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Metode Ekstraksi dan Karakterisasi Kitosan Dari Limbah Seafood : Kajian Pustaka Ivana, Cheryn; Husin, Amelia S; Setiawati, Agustina
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

Show Abstract | Download Original | Original Source | Check in Google Scholar

Seafood waste is one of the common sources for chitosan production, such as crustaceans (shrimp, crab, and sea shells), squid cartilage, and others. Chitosan is a cationic biodegradable polymer which is biocompatible and has nontoxic properties to be applied in biological system. Chitosan, degraded from chitin, widely is used for drug delivery, tissue engineering (bone scaffold), food (coating film), agriculture (protection from harmful microorganisms), and pharmacological therapy such as an antibacterial agent for the human body. Chitosan is extracted with various methods such as chemical extraction, biological extraction, and microwave radiation extraction. Tthe characterization of chitosan can be known by percent yield, organoleptic test, dissolution, water content, and FTIR spectroscopy. The purpose of this review article is to overview the chitosan extraction method from environmental waste including the advantages and disadvantages of each method.

Consumer Profiles and Drink Menus Made from Medicinal Plants at the Jamu Cafe in Depok Sleman District, Yogyakarta Hartini, Yustina Sri; Setiawati, Agustina; Dwiatmaka, Yohanes
Jurnal Jamu Indonesia Vol. 9 No. 2 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i2.310

The use of medicinal plants as ingredients in making drinks in cafes is increasing along with the number of cafes. Herbal café makes a unique menu using medicinal plants in drink recipes to produce a distinctive taste, color, and aroma. This research aims to identify visitor profiles and drink menus made from medicinal plants and explore the understanding of visitors and cafe owners regarding the benefits of medicinal plants added to drinks. The research data collection technique uses a questionnaire equipped with an interview sheet. The questionnaires and interview sheets used have been tested for language comprehension and validity. The research was conducted at 272 cafes in Depok District, Sleman Regency, Special Region of Yogyakarta. The sample was determined using purposive sampling, data was collected after obtaining ethical clearance approval from the Health Research Ethics Committee Team. Data was obtained through filling out surveys by visitors and cafe owners, complemented by interviews with cafe owners. Data was collected three times: August, September, and October 2023. Data analysis was carried out descriptively and qualitatively. Most herbal cafe visitors (70%) are aged between 21–30 years with the majority of professions (44%) being students. The source of information about herbal cafe menus was mostly obtained (56%) from friends or family, and the frequency of visits to cafes was mostly (82%) between 1–5 times per month. More than 98% of visitors stated the benefits of medicinal plants in the drink menu, namely as a refresher or body warmer. There are 14 types of medicinal plants used as ingredients in the herbal cafe's drink menu, with a total monthly requirement for all medicinal plants of 545.1 kilograms; ginger ranks among the highest requirements. Recipe information for the menu was obtained from the family, the Ministry of Health Republic Indonesia website, and the herbal cafe owner made the recipe independently.

EDUKASI PENGELOLAAN OBAT SECARA MANDIRI DI PAROKI WARAK Linawati, Yunita; Setiawati, Agustina; Sabatti, P. Henrietta Puji Dwi Astuti Dian
ABDIMAS ALTRUIS: Jurnal Pengabdian Kepada Masyarakat Vol 7, No 2 (2024): Oktober 2024
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/aa.v7i2.9452

Self-medication is a community effort to choose treatment and medication to deal with complaints or symptoms of disease before seeking help from health facilities or medical personnel. There is a need and interest among Warak Parishioners regarding education on independent drug management at the household level because they do not yet have basic drug knowledge regarding drug categories and how to obtain, store, and dispose of drugs properly and correctly. This activity aims to provide education to people so that they are able to recognize types of medicines, how to get them, and how to store medicines properly, including regarding the period of use of medicinal preparations, and how to dispose of medicinal preparations properly and correctly. This activity is expected to improve the ability of Warak Parishioners to obtain, use, store, and dispose of medicine properly and correctly. The service activities began with attendance, distribution of goodie bags, pretest (10 minutes), education (60 minutes), question and answer (15 minutes), and posttest (10 minutes). The 38 participants consisted of the majority of women, aged 56-65 years and most of them had a high school education. The education provided could increase the knowledge of the Warak Parish community regarding independent, good, and correct medication management.

An Early Exposure of Over-the-Counter Medicine Basic Knowledge among Elementary School Students: Over-the-Counter, knowledge, students Hanindita, Fizkha; Kencana, Nyoman Bayu Wisnu; Flavia Domitilla Erika Setyajati; Setiawati, Agustina
International Journal of Community Service Learning Vol. 7 No. 3 (2023): Agustus
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v7i3.57752

Recently, self-medication using over-the-counter medicine (OTC) or non-prescription medicine became rapidly popular. Enhancing society's awareness of OTC knowledge and its rational use is greatly significant to promote public health both in adults and children. In the US, a high number of young children are brought to the emergency room due to accidental unsupervised medication each year. Therefore, learning how to self-medicate using OTC at an early age is very critical to improve medication safety in society. This study aims to trained elementary school grades V and IV at the age of 10 to 12 to perceive the basic knowledge of OTC and how to take them safely. This study conducted outreach to students in grades V and VI starting with a pre-test, education dan training, followed by a question-and-answer session, and closed with a post-test. After education and training, the knowledge of the OTC among the students increased, especially significantly in Grade V indicated by post-test score elevating. Thus, this attempt may contribute to increasing the awareness of self-medication using OTC among children.

