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THE EFFECT OF MELON SEEDS FLOUR (Cucumis Melo L.) TOWARD TOTAL CHOLESTEROL LEVEL IN WHITE MALE WISTAR STRAIN RATS (Rattus Norvegicus) Maruni Wiwin Diarti; Erlin Yustin Tatontos; Yunan Jiwintarum; Anggun Sari Mianti
Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Cholesterol is an important element in the body, but in high quantities, it can cause atherosclerosis that will eventually possess an impact on coronary heart disease. One of the foods that can reduce cholesterol is a food contains flavonoids which is found in melon seeds (Cucumis melo l.). It contains high fiber that can reduce either of excessive insulin or blood fat levels. The objective study was to determine the effect of Melon seeds flour Toward Total Cholesterol Level in White Male Wistar Strain Rats. This research was carried out with pre-expriment research design, used One group pretest-postest with 5 male wistar strain rats. Data collected in the form of data examination results of total cholesterol level before and after introducing Melon seeds flour. The mean result of total cholesterol test in white male rats before giving Melon seeds flour was 261 mg / dL, whereas the result of total cholesterol test after giving Melon seeds flour was 190.2 mg/dL. Cholesterol levels decreased by 70.8 mg/dl. In short, presenting Melon seeds flour (Cucumis melo l.) for 14 days can decrease total cholesterol level in white male wistar rats. Keywords: Melon Seeds, wistar strain, total cholesterol.

THE IMMUNE RESPONSE IN WORM INFECTION AT PUSKESMAS TANJUNG KARANG IN MATARAM Gunarti Gunarti; Erlin Yustin Tatontos; Urip Urip
Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstrac: A worm infection can decrease sufferers’ health condition, nutrition, intelligence and their productivity thus, it can cause numerous of disadvantages economically. The immune response to worm infection begins with Th2 activation with a significant increase of IL-4, IL-5, IL-9, IL-10 and IL-13. The cytokines IL-5 that formed stimulates the growth and activation of eosinophils. The purpose of this research was to know the immune response by increasing the number of eosinophils to worm infection rate at Puskesmas Tanjung Karang. The research design was expost facto with cross sectional or transversal. The sample was communities’ faces and blood at Puskesmas Tanjung Karang as many as 50 people. The technique sampling was random from family members who have worm risk factors (poor sanitation conditions, living near animal cage, ground floor houses, pets such as dogs and cats). The correlation analysis of worm infection with eosinophils used Mann Whitney Test. The results showed that the prevalence of worm infection belong to mild category for Trichurin trichura was 10%, and the overall worm infection was 12% whereas the average number of eosinophils in the normal category was between 50-300 cells / mm². The results of statistical analysis were not significant (Asymp. Sig. 0.059) which means that there was no effect of the immune response with the number of eosinophils on the level of worminfection at Puskesmas Tanjung Karang.Keywords: Immune response, eosinophil, worm infection.

THE ALTERNATIVE DILUTE SOLUTION OF NaCl 0.9% AT THE GIEMSA STAINING ON THE INVESTIGATION THE MORPHOLOGY OF SPERMATOZOA Maruni Wiwin Diarti; Erlin Yustin Tatontos; Aden Turmuji
Jurnal Kesehatan Prima Vol 10, No 2 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract : The quality of staining, the morphology of spermatozoon applays The Giemsa dye, is strongly influenced by the type of Giemsa thinner. The terms of diluent that can be used is having a buffer property. The solution of NaCl 0.9 %, besides having buffer properties also has a cheaper price. The research is a descriptive study that aims to determine NaCl 0.9% as the alternative of diluting solution at Giemsa staining on morphological examination of spermatozoon. This study was conducted to compare the quality of spermatozoon morphology staining by using Giemsa which was diluted with 0.9% NaCl solution and the solution of Phosphate Buffer. The result of the study indicated The Giemsa staining with NaCl 0.9% solution, is obtained the outstanding spermatozoon morphology staining absorption was 71.625% and the poor spermatozoon morphology staining absorption was 28.375%. The results of study which was applied the Phosphate Buffer solution, was obtained the outstanding spermatozoon morphology staining absorption was 81.563% and the poor spermatozoon morphology staining absorption was 18.438%. The NaCL 0.9% can be used as an alternative dilute solution at Giemsa staining on morphological examination of spermatozoon.

