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Parluhutan Siahaan
Jurusan Biologi Fakultas MIPA Universitas Sam Ratulangi Manado 95115
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Pendampingan Masyarakat Petani di Tomohon Dalam Pemanfaatan Biopestisida dari Jamur Beauveria bassiana Isolat Lokal Untuk Pengendalian Hama-Hama Tanaman Pangan Siahaan, Parluhutan; Siahaan, Saroyo
The Studies of Social Sciences Vol. 6 No. 2 (2024): The Studies of Social Sciences
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35801/tsss.v6i2.60307

Abstract

Untuk mengatasi permasalahan yang sedang dihadapi petani maka perlu dilakukan solusi untuk mengatsinya. Solusi yang akan dilakukan adalah dengan penerapan teknologi pengendalian hayati yang menggunakan jamur B. bassiana yang disekitar lahan pertanian mereka. Untuk itu maka akan dilakukan hal-hal sebagai berikut: a). Edukasi Masyarakat Petani Mitra tentang pengenalan organisme merugikan dan organisme menguntungkan yang ada di lahan persawahan petani mitra, edukasi tentang pengetahuan dampak-dampak negatif penggunaan pestisida sintetik dan cara pemakaian pestisida dengan cara yang dan dosis yang tepat serta seminimum mungkin, edukasi tentang cara bertani yang sehat dan berwawasan lingkungan. b). Penerapan Teknologi Pengendalian Hayati Beauveria bassiana isolat lokal dalam bentuk pelatihan atau workshop. Hal yang akan dilatih dalam penerapan ini teknologi yaitu: Teknik eksplorasi jamur entomopatogen dari lahan pertanian petani mitra sendiri, Teknik isolasi B. bassiana, Teknik perbanyakan jamur entomopatogen B. bassiana dengan media sederhana, Teknik Formulasi jamur B. bassiana agar siap pakai, Teknik Aplikasi B. bassiana di lapangan dengan menggunakan jamur B. bassiana yang telah dieksplorasi dan diisolasi, Teknik penyimpanan isolat B. bassiana. c). Penyediaan alat yang dibutuhkan. Hasil kegiatan Petani mitra mengenal dan dapat membedakan jenis-jenis organisme yang merusak dan mana yang menguntungkan, petani mitra mengenal teknik pengendalian hama yang lebih ramah lingkungan, petani mitra siap untuk mengurangi pembelian pestisida sintetik, petani mitra siap untuk melakukan cara bertani yang berwawasan lingkungan dan petani mampu membuat biopestisida dari jamur B. bassiana dan menerapkannya pada lahan pertanian mereka.
Morphological Characteristics and Antagonist Test Trichoderma sp. Against Fungi Helminthosporium sp. Causing Leaf Blight Disease in Corn Plants In Vitro Santoso, Ardelia Qonita; Siahaan, Parluhutan; Baideng, Eva
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.60620

Abstract

Helminthosporium sp. is an organism that causes leaf blight disease in corn plants, thus becoming one of the causes of declining production in the agricultural sector. Lack of awareness and low knowledge of farmers in control efforts that still use synthetic pesticides that have a negative impact on the environment and health, therefore the use of antagonistic microbes that live around plant roots such as Trichoderma sp. is one way to control environmentally friendly soil-borne pathogens. This study aims to analyze the characteristics of Trichoderma sp. and Helminthosporium sp. macroscopically and microscopically and to test the antagonistic ability of Trichoderma sp. fungi based on competition tests, antibiosis tests and mycoparasitism tests against Helminthosporium sp. fungi isolate Tondegesan. Trichoderma sp. fungal antagonist test against Helminthosporium sp. fungi using the duo plate assay method in Potato Dextrose Agar (PDA) media in vitro. The results showed that Trichoderma sp. fungi. has macroscopic morphological characteristics of dark green colonies with fine cotton fibers and microscopic morphological characteristics of green septate hyphae, upright conidiophores and many branches. Helmintosporium sp. fungi have macroscopic morphological characteristics of flat fibrous colony surfaces, oval shapes with slightly brownish white colors and microscopic morphological characteristics of fairly thick hyphal walls with yellowish brown colors. Based on the antagonist test, Trichoderma sp. fungi have an inhibition percentage of 71% against Helminthosporium sp. fungi on the seventh day.
Antagonist Test of Gliocladium sp. against Fusarium sp. Cause of Wilt Disease in Shallot Plants In Vitro Sarayar, Dea; Umboh, Stella Deiby; Siahaan, Parluhutan
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62836

Abstract

This study aims to analyze the morphological characteristics of the fungus Gliocladium sp., measure its inhibition against the growth of the fungus Fusarium sp. causing wilt disease in shallot plants (Allium ascalonicum L), and examine its antagonistic mechanism in vitro. This research method includes exploration and sampling, sterilization of tools and materials, making PDA media, isolation, purification, and identification of morphological characteristics and measurement of antagonistic fungal inhibition. The antagonist test uses the dual culture method, and data analysis with a completely randomized design (CRD) and ANOVA test followed by the Honestly Significant Difference (HSD) Test at the 5% significance level. The results showed that Gliocladium sp. was able to inhibit the growth of Fusarium sp. with a percentage inhibition of 25.63% and spore viability of 66.52%. Antagonistic mechanisms observed include competition for space and nutrients, antibiosis, lysis, and mycoparasitism.
Co-Authors Adelfia Papu Ai S Nio Anwar Sahid Baideng, Eva Desti, Yunita DINGSE PANDIANGAN Edwin de Queljoe Kakanga, Christin J.R. Koda, Marlince Mangais, Rowland Mangansige, Crestie T. Marhaenus J. Rumondor Marnix L.D. Langoy Marnix Langoy Mereh, Risa Junita Nio Song Ai Orpa Smarce Fransina Manori Patricia Claudya Torey Pirade, Monalisa Rampe, Henny L. Redsway T.D Maramis Roni Koneri Sagay, Kezia Sisilia Saimima, Arie J. Santoso, Ardelia Qonita Sarayar, Dea Saroyo Saroyo Siahaan, Saroyo Sonke, Natasya Gloria Susan M Mambu Susan Mambu Susan Marlein Mambu Tangapo, Agustina Monalisa Tjolleng, Fitria Tri A. Mokodompit Umboh, Stella D. Wongkar, Jusak Wowiling, Susan Yelni Gogahu, Yelni Yunia E Banyo