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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Veteriner Jurnal Kedokteran Hewan
Sri Gustari
Departemen Reproduksi Dan Kebidanan Fakultas Kedokteran Hewan Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

 1 
Fetal Bovine Serum Meningkatkan Maturasi Inti Oosit Kelinci Setelah Dimaturasi Secara In Vitro Ni Wayan Kurniani Karja; Winny Plumeria Aqshani; Yesi Pratiwi Kusumawati; Veronika Gilang Pravitasari; Sri Gustari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was conducted to examine the meiotic competence or nuclear maturation of rabbit oocytesmatured in vitro. Oocytes were recovered by mincing the ovaries in modified phosphate buffer saline (m-PBS). Selected cumulus-oocyte complexes (COCs) with compact cumulus cell mass and a dark, homogenousooplasm were cultured in maturation medium at 38°C in a humidified atmosphere of 5% CO2 in air, andthen stained for nuclear maturation. Three experiments were carried out. In Experiment 1, COCs werecultured in maturation medium for 18-20, 22-24, and 28-30 h. The proportion of oocytes at metaphase II(MII) was similar (P>0.05) at 18-20 (69.2%), 22-24 (66.4%), and 28-30 (71.1) h of culture. In Experiment 2,COCs were cultured in either maturation medium containing 0.04% bovine serum albumine (BSA) or 5%fetal bovine serum (FBS) for 24 h. The proprotion of oocytes that reached MII were higher (P<0.05) in FBSgroup (79.2%) than those of oocytes in BSA group (64%). In Experiment 3, based on the presence or absenceof follicles and corpora lutea, the ovarian pairs were classified into follicular or luteal stages. There was nodifference (P>0.05) among oocytes collected from ovaries in follicular (79.7%) and luteal stages (78.7%) inthe ability to achieve nuclear maturation. These results indicated that nuclear maturation of rabbitoocytes in vitro was completed at 18-20 h of maturation culture and was not affected by ovary’s reproductivestage. The presence of FBS in the maturation medium enhanced the ability of rabbit oocytes to achievenuclear maturation.
Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin Sri Gustari; Ni Wayan Kurniani Karja; Yuke Rizky Amelia; Ian Kurniawan; Bayu Sulistyo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytesafter in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediatelyafter slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in aflask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocyteswere observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitromaturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Followingthis, oocytes were observed under inverted microscope for first polar body extrusion, then stained withaceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinalvesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphaseII, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytesmaturation using media supplemented with either BSA or NCS.
Co-Authors Agung Budiyanto Agung Budiyanto Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bayu Sulistyo Delvi Ramadayani Dito Anggoro Donata Asta Dwi Jatmoko Eka Wahyu Nugraha Erif Maha Nugraha Setyawan Erif Maha Nugraha Setyawan Ian Kurniawan Jayanti Berliana Dewi Julian A.Bartolome Julian A.Bartolome Julian A.Bartolome Lalu Unsunnidhal Marchellia Dhiafifah Wahyunandha Melati Kusuma Bunda Pertiwi Mujahidatul Musfiroh Ni Wayan Kurniani Karja Prabowo P Putro Silvana Nugraheni Slamet Subagyo Surya Agus Prihatno Topas Wicaksono Priyo Jr Veronika Gilang Pravitasari Winny Plumeria Aqshani Yesi Pratiwi Kusumawati Yosua Kristian Adi Yuke Rizky Amelia