Article Per Year (5 Year)
p-Index From 2021 - 2026
1.431
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian dan Pengembangan Pertanian Jurnal Tanaman Industri dan Penyegar Indonesian Journal of Disability Studies BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Biologi Tropis ANNALES BOGORIENSES AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Journal La Lifesci Makara Journal of Science Buitenzorg: Journal of Tropical Science Annales Bogorienses
TRI JOKO SANTOSO
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertania
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Veterinary

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : Makara Journal of Science

 1 
Screening and Integration Analysis of OsDREB1A BC4F2 and BC5F1 Generations of Transgenic Ciherang Rice (Oryza sativa L.) for High-Salinity Tolerance Priyono, Dika Migi; Santoso, Tri Joko; Salamah, Andi
Makara Journal of Science Vol. 17, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salinity is one of a number of abiotic stresses that threaten rice production in Indonesia. To support food security programs, BB-Biogen has developed rice lines derived from OsDREB1A transformed Ciherang up to BC4F2 and BC5F1 generations. To verify the salinity tolerance and the stability of transgene integration, the BC4F2 and BC5F1 generations of Ciherang-OsDREB1A transgenic were screened to select high-salinity tolerant lines. Second, molecular analysis using the primers hptII-F/hptII-R and 35S-496-F/OsDREB1A-R was conducted to identify the existence and the stability of the transgene integration in the BC4F2 and BC5F1 generations. Screening 543 BC4F2 and BC5F1 Ciherang-OsDREB1A transgenic lines in a nutrient solution with a final electrical conductivity (EC) of approximately 18 mS/cm for 26 days yielded 134 putative transgenic plants. Integration analysis using the hptII-F/hptII-R primers showed that 73 of the 134 putative transgenic plants had positive PCR products, indicating the presence of the transgene in those plants. All the 73 plants also produced PCR products when tested with the specific primer 35S-496- F/OsDREB1A-R, indicating that transgene integration was maintained during the development of BC4F2 and BC5F1.
Morphological and Molecular Analysis and Flowering Time of T2 Generation Transgenic Rice cv. Nipponbare Carrying CONSTANS (CO) Gene Irshanty, Firdha Meidi; Salamah, Andi; Santoso, Tri Joko
Makara Journal of Science Vol. 8, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Flowering is an important process that initiates plant productivity. Rice (Oryza sativa cv. Nipponbare) was genetically engineered by introducing a CONSTANS (CO) gene from Arabidopsis thaliana (AtCO) to improve rice productivity. The CO gene is a gene responsible for inducing early flowering. The aims of the experiment were to use morphological and molecular analysis to study the integration of the AtCO gene and its influence on agronomic traits in the T2 generation of Nipponbare transgenic AtCO rice. Morphological observations showed that agronomic traits tended to be better in the transgenic plants than in the wild type (controls), although the transgenic plants flowered later than the wild type. Polymerase Chain Reaction (PCR) analysis showed that 169 out of 227 transgenic rice plants showed hpt and CO gene integration.
Morphological and Molecular Analysis and Flowering Time of T2 Generation Transgenic Rice cv. Nipponbare Carrying CONSTANS (CO) Gene Irshanty, Firdha Meidi; Salamah, Andi; Santoso, Tri Joko
Makara Journal of Science Vol. 18, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Flowering is an important process that initiates plant productivity. Rice (Oryza sativa cv. Nipponbare) was genetically engineered by introducing a CONSTANS (CO) gene from Arabidopsis thaliana (AtCO) to improve rice productivity. The CO gene is a gene responsible for inducing early flowering. The aims of the experiment were to use morphological and molecular analysis to study the integration of the AtCO gene and its influence on agronomic traits in the T2 generation of Nipponbare transgenic AtCO rice. Morphological observations showed that agronomic traits tended to be better in the transgenic plants than in the wild type (controls), although the transgenic plants flowered later than the wild type. Polymerase Chain Reaction (PCR) analysis showed that 169 out of 227 transgenic rice plants showed hpt and CO gene integration.
Co-Authors A.A. Ketut Agung Cahyawan W Agus Purwito Agus Zainudin Akhdya, Alina Akhmad, Khabib Alia Ali Husni Andari Risliawati, Andari Andi Salamah Aniversari Apriana Anky Zannati Apriadi Situmorang Atmitri Sisharmini Bambang Sapta Purwoko Budi Martono Chaerani Chaerani Chaireni Martasari Dewi Sukma Dwi Astuti Dwi Widyajayantie DWINITA WIKAN UTAMI Eka Rosyida Enny Randriani Enung Sri Mulyaningsih Heri Syahrian, Heri Hilman Maulana Irshanty, Firdha Meidi KRISTIANTO NUGROHO, KRISTIANTO Kurniawan Rudi Trijatmiko, Kurniawan Rudi M. Khais Prayoga, M. Khais Maemunah Maemunah Mastur Mastur Mia Kosmiatin Nurul Hidayatun Pantouw, Carla F. Pantouw, Carla Frieda Priyono, Dika Migi PUJI LESTARI Rachmawati, Syamsidah Raden Mohamad Herdian Bhakti REFLINUR REFLINUR Samliok Ndobe Satya Nugroho Shabri Shabri, Shabri Sintho Wahyuning Ardie Solly Aryza Sri Indrayani, Sri Syafaruddin . Syamsidah Rahmawati, Syamsidah Trikoesoemaningtyas Triyani Dewi Vincentia Esti Windiastri, Vincentia Esti Vitria Puspitasari Rahadi Widyajayanti, Dwi Zakirah Raihani Ya’la