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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya UNEJ e-Proceeding Public Health of Indonesia Majalah Kesehatan FKUB
Teguh Wahju Sardjono
Parasitology Department, Medical Faculty, Brawijaya University
Agriculture, Biological Sciences & Forestry Education Environmental Science Medicine & Pharmacology Public Health

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The Use of Natural Phenomenon in Obtaining Pregnant Rats and Mice as Experimental Animals with The Same Gestational Ages Sardjono, Teguh Wahju; Gondo, Harry Kurniawan; Nugraha, Rivo Yudhinata Brian; Putri, Anisa Tiara; Effendy, Meyland Citra Oktri Sienty
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Hormonal oestrus synchronization is often used to obtain the same gestational ages animal models, but it may not physiologic nor practical. This research was conducted to find out a simple method in obtaining physiologic pregnant experimental animals. We used 120 female rats and mice each, divided into 16 groups with 15 members each. They were classified according to the state of parities, pair or group mating, and synchronization of oestrous. The estrous synchronization is done by utilizing the natural phenomenon (Lee-Boot, Pheromone, and Whitten effect). All nulliparous and multiparous models then were simultaneously mated for one night in pairs (1:1) or in groups (1:5) subsequently. Pregnancy rates were noted based on the number of models delivering pups on day 19-23 post-mating. The highest pregnancy rate was noted in group of multiparous, synchronized and monogamous rats (86.7%), followed by nulliparous, synchronized and monogamous rats (73.3%) and multiparous, synchronized and monogamous mice (60%) (p<0.05). Synchronization of the estrous and monogamous mating of rats or mice had significant roles in pregnancy rates (p<0.05). To get a high number of pregnant rats or mice models, we suggest to use the multiparous, estrous synchronized and monogamous simultaneous mating within one night.
Endoglin Expression and the Level of Tgf-Î’ Are Increased In the Placental Tissue and Correlated with Low Fetal Weight in Malaria Infected Mice sasmito, sujarot dwi; Ulfiati, Adilah; Wardana, Ardhian; Nugraheni, Fitriana; Pradiptasari, Nur Fahma; Zulaifa, Zakiyah; Norahmawati, Eviana; Sardjono, Teguh Wahju; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 5, No 1 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.5.1.%x

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Malaria infection during pregnancy can cause accumulation of infected-red blood cells in placental intervillous space and induces placental tissue inflammation and hypoxia. This condition triggers endoglin expression, and release of soluble endoglin which can interfere TGF-β binding to its receptor. The aim of this study was to investigate the correlation between placental endoglin expression and TGF-β level with low fetal weight (LFW) in malaria-infected mice. Nine pregnant mice infected with Plasmodium berghei on the day 9th post mating (malaria infected group) and eight normal pregnant mice (non-infected group) were used in this study. The mice were sacrificed on the day 18th post mating, and all fetal body weights were measured by analytical scale. Enzyme Link Immunosorbent Assay (ELISA) was done to determine the level of placental TGF-β while immunohistochemical staining was performed to examine endoglin expression in placental tissue. The mean of fetal body weights of malaria infected group was significantly lower than non-infected group (p=0,002), while the expression of placental endoglin in malaria infected group was significantly higher than non-infected group (p=0.003). The level of placental TGF-β in malaria infected group was also higher than non-infected group but the difference was not significant (p=0.064). Pearson correlation test showed that there were significant negative correlations between fetal body weights with the level of placental TGF-β (p=0.017, r=-0.568) and the expression of placental endoglin (p=0.002, r=-0.694). Malaria infection in pregnant mice will increase both TGF- β and endoglin in placenta tissue and correlate with low fetal weight.   
Co-Authors Adilah Ulfiati, Adilah Ardhian Wardana, Ardhian Edi Widjayanto Effendy, Meyland Citra Oktri Sienty Eviana Norahmawati Fitriana Nugraheni, Fitriana Fransiska Imavike Fevriasanty Gatoet Ismanoe Gusti Agung I.P, Gusti Agung Harry Kurniawan Gondo I Gusti Ngurah Juniartha Kartika Senjarini Kartikawatiningsih, Dewi Loeki E Fitri, Loeki E Loeki Enggar Fitri Moh Mirza Nuryady, Moh Mirza Muhammad, Lubnah Nugraha, Rivo Yudhinata Brian Nur Fahma Pradiptasari, Nur Fahma Putri, Anisa Tiara Soetomo Soewarto Sugeng Setyo Utomo, Sugeng Setyo sujarot dwi sasmito, sujarot dwi Yuliyanik yuliyanik Yunita Armiyanti Zakiyah Zulaifa, Zakiyah