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Journal : Planta Tropika

Thidiazuron-Induced Somatic Embryogenesis in Cymbidium bicolor Orchid In Vitro Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.15306

Abstract

Cymbidium bicolor is a highly hunted and traded orchid, leading to a decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) is a growth regulator used to induce somatic embryogenesis. This study aimed to determine the optimal TDZ concentration for somatic embryo formation. Stem explants of C. bicolor were cultured on Murashige Skoog (MS) medium with TDZ concentrations of 0, 1, 2, and 3 ppm. Observations were conducted weekly for two months using a stereo microscope and OptiLab. Variables observed included the percentage of green explants, somatic embryo formation time, the number of explants forming somatic embryos, and the number and morphology of somatic embryos. The study was arranged in a Completely Randomized Design (CRD) with 14 replications. Results showed that TDZ addition influenced somatic embryo formation and maintained the green color of explants. Media with TDZ promoted faster growth and larger embryo size compared to media without TDZ. The optimal concentration was 1 ppm TDZ, which produced the highest number of embryos (172) and the fastest formation time compared to other concentrations (TDZ 0: 27, TDZ 2 ppm: 60, TDZ 3 ppm: 39).
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Einstivina Nuryandani Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Imam Purwanto Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Muhammad Syaiful Anam Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Noveira, Ameviana Fitri Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utama, Rafii Satria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Zahrotunnisa, Alfi Zakiyah, Aisyah