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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vegetalika Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Biotechnology Caraka Tani: Journal of Sustainable Agriculture Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Prosiding University Research Colloquium
DIAH RACHMAWATI
Staf Pengajar Fakultas Biologi UGM
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Pengaruh Aplikasi Pupuk Nitrogen terhadap Ketahanan Tembakau (Nicotiana tabacum L. ‘Manilo’) pada Cekaman Kekeringan Ardadi, Maharani Pratiwi Salsabila; Nurbaiti, Siti; Rachmawati, Diah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.11663

Abstract

Tembakau (Nicotiana tabacum L.) merupakan salah satu tanaman unggulan yang berkontribusi dalam perekonomian Indonesia. Namun, perubahan iklim dapat memicu berbagai cekaman abiotik, seperti kekeringan yang dapat menghambat pertumbuhan tanaman. Salah satu upaya yang dapat dilakukan untuk meningkatkan toleransi tanaman terhadap kekeringan adalah melalui pemberian pupuk nitrogen. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian pupuk nitrogen terhadap ketahanan tembakau (Nicotiana tabacum L. ‘Manilo’) pada cekaman kekeringan. Penelitian dilakukan menggunakan metode Rancangan Blok Acak Lengkap dengan perlakuan kombinasi tiga tingkat ketersediaan air (100%, 75%, dan 50% kapasitas lapang) serta tiga dosis pupuk nitrogen (0,6; 1,2; dan 1,8 g/polybag). Parameter yang diamati meliputi respons tingkat kerusakan serta aktivitas antioksidan enzimatik. Analisis data dilakukan menggunakan ANOVA dan uji DMRT pada taraf kepercayaan 95%. Hasil menunjukkan aplikasi pupuk nitrogen mampu meningkatkan aktivitas enzim antioksidan, terutama APX. Peningkatan dosis pupuk nitrogen berkorelasi positif dengan ketahanan tanaman terhadap kekeringan yang ditunjukkan melalui penurunan kebocoran elektrolit dan MDA, serta peningkatan aktivitas APX. Penelitian ini diharapkan dapat memberikan informasi ketahanan tembakau dengan pemberian pupuk nitrogen terhadap kondisi kekeringan.
Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.) Maryanto, Sigit Dwi; Rohman, Randi Abdur; Roberdi, Roberdi; Nuringtyas, Tri Rini; Purnomo, Purnomo; Rachmawati, Diah; Putra, Eka Tarwaca Susila; Utomo, Condro; Liwang, Tony; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.109941

Abstract

Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be quantified. Acid phosphatase is believed to be induced by P‐deprivation. Conversely, the Pto‐interaction (Pti) serine/threonine kinase activity is associated with abiotic stress. The aim of this study was to quantify of activities of two selected enzymes and phytohormone content in oil palm‐clones in the P‐limitation condition. Two oil palms genotypes were treated with three P dosages i.e. 0% (v/v), 4.67% (v/v), and 14.02% (v/v) represented as starvation, deficiency, and optimum condition, respectively. The activity of these two enzymes was quantified in mitochondria and cytoplasm using spectrophotometry and modified dot‐blot methods, while abscisic acid, indole acetic acid and gibberellic acid content was quantified using ultra performance liquid chromatography (UPLC). The result showed that the Apase activity in P‐optimum was higher than starvation and deficiency in leaf and root tissues in both genotypes, whereas Pti serine/threonine kinase activity was higher in prolific than non‐prolific genotypes in P‐deficient dosage. Furthermore, abscisic acid content was higher in prolific than non‐prolific genotypes in starvation and deficient, whereas other hormone contents were similar. Association study showed that prolific was separated with non‐prolific ones at different doses of P. Finally, the prolific genotype is more adaptable with P deficiency.
Calcium Silicate Application Enhances Oxidative Defense and Improves the Physiological and Growth Responses of Shallot (Allium cepa L. Aggregatum Group) Under Salinity Stress Putra, Sidiq Permana; Sukirno, Sukirno; Nugroho, Laurentius Hartanto; Rachmawati, Diah
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108192

Abstract

Indonesia’s shallot production still falls short of domestic demand, necessitating imports. Expanding cultivation into marginal coastal areas such as Yogyakarta is promising but constrained by soil salinity. Silicon can help by enhancing plant resistance to such abiotic stress. This study evaluated the physiological and biochemical responses of shallot plants (Allium cepa L. Aggregatum group) to the application of calcium silicate (CaSiO3) under saline conditions. The experiment employed a completely randomized design with 2 factors: CaSiO3 (0, 2, and 4 mM) and salinity (0, 2, 4, and 8 dS m⁻¹), each with 5 replications. Physiological parameters, antioxidant activity, and yield traits were analyzed using analysis of variance (ANOVA) followed by Duncan multiple range test (DMRT) at p ≤ 0.05. CaSiO3 significantly alleviated salt stress by enhancing superoxide dismutase (SOD) activity and membrane stability, improving photosynthetic efficiency, promoting growth, and yield components. Under high salinity, 4 mM CaSiO3 reduced proline and H₂O₂ accumulation compared with untreated plants. These findings indicate that applying 4 mM CaSiO3 can enhance shallot productivity and resilience in saline coastal soils, supporting sustainable shallot self-sufficiency in Indonesia.
Co-Authors Ardadi, Maharani Pratiwi Salsabila BUDI SETIADI DARYONO Eka Tarwaca Susila Putra Iqlima, Selesa Jasmine, Nataya Annisa Kumala Dewi L. Hartanto Nugroho Majidah, Salma Nur Maryani Maryani Masruroh, dan Ulfatul Milasari, Asri Fajar Mona Monika, Ni Luh Gde Nilamsari, Elvian Indah Nining Lestari Novanursandy, Nikita Besty Purnomo Purnomo Putra, Sidiq Permana Refli Retno Sintowati Rima Vegi Santika Roberdi, Roberdi Rohman, Randi Abdur Sigit Dwi Maryanto Siti Nurbaiti Sukarti Muljopawiro, Sukarti Sukirno Sukirno Tony Liwang Tri Agustina, Tri Tri Rini Nuringtyas Tri Setyaningsih Utomo, Condro Wibowo, Wiko Arif