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All Journal Buletin Peternakan VALENSI Jurnal Lahan Suboptimal AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Sumberdaya Hayati Livestock and Animal Research Jurnal Biodjati Jurnal Sains Teh dan Kina Jurnal Kimia Terapan Indonesia Jurnal Mikologi Indonesia Indonesian Aquaculture Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Warta AKAB Makara Journal of Science

Nani Radiastuti

Program Studi Biologi, FST Universitas Islam Negeri Syarif Hidayatullah, Jakarta

Author-ID : 354148

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Veterinary Other

Published : 28 Documents Claim Missing Document

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Aktivitas Antibakteri Kapang Endofit dari Tanaman Kina (Cinchona calisaya Wedd.) Zakiyah, Alfida; Radiastuti, Nani; Sumarlin, La Ode
Al-Kauniyah: Jurnal Biologi Vol 8, No 2 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Endophytic microorganisms are microorganisms that live in the tissues of plant organ and not harm its host. One of them is the endophytic fungi. Endophytic fungi could produce the same compound as the host plant. The Plants produced alkaloids quinine that could potentially inhibit the bacteria Staphylococcus aureus and Escherichia coli. This study aims to test the potential of endophytic fungi produced quinine as an antibacterial. The method used to test the antibacterial is a paper disc diffusion. Results of the analysis of data using one-way ANOVA showed that there are significant differences zona between the diametre of inhibitory of the endophytic fungi extract. Endophytic fungi M16 and M33 produced the greatest inhibition zone against Staphylococcus aureus and Escherichia coli 14.9 mm and 9.2 mm respectively.

Efek Toksisitas Subakut Serbuk Biji Pepaya (Carica papaya) Varietas ‘Bangkok’ dan ‘California’ Pada Mencit Jantan (Mus musculus) Galur Swiss Webster Nani Radiastuti; Indri Garnasih; Sena Yunia Saputri
Al-Kauniyah: Jurnal Biologi Vol 14, No 2 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i2.16039

AbstrakBiji pepaya memiliki rasa yang pahit, pedas, dan beraroma menyengat sehingga kurang diminati untuk diolah. Bahan tersebut belum banyak dimanfaatkan secara optimal, padahal potensinya sangat besar untuk dijadikan sebagai bahan pengawet makanan. Oleh karena itu, perlu dilakukan penelitian untuk menguji keamanannya. Penelitian ini bertujuan untuk membandingkan efek toksisitas subakut serbuk biji pepaya ‘Bangkok’ dan ‘California’ secara in vivo pada mencit jantan galur Swiss Webster. Penelitian dilakukan dengan metode eksperimen, terdiri dari empat perlakuan serbuk biji pepaya yang dilarutkan dengan CMC 1% (dosis 0, 400, 600, dan 800 mg/kg BB). Semua dosis diberikan pada mencit secara gavage dengan volume 0,1 mL/10 g BB. Perlakuan diberikan setiap hari selama 28 hari. Pada hari ke-29, darah mencit diambil melalui retroorbital untuk diuji SGPT dan SGOT. Perlakuan dosis serbuk biji pepaya tidak berpengaruh terhadap berat badan, kondisi fisik, dan organ visceral mencit, namun meningkatkan kadar SGPT dan SGOT. Peningkatan tersebut masih di bawah ambang batas normal SGOT (70–400 U/L) dan SGPT (25–200 U/L).Abstract Papaya seeds have a bitter, spicy, and pungent aroma so they are less desirable for processing. The material has not been used optimally, even though its potential is to be used as a food preservative. Therefore, research is needed to examine its safety. This study aimed to compare the effect of subacute toxicity of 'Bangkok' and 'California' papaya seed powder in vivo on male mice of Swiss Webster strain. The research was conducted using an experimental method, consisting of four treatments of papaya seed powder dissolved in 1% CMC (dose 0, 400, 600, and 800 mg/kg BW). All doses were given to mice by gavage with a volume of 0.1 mL/10 g BW. The treatment was given every day for 28 days. On day 29, the blood of mice was taken retroorbitally to be tested for SGPT and SGOT. The dose of papaya seed powder treatment showed no effect on body weight, physical condition, and visceral organs of mice, but increased levels of SGPT and SGOT. The increase was still below the normal threshold for SGOT (70–400 U/L) and SGPT (25–200 U/L).

