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PENGARUH PENGGUNAAN SILASE RUMPUT GAJAH YANG DIBERI SINGKONG TERHADAP KONSENTRASI VFA DAN N-NH3 CAIRAN RUMEN SAPI SECARA IN VITRO Fitriyanto, Rifqi; Suhartati, Fransisca Maria; Rahayu, Sri
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background. This study was aimed to examine the effect of using elephant grass silage fed with cassava on the concentration of VFA N-NH3 which was carried out from 7 June to 7 July at the Laboratory of Animal Nutrition and Food Science, Faculty of Animal Husbandry, Universitas Jenderal Soedirman Purwokerto. Materials and methods The materials used in this study were rumen fluid from three beef cattles taken from the slaughterhouse of Bantarwuni Village, Kembaran District, Banyumas Regency immediately after the cattle were slaughtered, silage of elephant grass, cassava, and a set of in vitro tools. The study was carried out using in vitro methods and using a randomized design (CRD) with 4 complete treatments. Each treatment was repeated 5 times. The treatments tested were R1 = 100% DM of elephant grass silage; R2 = 95% DM of elephant grass silage + 5% DM of cassava; R3= 90% DM of elephant grass silage + 10% DM of cassava; R4 = 85% DM of elephant grass silage + 15% DM of cassava. The variables measured were the concentration of VFA and N-NH3. The data obtained were analyzed for variance and if the treatment had an effect on the measured variables, it was continued with the Honestly Significant Difference (HSD) test. Results. The average concentration of VFA is R1 : 122 mM, R2 : 126 mM, R3 : 128 mM, R4 : 130 mM, while the average concentration of N-NH3 is R1 : 2.4 mM, R2 : 3.4 mM, R3 : 4 mM, R4 : 3 mM. The results of the analysis of variance shows that the use of elephant grass silage fed with cassava had no significant effect (P>0.05) on the concentration of VFA but has a very significant effect (P<0.01) on the concentration of N-NH3. The highest VFA concentration is achieved by rumen fluid which obtained 85% silage of elephant grass and 15% cassava, although statistically there is no difference. Conclusion. The average concentration of N-NH3 is still low, but the highest concentration is achieved by rumen fluid added with 90% elephant grass silage and 10% cassava.

PRODUK FERMENTASI RUMEN SAPI POTONG SECARA IN VITRO YANG DIBERI PAKAN SILASE DAUN NANAS SEBAGAI PENGGANTI RUMPUT GAJAH Saputro, Alif Rizki Tixko; Suhartati, Fransisca Maria; Rimbawanto, Efka Aris
ANGON: Journal of Animal Science and Technology Vol 4 No 1 (2022): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Background. This study aims to examine the effect of using pineapple leaf waste silage as a substitute for elephant grass based on levels of Nitrogen-ammonia (N-NH3) and Volatile Fatty Acid (VFA) concentration in total rumen fluid of beef cattle in vitro. The research was conducted from August 25 to September 20, 2022 at the Laboratory of Nutrition and Animal Feed Science, Faculty of Animal Science, Jenderal Sudirman University, Purwokerto. Materials and methods. The study using an in vitro method with a completely randomized design, 5 treatments and each treatment was repeated 4 times so that there were 20 research units. The treatments used consisted of P0 (60% concentrate + 40% elephant grass), P1 (60% concentrate + 30% elephant grass + 10% pineapple leaf silage), P2 (60% concentrate + 20% elephant grass + 20% pineapple leaf silage), P3 (60% concentrate + 10% elephant grass + 30% pineapple leaf silage), and P4 (60% concentrate and 40% pineapple leaf silage). Results. The results showed that the treatment had no significant effect (P>0.05) on the levels of N-NH3 and the concentration of VFA. Conclusion. It was concluded that the use of pineapple leaf silage at a balance of 40% elephant grass and 60% concentrate could replace elephant grass in beef cattle feed up to 100% based on in vitro measurements of N-NH3 levels and total VFA concentrations.

