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All Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions Journal of Health Sciences INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal) Jurnal Pengabdian Kesehatan Beguai Jejama
Suliati Suliati
Poltekkes Kemenkes Surabaya
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli Endah Prayekti; Suliati Suliati; Dwi Agustin Wulandari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1906

Abstract

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p < 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.
Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum Widyanur; Retno Sasongkowati; Suliati
Jurnal Ilmiah Kesehatan Vol 16 No 01 (2023): Jurnal Ilmiah Kesehatan (Journal of Health Science) 
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/jhs.v16i01.3181

Abstract

Green beans are cultivated plants and crops with nutrients from vegetable protein. Varieties of mung beans vary, including Vima 1 and local. The fungus Trichophyton rubrum generates dermatophytosis. This research explored green beans and local Vima 1 as an alternative medium in the growth of Trichophyton rubrum fungus. The type of research used by laboratory experiments was carried out at the STIKes Hutama Abdi Husada Tulungagung Microbiology Laboratory in March - April 2022. SDA media and alternative media green beans Vima 1 and local variations of the mass of 6 grams and 7 grams inoculated Trichophyton rubrum fungus using a single dot method with repetition as much as five times. The results showed that Trichophyton rubrum fungus grows optimally on alternative media green beans Vima 1 variation of 7 grams of mass that grows colonies on the fifth day with an average final diameter of 14 mm. While alternative media green beans Vima 1 variation mass 6 grams 11 mm, SDA media 11.65 mm, alternative media local green beans variation mass 6 grams 7.5 mm, and variation mass 7 grams 11.5 mm. The result of independent statistical tests was a p-value < 0.00, which shows significant differences in each type of media. This study result concluded that green beans Vima 1 and local could be used as an alternative medium to replace the SDA media in the growth of Trichophyton rubrum fungus.
Analysis of Mec-A Gene on Methicillin Resistant Staphylococcus aureus Suliati Suliati; Ayu Puspitasari; Anita Dwi Anggraini
Health Notions Vol 7, No 1 (2023): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn70105

Abstract

The prevalence of MRSA in various hospitals in the world ranges from 2-70% with an average of 20% .In recent decades there has been an increasing prevalence of S. aureus and MRSA in the world. Population-based studies in North America and Europe indicate the prevalence of S. aureus is between 18-30%. The overall prevalence of MRSA in Asia has reached 70%, while publications and prevalence of MRSA in Indonesia are still very limited and very difficult to obtain. The prevalence of MRSA in Atmajaya Hospital, Jakarta, reached 47%. Yuwono also reported in 2010 MRSA at Dr. RSUP. Moh. Hoesin Palembang reached 46%. Amplification were carried out under PCR conditions as a result of optimization, namely the results of PCR optimization were carried out for 30 cycles with the following thermal conditions. Denaturation phase at 94oC for 30 seconds, annealing at 52.0oC for 30 seconds, and extension at 72oC for 1 minute. Amplification results showed that of the 30 samples only 90% (27 samples) were detected to have the Mec-A gene, 10% (3 samples) were negative. Keywords: Mec-A; gene; Methicillin Resistant Staphylococcus aureus
Peningkatan Kemandirian Siswa dalam Menerapkan Pola Hidup Bersih dan Sehat untuk Mencegah dan Mengurangi Infeksi Soil Transmitted Helminths (Sth) di SD MI Sunan Ampel 1 Sidorogo-Trosobo Kecamatan Taman Kabupaten Sidoarjo Suliati; Wisnu Istanto; Anita Dwi Anggraini
ABDIKESMAS MULAWARMAN : Jurnal Pengabdian Kepada Masyarakat Vol.3 No.1 Mei (2023) : ABDIKESMAS MULAWARMAN
Publisher : Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Soil Transmitted Helminths (STH) adalah golongan cacing usus (Nematoda Usus) dalam perkembanganya membutuhkan tanah untuk men jadi bentuk infektif. Golongan STH yang habitatnya pada usus manusia adalah Ascaris lumbricoides, Hookworm (Necator americanus dan Ancylostoma duodenale), Strongiloides stercoralis, Trichuris trichiura. Madrasah Ibtidaiyah Sunan Ampel II mulai kiprahnya dalam dunia pendidikan sejak tahun1988 atas prakarsa beberapa tokoh agama, tokoh masyar akat dan adanya tuntutan dari masyarakat setempat. Mengingat semakin banyaknya output yang dihasilkan. Madrasah Ibtidaiyah Sunan Ampel II setiap tahunnya, juga kondisi masyarakat setempat yang tergolong mene ngah kebawah, sedangkan letak desa tersebut jauh dari lembaga pendidikan SMP dan setingkatnya yang ada disekitar desa tersebut. Penelitian dilakukan oleh Megadkk tahun 2020 di SD MI Sunan Ampel 1 Desa Sidorogo Kecamatan Taman Kabupaten di didapatkan hasil terdapat siswa sebanyak 44% terinfeksi STH, hal ini dikarenakan siswa yang kurang pengetahuan tentang kecacingan sebanyak 19 siswa (76%), sedangkan cukupnya pengetahuan tentang kecacingan sebanyak 4 siswa (16%), dan baiknya pengetahuan tentang kecacingan sebanyak 2 siswa (8%). dengan jumlah keseluruhan sebanyak 25 anak (100%). sebagian besar siswa maupun orang tua belum mengenal jenis-jenis cacing yang berbahaya bagi kesehatan, ataupun mengetahui cara pencegahan dan akibat kecacingan. Upaya yang dapat dilakukan adalah kegiatan penyuluhan yang berlangsu ng sangat interaktif dan komunikatif dan setiap peserta penyuluhan dapat memahami penjelasan yang disampaikan baik melalui media poster baik melalui tanya jawab. Berdasarkan hasil pretest dan post terdapat peningkatan pengetahun rata-rata 23% menjadi 71% ,sehingga total peningkatan pengetahuan peserta bertambah sebesar 48%.
ANALISA BAKTERI Escherichia coli DAN Salmonella sp PADA TELUR AYAM KAMPUNG Della Ika Putri Damayanti; Mutiarawati Diah Titik; Suliati Suliati
Analis Kesehatan Sains Vol. 10 No. 1 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.402 KB)

