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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Planta Tropika Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Nintya Setiari
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health

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Rendemen Minyak Atsiri Rimpang Jahe Merah (Zingiber officinale var. rubrum) dengan Metode Pengeringan yang Dikombinasi dengan Kain Penutup Wiraputra, Muhammad Dwijunianto; Nurchayati, Yulita; Hastuti, Endah Dwi; Setiari, Nintya
Buletin Anatomi dan Fisiologi Volume 9, Nomor 1, Tahun 2024
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.9.1.2024.66-74

Abstract

Kualitas jahe merah ditentukan oleh kandungan minyak atsiri yang mudah menguap. Kombinasi cabinet solar dryer (CSD) sebagai alat pengering yang dikombinasikan dengan kain penutup dapat mempengaruhi rendemen minyak atsiri. Penelitian ini bertujuan untuk mempelajari pengaruh kombinasi metode pengeringan dengan penambahan kain penutup terhadap rendemen minyak atsiri jahe merah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2 x 3 dengan 3 ulangan. Faktor pertama adalah pengeringan dengan cabinet solar dryer dan metode terbuka tanpa alat. Faktor kedua adalah tanpa kain, 1 lapis kain dan 2 lapis kain penutup. Parameter yang diamati adalah volume minyak atsiri, rendemen minyak atsiri, kadar air, susut bobot, suhu pengeringan, kelembaban udara, dan morfologi jahe. Metode yang digunakan adalah pengeringan rimpang jahe, yang telah diiris dengan ketebalan 5 mm. Rendemen minyak atsiri dianalisis dengan metode destilasi uap dan air. Hasil penelitian menunjukkan terdapat interaksi antara metode pengeringan dan kain penutup terhadap rendemen minyak atsiri. Rendemen tertinggi (0,98%) diperoleh dengan metode pengeringan matahari langsung dengan 1 lapis kain penutup. Kadar air terendah dan susut bobot tertinggi terdapat pada perlakuan matahari langsung tanpa kain penutup sebesar 13,04% dan 79,93%. Perlakuan optimal dalam meningkatkan volume dan rendemen minyak atsiri diperoleh pada metode pengeringan matahari terbuka dengan 1 lapis kain penutup. Metode pengeringan dengan alat CSD tanpa kain penutup menghasilkan minyak atsiri yang tinggi sebesar 0,6%. The quality of red ginger is determined by its volatile essential oil content.  A cabinet solar dryer (CSD) combined with a cloth cover can affect the yield of ginger essential oil. This study aims the effect of the drying method combined with cover cloth on the yield of red ginger essential oil. This study used a completely randomized design (CRD) factorial pattern 2 x 3 with 3 repetitions. The parameters observed were essential oil volume and yield, water content, weight loss, drying temperature, drying humidity, and ginger morphology. The method used was dried rhizome chopped with a thickness of 5 mm. The essential oils were analyzed by steam and water distillation. The results showed that there was an interaction between the two treatments for essential oil yields. The highest essential oil yield (0.98%) was obtained from direct sunlight with 1 layer of cloth. The lowest water content and highest weight loss were found in direct sun without covering cloth at 13.04% and 79.93%. Optimal treatment in increasing oil volume and essential oil yield was obtained by drying in the open sun with 1 layer of cloth cover. Drying with a CSD without a cover cloth produces a high essential oil.  
Kandungan Vitamin C dan Morfometri Buah Jambu Kristal (Psidium guajava L. cv. ‘Kristal’) pada Pengemasan yang Berbeda Putra, Agita Christyaji; Nurchayati, Yulita; Hastuti, Endah Dwi; Setiari, Nintya
Buletin Anatomi dan Fisiologi Volume 8, Nomor 2, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.2.2023.146-153

