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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya UNEJ e-Proceeding Public Health of Indonesia Majalah Kesehatan FKUB
Teguh Wahju Sardjono
Parasitology Department, Medical Faculty, Brawijaya University
Agriculture, Biological Sciences & Forestry Education Environmental Science Medicine & Pharmacology Public Health

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Journal : Jurnal Kedokteran Brawijaya

 1 
Kejadian Toxoplasmosis pada Kasus-kasus Abortus Spontan di RS Dr. Saiful Anwar dideteksi dengan Pemeriksaan Serologik, Histopatologik dan PCR Sardjono, Teguh Wahju; Soewarto, Soetomo; Muhammad, Lubnah
Jurnal Kedokteran Brawijaya Vol 18, No 2 (2002)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2048.369 KB)

Abstract

Toxoplasmosis dikenal sebagai salah satu penyebab penting dari abortus spontan pada manusia, tetapi seberapa besar sebenarnya peran dari penyakit ini sebagai penyebab abortus, belum diketahui secara pasti. Penelitian ini dilakukan untuk mengetahui angka kejadian Toxoplasmosis pada kasus-kasus abortus spontan di RSUD dr. Saiful Anwar (RSSA) Malang, dengan menggunakan metode pemeriksaan serologic, histopatologik dan PCR. Dalam kurun waktu 4 bulan (Maret-Juni 2000) dari 2395 kunjungan pasien di bagian Obstetri-Ginekologi RSSA didapatkan 451 kasus abortus (18,83%). Diperoleh 43 kasus abortus spontan dan 23 kaus kehamilan normal yang memenuhi syarat dan bersedia mengikuti penelitian ini. Pada kelompok kehamilan normal, rerata kadar IgM maupun IgG ternyata lebih tinggi dibanding kelompok subyek, tetapi perbedaan tersebut tidak bermakna (p>0,05). Rasio prevalensi kasus dengan IgM (-) dan IgM (+) pada kelompok pembanding (1:22) dibandingkan pada kelompok studi (3:40) tidak berbeda secara bermakna (p=0,67; OR=1,65). Rasio prevalensi kasus dengan IgG (+) dan IgG (-) pada kelompok pembanding (14:9) lebih besar dari kelompok subyek (22:21), tetapi perbedaan ini juga tidak bermakna (p=0,45; OR=0,67). Pada penelitian ini tidak ditemukan adanya Toxoplasma di jaringan kerokan sisa kehamilan, baik melalui pemeriksaan histopatologik maupun PCR. Hasil penelitian ini tidak dapat menyimpulkan bahwa Toxoplasmosis merupakan penyebab utama dari abortus spontan. Wanita hamil dengan IgG Toxoplasma positif justru mempunyai resiko lebih rendah untuk mengalami abortus dibandingkan yang IgGnya negatif.
KEGAGALAN KEHAMILAN AKIBAT INFEKSI Toxoplasma gondiiGALUR RH DIPICU OLEH OVERPRODUKSI INTERFERON GAMMA (IFNγ γγ γ) Sardjono, Teguh Wahju
Jurnal Kedokteran Brawijaya Vol 21, No 3 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.428 KB) | DOI: 10.21776/ub.jkb.2005.021.03.5

Abstract

It has been already known that transplacental infiltration of parasites occurred during Toxoplasma infection in pregnancy; but pregnancy failures may due to some other previous mechanisms. Eighty BALB/c female mice were monogamously mated than devided into three pairs of study groupsand a pair of control group. On Day 9thpost mating, three pairs of study groups were intra-peritoneally infected by 10, 50 and 100 tachyzoites of RH strain Toxoplasma gondii, and the control groups were injected by PBS. Effect of treatment on the plasma level of IFNγ(ELISA) and number of litters were evaluated on the day 12thand day 15th-16th, represented second and third period of pregnancy.ANOVA and Path Analysis showed that duration of infection and the increase  of plasma IFNγ level decreased number of litters with coefficients of path of (-0,269; p=0,005) and (-0,377; p= 0,002) respectively. Decreasing number of litters was due toIFNγoverproduction, as response against RH strain of Toxoplasma gondii infection. This study showed that pregnancy failure in Toxoplasma infection is more due to indirect immuno-biochemical aspects rather than direct parasitological aspect. More studies to explain the basic molecular and immunological mechanisms of pregnancy failure in toxoplasmosis are still needed. Key words:Toxoplasmosis, IFNγ-overproduction, pregnancy failure
PERAN LABORATORIUM DALAM DIAGNOSIS DAN PENATALAKSANAAN KASUS KASUS PENYAKIT TROPIK DAN INFEKSI Sardjono, Teguh Wahju; Ismanoe, Gatoet; Widjayanto, Edi
Jurnal Kedokteran Brawijaya Vol 20, No 1 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.874 KB) | DOI: 10.21776/ub.jkb.2004.020.01.4

