Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

1.77

P-Index

This Author published in this journals

All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Microbiology Indonesia agriTECH JURNAL MANAJEMEN TRANSPORTASI & LOGISTIK Industria: Jurnal Teknologi dan Manajemen Agroindustri Animal Production : Indonesian Journal of Animal Production Jurnal Gizi Klinik Indonesia Indonesian Food and Nutrition Progress Indonesian Food Science and TechnologyJournal Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Manajemen Transportasi & Logistik (JMTRANSLOG)

Winiati Pudji Rahayu

Department Of Food Science And Technology, Faculty Of Agricultural Technology, Bogor Agricultural University, Campus IPB Dramaga, PO Box 220 Bogor 16680

Author-ID : 355132

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Public Health Transportation Veterinary Other

Published : 41 Documents Claim Missing Document

Claim Missing Document

Articles

Title

Pengaruh Suhu dan Kelembaban terhadap Pertumbuhan Fusarium verticillioides Bio 957 dan Produksi Fumonisin B1 Dwi Rahayu; Winiati Pudji Rahayu; Hanifah Nuryani Jenie; Dian Herawati; Wisnu Broto; Santi Ambarwati
agriTECH Vol 35, No 2 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Fusarium verticillioides was the predominant species in producing fumonisin on agricultural products. Fumonisisn B1 (FB1) is the most abundant fumonisin in nature and the most toxic than other fumonisin. The main factors affecting the growth of and production of fumonisin are temperature and humidity. This research aimed to assess the effect of changes in temperature and humidity on the growth of and FB1 production on maize and soybeans medium. Maize and soybeans that have inoculated with suspension Bio 957 were incubated at 20, 30 and 40 °C with 70, 80 and 90% of humidity for 14 days. Observations of growth made by weighing the cells mass and analysis of FB1 production performed by HPLC. The results showed that the highest growth of Bio 957 in maize and soybeans was occurred at temperature 30 °C and 90% of humidity, the cell mass weights were 904,5 and 885,5 mg per 20 g of maize and soybeans respectively. The highest concentration of FB1 in maize and soybeans were 374 and 67 pbb respectively, observed at temperature 30 °C for maize and 20 °C for soybeans, both at same humidity (90%). The results showed that Bio 957 was able to grow well and produced the highest concentrations of FB1 in maize and soybeans at a temperature of 20 and 30 °C with 90% of humidity. At a temperature of 40 °C with 70, 80 and 90% of humidity, the growth of was not observed, therefore FB1 formation was avoided.ABSTRAKFusarium verticillioides adalah spesies yang dominan dalam memproduksi fumonisin pada produk-produk pertanian. Fumonisin B1 (FB1) merupakan fumonisin yang paling banyak ditemukan di alam dan paling toksik dibandingkan jenis fumonisin lainnya. Faktor ekstrinsik utama yang mempengaruhi pertumbuhan dan produksi FB1 adalah suhu dan kelembaban. Penelitian ini bertujuan untuk mengkaji pengaruh suhu dan kelembaban terhadap pertumbuhan Bio 957 dan produksi FB1 pada media jagung dan kedelai. Jagung dan kedelai yang telah diiinokulasi dengan suspensi Bio 957 diinkubasi pada suhu 20, 30 dan 40 °C dengan kelembaban 70, 80 dan 90% selama 14 hari. Pengamatan pertumbuhan dilakukan dengan penimbangan massa sel dan analisis konsentrasi FB1 dilakukan dengan HPLC. Hasil penelitian menunjukkan bahwa pertumbuhan Bio 957 pada jagung dan kedelai paling tinggi terjadi pada suhu 30 °C dan kelembaban 90%, berat massa selnya yaitu 904,5 dan 885,5 mg per 20 g masing-masing jagung dan kedelai. Konsentrasi FB1 paling tinggi pada jagung dan kedelai masing-masing yaitu 374 dan 67 ppb, pengamatan pada suhu 30 °C pada jagung dan 20 °C pada kedelai, keduanya pada kelembaban yang sama (90%). Hasil penelitian menunjukkan bahwa Bio 957 mampu tumbuh dengan baik dan menghasilkan konsentrasi FB1 paling tinggi pada jagung dan kedelai pada suhu 20 dan 30 °C dengan kelembaban 90%. Pada suhu 40 °C dengan kelembaban 70, 80 dan 90%, Bio 957 tidak menunjukkan adanya pertumbuhan, sehingga pembentukan FB1 dapat dihindari.