Abstract

Jambu kristal (Psidium guajava L. cv. ‘Kristal’) memiliki daging buah berwarna putih, tekstur renyah, tidak memiliki banyak biji serta memiliki banyak kandungan gizi, salah satunya vitamin C. Buah jambu yang sudah dipanen umumnya dikemas sebelum didistribusikan dengan tujuan untuk meminimalisir kerusakan maupun degradasi kandungan vitamin C selama penyimpanan. Penelitian ini bertujuan untuk mengetahui pengaruh jenis pengemas yang berbeda terhadap kandungan vitamin C dan morfometri buah jambu kristal. Buah dipanen pada umur 100 HSA (Hari Setelah Antesis), berdasarkan rentang ukuran dan bobot yang sama. Buah yang telah dikemas kemudian disimpan dalam lemari pendingin pada suhu ±10oC selama 3 hari. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal dengan empat perlakuan, yaitu tanpa pengemas, plastik wrapping, besek, dan styrofoam. Tiap perlakuan dengan tiga ulangan. Analisis kandungan vitamin C dilakukan dengan metode spektrofotometri. Pengamatan morfometri dengan mengukur tingkat kekerasan buah, tingkat memar buah, dan warna kulit buah. Hasil penelitian menunjukkan bahwa kandungan vitamin C tertinggi terdapat pada jenis pengemas menggunakan styrofoam sebesar 12.297 ppm dan terendah pada perlakuan plastik wrapping sebesar 8.960 ppm. Perbedaan jenis pengemas berupa plastik wrapping, besek, dan styrofoam mempengaruhi kandungan vitamin C dan morfometri buah jambu kristal. Morfometri buah seperti susut diameter, susut bobot, tingkat kekerasan, dan tingkat memar buah yang paling baik diperoleh dari jenis pengemas styrofoam.  Crystal guava (Psidium guajava L. cv. 'Kristal') has white flesh, crunchy texture, low seed content and contains high vitamin C. Harvested guava are generally packaged before distribution to minimize degradation of vitamin C content during storage. This study aims to determine the effect of different types of packages on vitamin C content and morphometry of crystal guava fruit. Fruits are harvested at 100 days after anthesis based on the same size range and weight. The packed fruit was stored in the refrigerator at ±10oC for 3 days. The study was conducted using a single factor Completely Randomized Design (CRD) with four treatments, including without packaging, plastic wrap, besek (bamboo bucket), and styrofoam. Each treatment was repeated 3 times. Analysis of vitamin C content was carried out by spectrophotometric method. The results showed that the highest vitamin C content was found in the styrofoam treatment at 12,297 ppm and the lowest in the plastic wrap treatment at 8,960 ppm. Different types of packaging in the form of plastic wrap, besek, and styrofoam affect the vitamin C content and morphometry of crystal guava fruit. The best fruit morphometry such as diameter loss, weight loss, hardness level, and bruising level was obtained from styrofoam.
The Effect of Thidiazuron Treatment on the Growth of Vanda limbata Blume in vivo Ditasya Putri, Novika; Setiari, Nintya; Nurchayati, Yulita; Agung Suedy, Sri Widodo
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at three concentrations (factor levels) of 0, 25, and 50 ppm. Data were analyzed using analysis of variance and Duncan’s Multiple Range Test at 5% significance level. The study ran for three months and the following response variables were measure: the number of new leaves, increase in length of old leaves, length of new leaves, increase in width of old leaves, width of new leaves, number of roots, and stomatal density. The results showed that TDZ significantly increased leaf length and the number of new leaves of V. limbata, but decreased the density of stomata. Thidiazuron at 50 ppm was the best treatment for increasing the growth of V. limbata.
Thidiazuron-Induced Somatic Embryogenesis in Cymbidium bicolor Orchid In Vitro Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.15306

Abstract

Cymbidium bicolor is a highly hunted and traded orchid, leading to a decline in its wild population. Orchid conservation can be achieved through tissue culture, particularly via somatic embryogenesis. Thidiazuron (TDZ) is a growth regulator used to induce somatic embryogenesis. This study aimed to determine the optimal TDZ concentration for somatic embryo formation. Stem explants of C. bicolor were cultured on Murashige Skoog (MS) medium with TDZ concentrations of 0, 1, 2, and 3 ppm. Observations were conducted weekly for two months using a stereo microscope and OptiLab. Variables observed included the percentage of green explants, somatic embryo formation time, the number of explants forming somatic embryos, and the number and morphology of somatic embryos. The study was arranged in a Completely Randomized Design (CRD) with 14 replications. Results showed that TDZ addition influenced somatic embryo formation and maintained the green color of explants. Media with TDZ promoted faster growth and larger embryo size compared to media without TDZ. The optimal concentration was 1 ppm TDZ, which produced the highest number of embryos (172) and the fastest formation time compared to other concentrations (TDZ 0: 27, TDZ 2 ppm: 60, TDZ 3 ppm: 39).
Somatic Embryo Enhancing of Phalaenopsis amabilis (L.) Blume Orchid with 6-Benzyl Amino Purine (BAP) Karend, Azzah Hanun Abas; Setiari, Nintya; Nurchayati, Yulita; Rashid, Fatimah Azzahra Ahmad
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p88-98

