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Journal : JURNAL BIOLOGI INDONESIA

Pengaruh Aplikasi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW Terhadap Infeksi Fusarium oxysporum Schlecht f. sp. cubense (FOC) dan Pertumbuhan Tanaman Pisang (Musa acuminata) var. Cavendish Laili, Nur; Antonius, Sarjiya; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3660.313 KB) | DOI: 10.14203/jbi.v11i1.2157

Abstract

Fusarium oxysporum Schlecht f. sp. cubense (Foc) is the causal pathogen of wilt disease of banana. Abilities of  biocontrol agents Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW to control Foc infection in banana were studied. Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or their combination in banana were tested under greenhouse conditions for 30 days. The aims of this study were to evaluate the potential of  Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents in banana. Treatments of biocontrol showed significant effect on the reduction of foc infection diseases of banana, compared to control. The lowest disease severity was found on the treatment of single isolate Streptomyces sp.L.3.1-DW with infection degree of 29,33%. Streptomyces sp. L.3.1-DW could suppress Foc population (6,25 x 105 CFU/ml) in rhizosphere area after 30 days innoculation. Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were also act as plant growth-promoting rhizobacteria (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.  This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW have potential as alternative solutions to control Fusarium wilt in banana var. Cavendish. Keywords: Bacillus sp. 140-B, banana, biocontrol, Foc, PGPR, Streptomyces sp. L.3.1-DW. 
Variasi Jumlah Kromosom Planlet Taraxacum officinale Weber ex FH. Wigg Hasil Regenerasi in vitro dari Eksplan Akar, Helai Daun dan Tangkai Daun Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.648 KB) | DOI: 10.14203/jbi.v10i2.2081

Abstract

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome
Co-Authors Abinawanto Abinawanto Achmad Dinoto Adinugroho, Wahyu Catur Agus Haryadi Anggita R Hafsari Anna Widyastuti Ariyanti Oetari Arrofaha, Nawwall Asep Sadili Asep Sadili Asep Sadili, Asep Atria, Mega Az Zahra, Najwa Wafiyah Bayu Adjie Brearley, Francis Q Citra Karina Windarti Decky Indrawan Junaedi Decky Indrawan Junaedi Dee Dee Al Farishy Dian Hendrayanti Dika Migi Priyono Dwiranti, Astari Edi Mirmanto Edi Mirmanto Edi Mirmanto, Edi Edi Mirtanto Endang Sukara Fadilah, Nurrahmi Fifi Gus Dwiyanti Firdha Meidi Irshanty Francis Q Brearly Iriani, Nur Annisa Irshanty, Firdha Meidi Irsyad Kamal ISKANDAR ZULKARNAEN SIREGAR Isnaenisa Rachma Khoiriyah, Istatik Kusumadewi Sri Yulita Kuswata Kartawinata Kuswata Kartawinata KUSWATA KARTAWINATA Lulut Dwi Sulistyaningsih Mansur Halik Marlina Ardiyani Maryanto, Andi Eko Mouleidi Dwi Putri MUHAMMAD MANSUR Muhammad Mansur Mutia Syadewi Najwa Wafiyah Az Zahra Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda NUNIK SRI ARIYANTI Nur Laili Pipit Marianingsih Pipit Marianingsih Pipit Marianingsih Priyono, Dika Migi Purba, Eliza Pricilia Puspitaningrum, Hardini Putrika, Afiatry Rachmat, Henti Hendalastuti Rahmadina, Amara Rahmandika, Ghifari Ririn Oktarina Saifudin Saifudin Sarah Tsabituddinillah Sarjiya Antonius Siska Apriliani Sulistiani Sulistiani, Sulistiani Syaputra, Dhika TRI JOKO SANTOSO Tri Joko Santoso Tri Joko Santoso Tri Muji Ermayanti Tsabitudinillah, Sarah Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Winarni, Nurul Laksmi Windri Handayani Windri Handayani Windri Handayani Wita Wardani Wita Wardani Yuki Ichinose Yuki Ichinose Yuniati, Ratna