Aktivitas Antiaflatoksin B1 Ekstrak Daun Rumput Kebar (Biophytum petersianum) terhadap Aspergillus ﬂavus Meike Meilan Lisangan; Rizal Syarie; Winiati Pudji Rahayu; Okky Setyawati Dharmaputra
agriTECH Vol 35, No 1 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Aﬂatoxin B1 was a secondary metabolite produced by Aspergillus ﬂavus having negative effect on human health because of its carcinogenic. Many efforts have been done to investigate the antifungal and antiaﬂatoxin agent derived plant. The objective of this research was to study the activity of antifungal from kebar grass leaf extract on mycelial growth and aﬂatoxin B1 production of Aspergillus ﬂavus BCC F0219 and A. ﬂavus BIO 2236 isolates in food model medium i.e. carbohydrate-enriched medium, fat-enriched medium and protein-enriched medium. Kebar grass leaf extracts was successively obtained by using n-hexane - ethyl acetate - methanol (HEM). Concentrations of the extract tested on A. ﬂavus BCC F0219 and A. ﬂavus BIO 2236 were 1; 1.5, and 2 MIC. The MIC for A. ﬂavus BCC F0219 in carbohydrate-enriched medium, fat-enriched medium, and protein-enriched medium were 12, 14, and 14 mg/mL, respectively. Meanwhile, the MIC for A. ﬂavus BIO 2236 in carbohydrate-enriched medium, fat-enriched medium and protein-enriched medium were 12, 16 and 16 mg/mL, respectively. The results showed that the percentage of growth inhibition of A. ﬂavus BCC F0219 and BIO 2236 in carbohydrate, fat and protein-enriched medium at 3 different levels of MIC concentrations ranged between 90.8 - 100% and 93.8 - 100%. The inhibitory effect of Aﬂatoxin B1 production of A. ﬂavus F0219 BCC and BIO 2236 in carbohydrate, fat and protein-enriched medium at 3 different levels of MIC concentration ranged between 70.86 - 100 % and 83.42 – 98.84 %.ABSTRAKAflatoksin B1 merupakan metabolit sekunder yang dihasilkan oleh Aspergillus ﬂavus yang berbahaya bagi kesehatan karena bersifat karsinogenik. Berbagai upaya telah dilakukan untuk mencari bahan antikapang dan antiaﬂatoksin yang berasal dari bahan alami seperti tumbuhan. Tujuan penelitian ini adalah mempelajari aktivitas ekstrak daun rumput kebar terhadap pertumbuhan miselium dan produksi aﬂatoksin B1 dari isolat A. ﬂavus BC F0219 dan A. ﬂavus BIO 2236 pada media model pangan kaya karbohidrat, lemak dan protein. Ekstrak daun rumput kebar diekstraksi secara bertingkat dengan pelarut n-heksana-etil asetat-metanol (HEM). Konsentrasi ekstrak yang diuji untuk isolatA. ﬂavus BCC F0219 dan A. ﬂavus BIO 2236 masing-masing adalah 1; 1,5; dan 2 MIC (Minimum Inhibitory Concentration). Nilai MIC untuk A. ﬂavus BCC F0219 pada media kaya karbohidrat, lemak, dan protein berturut-turut sebesar 12, 14, dan 14 mg/mL. Sedangkan nilai MIC untuk A. ﬂavus BIO 2236 pada media kaya karbohidrat, lemak, dan protein berturutturut sebesar 12, 16, dan 16 mg/mL. Hasil pengujian memperlihatkan bahwa persentase hambatan pertumbuhan isolat A. ﬂavus BCC F0219 dan BIO 2236 pada media kaya karbohidrat, lemak dan protein pada 3 tingkat konsentrasi MIC berkisar antara 90,8 – 100% dan 93,8 – 100%. Hambatan produksi aﬂatoksin B1 isolat A. ﬂavus BCC F0219 dan BIO 2236pada media kaya karbohidrat, lemak dan protein pada 3 tingkat konsentrasi MIC berkisar antara 70,86 – 100% dan 83,42 – 98,84%.