konsumsi KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN SAPI BALI JANTAN YANG DIBERI COMPLETE RUMEN MODIFIER Syafiq, Muhammad; Suhartati, Fransisca Maria; Yuwono, Pambudi
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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ABSTRACT Research aimed to examine the effect of giving Complete Rumen Modifier on the intake and digestibility of Crude Protein (CP) feed of Bali bulls. The material used is 3 male Balinese cows, the feed given consists of concentrate 2% of the cow's body weight and voluntary feed intake hay. The concentrate is composed of onggok 38,58%, rice bran 14,29%, coconut cake 13,14%, palm cake 10%, pollard 9,43%, molasses 4,86%, coffee husk 4,8%, dolomite 1,43%, aminosine 1,2%, salt 0,86%, urea 0,86%, zeolite 0,57%, and minerals 0,28%. The composition of CRM consists of noni leaf flour 30%, cassava vine leaf meal 30%, dry tea pulp 30%, Saccharomyches cerevisiae 3%, methionin 2%, urea 1% and sulfur 4%. The research used the experimental method of Latin Square Design, there were three treatments tested, namely P0 (concentrate 2% body weight cow and straw voluntary feed intake), P1 (P0 + CRM 0.5% from Dry Matter concentrate), and P2 (P0 + CRM 1% from concentrate). The variables measured are intake and Digestibility of crude protein, the data obtained were tested using analysis of variance and differences in treatment with the Orthogonal Polynomial test. The results of the analysis of variance showed that the treated feed given CRM had no significant effect (P>0.05) on PK digestibility. Meanwhile, PK consumption had a significant effect (P<0.05) on feed treated with P1 and P2 compared to P0. The conclusion obtained from this study is that the provision of CRM can increase consumption of PK while the digestibility of PK decreases when given CRM.

the effect PENGARUH PENGGANTIAN KONSENTRAT MENGGUNAKAN AMPAS TEMPE DAN SUPLEMENTASI COMPLETE RUMEN MODIFIER (CRM) TERHADAP KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN DOMBA Hutasoit, Irma Uli; Suhartati, Fransisca Maria; Suryapratama, Wardhana
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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The study aims to examine the effect of concentrate replacement using tempeh dregs and Complete Rumen Modifier (CRM) supplementation on the consumption and digestibility of crude protein in sheep feed. The study used 18 local male sheep aged 7-8 months with an average weight of 20±2.34 kg. This study used a Randomized Group (RAK) design and used a type of experimental research, as a group as well as a repeat of the initial body weight of sheep after the adaptation period as many as 6 groups and 3 treatments, including P1: Ammoniated rice straw ad libitum + concentrate 2.5% DM of body weight (control), P2: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight, and P3: Ammoniated rice straw ad libitum + tempeh dregs 2.5% DM of body weight + 1% CRM. The variables observed in the study were consumption and digestibility of crude protein. Based on the results of the analysis of treatment variance, the effect was very real (P<0.01) on crude protein consumption with an average consumption of P1, P2, P3 treatments, respectively 10.26±0.05; 14.35±0.78; 14.02±0.88 (g/head/d) and a significant effect (P<0.005) on digestibility with crude protein digestibility rate of P1, P2, P3 treatment respectively at 59.44±3.65%; 67.85±3.44%; 63.56±4.83%. Based on orthogonal contrast tests, tempeh dregs can replace concentrate feed entirely and even increase crude protein consumption and digestibility by 3.90% and 3.93% respectively without 1% CRM supplementation. Keywords: CRM, tempe dregs, lamb, crude protein, consumption, digestibility.

