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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Planta Tropika Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Nintya Setiari
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health

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The Physiological Response of Germination and Growth in Solanaceae Plants (Capsicum frutescens, Solanum melongena, Solanum lycopersicum) at Different Salinity Levels Della, Nelly Vikiladyla; Hastuti, Endah Dwi; Setiari, Nintya
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 4 (2024): December 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i4.1352-1360

Abstract

High salinity causes osmotic stress and ion imbalance that can reduce plant productivity. Solanaceae can be developed for cultivation in saline land, but its growth is influenced by the type of species. This study aims to examine the tolerance level of three Solanaceae plants to salinity stress through observation of physiological responses of germination and growth. This study used a 3 x 4 factorial Completely Randomized Design (CRD). The first factor is salinity: 0 ppm, 2,500 ppm, 5,000 ppm and 7,500 ppm. The second factor is the Solanaceae species, namely Capsicum frutescens, Solanum melongena, and Solanum lycopersicum. Germination parameters include germination power, wet weight and dry weight. The growth parameters observed include plant height, root length, stem diameter, leaf area, number of leaves, wet weight of leaves, roots and stems and dry weight of leaves, roots and stems. The results of the study showed that C. frutescens is a plant that is more tolerant to salinity up to a concentration of 5,000 ppm when compared to S. melongena and S. lycopersicum whose tolerance is up to 2,500 ppm. Keywords: Germination, Salinity, Solanaceae, Vegetative Growth.
Pengaruh Media MS dan VW Terhadap Pertumbuhan Planlet Anggrek Bulan (Phalaenopsis amabilis L. Blume) Setelah Transplanting Istiqomah, Azura Muzdalifah; Setiari, Nintya; Nurchayati, Yulita
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.971 KB)

Abstract

Anggrek merupakan jenis tanaman hias yang memiliki bunga khas dengan mahkota indah dan warna menarik. Anggrek Bulan (Phalaenopsis amabilis) merupakan bagian dari bunga nasional dan dijuluki “Puspa Pesona”. Ketersediaan Anggrek Bulan di alam mulai berkurang, sehingga diperlukan upaya konservasi. Pembiakan anggrek alam dapat dilakukan dengan teknologi tissue culture atau kultur jaringan biji. Salah satu metode penting dalam kultur jaringan yaitu proses transplanting. Tujuan transplanting agar planlet anggrek tersebut tetap terjaga pertumbuhannya. Penggunaan media tumbuh yang tepatmerupakan salah satu faktor yang menentukan keberhasilan transplanting. Tujuan dari penelitian ini untuk mengetahuipertumbuhan Anggrek Bulan pada dua media berbeda, yaitu MS (Murashige and Skoog) dan VW (Vacin and Went). Metode yang digunakan adalah transplanting planlet Anggrek Bulan umur 1 tahun hasil kultur jaringan dari biji ke media perlakuan MS dan VW tanpa ZPT. Selanjutnya planlet tersebut dipelihara di ruang inkubasi dengan cahaya 1000 lux dan suhu 25℃.Parameter yang diamati yaitu pertambahan jumlah tunas, jumlah daun, panjang daun, lebar daun, dan panjang akar. Pengamatan dilakukan setiap seminggu sekali. Hasil pengamatan menunjukkan bahwa media MS meningkatkan jumlah tunas dan panjang akar, serta mempercepat munculnya tunas baru yaitu satu minggu setelah transplanting. Simpulan dari penelitian ini adalah pertumbuhan planlet cenderung lebih baik di media MS dibanding media VW.
Effect of Ammonium Sulfate on Flower and Pod Development of Soybean [Glycine max (L.) Merr.] Setiana, Devi Vira; Nurchayati, Yulita; Setiari, Nintya
Jurnal Ilmu Pertanian Indonesia Vol. 31 No. 1 (2026): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.31.1.17

Abstract

Soybean [Glycine max (L.) Merr.] is a food crop rich in vegetable oil and protein, with increasing demand and economic worth. Increasing soybean production can be accomplished by providing nutrients nitrogen and sulfur, hence increasing the percentage of bloom development into pods. Applying ammonium sulfate at the start of planting can fulfill nitrogen and sulfur fertilizer requirements. The purpose of this study was to examine and determine the ideal concentration of ammonium sulfate for optimal flower and pod formation, as well as the percentage of flower development into pods and leaf sulfur content. Ammonium sulfate was applied in an experimental garden by mixing it into the planting material in polybags under controlled settings. This study employed a single-factor fully randomized design with four doses of ammonium sulfate. The treatments were ammonium sulfate [NH4)2SO4] at various concentrations of 0, 100, 200, and 300 kg/ha administered at the start of planting, with five replications. The research factors were the quantity of flowers, pods, percentage of flower development into pods, and sulfur level in the leaves. The sulfur content of the leaves was determined using an atomic absorption spectrophotometer. Data were analyzed using ANOVA at a significance level of 5%, followed by DMRT if the results were significant. The results revealed that ammonium sulfate 300 kg/ha produced the highest number of flowers, pods, percentage of flower development into pods, and sulfur content in leaves. Keywords: ammonium sulfate, nitrogen, productivity, soybean, sulfur
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
THE GROWTH OF Dendrobium spectabile (Blume) Miq. ORCHID PLANTLET IN VACIN AND WENT (VW) MEDIUM WITH DIFFERENT CONCENTRATIONS OF COCONUT WATER Zakiyah, Aisyah; Setiari, Nintya; Nurchayati, Yulita
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.222

