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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Cancer Chemoprevention Seminar Nasional Teknik Kimia Kejuangan INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Indonesian Journal of Chemistry International Journal of Community Service Learning JFIOnline Jurnal Jamu Indonesia Patria : Jurnal Pengabdian Kepada Masyarakat International Journal of Community Service Abdimas Altruis: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Jamu

Agustina Setiawati

Faculty Of Pharmacy, Universitas Sanata Dharma, Yogyakarta

Author-ID : 377205

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual Setiawati, Agustina
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 7, No 3 (2015)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Resistensi antibiotik masih menjadi perhatian dalam pengobatan penyakit infeksi. Penggunaan antibiotik yang tidak sesuai meningkatkan kasus terjadinya resistensi antibiotik. Penelitian ini didesain untuk membuat metode yang dapat digunakan untuk membuat kultur Staphycoccus aureus (S.aureus) yang resisten terhadap amoxicillin. Selanjutnya, bakteri tersebut dapat digunakan sebagai model bakteri resisten adaptif dalam penelitian mikrobiologi. Penelitian ini menggunakan metode adaptif gradual dengan sub kultur bakteri pada media mengandung amoxicillin sub-MIC. Konsentrasi sub-MIC ditingkatkan setiap minggu selama tiga minggu. MIC amoxicillin diuji kembali setiap akhir sub kultur sub-MIC pada satu konsentrasi. Amoxicillin bersifat bakterisidal terhadap S.aureus dengan MIC 0,25µg/mL. Konsentrasi amoxicillin sub MIC yang digunakan adalah 0,10; 0,15 dan 0,20 µg/mL. Metode ini berhasil membuat S.aureus resisten dengan meningkatkan MIC 300x pada akhir sub kultur 0,10 µg/mL dan meningkatkan MIC 400x pada akhir sub kultur 0,15 µg/mL MIC tidak meningkat. Metode adaptif gradual berhasil meningkatkan resistensi S.aureus terhadap amoxicillin.

Peningkatan Resistensi Kultur Bakteri Staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual Setiawati, Agustina
Jurnal Farmasi Indonesia Vol 7, No 3 (2015)
Publisher : Jurnal Farmasi Indonesia

Resistensi antibiotik masih menjadi perhatian dalam pengobatan penyakit infeksi. Penggunaan antibiotik yang tidak sesuai meningkatkan kasus terjadinya resistensi antibiotik. Penelitian ini didesain untuk membuat metode yang dapat digunakan untuk membuat kultur Staphycoccus aureus (S.aureus) yang resisten terhadap amoxicillin. Selanjutnya, bakteri tersebut dapat digunakan sebagai model bakteri resisten adaptif dalam penelitian mikrobiologi. Penelitian ini menggunakan metode adaptif gradual dengan sub kultur bakteri pada media mengandung amoxicillin sub-MIC. Konsentrasi sub-MIC ditingkatkan setiap minggu selama tiga minggu. MIC amoxicillin diuji kembali setiap akhir sub kultur sub-MIC pada satu konsentrasi. Amoxicillin bersifat bakterisidal terhadap S.aureus dengan MIC 0,25Âµg/mL. Konsentrasi amoxicillin sub MIC yang digunakan adalah 0,10; 0,15 dan 0,20 Âµg/mL. Metode ini berhasil membuat S.aureus resisten dengan meningkatkan MIC 300x pada akhir sub kultur 0,10 Âµg/mL Â dan meningkatkan MIC 400x pada akhir sub kultur 0,15 Âµg/mL MIC tidak meningkat. Metode adaptif gradual berhasil meningkatkan resistensi S.aureus terhadap amoxicillin.

Cytotoxic of Ganoderma lucidum in Colon Cancer through Cyclooxygenase 2 (COX-2) as Its Molecular Target Setiawati, Agustina
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.14

Many studies were designed explore chemopreventive activity of natural products on colon cancer especially addressing COX-2 as molecular target. Another promising source of natural product that potentially exhibit anticancer activity on colon cancer is Ganoderma lucidum. This study assessed selectivity of cytotoxic effect of G. lucidum extract on WiDr to Vero cells and investigated molecular mechanism on COX-2. G. lucidum ex-tract was prepared by reflux extraction method; in vitro anticancer was assayed by MTT method on WiDr and Vero cell line. This study applied apoptosis induction assay to observe cell death mechanism using double staining method; further COX-2 expression was stained by immunocytochemistry method. G. lucidum extract has cytotoxic effect on WiDr cells with IC50 135 Âµg/mL. However, the cytotoxic effect had low selectivity to-wards Vero cells with Selectivity Index (SI) 3.66. The extract induced apoptosis and suppressed COX-2 ex-pression in WiDr cells. G. lucidum extract was potential to be developed as anticancer agent towards colon cancer.