KECERNAAN BAHAN KERING DAN BAHAN ORGANIK PAKAN BERBASIS INDEKS SINKRONISASI PROTEIN DAN ENERGI SECARA IN-VITRO Kalpikorini, Diah Ayu; Suhartati, Fransisca Maria; Syamsi, Afduha Nurus
ANGON: Journal of Animal Science and Technology Vol 6 No 2 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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Pakan berbasis indeks sinkronisasi protein-energi (SPE) merupakan pakan yang disusun dengan memperhatikan kebutuhan mikroba rumen khususnya pada suplai energi dan N-protein, sehingga kinerja mikroba dalam rumen dapat meningkat dalam proses fermentasi pakan. Tujuan dilakukan penelitian ialah untuk mengkaji pengaruh indeks Sinkronisasi Protein Energi akan terhadap kecernaan bahan kering dan kecernaan bahan organik secara in-vitro. Penelitian dilaksanakan menggunakan metode ekperimental secara In-vitro. Materi penelitian yang digunakan adalah cairan rumen 3 ekor Kambing Jawarandu yang diambil dari rumah potong hewan (RPH) Sokaraja, Purwokerto, segera setelah kambing dipotong. Bahan pakan yang digunakan terdiri dari rumput odot, indigofera, bungkil kelapa, onggok, dedak, ampas tahu, dan mineral mix. Perlakuan yang digunakan adalah pakan dengan indeks SPE P1 (0,55), P2 (0,60), P3 (0,65), P4 (0,70), P5 (0,75). Penelitian dilaksanakan secara eksperimental dengan Rancangan Acak Lengkap (RAL). Variabel yang diukur yaitu Kecernaan Bahan Kering (KcBK) dan Kecernaan Bahan Organik (KcBO). Penelitian diuji dengan analisis variansi dan orthogonal polinomial (OP). Nilai KcBK yang didapatkan secara berurut yaitu, P1: 64,76% ± 1,95; P2: 68,17% ± 1,49; P3: 71,49% ± 1,09; P4: 69,99% ± 1,08; dan P5: 69,69% ± 2,65. Nilai KcBO secara berurut yaitu, P1: 68,62% ± 3,65; P2: 74,48% ± 0,79; P3: 80,38% ± 2,02; P4: 78,49% ± 3,39; P5: 76,16% ± 5,47. Berdasarkan hasil analisis variansi, indeks SPE berpengaruh sangat nyata (P<0,01) terhadap kecernaan bahan kering (KcBK) dan kecernaan bahan organik (KcBO) secara in-vitro. Berdasarkan uji lanjut OP indeks SPE pakan berpengaruh secara kuadratik terhadap KcBK dengan persamaan Y=-92,338 + 477,94X - 349,71X2, R2:0,58, perhitungan titik puncak P (0,683;70,95%) artinya indeks SPE yang optimum sebesar 0,683 akan menghasilkan kecernaan bahan kering sebesar 70,95%. Hasil yang sama juga pada KcBO dengan persamaan Y= 237,54 + 936,06X -690,7X2, R2:0,55, perhitungan titik puncak P (0,667;79,6%) artinya indeks SPE yang optimum sebesar 0,667 akan menghasilkan kecernaan bahan organik sebesar 79,6%. Kecernaan bahan kering dan kecernaan bahan organik secara in-vitro yang terbaik terdapat pada pakan dengan indeks SPE 0,68.

PENGARUH PENGGUNAAN COMPLETE RUMEN MODIFIER DALAM PAKAN YANG DISUSUN BERDASARKAN INDEKS SINKRONISASI PROTEIN ENERGI TERHADAP KONSUMSI DAN KECERNAAN BAHAN KERING PAKAN DOMBA Khasan, Khasan; Suhartati, Fransisca Maria; Haryoko, Imbang
ANGON: Journal of Animal Science and Technology Vol 6 No 2 (2024): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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Penelitian bertujuan untuk mengkaji pengaruh interaksi antara penggunaan Complete Rumen Modifier(CRM) dalam pakan yang disusun berdasarkan Indeks Sinkronisasi Protein Energi (ISPE) terhadap konsumsi dan kecernaan bahan kering pakan domba. Penelitian ini sudah dilaksanakan selama 4 bulan yang dimulai dari bulan Mei sampai bulan September 2023. Metode penelitian yang digunakan yaitu eksperimental menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 2x2 dengan menggunakan bobot badan domba sebagai kelompok dan pengulangan sebanyak 5 kali. Perlakuan yang diberikan ada 4, yaitu P1 = Pakan tanpa penggunaan CRM dengan Indeks Sinkronisasi Protein Energi (SPE) 0,6, P2 = Pakan dengan penggunaan CRM 1% dari BK pakan dan Indeks Sinkronisasi Protein Energi (ISPE) 0,6, P3 = Pakan tanpa penggunaan CRM dengan Indeks Sinkronisasi Protein Energi (ISPE) 0,7, dan P4 = Pakan dengan penggunaan 1% CRM dari BK pakan dengan Indeks Sinkronisasi Protein Energi (ISPE) 0,7. Variabel yang diukur dalam penelitian ini adalah konsumsi dan kecernaan bahan kering pakan domba. Data yang diperoleh dianalisis dengan ANAVA kemudian diuji lanjut menggunakan uji Duncan's Multiple Range Test (DMRT). Berdasarkan hasil perhitungan dapat disimpulkan bahwa pakan yang menggunakan CRM 1% dan disusun berdasarkan indeks SPE 0,7 merupakan pakan terbaik ditinjau berdasarkan konsumsi dan kecernaan bahan kering pakan domba.

