purnomo Putra, Sidiq Permana Putri Renata, Nellis Nadinda Refli Retno Sintowati - Rima Vegi Santika Roberdi, Roberdi Rohman, Randi Abdur Sigit Dwi Maryanto Siti Nurbaiti Sukarti Muljopawiro, Sukarti Tony Liwang Tri Agustina, Tri Tri Rini Nuringtyas Tri Setyaningsih Utomo, Condro Wibowo, Wiko Arif