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All Journal Jurnal Pangan dan Agroindustri Indonesian Journal of Human Nutrition Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Jurnal Abdimas Mahakam JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Caring : Jurnal Pengabdian Masyarakat
Rahma Micho Widyanto
Departemen Gizi, Fakultas Ilmu Kesehatan, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Electrical & Electronics Engineering Health Professions Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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In Vitro Evaluation of Cytotoxicity Effect of Ihau Fruit Extract (Dimocarpus longan var. Malesianus Leenh.) on MCF-7 Breast Cancer Cell Line Irma Sarita Rahmawati; Ghina Putri Dyanti; Rahma Micho Widyanto; Annisa Rizky Maulidiana; Wyna Nabila; Ratna Chrismiari Purwestri
Indonesian Journal of Human Nutrition Vol. 10 No. 1 (2023)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2023.010.01.7

Abstract

Cancer prevalence is steadily increasing each year and becomes the second leading cause of death worldwide. In Indonesia, breast cancer had a prevalence of 16.7% in 2018. Free radicals contribute to the occurrence of breast cancer, while antioxidants play a vital role in protecting cells and repairing the damage caused by free radicals. Ihau, an endemic fruit in Kalimantan Island, contains phytochemical compounds with potential antioxidant and anticancer properties. Utilizing local food as natural antioxidants could serve as an alternative for breast cancer prevention and treatment. This study aims to assess the anticancer potential of Ihau fruit extract on the MCF-7 breast cancer cell line. A post-test-only control group design method using the MTT assay was used, with two treatment groups: water and 96% ethanol extract, and four different concentrations (125, 250, 500, and 1000 ppm), and each was replicated three times. Statistical analysis using the ANOVA test found no significant difference among all concentrations. The IC50 values of the cytotoxic activity of water and ethanol extracts were 1,197.7 ppm and 1,148 ppm, respectively. It can be concluded that both water and ethanol extract of Ihau fruit exhibited very weak cytotoxic activity.
Uji Deteksi Adulterasi Daging Babi (Sus scrofa domestica) pada Bakso Metode Loop-Mediated Isothermal Amplification Rahma Micho Widyanto; Cleonara Yanuar Dini; Irma Sarita Rahmawati; Sekar Ramadhanti Putri; Amelia Nurdini Rozana; Sakinah Hilya Abida; Yunimar Yunimar
Indonesian Journal of Human Nutrition Vol. 8 No. 1 (2021)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2021.008.01.8

Abstract

Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to identify the presence of pork DNA in meatballs. Six primers were used for the identification of mitochondrial DNA cytochrome oxidase subunit II. Samples were prepared in the form of mixed beef meatballs containing pork with a concentration of 50; 5; 0.5; 0.05%, respectively. The results showed that the optimal concentration for primers was 4 pmol inner primers, 2 pmol loop primers, and 1 pmol outer primers. The LAMP reaction showed a change in color in the meatball sample with the pork mixture to the lowest concentration. The LAMP technique provides a faster test with the final result of on-the-spot color visualization so that it is potential to be developed as a quick detection kit for identification of halal food processed products
Kandungan Gizi dan Uji Organoleptik Beras Analog Kedelai Edamame dan Rumput Laut Eunike Cecilia Setiawan; Diyah Ayu Puspitasari; Shinta Kirana Rakhmani; Muhammad Naufal Rizqi Alfani; Arvin Wahyu Nor Imam; Rahma Micho Widyanto
Indonesian Journal of Human Nutrition Vol. 9 No. 1 (2022)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2022.009.01.1

Abstract

Diabetes Melitus Tipe 2 (DMT2) merupakan penyakit dengan karakteristik hiperglikemia yang disebabkan oleh resistensi insulin, gangguan sekresi insulin, maupun keduannya. DMT2 dapat diperbaiki melalui penatalaksanaan secara non farmakologi salah satunya melalui terapi nutrisi medis. Beras analog menjadi solusi alternatif bahan pangan sumber karbohidrat yang lebih sehat sebagai pengganti beras putih bagi pasien DMT2. Penelitian ini bertujuan untuk membuat produk beras analog kombinasi tepung kedelai edamame dan rumput laut untuk mengetahui hasil organoleptik dan kandungan gizinya. Uji organoleptik dilakukan kepada 25 panelis terlatih untuk mengetahui tingkat kesukaan berdasarkan rasa, aroma, warna, dan tekstur. Untuk mengetahui kandungan gizi, dilakukan uji proksimat dan uji anthrone. Penelitian ini merupakan penelitian eksperimental dengan 5 perlakuan dan 3 ulangan. Data hasil analisis diuji secara statistik menggunakan uji ANOVA dan dilanjutkan dengan uji Post Hoc Tukey. Persentase tingkat kesukaan tertinggi secara keseluruhan berada pada beras analog F3 (50%TE+50%TRL) yang mengandung karbohidrat sebesar 83,71 g, protein 2,76 g, lemak 0,23 g, kadar air 12,89 ml, abu 0,41 g, gula 0,18 g, serat larut 3,89 g, serat tidak larut 3,44 g total serat 7,29 g dan energi sebesar 346,92 kkal dalam 100 g. Uji statistika menunjukkan terdapat perbedaan yang signifikan antara kandungan gizi beras analog dengan beras putih (p<0,05).  
Co-Authors Adelia Paradya Zetta Adelya Desi Kurniawati Akhiyan Hadi Susanto Amelia Nurdini Rozana Annisa Rizky Maulidiana Ardhila Lovi Hasinofa Arvin Wahyu Nor Imam Budi Utomo Budi Utomo Choirun Nisa Riski Dian Handayani Dini, Cleonara Yanuar Diyah Ayu Puspitasari Eka Nanda Wulandar Eunike Cecilia Setiawan Frisa Inda Vega Br Silalahi Fuadiyah Nila Kurniasari Ghina Putri Dyanti Ilzamha Hadijah Rusdan Ilzamha Hadijh Rusda Inggita Kusumastuty Irma Sarita Rahmawati Johanna Angkasa Putri Laksmi Karunia Tanuwijaya Muhammad Naufal Rizqi Alfani Muhammad Surya Madani Nia Fitriani Aisyah Nisa Aisyah Nur Amaliah Ahmad Nurona Azizah Rahmawati, Siti Irma Rakhmawati Widya Safitri Ratna Chrismiari Purwestri Reyna Rachma Wati Rifanty Meydiana Rachmawati Putri Rustiana Tasya Ariningpraja Sakinah Hilya Abida Sekar Ramadhanti Putri Shinta Kirana Rakhmani Sri Andarini Tifany Prasaja Titis Sari Kusuma Wahyu Alfyati Regina Wahyu Diah Proborini Wahyu Diah Proborini Wisnasari, Shila Wyna Nabila Yosfi Rahmi Yunimar Yunimar YUNIMAR YUNIMAR Zhafirah Salma Putri