Aktivitas Antimikroba Minyak Esensial Jahe Merah (Zingiber ofﬁcinale var. Rubrum) dan Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Bakteri Patogen dan Perusak Pangan Tita Rialita; Winiati Pudji Rahayu; Lilis Nuraida; Budi Nurtama
agriTECH Vol 35, No 1 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B. cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, speciﬁc gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, speciﬁc gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol, -caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm. Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values, all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry. ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristik ﬁsika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC). Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik -9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambat pertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.

Fungal Population of Nutmeg (Myristica fragrans) Kernels Affected by Water Activity During Storage Kiki Nurtjahja; Okky Setyawati Dharmaputra; Winiati Pudji Rahayu; Rizal Syarief
agriTECH Vol 37, No 3 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The aim of this investigation was to determine the effect of various water activities (aw) on fungal population in nutmeg kernels during storage. The seed nutmegs were obtained from ripe fruits one week after they fell on the ground in North Minahasa Regency, North Sulawesi Province, Indonesia. The kernels (moisture content ± 10%) were stored 0, 15, and 30 days in various aw (0.75, 0.80, 0.83, 0.90, 0.97) using saturated salt solutions at 29 °C in sorption containers. Serial dilution method followed by a pour-plate method in Dichloran 18% Glycerol Agar (DG18) was used to isolate and quantify the fungal population. Results revealed that kernels stored at aw = 0.75 was not significantly (p < 0.05) different from at aw = 0.80-0.83. Fungal population of kernels determined aw and significantly (p < 0.05) influenced by duration of storage. Range of aw 0.80-0.83 has a smaller total fungal population than aw ≥ 0.90. Thirteen different genera/species were isolated and identified including Aspergillus and Eurotium (6 species), Penicillium (3 species), Fusarium (2 species), 1 species each of Cladosporium or Syncephalastrum, and isolate A. The largest total fungal population (5.0×105 CFU g-1) was present at the beginning of storage (aw = 0.97) and it was dominated by Penicillium citrinum (2.6×105 CFU g-1) followed by Cladosporium cladosporioides (1.7×105 CFU g-1). After 30 days of storage (aw = 0.97) the population of P. citrinum was still dominant with a population of 2.4×104 CFU g-1. Eurotium chevalieri followed with a population of 1.2×104 CFU g-1.

Co-Authors Afrilia Sandra Ramadhani Agesti, Puja Amiroh Amiroh Amiroh Amiroh, Amiroh Andro Frando Situmorang Anjani Anggitasari Arie Norman Riandi Bangun Raharjo Betty Sri Laksmi Jenie Budi Nurtama C Hanny Wijaya Dase Hunaefi Dede Robiatul Adawiyah Dharmawan, I P G Arya Dian Herawati Dr. Adolf J.N. Parhusip Dwi Rahayu Dyah Sista Raharjanti Eko Hari Purnomo Ervizal AMZU Feri Kusnandar Galih Nugroho Gerardus YosuaYosua Gita, Sophia Ekaristi Dharma Hanifah Nuryani Jenie Harsi D. Kusumaningrum I P G Arya Dharmawan Iftita Rakhma Ikrima Jian Septian Katarina Maharani KIKI NURTJAHJA Lilis Nuraida MADE ASTAWAN Meike Meilan Lisangan Nanda, Riska Dwi Ni Made Vina Citanirmala Nugraha Edhi Suyatma Nugroho, Galih Nuri Andarwulan Nurwitri, Caecillia Chrismi Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Oryssa Sathalica Pradianti Palupi, Nurheni Sri Pipi Puspita Sari Puspa Mega Hayati Puspo Edi Giriwono Putri, Permata Adinda Qonitatin Wafiyah Ratih Dewanti -Hariyadi Ratih Paramastuti Rizal Syarie Rizal Syarief Ruki Fanaike Santi Ambarwati SEDARNAWATI YASNI Septian, Jian Siti Nurjanah Siti Nurjanah Siti Nurulfalah Situmorang, Andro Frando Sophia Ekaristi Dharma Gita Tasya Fitri Yunada Taufikkilah Romadhon Tita Rialita Tjahja Muhandri Triana Setiawardani, Triana Uswatun Hasanah Wibisono Adhi Wibisono Adhi, Wibisono Wibisono Wibisono Wibisono Wibisono Widiati Purnawita WIDYA EKA PRAYITNO Wisnu Broto Wiwit Arif Wijaya Yanti Ratnasari yermia, yermia yermia Yosua, Gerardus

Title Search

Found 4 Documents Search Journal : agriTECH

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search
Journal : agriTECH