Abstract

Phalaenopsis amabilis (L.) Blume is an orchid with high economic value because of its beautiful white flowers, yellow labellum, and long stems. Its existence in nature is threatened with extinction, so efforts are needed to cultivate and preserve P. amabilis, one of which is through somatic embryos, which generally require Plant Growth Regulators (PGRs) in the form of 6-Benzyl Amino Purine (BAP). 6-Benzyl Amino Purine (BAP) is used because it is one of the cytokinin hormones proven to induce somatic embryos. This study aims to examine and determine the use of the right BAP concentration to propagate P. amabilis through somatic embryo induction. The explants used were P. amabilis protocorms; because protocorms produced a greater number of somatic embryos than leaf explants, the development of the explants was observed every week for 2 months. This study used a single factor Completely Randomized Design (CRD) in the form of BAP concentrations of 0, 1, 2, 3, and 4 ppm with 20 replications. The results showed that the most optimal treatment for the propagation of P. amabilis somatic embryos was on media with an average number of somatic embryos at a concentration of BAP 3 ppm (65.00) embryo/explain.
Effect of Different Coconut Water Concentrations on the Growth of Dendrobium spectabile (Blume) Miq. Planlet on MS Media Santosa, Faradita Azzahra; Setiari, Nintya; Nurchayati, Yulita
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.533

Abstract

Dendrobium spectabile is an orchid with unique flowers that are vulnerable to exploitation. Tissue culture is employed to conserve this orchid, where the type of medium and organic supplements significantly affect plantlet growth. Murashige and Skoog (MS) media was added with coconut water as an exogenous hormone source. This study aimed to determine the effect of adding coconut water and determine the optimal concentration to increase the growth of D. spectabile at the subculture stage. The method involved subculturing D. spectabile plantlets in vitro in MS media supplemented with coconut water at concentrations of 0%, 5%, 10%, 15%, and 20%. The research design used a completely randomized design (CRD), with coconut water concentration and ten replicates. Data were analyzed using ANOVA and Duncan's Multiple Range Test at a 5% significance level. Plantlet growth was observed for two months, and the parameters observed were the emergence of new shoots, leaves, and roots; the number of new shoots, leaves, and roots; and the length of new leaves. The results showed that the addition of coconut water to the subculture medium can increase the leaf growth of D. spectabile orchid plantlets but does not increase shoot and root growth. Coconut water 10% was the most optimal concentration for the number of new leaves and new leaf length. The addition of 15% coconut water was the most optimal concentration for plantlet new leaf emergence. Coconut water plays a vital role in in vitro culture, supporting the various growth processes of D. spectabile plantlets. Keywords: Dendrobium spectabile, ex situ conservation, organic supplement, subculture
The Impact of Biochar and FeCl3 Administration on Fe Toxicity Symptoms and Productivity of Bok Choy Mustard Plants (Brassica rapa L.) Nauli Variety Prameswari, Ratna Apriliani; Nurchayati, Yulita; Setiari, Nintya
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.447

Abstract

Fe³⁺ pollution is a factor that reduces bok choy production. Its productivity can be increased by adding biochar, which can adsorb inorganic ions, including iron. This study examines the impact of rice husk biochar and FeCl₃ on bok choy productivity, using a completely randomized design with a 3´3 factorial pattern. The first factor was the concentration of rice husk biochar (0, 2.5, and 7.5 g/kg), and the second was the concentration of FeCl₃ (0, 25, and 50 mg/L). The criteria measured were the number of leaves, leaf area, chlorophyll content, and vitamin C levels. The combination of 0 g/kg biochar and 0 mg/L FeCl₃ proved to be the most effective treatment for boosting bok choy growth. The treatment yielded 12.6 leaf count, 73.3 cm² leaf area, 2.0 mg/g chlorophyll, and 30.8 ppm vitamin C. Fe³⁺ treatments at 25–50 mg/L drastically lowered all growth metrics. The applied biochar doses were insufficient to efficiently absorb Fe³⁺ and boost the yield of bok choy. Keywords: biochar, bok choy, chinese mustard, FeCl3, toxicity
The Effect of Benzyl Amino Purine (BAP) On Potato (Solanum tuberosum L.) Axillary Buds Micropropagation Putri Anugrah Maulidya; Nurchayati, Yulita; Setiari, Nintya
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 01 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss01/480