Pengaruh Jenis Sampel Urine Terhadap Pemeriksaan HCG (Human Chorionic Gonadotropin) Untuk Deteksi Kehamilan Dini Metode Immunokromatografi Wardani, Yuliana; Erlin Yustin Tatontos; Fihiruddin; Yunan Jiwintarum
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.129

Latar Belakang : Kehamilan merupakan suatu proses yang akan dialami oleh hampir semua wanita. disaat sel telur bertemu dengan sperma dan terjadi pembuahan dapat menyebabkan kehamilan. Human Chorionic Gonadotropin (HCG) adalah hormone yang diproduksi oleh trophoblast pada awal kehamilan yang dikeluarkan melalui urine. Adanya hormone HCG dalam urine dapat digunakan untuk deteksi kehamilan dini. Urine pagi baik untuk pemeriksaan kehamilan atau deteksi HCG karena, urine pagi merupakan urine satu malam yang mencerminkan periode tanpa asupan cairan yang lama, sehingga urine pagi lebih pekat daripada urin sewaktu yang konsentrasinya lebih encer sehingga sulit untuk mendeteksi hormone HCG. Tujuan Penelitian : Mengetahui pengaruh jenis sampel urine terhadap pemeriksaan Human Chorionic Gonadotropin (HCG) dalam deteksi kehamilan dini metode immunokromatografi. Metode Penelitian : Penelitian ini merupakan penelitian dengan metode observasional analitik dengan pendekatan cross sectional. Sampel dalam penelitian ini adalah wanita yang terlambat menstruasi 1-3 minggu (hamil) pada wilayah kecamatan selong dengan jumlah sampel sebanyak 24 sampel. Analisa data menggunakan Analisa deskriptif. Pada penelitian ini menggunakan metode Immunokromatografi (Testpack). Hasil Penelitian : Didapatkan hasil positif (+) pada 12 sampel urine pagi hari dan didapatkan hasil positif (+) pada 12 sampel urine sewaktu wanita yang terlambat menstruasi 1-3 minggu. Kesimpulan : Tidak terdapat pengaruh jenis sampel urine dalam deteksi HCG menggunakan sampel urine pagi hari dan urine sewaktu pada wanita yang terlambat menstruasi 1-3 minggu.

Hubungan antara Lama Terinfeksi Virus Penderita Hepatitis B dengan Indeks Eritrosit (MCV,MCH,MCHC) Septiani, Erna; Maruni Wiwin Diarti; Erlin Yustin Tatontos; Fihiruddin
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 2 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i2.166

Background: Hepatitis B is an infectious disease that attacks the liver caused by the Hepatitis B virus. Hepatitis B virus infection is very infectious and is the main cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Hepatitis B can cause liver cirrhosis, where there is a change in the structure of the liver parenchyma resulting in a decrease in erythrocyte index levels. Objective: To determine the relationship between the duration of viral infection in hepatitis sufferers and the erythrocyte index (MCV, MCH, MCHC) Method: This research is an analytical observational study with a cross sectional approach and accidental sampling technique Results: Based on the results of the Kruskall Wallis test, the relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV) resulted in MCV 75.50, MCH 17.97, MCHC 43.03 with a p value = 0.000 < 0.05. so it can be concluded that there is a relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV) Conclusion: From the results of the analysis carried out, there is a relationship between the duration of infection with the hepatitis B virus and the erythrocyte index (MCV, MCH, MCHV)

Gambaran Hasil Aglutinasi Pemeriksaan Widal Slide Metode Semi Kuantitatif pada Pasien Demam Tifoid di Puskesmas Kediri Khairunnisa; Yunan Jiwintarum; Ida Bagus Rai Wiadnya; Erlin Yustin Tatontos
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.184