PENGARUH PENGGANTIAN KONSENTRAT MENGGUNAKAN AMPAS TEMPE DAN SUPLEMENTASI COMPLETE RUMEN MODIFIER (CRM) TERHADAP KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN DOMBA Hutasoit, Irma Uli; Suhartati, Fransisca Maria; Suryapratama, Wardhana
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

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KONSUMSI DAN KECERNAAN PROTEIN KASAR PAKAN SAPI BALI JANTAN YANG DIBERI COMPLETE RUMEN MODIFIER Syafiq, Muhammad; Suhartati, Fransisca Maria; Yuwono, Pambudi
ANGON: Journal of Animal Science and Technology Vol 6 No 3 (2024): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACTResearch aimed to examine the effect of giving Complete Rumen Modifier on the intake and digestibility of Crude Protein (CP) feed of Bali bulls. The material used is 3 male Balinese cows, the feed given consists of concentrate 2% of the cow's body weight and voluntary feed intake hay. The concentrate is composed of onggok 38,58%, rice bran 14,29%, coconut cake 13,14%, palm cake 10%, pollard 9,43%, molasses 4,86%, coffee husk 4,8%, dolomite 1,43%, aminosine 1,2%, salt 0,86%, urea 0,86%, zeolite 0,57%, and minerals 0,28%. The composition of CRM consists of noni leaf flour 30%, cassava vine leaf meal 30%, dry tea pulp 30%, Saccharomyches cerevisiae 3%, methionin 2%, urea 1% and sulfur 4%. The research used the experimental method of Latin Square Design, there were three treatments tested, namely P0 (concentrate 2% body weight cow and straw voluntary feed intake), P1 (P0 + CRM 0.5% from Dry Matter concentrate), and P2 (P0 + CRM 1% from concentrate). The variables measured are intake and Digestibility of crude protein, the data obtained were tested using analysis of variance and differences in treatment with the Orthogonal Polynomial test. The results of the analysis of variance showed that the treated feed given CRM had no significant effect (P>0.05) on PK digestibility. Meanwhile, PK consumption had a significant effect (P<0.05) on feed treated with P1 and P2 compared to P0. The conclusion obtained from this study is that the provision of CRM can increase consumption of PK while the digestibility of PK decreases when given CRM.

Kecernaan Protein Kasar dan Serat Kasar Pakan Kambing yang Diberi Daun Sirih (Piper betle Linn) dalam Pakan Berbasis Jerami Padi Amoniasi Ahmad, Miftahuddin; Suhartati, Fransisca Maria; Widyastuti, Titin
ANGON: Journal of Animal Science and Technology Vol 2 No 2 (2020): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2020.2.2.p106-116

PENGARUH PENGGUNAAN SILASE RUMPUT GAJAH YANG DIBERI SINGKONG TERHADAP KONSENTRASI VFA DAN N-NH3 CAIRAN RUMEN SAPI SECARA IN VITRO Fitriyanto, Rifqi; Suhartati, Fransisca Maria; Rahayu, Sri
ANGON: Journal of Animal Science and Technology Vol 3 No 3 (2021): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2021.3.3.p272-279

Background. This study was aimed to examine the effect of using elephant grass silage fed with cassava on the concentration of VFA N-NH3 which was carried out from 7 June to 7 July at the Laboratory of Animal Nutrition and Food Science, Faculty of Animal Husbandry, Universitas Jenderal Soedirman Purwokerto. Materials and methods The materials used in this study were rumen fluid from three beef cattles taken from the slaughterhouse of Bantarwuni Village, Kembaran District, Banyumas Regency immediately after the cattle were slaughtered, silage of elephant grass, cassava, and a set of in vitro tools. The study was carried out using in vitro methods and using a randomized design (CRD) with 4 complete treatments. Each treatment was repeated 5 times. The treatments tested were R1 = 100% DM of elephant grass silage; R2 = 95% DM of elephant grass silage + 5% DM of cassava; R3= 90% DM of elephant grass silage + 10% DM of cassava; R4 = 85% DM of elephant grass silage + 15% DM of cassava. The variables measured were the concentration of VFA and N-NH3. The data obtained were analyzed for variance and if the treatment had an effect on the measured variables, it was continued with the Honestly Significant Difference (HSD) test. Results. The average concentration of VFA is R1 : 122 mM, R2 : 126 mM, R3 : 128 mM, R4 : 130 mM, while the average concentration of N-NH3 is R1 : 2.4 mM, R2 : 3.4 mM, R3 : 4 mM, R4 : 3 mM. The results of the analysis of variance shows that the use of elephant grass silage fed with cassava had no significant effect (P>0.05) on the concentration of VFA but has a very significant effect (P<0.01) on the concentration of N-NH3. The highest VFA concentration is achieved by rumen fluid which obtained 85% silage of elephant grass and 15% cassava, although statistically there is no difference. Conclusion. The average concentration of N-NH3 is still low, but the highest concentration is achieved by rumen fluid added with 90% elephant grass silage and 10% cassava.

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