Abstract

Potato (Solanum tuberosum L.) production in Indonesia increased by 4.21% in 2022 compared to the previous year, but this production is still low by international standards. This is caused by low quality and quantity of potato seeds which is sourced from tubers. It is necessary to modify the in vitro cultivation of potato seed sources by utilising seeds that are often wasted with the addition of cytokinins, such as Benzyl Amino Purine (BAP). The aim of this study was to determine the effect of BAP on potato axillary shoot explants sourced from seeds. The study was conducted using a Completely Randomized Design with one factor, namely BAP concentrations of 0, 1, 2 and 3 ppm with 3 replications. The results showed that there is an influence of BAP on the parameters of shoot growth percentage, leaf and root emergence time, plant height, number of shoots, roots, and leaves of potato axillary buds planlet. The 2 ppm BAP treatment tends to give the best response in increasing the number of shoots with an average of 4.33 shoots, making it effective for producing potato seedlings from axillary buds in vitro.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Pertumbuhan Anggrek Cymbidium ensifolium (L.) Sw. dengan Pemberian Pupuk Hayati Mikoriza Misrofah, Sofatun; Misrofah, Sofatu; Setiari, Nintya; Nurchayati, Yulita; Suedy, Sri Widodo Agung
Jurnal Hortikultura Indonesia (JHI) Vol. 13 No. 1 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.1.35-42

Abstract

ABSTRAKAnggrek Cymbidium ensifolium adalah anggrek terestrial yang terdaftar di CITES 2020 sebagai spesies hampir terancam punah atau rentan sehingga perlu upaya pelestarian secara ex situ. Salah satu faktor pendukung budidaya anggrek adalah pemberian pupuk. Pupuk hayati mikoriza bersifat ramah lingkungan dan dapat mengurangi dampak negatif pupuk anorganik. Penelitian ini bertujuan untuk mengkaji pengaruh pupuk hayati mikoriza berbagai dosis terhadap pertumbuhan anggrek C. ensifolium. Penelitian ini disusun menggunakan Rancangan Acak Lengkap satu faktor, yaitu pupuk hayati mikoriza (0; 5; 10; 15 g tanaman-1) dengan 6 ulangan. Data dianalisis pada Analysis of Variance dengan taraf kepercayaan 95%. Percobaan dilakukan bulan Oktober 2021 – Februari 2022 di Tembalang, Semarang. Parameter yang diamati berupa waktu muncul tunas, jumlah tunas, waktu muncul daun, jumlah daun, dan persentase infeksi mikoriza. Hasil penelitian menunjukkan bahwa perlakuan pupuk hayati mikoriza berpengaruh signifikan terhadap waktu muncul tunas dan daun, jumlah daun, dan persentase infeksi mikoriza. Pupuk hayati mikoriza sebesar 5 g tanaman-1 merupakan perlakuan optimal untuk meningkatkan waktu muncul tunas dan daun, serta konsentrasi 15 g tanaman-1 optimal meningkatkan jumlah daun dan persentase infeksi mikoriza. Pengaruh pupuk hayati mikoriza ini dapat dikembangkan untuk pelestarian anggrek jenis lain. Kata kunci: anggrek spesies, Cymbidium, biofertilizer, mikoriza
Co-Authors Abdul Khafid, Abdul Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Della, Nelly Vikiladyla Desi Arisanti Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Eva Septiyana Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Hartati, Puji Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas Luaeliyah, Masrukhatul Misrofah, Sofatu Misrofah, Sofatun Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Prameswari, Ratna Apriliani Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Yulita Nurchayati Zakiyah, Aisyah