Abstract

Infectious disease is still a health problem in theworld, especially in tropical and developing countries, including Indonesia. The diagnosis of tropical disease cases mostly are still based on the main of clinical symptoms, that is fever. Among 900 fever patients, 21% of the disease were diagnosed by clinical examinations, 35% by laboratory tests and 40% were diagnosed as fever of unknown origin (FUO). One forth of the FUO patients were definitively diagnosed after specific laboratory tests, but the use of the specific tests in daily practice were relatively rare. The tests ordered by physicians to the Department of Central Clinical Laboratory Dr. Saiful Anwar Hospital Malang, are mostly hematology and blood chemical tests (59.54% and 21.52%). Specific tests for microbiology and parasitology were only 3.15% and 0.46% respectively. Fever patients who not definitively diagnosed, often received unnecessary treatments or medications (over-treatment), those are actually dangerous to them. To improve the quality of clinical management, the traditional laboratory service should be changed tobe the interactive laboratory service. It needs a  role, understanding and good collaboration between clinical practitioners who know the basic sciences and the pre/para-clinical scientists who interest to the clinical phenomenon. The aim of the change is to get the more scientific, ethical and professional medical services. Key words:Laboratory medicine, diagnosis,management, infectious disease
High Level of Plasma Tumour Necrosis Factor-α (TNF- α) in Pregnant Mice Infected with Plasmodium berghei is Strongly Related to Low Level of Hemoglobin but not Related to Fetal Low Weight Yuliyanik, Yuliyanik; Fitri, Loeki E; Sardjono, Teguh Wahju
Jurnal Kedokteran Brawijaya Vol 28, No 4 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2015.028.04.3

Abstract

Malaria infection in pregnancy may increase the morbidity and mortality for both mother and fetus. In pregnant women, it can lead to severe anemia, cerebral malaria, pulmonary edema, renal failure and even death, while in the fetus it can cause abortion, premature birth, low birth weight, and fetal death. Elevated levels of tumor necrosis factor-α (TNF-α ) are associated with low birth weight and anemia in pregnant women. This study was conducted to measure the levels of TNF-α in plasma and hemoglobin levels as well as fetal weight to determine the relationship among them in P. berghei infected pregnant mice and normal pregnant mice. Seventeen BALB/c mice used in this study were divided into two groups, those were the study group (9 pregnant mice infected with P. berghei) and control group (8 pregnant mice not infected with P. berghei). Levels of TNF-α were measured using Enzyme Linked Immunosorbent assay (R&D Systems, catalog A00B MT). Hemoglobin levels were determined using flowcytometri, whereas fetal weights were measured with Mettler analytical balance AE 50. T-test statistical analysis showed that the levels of plasma TNF-α in study group were higher than control group (p=0,000). Hemoglobin levels in the study group  were lower than control group (p=0,025). Fetal weights were also lower in fetuses of infected mice than fetuses of uninfected mice (p=0,002). Pearson correlation test showed that increasing plasma levels of TNF-α in infected P. berghei pregnant mice were related with the decreasing levels of Hb, (p=0,020; r=-0,748).  However plasma levels of TNF-α were not associated with the incidence of fetal low weight (p=0,380, and p=0,365). It can be concluded that the increasing levels of TNF-α is associated with decreasing levels of hemoglobin (Hb), but not associated with fetal low weight.Keywords:  Birth weight, hemoglobin, Plasmodium berghei, Tumour Necrosis Factor-α (TNF- α)
Co-Authors Adilah Ulfiati, Adilah Ardhian Wardana, Ardhian Edi Widjayanto Effendy, Meyland Citra Oktri Sienty Eviana Norahmawati Fitriana Nugraheni, Fitriana Fransiska Imavike Fevriasanty Gatoet Ismanoe Gusti Agung I.P, Gusti Agung Harry Kurniawan Gondo I Gusti Ngurah Juniartha Kartika Senjarini Kartikawatiningsih, Dewi Loeki E Fitri, Loeki E Loeki Enggar Fitri Moh Mirza Nuryady, Moh Mirza Muhammad, Lubnah Nugraha, Rivo Yudhinata Brian Nur Fahma Pradiptasari, Nur Fahma Putri, Anisa Tiara Soetomo Soewarto Sugeng Setyo Utomo, Sugeng Setyo sujarot dwi sasmito, sujarot dwi Yuliyanik yuliyanik Yunita Armiyanti Zakiyah Zulaifa, Zakiyah