Abstract

Meanings of animal origin are often contaminate by microorganism, one of it is chicken eggs. Free range chicken is often consumed raw for medicinal purposes without knowing any contamination in it. Free range chicken are often associated with the genesis of foodborne diseases that can cause ailments in the form of diarrheal and gastroenteristic diseases. According to data provided by the 2018 ministry of health, there are approximately 7 million cases of diarrheal disease every year. The bacteria responsible for diarrhea is Salmonella sp, Escherichia coli and Staphylococcus aureus. This type of research is laboratory experimental research through culture. The population of this study was eggs obtained from the village of Tanggul Kulon RT 01/RW 07 Jember. The sample of this research is free range chicken taken by purposive sampling based on criteria. The place of this research was conducted at the Bacteriology Laboratory of Poltekkes Surabaya from January to April 2021. The result showed 4 out of 12 positive samples identified as Escherichia coli and 8 out of 12 positive samples identified as Salmonella parathypi A bacteria
UJI OPTIMALISASI DAYA HAMBAT EKSTRAK DAUN TEH HIJAU (Camellia Sinensis) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus Salsyabillah Ramadhani HD. Widya; Mutiarawati Diah Titik; Suliati Suliati
Analis Kesehatan Sains Vol. 10 No. 2 (2021): Analis Kesehatan Sains
Publisher : Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya

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Abstract

Research on substances as new antibiotics to inhibit or kill bacteria that are resistant to antibiotics needs to be done. One of the simplicia has an antibacterial effect is the leaves of green tea (Camellia sinensis). Antibacterial content the leaves of green tea (Camellia sinensis), namely alkaloids, saponins, tannins, and catechins (polyphenols). Tea one of the favorite products from Indonesia’ estate. The purpose of this study was to determine optimization of the inhibitory power the leaves of green tea’s (Camellia sinensis) extract against the growth of Escherichia coli and Staphylococcus aureus. This research is an experimental laboratory conducted at Bacteriology”s Laboratory, Department of Medical Laboratory Technology Surabaya in November 2020 - April 2021. This study uses the Kirby Bauer disk diffusion method, namely Mueller Hinton Agar (MHA) media which has been planted with Escherichia coli and Staphylococcus aureus with disks that have been prepared. soaked in the leaves of green tea’s (Camellia sinensis) extract at concentrations of 25%, 50%, 75%, and 100% and then incubated for 24 hours in an incubator. The results can be concluded the leaves of green tea’s (Camellia sinensis) extract can be used as an antibacterial. Optimal of results in this research were Escherichia coli at a concentration of 25% with an average diameter of 10,25 mm and Staphylococcus aureus at a concentration of 100% with an average of 22,5 mm. This is because content the leaves of green tea’s (Camellia sinensis) is catechins and alkaloids as strong antibacterials
Pemeriksaan Status Gizi dan Kadar Asam Urat Lansia di Posyandu Abiyoso Polkesbaya Evy Diah Woelansari; Juliana Christyaningsih; Suhariyadi Suhariyadi; Lully Hanni Endarini; Museyaroh Museyaroh; Diah Titik Mutiarawati; Wisnu Istanto; Suliati Suliati; Anik Handayati; Sri Sulami Endah Astuti; Pestariati Pestariati; Retno Sasongkowati; Mamik Mamik; Sri Utami; Minarti Minarti; Luthfi Rusyadi; Liliek Soetjiatie
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 2 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 2 Agustus 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i2.13417

