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Determination of Total Phenolic Content and Antioxidant Activity of Epigallocatechin Gallate Rich Fraction from Tea Leaves (Camellia sinensis L.) Amelia, Puteri; Citraningsih, Kinanti; Dewi, Asri Kusuma; Anggia, Vivi; Anggraeni, Yuni
JSFK (Jurnal Sains Farmasi & Klinis) Vol 12 No 1 (2025): J Sains Farm Klin 12(1), April 2025
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.12.1.36-40.2025

Abstract

The tea plant, Camellia sinensis L., is a well-known natural product with significant health advantages. The main catechin found in green tea leaves is epigallocatechin gallate (EGCG), which has potent antioxidant properties. EGCG is a common active ingredient in products from the medical and cosmetics industries. This study examines the antioxidant properties and total phenolic content (TPC) of an EGCG-rich fraction (ERF) that is extracted from green tea leaves. TPC was quantified using the Folin-Ciocalteu method, with absorbance measured via UV-VIS spectrophotometry at 760.5 nm. Antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, with absorbance recorded at 516 nm. The findings indicated that the TPC of ERF was 437.18 mg GAE/g ± 5.54, while the IC50 value for antioxidant activity was 4.47 mg/L, This value was lower than that of the positive control, Vitamin C, which exhibited an IC₅₀ of 5.07 mg/L. These results suggest that ERF could be developed into a natural ingredient for cosmetics and dietary supplements.
Pembuatan Nutrisi dan Penyuluhan Penyakit Hipertensi pada Anggota PKK Delima Jakarta Timur Anggia, Vivi; Wiyati, Tuti; Wulandari, Nora
Jurnal SOLMA Vol. 8 No. 1 (2019)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/solma.v8i1.3059

Abstract

Menurut data SUDINKES DKI Jakarta tahun 2016 menunjukkan bahwa kotamadya Jakarta Timur memiliki jumlah populasi yang hipertensi lebih banyak dibandingkan dengan daerah lainnnya yaitu sebanyak 98422 orang. Konsumsi buah dan sayur setiap hari memberikan banyak manfaat bagi tubuh kita, bukan saja rasanya yang enak tapi buah dan sayur kaya akan antioksidan yang bermanfaat untuk mencegah terjadinya stres oksidatif dan bisa menjadi salah satu upaya dalam mencegah dan mengobati hipertensi. Berbagai buah dan sayuran banyak yang telah diteliti memberikan khasiat antihipertensi. Pada pengabdian masyarakat ini kami mengadakan Pelatihan pembuatan nutrisi dan penyuluhan penyakit hipertensi pada anggota PKK RT. 014 Perumnas Klender Jakarta Timur. Buah-buahan dan sayur-sayuran yang kami gunakan adalah belimbing, semangka, lemon, seledri dan mentimun.
Antibacterial Activity Against Acne-Causing Bacteria and Compound Profiling of Bacteria Associated with the Black Gum Sea Cucumber (Holothuria leucospilota) Arumawati, Novia Ashilia; Janatiningrum, Isra; Fitriyanti, Fitriyanti; Anggia, Vivi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 31, No 1 (2026): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.31.1.79-86

Abstract

Sea cucumbers (Holothuria leucospilota) have significant potential as sources of antibacterial compounds. These organisms live in association with bacteria that produce secondary metabolites exhibiting biological activities similar to those of their hosts, including antibacterial effects. Acne is an inflammatory condition that can be exacerbated by acne-causing bacteria such as Staphylococcus epidermidis and Propionibacterium acnes. Although antibiotics are commonly used for treatment, their effectiveness has increasingly been compromised by the emergence of antibiotic resistance. Bacteria associated with H. leucospilota therefore represent a promising alternative source of antibacterial agents due to their ability to produce bioactive secondary metabolites. This study aimed to characterize the macroscopic and microscopic diversity of these bacteria, evaluate their antibacterial activity against S. epidermidis and P. acnes, and analyze the chemical profiles of their ethyl acetate fractions. Bacterial isolation was conducted using Sea Water Complete Agar (SWCA), and antibacterial activity was assessed using the TLC bioautography method with iodonitrotetrazolium chloride (INT) as a growth indicator. A total of 12 bacterial isolates were obtained, comprising 10 isolates from the internal tissues and 2 isolates from the external surface of H. leucospilota. Antibacterial screening showed that 11 of the 12 isolates were active against S. epidermidis, while 8 isolates exhibited activity against P. acnes. Phytochemical screening indicated that phenolic and terpenoid compounds were the bioactive constituents responsible for the observed antibacterial activity.