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All Journal Jurnal Hortikultura Indonesia (JHI) Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Planta Tropika Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Nintya Setiari
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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The Effect of Thidiazuron Treatment on the Growth of Vanda limbata Blume in vivo Ditasya Putri, Novika; Setiari, Nintya; Nurchayati, Yulita; Agung Suedy, Sri Widodo
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at three concentrations (factor levels) of 0, 25, and 50 ppm. Data were analyzed using analysis of variance and Duncan’s Multiple Range Test at 5% significance level. The study ran for three months and the following response variables were measure: the number of new leaves, increase in length of old leaves, length of new leaves, increase in width of old leaves, width of new leaves, number of roots, and stomatal density. The results showed that TDZ significantly increased leaf length and the number of new leaves of V. limbata, but decreased the density of stomata. Thidiazuron at 50 ppm was the best treatment for increasing the growth of V. limbata.
Thidiazuron-Induced Somatic Embryogenesis in Cymbidium bicolor Orchid In Vitro Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.15306

Abstract

Cymbidium bicolor is a highly hunted and traded orchid, leading to a decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) is a growth regulator used to induce somatic embryogenesis. This study aimed to determine the optimal TDZ concentration for somatic embryo formation. Stem explants of C. bicolor were cultured on Murashige Skoog (MS) medium with TDZ concentrations of 0, 1, 2, and 3 ppm. Observations were conducted weekly for two months using a stereo microscope and OptiLab. Variables observed included the percentage of green explants, somatic embryo formation time, the number of explants forming somatic embryos, and the number and morphology of somatic embryos. The study was arranged in a Completely Randomized Design (CRD) with 14 replications. Results showed that TDZ addition influenced somatic embryo formation and maintained the green color of explants. Media with TDZ promoted faster growth and larger embryo size compared to media without TDZ. The optimal concentration was 1 ppm TDZ, which produced the highest number of embryos (172) and the fastest formation time compared to other concentrations (TDZ 0: 27, TDZ 2 ppm: 60, TDZ 3 ppm: 39).
Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP) Karend, Azzah Hanun Abas; Setiari, Nintya; Nurchayati, Yulita; Rashid, Fatimah Azzahra Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p88-98

Abstract

Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.
The Effect of Benzyl Amino Purine (BAP) On Potato (Solanum tuberosum L.) Axillary Buds Micropropagation Putri Anugrah Maulidya; Nurchayati, Yulita; Setiari, Nintya
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 01 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss01/480

Abstract

Potato (Solanum tuberosum L.) production in Indonesia increased by 4.21% in 2022 compared to the previous year, but this production is still low by international standards. This is caused by low quality and quantity of potato seeds which is sourced from tubers. It is necessary to modify the in vitro cultivation of potato seed sources by utilising seeds that are often wasted with the addition of cytokinins, such as Benzyl Amino Purine (BAP). The aim of this study was to determine the effect of BAP on potato axillary shoot explants sourced from seeds. The study was conducted using a Completely Randomized Design with one factor, namely BAP concentrations of 0, 1, 2 and 3 ppm with 3 replications. The results showed that there is an influence of BAP on the parameters of shoot growth percentage, leaf and root emergence time, plant height, number of shoots, roots, and leaves of potato axillary buds planlet. The 2 ppm BAP treatment tends to give the best response in increasing the number of shoots with an average of 4.33 shoots, making it effective for producing potato seedlings from axillary buds in vitro.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Pertumbuhan Anggrek Cymbidium ensifolium (L.) Sw. dengan Pemberian Pupuk Hayati Mikoriza Misrofah, Sofatun; Misrofah, Sofatu; Setiari, Nintya; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 1 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.1.35-42

Abstract

ABSTRAKAnggrek Cymbidium ensifolium adalah anggrek terestrial yang terdaftar di CITES 2020 sebagai spesies hampir terancam punah atau rentan sehingga perlu upaya pelestarian secara ex situ. Salah satu faktor pendukung budidaya anggrek adalah pemberian pupuk. Pupuk hayati mikoriza bersifat ramah lingkungan dan dapat mengurangi dampak negatif pupuk anorganik. Penelitian ini bertujuan untuk mengkaji pengaruh pupuk hayati mikoriza berbagai dosis terhadap pertumbuhan anggrek C. ensifolium. Penelitian ini disusun menggunakan Rancangan Acak Lengkap satu faktor, yaitu pupuk hayati mikoriza (0; 5; 10; 15 g tanaman-1) dengan 6 ulangan. Data dianalisis pada Analysis of Variance dengan taraf kepercayaan 95%. Percobaan dilakukan bulan Oktober 2021 – Februari 2022 di Tembalang, Semarang. Parameter yang diamati berupa waktu muncul tunas, jumlah tunas, waktu muncul daun, jumlah daun, dan persentase infeksi mikoriza. Hasil penelitian menunjukkan bahwa perlakuan pupuk hayati mikoriza berpengaruh signifikan terhadap waktu muncul tunas dan daun, jumlah daun, dan persentase infeksi mikoriza. Pupuk hayati mikoriza sebesar 5 g tanaman-1 merupakan perlakuan optimal untuk meningkatkan waktu muncul tunas dan daun, serta konsentrasi 15 g tanaman-1 optimal meningkatkan jumlah daun dan persentase infeksi mikoriza. Pengaruh pupuk hayati mikoriza ini dapat dikembangkan untuk pelestarian anggrek jenis lain. Kata kunci: anggrek spesies, Cymbidium, biofertilizer, mikoriza
The Physiological Response of Germination and Growth in Solanaceae Plants (Capsicum frutescens, Solanum melongena, Solanum lycopersicum) at Different Salinity Levels Della, Nelly Vikiladyla; Hastuti, Endah Dwi; Setiari, Nintya
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1352-1360

