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All Journal Jurnal Biologi Udayana AL KAUNIYAH Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) BIOMA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) BIOPROSPEK Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Ilmiah Universitas Batanghari Jambi Biosaintifika: Journal of Biology & Biology Education Biosel: Biologi Science and Education JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Jurnal Riset Biologi dan Aplikasinya Infokes : Jurnal Ilmiah Rekam Medis dan Informasi Kesehatan Bioscientist : Jurnal Ilmiah Biologi JURNAL STUDIA ADMINISTRASI KESANS : International Journal of Health and Science Informatics, Electrical and Electronics Engineering Journal of Social Outreach Jurnal Natural JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat
Imam Rosadi
Department Of Biology, Faculty Mathematics And Natural Sciences, Mulawarman University
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Journal : Molecular and Cellular Biomedical Sciences (MCBS)

 1 
The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells Imam Rosadi; Karina Karina; Iis Rosliana; Siti Sobariah; Irsyah Afini; Tias Widyastuti; Anggraini Barlian
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5637.848 KB) | DOI: 10.21705/mcbs.v3i1.43

Abstract

Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment.Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC. Key words: human ADSC, PRP, L-ascorbic acid, proliferation, chondrogenesis
Co-Authors Abriyanto, Hery Adlia Khalisha Afini, Irsyah Al Hasbi, Ezra Alam, Megawati Citra Alfida Zakiyah An Nissa Falaq Qurrahmah An Nissa falaq Qurrahmah Anggraini Barlian Anggraini Barlian Arief Lelono Arum Aryani, Retno Cici Lis Qurrotun Ayuni Crisnia Crisnia Cyndi Marcelina DEMO, HILDIANA APRILIANI DHIU Dewi Elfidasari Dwi Atmi Narwati Edy Kustiani Fawaidul Badri Habib Pangeran Hamisya Nurindriani Haroh, Istik Hazmi, Nubli Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Indah Nurcahyani Iqbal Purnama, Muhammad Irene Putri Pricillia Butar-Butar Irene Putri Priscilla Butar-Butar Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Karina Karina Karina Karina Karina Komang A Wahyuningsih Komang A Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Linda Oktavianingsih Linda Oktavianingsih Masayu Nadhira Muhammad Fauzi Arif Muhammad Fauzi Arif Muhammadiyah Muhammadiyah Mukh Syaifudin Ngatmari Nita Noriko Nova Hariani Nur Ulmi Olivia Yolanda Lawono Panggulu Ahmad Ramadhani Utoro Pratiwi, Anggita Endar Putranto, Bima Arya Putri Inayah Aisyah Rahayu, Sekar Riris Lindiawati Puspitasari Rizka Shofiyya Ramadhani Rosliana, Iis Rudy Agung Nugroho Satriya Bayu Aji, Satriya Bayu Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Sobariah, Siti Sugianur, Sugianur Supandji, Supandji Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Umi Mutfidasari Umilasari, Reni Wafif Azizah Wahyuningsih, Komang A Widi Artini Widyastuti, Tias Wismo Reja Subroto Yuliani, Ferdina Yuliatin, Ervinda