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Antibacterial Activity Test of Indigenous Yeast from Sapodilla Fruit against Staphylococcus aureus and Escherichia coli GEMILANG LARA UTAMA; MUTIARA NABILA; HENI RADIANI ARIFIN; ELAZMANAWATI LEMBONG; TITA RIALITA
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1038.761 KB) | DOI: 10.5454/mi.13.4.1

Abstract

The research aimed to identify indigenous yeast antibacterial activity from sapodilla fruit against Escherichia coli and Staphylococcus aureus, which conducted by experimental methods and followed by descriptive analysis. This study was done by the isolation of indigenous yeast, macroscopic and microscopic identification, yeast identification using RapID Yeast Plus System, antibacterial test by measuring the clear zone diameter, testing of pathogenic bacteria viability against indigenous yeast and identification of organic acid produced by yeast. The results of yeast isolation obtained 1 isolate (Saccharomyces cereviseae 1) from fruit and 3 isolates form sapodilla skin (S.cereviseae 2, Candida famata, and Pichia anomala) which had antibacterial activity against E. coli and S. aureus except C. famata isolates. Isolates with the largest antibacterial activity against E. coli and S. aureus based on the clear zone diameter were S. cerevisiae (2) isolates. The results of organic acid analysis by HPLC found that S.cerevisiae (2) isolate produced the highest organic acid namely acetic acid as much as 2.442 mg mL -1.   Key words : antibacterial, organic acid, sapodilla fruit, yeast
The Evaluation of Spray Drying Process Condition on the Characteristics of Xylitol Powder from Oil Palm Empty Fruit Bunches Mardawati, Efri; Rialita, Tita; Suryadi, Edy; Rahmah, Devi Maulida; Anggraini, Sulistina; Bindar, Yazid
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 9, No 1 (2020)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2020.009.01.3

Abstract

AbstractXylitol is a low-calorie, natural sweetener which has similar sweetness properties to sucrose. Xylitol can be produced from the hemicellulose hydrolysate of Oil Palm Empty Fruit Bunches (OPEFB). Several steps required for this process, including pretreatment, the hydrolysis of xylan to xylose and the reduction of xylose to xylitol. In a commercial market, xylitol is commonly found in the form of powder in order to maintain its distribution stability and practicality. The spray drying method is a drying method that is mostly used in dry or powder products manufacturing industry. This research aims to investigate the effect of spray drying and to determine proper inlet temperature and maltodextrin concentration on xylitol powder characteristics that are similar to the quality of commercially produced xylitol. Randomized Group Design Method is a data processing method used in this research which consisted of two factors: inlet temperature (160 oC and 180 oC) and maltodextrin concentrations (20%, 25%, and 30%). Based on the result of the research, the inlet temperature significantly affected the water content, solubility, hygroscopic levels and calorie content. Results also showed that maltodextrin concentration had a significant effect on the hygroscopic levels, water content and calorie content. Xylitol powder similar to the commercially produced xylitol was obtained from the inlet temperature of 160 oC and 20% of maltodextrin, with the water content of 5.17%, hygroscopic levels of 8.55%, solubility of 99.346%, calorie content of 2.71 cal/g and xylitol content of 0.034 g/L.Keywords: inlet temperature, maltodextrin, oil palm empty fruit bunch, spray drying, xylitol
The effetiveness of various sequestrants on the reduction of cadmium and lead levels in Remis (Donax sp.) In-In Hanidah; Jedi Riazi; Tita Rialita; Sumanti Debby Moody; Yuli Andriani; Noor Taufiq; Annisa Ratri Utami; Laras Aprilianti
AcTion: Aceh Nutrition Journal Vol 9, No 4 (2024): December
Publisher : Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/action.v9i4.1948

Abstract

Mussels (Donax sp.) are marine shellfish that tend to be contaminated with heavy metals as they are filter feeders. Heavy metal levels can be reduced by immersion in sequestrants. This study aimed to measure the effectiveness of the optimal sequestrant to reduce heavy metal levels in mussels. This experimental study was conducted at the Food Technology Laboratory of Padjadjaran University in January-April 2024 using samples of mussels (Donax sp.) from Cirebon, Indonesia. The data processing method used was a Randomized Group Design with five treatments consisting of soaking mussels in distilled water, 25% citric acid, 25% tamarind, 25% tomato, and 25% Pidada; each repeated 3x. The results showed that the most optimal organic sequestrant in reducing heavy metals was Pidada fruit with the effectiveness of reducing cadmium metal 21,195%, lead metal 6,543%, total citric acid 0.552%, water content 76.953%, and antioxidant activity based on IC50 value of 19,537 ppm with a very strong category. In conclusion, the Pidada fruit is the most optimal organic sequestrant for reducing heavy metal levels in mussels (Donax sp.) and has very strong antioxidant activity, making it effective for reducing heavy metal contamination.
Exploration of Antifungal Tests Based on Δ-Guaiene From Subang Patchouli Oil Rahmawati, Rahmi; Nurjanah, Sarifah; Rialita, Tita; Mardawati, Efri; Filianty, Fitry
International Journal of Science and Environment (IJSE) Vol. 5 No. 3 (2025): August 2025
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijse.v5i3.194

Abstract

Guaiene is a derivative compound produced as a byproduct in the production of patchouli alcohol from patchouli oil (Pogostemon cablin Benth.). This study aims to evaluate the antifungal activity of δ-guaiene extracted from patchouli oil originating from Subang against four types of skin pathogenic fungi: Aspergillus niger, Candida albicans, Microsporum gypseum, and Trichophyton mentagrophytes. The method used was a laboratory experiment, with the main parameters observed including Inhibition Zone Diameter (IZD), Minimum Inhibitory Concentration (MIC), and Minimum Fungicidal Concentration (MFC). The results showed that δ-guaiene inhibited the growth of C. albicans at a concentration of 40%, with an IZD of 0.15 mm. Against A. niger, the compound demonstrated an inhibition zone of 1.4 mm at a 20% concentration and had an MFC of 20%. For T. mentagrophytes, an IZD of 0.4 mm was observed, with both MIC and MFC at 20%. Activity against gypseum showed an IZD of 0.25 mm, with MIC and MFC also at 20%. These findings suggest that δ-guaiene from patchouli oil has potential as a candidate antifungal agent derived from local resources, supporting the development of natural bioactive compounds and sustainability.