Abstract

Proses penuaan dapat memengaruhi kesehatan fisik lansia dan meningkatkan risiko berbagai gangguan kesehatan, termasuk hipertensi dan asam urat. Kegiatan ini berlangsung pada tanggal 14 Juni 2023 bertempat di Balai RW 2 Kelurahan Kertajaya, Surabaya dan dihadiri 59 lansia yang berdomisili di RW 1 dan RW 2 Kelurahan Kertajaya Kecamatan Gubeng Surabaya. Jenis kegiatan yang dilaksanakan adalah pengukuran status gizi, asam urat dan senam hipertensi. Hasil pemeriksaan mengungkapkan 57,6% lansia memiliki kadar asam urat yang tinggi dan mayoritas memiliki kelebihan berat badan, sehingga lansia perlu mengendalikan tekanan darah dengan pengobatan yang diresepkan oleh dokter, perubahan gaya hidup seperti diet rendah garam, olahraga teratur, dan menghindari stres.
Pemeriksaan Kesehatan Warga Panti Yayasan Cinta Kasih Ibu Teresa, Sambikerep, Surabaya Juliana Christyaningsih; Kiaonarn Ongko Waluyo; Museyaroh Museyaroh; Nuning Marina Pengge; Lully Hani Endarini; Suliati Suliati; Nurul Hindaryani
Jurnal Pengabdian Kesehatan Beguai Jejama Vol 4, No 1 (2023): Jurnal Pengabdian Kesehatan Beguai Jejama Volume 4 Nomor 1 April 2023
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jpk.v4i1.8998

Abstract

Yayasan Cinta Kasih Ibu Teresa Sambikerep, Surabaya, Indonesia, menerima penitipan anak, khususnya anak terlantar dan lansia, berjumlah 48 orang terdiri dari 18 lansia, 20 anak, 10 remaja, sedangkan pengelola Yayasan Cinta Kasih sejumlah 6 orang. Sejak masa pandemi, anak dan lansia di Yayasan Cinta Kasih Ibu Teresa tersebut jarang mendapat layanan kesehatan dan keterbatasan ruanggerak anak-anak. Tim Pengabdi Polkesbaya mempunyai solusi yaitu anak di Yayasan Cinta Kasih Ibu Teresa akan mendapat pemeriksaan tumbuh kembang anak, Penyuluhan Hidup Bersih dan Sehat, pemeriksaan Status Gizi, penyuluhan cara menyikat gigi yang benar, sedang untuk dewasa dan lansia adalah pelayanan kesehatan berupa pemeriksaan tekanan darah, gula darah dan asam urat. Hasil yang didapat adalah Penyuluhan oleh tim pengabdi diikuti dengan baik dan antusias oleh warga Yayasan Cinta Kasih Ibu Teresa. Status gizi dan tumbuh kembang anak dan bayi di Yayasan Cinta Kasih Ibu Teresa didapatkan anak kategori kurus 3%, gemuk 9%, obesitas dan BB kurang masing2 3% dan BB lebih 6%, sedangkan 6% dewasa/ lansia didapatkan kadar glukosa darah dan asam urat tinggi. Disarankan kegiatan serupa dapat dilakukan secara periodik agar warga Yayasan Cinta Kasih Ibu Teresa dapat terpantau status kesehatannya.
Efektivitas Ekstrak Etanol Pegagan (Centella asiatica (L.) Urb) dalam Menghambat Pembentukan Biofilm Staphylococcus aureus Ilma Ilma; Diah Titik Mutiarawati; Suliati .
Jurnal Penelitian Kesehatan SUARA FORIKES 2022
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf13nk360