Uji Aktivitas Antibakteri Ekstrak Air Bunga Kecombrang terhadap Bakteri E. coli dan S. aureus sebagai Bahan Pangan Fungsional Adeng Hudaya; Nani Radiastuti; Dede Sukandar; Ira Djajanegara
Al-Kauniyah: Jurnal Biologi Vol 7, No 1 (2014): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The testing has been conducted research on the antibacterial activity of aqueous extracts of flowers kecombrang against E. coli and S. Aureus. This study was aims to provided scientific evidence of excellence kecombrang plants as functional food ingredients. Antibacterial activity assays performed using dispersive method. From the results of antibacterial testing kecombrang flower water extract against E. coli concentration of 20% = 0 mm, 40% = 0 mm, 60% = 4.8 mm, 80% = 5.2 mm, 100% = 7.3 mm and the test bacteria S. aureus concentration of 20% = 8.67 mm, 40% = 9.11 mm, 60% = 12:33 mm, 80% = 12:44 mm, 100% = 13.89 mm.

Aplikasi Effective Microorganism 10 (Em10) untuk Pertumbuhan Ikan Lele Sangkuriang (Clarias gariepinus var. Sangkuriang) di Kolam Budidaya Lele Jombang, Tangerang Elpawati Elpawati; Dianna Rossyta Pratiwi; Nani Radiastuti
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

The catfish is a fish that is widely cultivated and consumed in Indonesia. The Catfish growth depand on by the availability of food and water quality. EM4 and EM10 are an example of liquid biofertilizer. The addition of EM4 in the fish pond can help the growth of the fish and maintain of the water quality. While the test of EM10 has not been done on fishery fields. The purpose of this study was to determine the effect of EM10 fertilizers on of sangkuriang catfish (Clarias gariepinus Var. sangkuriang) growth and water quality. This research was conducted in February-March 2014. Research using completely randomized design with 7 treatments and 3 replications. The treatments tested were control (A), EM4 10 ml (B), EM4 20 ml (C), EM4 30 ml (D), EM10 10 ml (E), EM10 20 ml (F), and EM10 30 ml (G ). Analysis of data were using ANOVA followed by Duncan test if there is a real effect (ᾱ = 0.05). EM10 fertilizers at concentration of 20 ml could affect to the specific growth rate on catfish in 7 days maintenance, the concentration of 10 ml at 14 days of villa maintenance and the concentration of 30 ml at 28 days of maintenance. EM10 fertilizers can affect the weight growth of catfish. Fertilizer EM10 can maintain the temperature of the water.

Isolation And Characterization of Bacteria from Shallots (Allium cepa L.) as In-vitro Biocontrol Agent of Fusarium oxysporum f.sp cepae Radiastuti, Nani; Amalia, Wuliani; Suryadi, Yadi; Susilowati, Dwi N; Kosasih, Jajang
Al-Kauniyah: Jurnal Biologi Vol. 17 No. 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i2.38132

AbstractShallot is one of the leading vegetable commodities with many benefits such as for seasonings and herbal medicinal ingredients. The demand for shallots continues to increase; however, shallot production is still relatively low. One of the limiting factors causing low shallot production is due to wilt disease caused by Fusarium oxysporum f.sp cepae (Foc). Bacteria have many roles in suppressing the growth of Foc, and this study aims to obtain potential bacterial isolates from the shallot plant to inhibit the growth of Foc Based on fungal diameter zone inhibition, degree of inhibition, and chitinase test, it was obtained 9 isolates which could suppress the growth of Foc. The results indicated that the AB3, TB2, and UB1 bacterial isolates could inhibit the growth of Foc with a percentage of inhibition of 46.80; 40.24; and 35.11%, respectively. The analysis showed that AB3, TB2, and UB1 isolates were categorized as moderate in suppressing the growth of Foc. The 16S rRNA sequencing results showed that AB3 and TB2 isolate had similarities with Bacillus subtilis by 99,75%, and 100%, respectively, while UB1 isolate had similarities with Pseudomonas nitroreducens by 89,35%. Based on the result showed that Bacillus sp. AB3 and TB2 isolates, and P. nitroreducens UB1 isolate have more potential as biological control agents to control the Fusarium wilt at in vitro assay. The field efficacy studies on these potential antagonists need to be done in the future.AbstrakSalah satu faktor pembatas yang menyebabkan rendahnya produksi bawang merah adalah penyakit layu yang disebabkan oleh Fusarium oxysporum f.sp cepae (Foc). Bakteri antagonis memiliki banyak peran dalam menekan pertumbuhan Foc, dan penelitian ini bertujuan untuk mendapatkan isolat bakteri antagonis potensial asal tanaman bawang merah untuk menghambat pertumbuhan Foc. Berdasarkan nilai zona hambat diameter jamur, derajat hambat dan uji kitinase, diperoleh 9 isolat yang dapat menekan pertumbuhan Foc. Hasil penelitian menunjukkan bahwa isolat bakteri AB3, TB2, dan UB1 mampu menghambat pertumbuhan Foc dengan persentase penghambatan masing-masing sebesar 46,80; 40,24; dan 35,11% dengan kategori penghambatan pertumbuhan Foc moderat. Hasil sekuensing 16S rRNA, menunjukkan bahwa isolat AB3 dan TB2 memiliki kemiripan dengan Bacillus subtilis masing-masing sebesar 99,75%, dan 100%, sedangkan isolat UB1 memiliki kemiripan dengan Pseudomonas nitroreducens sebesar 89,35%. Berdasarkan hasil penelitian menunjukkan bahwa Bacillus sp. isolat AB3, TB2, dan P.nitroreducens isolat UB1 berpotensi digunakan sebagai agen pengendali hayati untuk mengendalikan penyakit layu Fusarium pada uji in vitro. Studi kemanjuran lapangan terhadap isolat antagonis potensial ini perlu dilakukan di masa depan.