Formulation of Nanoemulsion Sunscreen of Centella asiatica (L.) Extract and Its Effectivity Test Dwiastuti, Rini; Kinanti, Valentina Putri Embun; Gelu, Maria Andriani; Setiawati, Agustina; Chabib, Lutfi
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 1 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.009910

Sunscreen protects against ultraviolet (UV) rays. It has a Sun Protection Factor (SPF) value. Centella asiatica (L.) is a herbal plant that contains flavonoid and triterpenoid compounds as antiphotoaging agents. Development of Centella asiatica (L.) extract in nanoemulsion preparation increases its solubility in water and absorption. This study aims to obtain the optimum formula of Centella asiatica (L.) nanoemulsion preparation from the combined use of Tween-80 and Propylen glycol surfactants and determine the effectiveness of Centella asiatica (L.) extract in vitro. The effectiveness of Centella asiatica (L.) extract was tested in vitro using UV-Vis spectrophotometry expressed by SPF value. Development of Centella asiatica (L.) extract nanoemulsion formula using a combination of surfactants Tween-80 and Propylen glycol with factorial design 2 factors and 2 levels. P-value0.05 stated that there is a statistically significant difference in the formula. Centella asiatica (L.) extract has protective power against UV rays at concentrations of 120, 240, and 360 xg with SPF-values of 8.999, 19.219, and 29.569, respectively. Meanwhile, the nanoemulsion response analysis revealed the response of the nanoemulsion produced (p0.05). The optimum formula obtained was found in the nanoemulsion formula with a combination of Tween-80 and PG at a low level.

Edible Cell Model For Cell Biology Learning For The Elementary School Students Sendang Husin, Amelia; Setyajati, F.D. Erika; Krisnina Prasetyo, Vincentia; Saputra, Bakti Wahyu; Cahyaning Ratri, Monica; Setiawati, Agustina
International Journal Of Community Service Vol. 2 No. 3 (2022): August 2022 (Indonesia - Malaysia - Kamboja)
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijcs.v2i3.122

Learning cell biology in science subject is a challenging for elementary students in Indonesia. Not only it is implicitly taught in the science subject, but it also needs an intangible imagination to understand the cell’s organelles and their function. Therefore, a recreating method to explain about the cells the cell’s organelles and their function. In our program, the 5th and 6th of elementary students were explained about cells, organelles and their function in the first week class. Thus, they also were taught to observe cells under light microscope. Moreover, we developed the cells model by creating it from the edible materials such as cake, candies, messes, and biscuits; followed by fun game. While the cognitive ability of the students was evaluated with pre-and post-test. Our method increased the level of understanding of the students to learn cell biology. However, the standard deviation of student cognitive ability was highly varied in both classes.

The Doxorubicin-Induced G2/M Arrest in Breast Cancer Cells Modulated by Natural Compounds Naringenin and Hesperidin Junedi, Sendy; Hermawan, Adam; Fitriasari, Aditya; Setiawati, Agustina; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 12, No 2 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss2pp83-89

Doxorubicin as the common drug for breast cancer has been widely proposed to use in combine with a natural compound in order to overcome its side effects such as cardiotoxicity and resistance. Previously, we reported that naringenin and hesperidin, the abundant flavanons in citrus fruit peel, increased cytotoxic and apoptosis activities of doxorubicin in doxorubicin resistant breast cancer cells (T47D and MCF-7 cells). Since doxorubicin arrests G2/M phase in most cancer cells, both flavanons are speculated to affect the similar phase in breast cancer cells. Cell cycle distributions were determined by flowcytometry using propidium iodide (PI) to stain DNA of the cells. Combination of naringenin or hesperidin with doxorubicin increased accumulation of T47D cells in G2/M phase, while in MCF-7 cells, accumulated cells in G2/M phase were decreased, accompanying with slightly increased in G1 phase. Naringenin itself had no effect on cell cycle of both cells. Whereas, hesperidin arrested G2/M and G1 phases in T47D and MCF-7 cells, respectively. The different effect of naringenin and hesperidin in T47D and MCF-7 cells is most likely caused by difference of p53 status. In p53 mutant, T47D cells, naringenin and hesperidin supported mechanism of doxorubicin to arrest at G2/M that to be considered via p53-independent pathway. Whereas, in p53 wild-type MCF-7 cells, naringenin and hesperidin decreased G2/M arrest, suggesting that both flavanons do not utilize cell cycle arrest for their anticancer activity with doxorucibin. This study revealed that potential co-chemoterapeutic agents, naringenin and hesperidin distinctly modulated cell cycle arrest induced by doxorubicin according to the characteristic of breast cancer cells.Keywords: naringenin, hesperidin, doxorubicin, cell cycle, breast cancer cells.