Abstract

Staphylococcus aureus has been the cause of a variety of community and nosocomial infections in humans, including folliculitis, impetigo, sepsis, endocarditis, osteomyelitis, implant-associated biofilms, and is also a major cause of recurrent infections in chronic wounds. This bacterium has a high efficiency in forming biofilms which results in continuous infection. One of the virulence factors of Staphylococcus is biofilm, which has the function of facilitating persistence in the host's body and weakening the host's defense system, resulting in resistant bacteria to high concentrations of antimicrobials. Centella asiatica (L.) Urb. or commonly known by the common name gotu kola, is a type of medicinal plant that has been used for a long time to treat and treat disease. Centella asiatica has various types of secondary metabolites that are able to effectively inhibit the formation of biofilms on bacteria. The purpose of this study was to determine the effectiveness of gotu kola ethanol extract as an inhibitor of Staphylococcus aureus biofilm formation. This type of research was a true experimental laboratory conducted in vitro with the microtiter dish assay method. The results obtained in this study were in the form of optical density values for biofilm density which were then processed using the SPSS application. The results showed that gotu kola extract was effective in inhibiting the formation of S. aureus ATCC 25923 biofilms as well as clinical isolates. The conclusion of this study is that the ethanol extract of Centella asiatica (L.) Urb) can inhibit the formation of Staphylococcus aureus biofilms with an MBIC value of 2 mg/mL.Keywords: Staphylococcus aureus; biofilms; gotu kola  ABSTRAK Staphylococcus aureus telah menjadi penyebab beragam infeksi pada manusia yang bersifat komunitas dan nosokomial, termasuk folikulitis, impetigo, sepsis, endokarditis, osteomielitis, biofilm terkait implant, dan juga merupakan salah satu penyebab utama infeksi yang berulang pada luka kronis. Bakteri ini memiliki efisiensi yang tinggi dalam membentuk biofilm yang mengakibatkan infeksi terjadi secara terus menerus. Salah satu faktor virulensi dari Staphylococcus adalah biofilm, yang memiliki fungsi memfasilitasi persistensi dalam tubuh host dan melemahkan sistem pertahanan host, sehingga mengakibatkan bakteri resisten pada antimikroba dengan konsentrasi tinggi. Centella asiatica (L.) Urb. atau yang biasa dikenal dengan nama umum pegagan, merupakan salah satu jenis tumbuhan obat yang telah digunakan sejak lama untuk mengobati dan mengatasi penyakit. Pegagan memiliki berbagai jenis zat metabolit sekunder yang mampu menghambat pembentukan biofilm pada bakteri secara efektif. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas ekstrak etanol pegagan sebagai penghambat pembentukan biofilm bakteri Staphylococcus aureus. Jenis penelitian ini adalah true experimental laboratoris yang dilakukan secara in vitro dengan metode microtiter dish assay. Hasil yang didapatkan pada penelitian berupa nilai optical density kepadatan biofilm yang kemudian diolah menggunakan aplikasi SPSS. Hasil penelitian menunjukkan bahwa ekstrak pegagan efektif dalam menghambat pembentukan biofilm S. aureus ATCC 25923 maupun isolat klinis. Kesimpulan dari penelitian ini adalah ekstrak etanol pegagan (Centella asiatica (L.) Urb) dapat menghambat pembentukan biofilm Staphylococcus aureus dengan nilai MBIC 2 mg/mL.Kata kunci: Staphylococcus aureus; biofilm; pegagan
Co-Authors ., Pestariati Anita Dwi Anggraini Anita Dwi Anggraini Ayu Puspitasari Della Ika Putri Damayanti Diah Titik Mutiarawati Dwi Agustin Wulandari Endah Prayekti Endarini, Lully Hanni Evy Diah Woelansari Handayati, Anik Ilma Ilma Juliana Christyaningsih Kiaonarn Ongko Waluyo Liliek Soetjiatie Lully Hani Endarini Luthfi Rusyadi Mamik Mamik Minarti Minarti Museyaroh, Museyaroh Mutiarawati, Diah Titik Nuning Marina Pengge Nurul Hindaryani Retno Sasongkowati Retno Sasongkowati Salsyabillah Ramadhani HD. Widya Sri Sulami Endah Astuti Sri Utami Suhariyadi Suhariyadi Widyanur Wisnu Istanto Wisnu Istanto