In Vivo Testing of Pseudozyma hubeiensis to Control Aspergillus flavus in Nutmeg Seeds (Myristica fragrans Houtt.) Rahayuningsih, Sri; Hidayah, Mudrikah Nurul; Susilowati, Dwi Ningsih; Supriadi; Suryadi, Yadi; Rismayani; Radiastuti, Nani
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.46462

Nutmeg (Myristica fragrans Houtt.) is a high-value spice commodity from Indonesia, often contamination with aflatoxin-producing Aspergillus flavus. Previous research has identified two epiphytic yeast isolates from nutmeg plants, namely DP1341a and DP1342, both of which were identified as Pseudozyma hubeiensis and showed antagonistic potential against A. flavus. This study is a follow- up study that aims to test the antagonistic activity of both isolates against A. flavus in vivo on nutmeg seeds. The research was conducted experimentally through several stages, namely testing the compatibility of isolates on Yeast Malt Agar (YMA) media, determining the optimum fermentation time, and applying liquid culture and fermentation filtrate to 120 nutmeg seeds. Results showed that the consortium of isolates DP1341a and DP1342 did not increase the inhibitory power results compared to single cultures with optimum fermentation times of 10 and 14 days, respectively. In vivo applications show that treatment with DP1342 isolate (KC-DP1342) and consortium in liquid culture provides the highest level of resistance to A. flavus infection, namely 38.67% and 28.00%. The findings suggest that P. hubeiensis DP1342 has potential as a biological control agent in controlling A. flavus on nutmeg seeds, although further formulation and optimization are needed.

Co-Authors A Sa’duddin Achmad Dinoto Adeng Hudaya Aina Nadila Alfida Zakiyah, Alfida Amalia, Wuliani Amelia Rakhmaniar Anna Muawanah Berty, Gusniar Dede Sukandar Dianna Rossyta Pratiwi Dwi Agustyani Dwi N Susilowati Dwi N Susilowati Dwi Ningsih Susilowati DWI NINGSIH SUSILOWATI Dwikencana, Windy Elpawati, Elpawati Endang Tri Margawati Endang Tri Margawati F Wijayanti Firdaus Ramadhan Fitri, Reno Gayuh Rahayu Gusniar Berty Hajar Indra Wardhana Heddy Julistiono Hidayah, Mudrikah Nurul Ika Roostika Ika Roostika Tambunan Iman Hidayat Indah Sofiana Indri Garnasih Ira Djajanegara Ira Djajanegara Ira Jayanegara Izumi Okane, Izumi Khoeriyah Khoeriyah Kosasih, Jajang La Ode Sumarlin Lisca Puji Rustanti Maddona, Sandra Muhammad Ridhwan Muhammad, Alfianur Azmi Mulyadi Mulyadi Ninu Setianingrum Nurhasni, N. Qoriatul Ilma R Fathoni Rahayu, Rizkiyah Putri Reno Fitri Rina Ningtiyas Rismayani Saiful Bahri Sena Yunia Saputri Setiyani, Alfi Dwi Slamet Diah Volkandari Slamet Diah Volkandari Sofiana, Indah Solihat, Nur Amaliah Sri Rahayuningsih Sri Rahayuningsih Suminar S. Achmadi Sunandar, Ibnu Hasan Supriadi Suryadi, Yadi Susilowati, Dwi Ningsih Tentrem, Titi Yadi Suryadi

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