ANTICANCER ACTIVITY OF MANGOSTEEN PERICARP DRY EXTRACT AGAINST MCF-7 BREAST CANCER CELL LINE THROUGH ESTROGEN RECEPTOR -α Setiawati, Agustina
Indonesian Journal of Pharmacy Vol 25 No 3, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Breast cancer has very complex morphological and molecular characteristic. Estrogen receptor is one of biomarker in breast cancer progression, more than 60% breast cancer overexpress estrogen receptor α (ERα). Xanthone in Garcinia mangostana was investigated whether to have anticancer activity on colorectal, prostate, lung, blood and breast cancer. This research was focused on molecular mechanism of anticancer activity of mangosteen pericarp extract (MPE) on ER-α. This study used MCF-7 cells as a model of ER-α overexpressed breast cancer cells. Cytotoxic study towards MCF-7 cells was designed by using MTT test, further apoptotic induction assay was determined by double staining method using acridine orange and ethidium bromide. Extract molecular mechanism against breast cancer was assayed by immunocytochemistry. The MTT data was analyze using probit analysis to get IC50 then apoptosis and immunocytochemistry data were analysis qualitative analysis. MPE had strong cytotoxic activity on MCF-7 cells with IC50 of 45μg/mL and morphological changes passed through apoptosis induction. The expression of ER-α in MPE treated cells was same as untreated cells. MPE did not suppress ER-α in both nucleus and cytoplasm. Anticancer activity of MPE misht be mediated by other gene involved in ER-α signaling pathway in breast cancer cells.

SUATU KAJIAN MOLEKULER KETERGANTUNGAN NIKOTIN Agustina Setiawati
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 10, No 2 (2013)
Publisher : Sanata Dharma University

Abstract: Nicotine, a simple chemical substance from tobacco, stimulates addiction byneuroadaptation inducing rewards system in human brain. Molecular mechanism of nicotineaddiction in rewards system are mediated by nicotinic acetylcholine receptor (nAChR) andactivate dopaminergic neuron. Thus, nicotine addiction are also induce other neurottransmitterand hormone release: norephinefrin, serotonin, opioid, glutamat and mono amine oxidase.Keywords: nicotine, addiction, rewards system.

ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI Agustina Setiawati; Sri Hartati Yuliani; Enade Perdana Istyastono; Michael Raharja Gani; Evy Fenny Veronica; Dina Christin Ayuning Putri; Reza Eka Putra; David Chandra Putra; Agnes Mutiara Kurniawan
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 11, No 1 (2014)
Publisher : Sanata Dharma University

Rapid and simple quantitative analysis isoflavones tempe using densitometric TLChas been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5:0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distanceof 1 ?L sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linearrelationship obtained at 0.08 - 2 ?g/spot with r= 0.9986. The LOD and LOQ of isoflavone were0.014 ?g/spot and 0.048 ?g/spot. Genistein contained in tempe was 0.151 0.005 % b/b.

Retrospective Validation of a Structure-Based Virtual Screening Protocol to Identify Ligands for Estrogen Receptor Alpha and Its Application to Identify the Alpha-Mangostin Binding Pose Agustina Setiawati; Florentinus Dika Octa Riswanto; Sri Hartati Yuliani; Enade Perdana Istyastono
Indonesian Journal of Chemistry Vol 14, No 2 (2014)
Publisher : Universitas Gadjah Mada

The publicly available enhanced data of ligands and decoys for estrogen receptor alpha (ERα) which were recently published has made the retrospective validation of a structure-based virtual screening (SBVS) protocol to identify ligands for ERα possible. In this article, we present the retrospective validation of an SBVS protocol using PLANTS molecular docking software version 1.2 (PLANTS1.2) as the backbone software. The protocol shows better enrichment factor at 1% false positives (EF1%) value and the Area Under Curve (AUC) value of the Receiver Operator Characteristic (ROC) compared to the original published protocol. Moreover, in all 1000 iterative attempts the protocol could reproduce the co-crystal pose of 4-hydroxitamoxifen in ERα binding pocket. It shows that the protocol is not only able to identify potent ligands for ERα but also able to be employed in examining binding pose of known ligand. Thence, the protocol was successfully employed to examine the binding poses of α-mangostin, an ERα ligand found in the Garcinia mangostana, L. pericarp.

EFEK SITOTOKSIK EKSTRAK DIETIL ETER HERBA PEGAGAN EMBUN (Hydrocotyle sibthorpioides Lmk.) TERHADAP SEL KANKER PAYUDARA MCF-7 Agustina Setiawati; Yohanes Dwiatmaka
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 12, No 1 (2015)
Publisher : Sanata Dharma University