Consumer Profiles and Drink Menus Made from Medicinal Plants at the Jamu Cafe in Depok Sleman District, Yogyakarta Hartini, Yustina Sri; Setiawati, Agustina; Dwiatmaka, Yohanes
Jurnal Jamu Indonesia Vol. 9 No. 2 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i2.310

The use of medicinal plants as ingredients in making drinks in cafes is increasing along with the number of cafes. Herbal café makes a unique menu using medicinal plants in drink recipes to produce a distinctive taste, color, and aroma. This research aims to identify visitor profiles and drink menus made from medicinal plants and explore the understanding of visitors and cafe owners regarding the benefits of medicinal plants added to drinks. The research data collection technique uses a questionnaire equipped with an interview sheet. The questionnaires and interview sheets used have been tested for language comprehension and validity. The research was conducted at 272 cafes in Depok District, Sleman Regency, Special Region of Yogyakarta. The sample was determined using purposive sampling, data was collected after obtaining ethical clearance approval from the Health Research Ethics Committee Team. Data was obtained through filling out surveys by visitors and cafe owners, complemented by interviews with cafe owners. Data was collected three times: August, September, and October 2023. Data analysis was carried out descriptively and qualitatively. Most herbal cafe visitors (70%) are aged between 21–30 years with the majority of professions (44%) being students. The source of information about herbal cafe menus was mostly obtained (56%) from friends or family, and the frequency of visits to cafes was mostly (82%) between 1–5 times per month. More than 98% of visitors stated the benefits of medicinal plants in the drink menu, namely as a refresher or body warmer. There are 14 types of medicinal plants used as ingredients in the herbal cafe's drink menu, with a total monthly requirement for all medicinal plants of 545.1 kilograms; ginger ranks among the highest requirements. Recipe information for the menu was obtained from the family, the Ministry of Health Republic Indonesia website, and the herbal cafe owner made the recipe independently.

Metode Ekstraksi dan Karakterisasi Kitosan Dari Limbah Seafood : Kajian Pustaka Ivana, Cheryn; Husin, Amelia S; Setiawati, Agustina
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

Show Abstract | Download Original | Original Source | Check in Google Scholar

Seafood waste is one of the common sources for chitosan production, such as crustaceans (shrimp, crab, and sea shells), squid cartilage, and others. Chitosan is a cationic biodegradable polymer which is biocompatible and has nontoxic properties to be applied in biological system. Chitosan, degraded from chitin, widely is used for drug delivery, tissue engineering (bone scaffold), food (coating film), agriculture (protection from harmful microorganisms), and pharmacological therapy such as an antibacterial agent for the human body. Chitosan is extracted with various methods such as chemical extraction, biological extraction, and microwave radiation extraction. Tthe characterization of chitosan can be known by percent yield, organoleptic test, dissolution, water content, and FTIR spectroscopy. The purpose of this review article is to overview the chitosan extraction method from environmental waste including the advantages and disadvantages of each method.

Cytotoxic Activity of Anredera cordifolia Leaf Extract on Hela Cells Virginia, Dita Maria; Saputra, Roni Permana; Setiawati, Agustina
BIOTROPIA Vol. 26 No. 1 (2019): BIOTROPIA Vol. 26 No. 1 April 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.1.863

Cervical cancer is the second most frequently occurring cancer among females worldwide. Since the best strategy against cancer is to kill cancer cells without endangering normal cells, discovering a potentially selective anticancer agent from plants has become a major focus for researchers globally. A previous study on Anredera cordifolia, known as binahong in Indonesia, revealed its cytotoxic activity on HeLa cervical cancer cells with an IC₅₀ value of 75 µg/mL. However, the selectivity of the chemical agent and its molecular target was not investigated. Therefore, this study aimed to determine the selectivity of ethanolic extract of Anredera cordifolia leaf (EAC) on Vero cells and its molecular target on HeLa cells. The extract was prepared by macerating A. cordifolia leaf powder in 70% ethanol. The viability of Vero cells was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, while the cell cycle of HeLa cells was analyzed using flow cytometry. The molecular target of the extract was further investigated using immunocytochemical staining. The results demonstrated the selective cytotoxicity of EAC against HeLa cells compared to Vero cells, with a Selectivity Index (SI) of 17.36. The extract induced cell cycle arrest at the G1/S phase and suppressed Bcl-2 expression, an anti-apoptotic protein that also regulates cell cycle progression. These findings support the potential use of A. cordifolia leaf extract as a promising anticancer agent against cervical cancer, particularly HeLa cells.

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