Latar Belakang : Pemeriksaan Widal slide telah menjadi salah satu metode diagnostik yang umum digunakan untuk mendeteksi infeksi demam tifoid. Pada pemeriksaan Widal slide metode kualitatif, volume sampel yang digunakan tidak terukur sehingga penentuan hasil titer ditentukan dengan melihat banyak atau sedikitnya aglutinasi yang terjadi. Hal ini dapat menyebabkan hasil positif palsu terutama karena variasi interpretasi dan faktor-faktor lain yang dapat mempengaruhi hasil. Dalam penentuan hasil titer Widal slide perlu melakukan pengukuran volume sampel dan melakukan validasi hasil dengan pengenceran serum atau plasma menggunakan metode semi kuantitatif, sehingga pembacaan hasil titer dapat dilakukan dengan melihat aglutinasi terakhir yang terbentuk. Tujuan Penelitian : Mengetahui gambaran hasil aglutinasi pemeriksaan Widal slide metode semi kuantitatif pada pasien demam tifoid di Puskesmas Kediri. Metode Penelitian : Penelitian ini merupakan penelitian deskriptif analitik dengan populasi sampel penelitian adalah pasien demam tifoid di Puskesmas Kediri. Sebanyak 20 sampel penelitian diambil dengan teknik accidental sampling. Pengumpulan data diperoleh dengan pemeriksaan Widal slide pada pasien demam tifoid dan didapatkan gambaran hasil aglutinasi pada pemeriksaan Widal slide metode kualitatif dan metode semi kuantitatif. Hasil Penelitian : Didapatkan gambaran hasil aglutinasi pemeriksaan Widal slide metode semi kuantitatif dengan titer 1/80 yang menunjukkan aglutinasi terakhir pada sampel 20 µl, titer 1/160 menunjukkan aglutinasi terakhir pada sampel 10 µl, titer 1/320 menunjukkan aglutinasi terakhir pada sampel 5 µl, dan titer 1/640 menunjukkan aglutinasi terakhir pada sampel 2,5 µl. Kesimpulan : Gambaran hasil aglutinasi Widal slide metode semi kuantitatif dapat memberikan hasil titer lebih tepat daripada menggunakan metode kualitatif.

Uji Efektivitas Kombinasi Filtrat Daun Pepaya (Carica papaya) dan Daun Kemangi (Ocimum sanctum) Terhadap Daya Bunuh Kutu Kepala (Pediculus humanus capitis) Putu Dita Septiani; Urip; Erlin Yustin Tatontos; Ershandi Resnhaleksmana
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.78

Latar belakang: Kutu kepala (Pediculus humanus capitis) merupakan suatu ektoparasit obligat penghisap darah yang terdapat pada rambut atau kepala manusia. Penggunaan bahan kimia memiliki dampak buruk bagi kesehatan manusia apabila digunakan secara tidak tepat. Oleh karena itu dibuat bahan alami pembasmi kutu kepala yaitu kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) yang mudah ditemukan di masyarakat. Tujuan: Untuk mengetahui efektivitas kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) terhadap daya bunuh kutu kepala (Pediculus humanus capitis). Metode: Penelitian ini bersifat quasi eksperiment dengan desain penelitian post test only control group desain. Populasi dan sampel adalah kutu kepala (Pediculus humanus capitis). Jumlah unit percobaan 27 (3 perlakuan dengan 9 kali replikasi) masing-masing petridish menggunakan 5 ekor kutu rambut sehingga jumlah sampel yang dibutuhkan adalah 135 ekor. Perlakuan menggunakan kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) dengan perbandingan konsentrasi 30%:70%, 50%:50%, dan 70%:30% selama 60 menit serta kontrol negatif aquadest. Data yang dikumpulkan kemudian di analisis menggunakan uji Kruskal-wallis. Hasil penelitian: Didapatkan kematian kutu kepala kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) perbandingan konsentrasi 30%:70% dengan persentase kematian sebesar 48%, kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) perbandingan konsentrasi 50%:50% dengan persentase kematian sebesar 62%, dan kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) perbandingan konsentrasi 70%:30% dengan persentase kematian sebesar 80%. Kesimpulan: Kombinasi filtrat daun pepaya (Carica papaya) dan daun kemangi (Ocimum sanctum) efektif terhadap daya bunuh kutu kepala (Pediculus humanus capitis) dengan nilai signifikan p = 0,000 < α =0,05. Kata kunci: Kematian, Filtrat, Alami, Daun Pepaya, Daun Kemangi, Kutu Kepala.

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