Abstract: Triterpenoid saponin, hydrocosaponin A-F, isolated compound from Hydrocotyle sibthorpioides Lmk. performed cytotoxic activity on KB, Daoy and WiDr cancer cells. Methanolic extract of this herb was also investigated in inhibiting cancer development on liver and cervical cancer. Therefore, it is potential to be developed as chemopreventive agent. The objective of this study is to determine cytotoxic activity and compound in diethyl ether extract of Hydrocotyle sibthorpioides Lmk. herbs. The cytotoxic effect of extract on MCF-7 breast cancer cell line was assayed using MTT method while phytochemical analysis was observed using normal phase Thin Layer Chromatoraphy (TLC). Diethyl ether extract of Hydrocotyle sibthorpioides had cytotoxic effect on MCF-7 cells with IC50 365 g/mL. TLC analysis observed that the extract contain flavonoid compounds. Keywords : Hydrocotyle sibthorpioides, MCF-7, cytotoxic, flavonoid

Pembelajaran Siswa Sekolah Dasar dengan Bumbu Dapur Sebagai Obat Tradisional yang Manjur dwiatmaka, yohanes; Wulandari, Erna Tri; Setiawati, Agustina; Hartinni, Yustina Sri; Permatasari, Olga Sancaya Dyah; Widiyanto, Yohanes Baptista Cahya
Patria : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 2: September 2024
Publisher : Universitas Katolik Soegijapranata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24167/patria.v6i2.11155

This community service target were class VI students at Kanisius Duwet Elementary School (SDK Duwet), Sleman, Yogyakarta. The team consists of lecturers from the Department of Pharmacy and Psychology at Sanata Dharma University (SDU), assisted by several students. Implementation of Phase-1 for the introduction of spices and herbal plants: garlic, shallots, ginger, turmeric, and galangal. Phase-1 was carried out with a direct visit to the Medicinal Plant Garden Laboratory, Faculty of Pharmacy, SDU. Stage-2 activities were carried out at SDK Duwet, carried out in the form of practical training in making and using several traditional medicines (TM) from kitchen spices that had been introduced in stage-1. Student understanding was measured through pretest and posttest. The result of the phase-1 pretest was very good (mean 90.53). Only a few students (23.68%) did not know galangal and turmeric. In phase-2 the mean pretest score was 60.53, lower than in phase-1. This showed that students knew the names of spices, but did not know their benefits as TM. Phase-2 posttest results with a mean of 89.71. According to the t-test between posttest and pretest in phase-2, students' understanding significantly increased by 148% (P0.01). This service program was successful in improving students' understanding and skills. The activity was closed with a group presentation. During the group presentation, students were enthusiastic in their delivery and stated that they were very impressed and amazed by this new knowledge about traditional medicines.

Edukasi Pengenalan, Pemanfaatan dan Pengelolaan Tanaman Obat pada Siswa Kelas VI Sekolah Dasar Setiawati, Agustina
Patria : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 1: Maret 2024
Publisher : Universitas Katolik Soegijapranata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24167/patria.v6i1.10570

Pemanfaatan tanaman obat telah dikenal secara empiris dapat membantu mengobati dan meringankan gejala penyakit di masyarakat Indonesia. Pemanfaatan ini belum optimal disebabkan oleh karena kurangnya edukasi dan pengenalan tanaman obat. Pada kegiatan ini, dilakukan edukasi pada siswa SD kelas VI sejumlah 38 anak. Edukasi ini dilakukan secara pemaparan materi mengenai tanaman obat Indonesia dan pengelolaannya, dilanjutkan dengan pembelajaran langsung dengan mengunjungi kebun obat, dan de-briefing peneguhan materi yang didapatkan oleh para siswa. Selain itu, para siswa diperkenalkan proses pembuatan minuman herbal dari bunga telang. Evaluasi keberhasilan kegiatan ini dilakukan dengan peningkatan skor test, yaitu 32,37 ± 13.24 sebelum edukasi, menjadi 66,05 ± 21,50 setelah edukasi. Dengan demikian, kegiatan ini berhasil meningkatkan pengetahuan para siswa mengenai tanaman obat Indonesia.

Co-Authors Adam Hermawan Aditya Fitriasari Agnes Mutiara Kurniawan Cahyaning Ratri, Monica David Chandra Putra Dina Christin Ayuning Putri Dita Maria Virginia Edy Meiyanto Enade Perdana Istyastono Enade Perdana Istyastono Erna Tri Wulandari Evy Fenny Veronica Flavia Domitilla Erika Setyajati Florentinus Dika Octa Riswanto Gelu, Maria Andriani Hanindita, Fizkha Hartinni, Yustina Sri Husin, Amelia S Ivana, Cheryn Kencana, Nyoman Bayu Wisnu Kinanti, Valentina Putri Embun Krisnina Prasetyo, Vincentia Lutfi Chabib, Lutfi Michael Raharja Gani Olga Sancaya Dyah Permatasari Ratna Asmah Susidarti reza eka putra Rini Dwiastuti Roni Permana Saputra Sabatti, P. Henrietta Puji Dwi Astuti Dian Saputra, Bakti Wahyu Sendang Husin, Amelia Sendy Junedi Setyajati, F.D. Erika Sri Hartati Yuliani Widiyanto, Yohanes Baptista Cahya Yohanes Dwiatmaka Yunita Linawati Yustina Sri Hartini

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