Abstract

High salinity causes osmotic stress and ion imbalance that can reduce plant productivity. Solanaceae can be developed for cultivation in saline land, but its growth is influenced by the type of species. This study aims to examine the tolerance level of three Solanaceae plants to salinity stress through observation of physiological responses of germination and growth. This study used a 3 x 4 factorial Completely Randomized Design (CRD). The first factor is salinity: 0 ppm, 2,500 ppm, 5,000 ppm and 7,500 ppm. The second factor is the Solanaceae species, namely Capsicum frutescens, Solanum melongena, and Solanum lycopersicum. Germination parameters include germination power, wet weight and dry weight. The growth parameters observed include plant height, root length, stem diameter, leaf area, number of leaves, wet weight of leaves, roots and stems and dry weight of leaves, roots and stems. The results of the study showed that C. frutescens is a plant that is more tolerant to salinity up to a concentration of 5,000 ppm when compared to S. melongena and S. lycopersicum whose tolerance is up to 2,500 ppm. Keywords: Germination, Salinity, Solanaceae, Vegetative Growth.
Pengaruh Media MS dan VW Terhadap Pertumbuhan Planlet Anggrek Bulan (Phalaenopsis amabilis L. Blume) Setelah Transplanting Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.971 KB)

Abstract

Anggrek merupakan jenis tanaman hias yang memiliki bunga khas dengan mahkota indah dan warna menarik. Anggrek Bulan (Phalaenopsis amabilis) merupakan bagian dari bunga nasional dan dijuluki “Puspa Pesona”. Ketersediaan Anggrek Bulan di alam mulai berkurang, sehingga diperlukan upaya konservasi. Pembiakan anggrek alam dapat dilakukan dengan teknologi tissue culture atau kultur jaringan biji. Salah satu metode penting dalam kultur jaringan yaitu proses transplanting. Tujuan transplanting agar planlet anggrek tersebut tetap terjaga pertumbuhannya. Penggunaan media tumbuh yang tepatmerupakan salah satu faktor yang menentukan keberhasilan transplanting. Tujuan dari penelitian ini untuk mengetahuipertumbuhan Anggrek Bulan pada dua media berbeda, yaitu MS (Murashige and Skoog) dan VW (Vacin and Went). Metode yang digunakan adalah transplanting planlet Anggrek Bulan umur 1 tahun hasil kultur jaringan dari biji ke media perlakuan MS dan VW tanpa ZPT. Selanjutnya planlet tersebut dipelihara di ruang inkubasi dengan cahaya 1000 lux dan suhu 25℃.Parameter yang diamati yaitu pertambahan jumlah tunas, jumlah daun, panjang daun, lebar daun, dan panjang akar. Pengamatan dilakukan setiap seminggu sekali. Hasil pengamatan menunjukkan bahwa media MS meningkatkan jumlah tunas dan panjang akar, serta mempercepat munculnya tunas baru yaitu satu minggu setelah transplanting. Simpulan dari penelitian ini adalah pertumbuhan planlet cenderung lebih baik di media MS dibanding media VW.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
THE GROWTH OF Dendrobium spectabile (Blume) Miq. ORCHID PLANTLET IN VACIN AND WENT (VW) MEDIUM WITH DIFFERENT CONCENTRATIONS OF COCONUT WATER Zakiyah, Aisyah; Setiari, Nintya; Nurchayati, Yulita
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.222

Abstract

Dendrobium spectabile is an epiphytic orchid that grows slowly and experiences natural exploitation, so it needs to be conserved ex-situ through in vitro culture. One of the essential steps for in vitro culture is subculture to supply the nutrients needed by plantlets. Increasing the growth of these plantlets can be done by adding coconut water. This study aimed to determine the effect of adding coconut water and the optimal concentration of coconut water on the growth of D. spectabile plantlets in VW medium. The method used is growing plantlets in VW medium with different concentrations of coconut water. The research design was completely randomized with a single factor, the concentration of coconut water (0%, 5%, 10%, 15%, 20%). Data were analyzed by ANOVA at a 5% significance level. Plantlet growth was observed for two months. Parameters observed were new shoots, leaves, and roots emergence time; number of new shoots, leaves, and roots; and new leaves length. The addition of coconut water affected the growth of D. spectabile orchid plantlets. Five to ten percent of coconut water is the optimal concentration for shoots and leaves growth of D. spectabile orchid plantlets. Therefore, coconut water can increase the growth of in vitro plantlets.
Co-Authors Abdul Khafid, Abdul Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Della, Nelly Vikiladyla Desi Arisanti Diah Nurmaningrum Ditasya Putri, Novika Einstivina Nuryandani Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Hartati, Puji Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas Luaeliyah, Masrukhatul Misrofah, Sofatu Misrofah, Sofatun Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Muhammad Syaiful Anam Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Septiyana, Eva Sri Darmanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Yulita Nurchayati Zahrotunnisa, Alfi Zakiyah, Aisyah