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All Journal Majalah Kedokteran Bandung Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Universa Medicina Jurnal Kesehatan Manarang Jurnal Analis Kesehatan Jurnal Kesehatan Borneo Journal of Medical Laboratory Technology JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Prosiding Seminar Kesehatan Masyarakat Prosiding Seminar Nasional Unimus Lontara Journal Of Health Science And Technology
Andri Sukeksi
Analis Kesehatan Fakultas Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Journal : Jurnal Analis Kesehatan

 1 
Pengaruh Sentrifugasi Spesimen Darah Anemia Terhadap Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Slide Handayani, Sri Puji; Sukeksi, Andri; Afriansyah, Muhammad Ardi
Jurnal Analis Kesehatan Vol. 11 No. 1 (2022): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v11i1.3196

Abstract

Anemic patients often have low hemoglobin and red blood cell level, which on the slide method and ABO system blood type test is used to obtain low degree of agglutination. Addition of treatment of a centrifugation was used to obtain a stronger degree of agglutination. The purpose of this research was to determine the difference degree of agglutination between centrifuged and uncentrifuged blood specimens in anemic patients. Quasi experimental research with non-equivalent control group design was conducted in March 2022 at the Hematology Laboratory, Universitas Muhammadiyah Semarang. The research sample were 16 specimens of anemic patients for slide method and ABO system blood type test. All samples were checked for blood type testbefore being centrifuged, then re-checked after the centrifugation there only concentrated red blood cell deposit were taken with only a small amount of plasma. The data from the test were analyzed using the Kruskal Wallis method. Comparison anti-A, anti-B, and anti-AB between centrifuged and uncentrifuged blood specimens there was a significant difference (p value < 0,005 means p = 0,002). The result showed that there were effect by centrifugation for the degree of agglutination improved better in centrifuged if compared with uncentrifugedanemicpatients blood specimens on blood type test slide method. 
Efek Homogenisasi Spesimen Darah Metode Inversi Terhadap Nilai Hematokrit Fadillah, Noor; Afriansyah, Muhammad Ardi; Sukeksi, Andri; Santosa, Budi
Jurnal Analis Kesehatan Vol. 12 No. 1 (2023): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v12i1.3714

Abstract

Laboratory examination consists of three stages, namely pre-analytical, analytical, and post-analytic. The biggest error occurred in the pre-analytical stage, which was around 61%. Homogenization including the pre-analytical stage is the process of mixing blood and anticoagulants which are included in the pre-analytical stage. Homogenization has two ways namely manual and automatic. Inadequate homogenization can cause clots or rupture of erythrocytes/lysis and shrinkage of erythrocytes, leading to a falsely low hematocrit value. The study aimed to find out and analyze differences in hematocrit values by homogenizing the inversion method 2, 4, and 10 times. This type of research is observational analytic. The research was carried out in June - August 2021 at the Clinical Laboratory of Muhammadiyah University of Palangkaraya. The research sample consisted of 9 EDTA blood from 9 people according to the inclusion criteria. All samples were examined for hematocrit using a Hematology Analyzer Sysmex XP-300. The results showed that the average hematocrit value in 2 times inversion homogenization was 38.40%, 4 times homogenization was 38.78%, and 10 times homogenization was 39.14%. The conclusion of this study was that there was no significant effect of the inversion homogenization technique 2, 4, and 10 times on the hematocrit value (p=0.584 > 0.05).
Co-Authors Afriansyah, Muhammad Ardi Agustin, Dela Putri Ana Hidayati Mukaromah Budi Santosa Budi Santosa Gela Setya Ayu Putri Handayani, Sri Puji Henna Ria Sunoko, Henna Ria Janah, Inayah Qori Nur Jimat, Resi Tondho Joko Teguh Isworo Kadarwati, Ari Maolida, Nurul Meturan, Dessy Marselina Meyliana, Adinda Noor Fadillah Nuroini, Fitri Pratama, Aji Sukma Puspitaningrum, Rioni Wahyu Ragil Saptaningtyas, Ragil Rahmawati, Rizqi Yogania Sari, Sri Mayang Stalis Norma Ethica Wahyuningtyas, Nunung Dartini Wulandari, Eka Wahyu Putri Wulandari, Try