Abstract

Dendrobium spectabile is an epiphytic orchid that grows slowly and experiences natural exploitation, so it needs to be conserved ex-situ through in vitro culture. One of the essential steps for in vitro culture is subculture to supply the nutrients needed by plantlets. Increasing the growth of these plantlets can be done by adding coconut water. This study aimed to determine the effect of adding coconut water and the optimal concentration of coconut water on the growth of D. spectabile plantlets in VW medium. The method used is growing plantlets in VW medium with different concentrations of coconut water. The research design was completely randomized with a single factor, the concentration of coconut water (0%, 5%, 10%, 15%, 20%). Data were analyzed by ANOVA at a 5% significance level. Plantlet growth was observed for two months. Parameters observed were new shoots, leaves, and roots emergence time; number of new shoots, leaves, and roots; and new leaves length. The addition of coconut water affected the growth of D. spectabile orchid plantlets. Five to ten percent of coconut water is the optimal concentration for shoots and leaves growth of D. spectabile orchid plantlets. Therefore, coconut water can increase the growth of in vitro plantlets.
Pengaruh Air Kelapa Pada Media MS Terhadap Pertumbuhan Tunas Dari Eksplan Cormus Saffron (Crocus Sativus L.) Secara In vitro Utama, Rafii Satria; Setiari, Nintya; Nurchayati, Yulita; Haryanti, Sri
Buletin Anatomi dan Fisiologi Volume 11, Nomor 1, Tahun 2026
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.1.1.2026.1-8

Abstract

Saffron (Crocus sativus L.) merupakan tumbuhan steril dengan kromosom triploid, sehingga perbanyakan secara generatif tidak dapat dilakukan. Metode kultur jaringan menjadi salah satu alternatif untuk membudidayakan saffron. Tujuan penelitian adalah mengkaji pengaruh air kelapa pada media MS terhadap pertumbuhan tunas eksplan cormus Saffron  dan mengetahui konsentrasi air kelapa yang optimum sehingga meningkatan pertumbuhan tunas Saffron. Metode yaitu penanaman eksplan cormus Saffron  ke dalam media MS yang ditambah air kelapa pada konsentrasi 0%, 5%, 10%, 15%, 20%. Penelitian menggunakan rancangan acak lengkap dengan faktor tunggal (konsentrasi air kelapa) dengan 4 ulangan. Data dianalisis dengan ANOVA dilanjutkan dengan uji DMRT. Pertumbuhan eksplan diamati selama 8 minggu. Parameter yang diamati yaitu waktu muncul tunas, akar, dan daun; jumlah tunas, akar, dan daun. Hasil penelitian menunjukkan bahwa penambahan air kelapa tidak menginisiasi pertumbuhan akar, tunas, dan daun cormus saffron. Air kelapa dengan konsentrasi 0% - 20% tidak menstimulasi pertumbuhan akar, tunas, dan daun cormus saffron. Air kelapa belum mampu memecahkan dormansi cormus saffron.Saffron (Crocus sativus L.) is a sterile plant with triploid chromosomes, making generative propagation unfeasible. Tissue culture offers an alternative method for saffron cultivation. This research aimed to investigate the impact of coconut water in Murashige and Skoog (MS) medium on the growth of saffron corm explant shoots and to identify the optimal coconut water concentration for enhancing saffron shoot growth. The experiment involved planting saffron corm explants in MS medium supplemented with coconut water at concentrations of 0%, 5%, 10%, 15%, and 20%. A completely randomized design with a single factor (coconut water concentration) and four replications was employed. Data were analyzed using ANOVA followed by the Duncan's Multiple Range Test (DMRT). Explant growth was monitored over an 8-week period, with observations focusing on the timing of shoot, root, and leaf emergence, as well as the number of shoots, roots, and leaves. The results indicated that the addition of coconut water did not initiate the growth of saffron corm roots, shoots, or leaves. Coconut water at concentrations ranging from 0% to 20% did not stimulate the growth of saffron corm roots, shoots, or leaves, and it was unable to break the dormancy of saffron corms.
Co-Authors Abdul Khafid, Abdul Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Della, Nelly Vikiladyla Desi Arisanti Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Eva Septiyana Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Hartati, Puji Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas Luaeliyah, Masrukhatul Misrofah, Sofatu Misrofah, Sofatun Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Prameswari, Ratna Apriliani Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Utama, Rafii Satria Wahyu Hadayani Wiraputra, Muhammad Dwijunianto Yulita Nurchayati Zakiyah, Aisyah