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Endah Sulistiawati
Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Perbandingan Kinerja Ekstraksi Fikobiliprotein dari Spirulina platensis Melalui Pengadukan dan Freezing-Thawing Endah Sulistiawati; Martomo Setyawan; Zainal Abidin; Muhammad Darmawan; Harkris Alfian Makasar; Tegar Wahyu Pamungkas
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 1 (2023): Juni 2023
Publisher : Politeknik - Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v18i1.912

Abstract

Fikobiliprotein terdiri dari fikosianin, allofikosianin, dan fikoeritrin, merupakan komponen bioaktif yang terdapat pada Spirulina platensis (SP), berfungsi sebagai antioksidan dan antikanker. Metode ekstraksi secara pengadukan dan freezing-thawing dipilih karena sederhana, dan paling mudah untuk diterapkan pada skala industri. Penelitian ini membandingkan kinerja secara pengadukan dan freezing thawing pada ekstraksi fikobiliprotein (PBP) dari SP dengan solven air suling. Variabel yang diteliti adalah waktu pengadukan (1-30 menit), perbandingan solven terhadap biomassa (10-200 mL/g), waktu pembekuan (1-16 hari), serta kombinasi antara waktu pengadukan dan pembekuan. SP segar dan kering diperoleh dari CV. Paring Spirulina, Maguwoharjo, Sleman, Yogyakarta. SP segar (5 g) ditambah air suling (10-50 mL), diaduk dengan pengaduk magnetik (300 rpm), ekstraksi, dan penyaringan vakum. Filtrat yang dihasilkan diukur absorbansinya menggunakan spektrofotometer pada panjang gelombang 562, 615, dan 652 nm. SP kering (0,5 hingga 5,0 g) ditambah air suling, lalu diaduk seperti prosedur pada SP basah.  Metode freezing-thawing dengan pengadukan dimulai dengan pembekuan SP, dilanjutkan dengan thawing, pengadukan, dan penyaringan vakum. Waktu pengadukan 1-5 menit (tanpa pembekuan) memberikan hasil PBP 10,39±0,44 mg/g SP kering. Waktu pembekuan 16 hari (dengan atau tanpa pengadukan) memberikan hasil PBP yang relatif tinggi, yaitu 68,15 ± 2,89 mg/g SP kering. Perbandingan solven terhadap biomassa (S/B) optimum pada 100 mL/g SP kering.  Aktivitas antioksidan PBP yang ditunjukkan dengan nilai IC50 adalah 47,19 ± 0,04 mg/L (dari SP segar), dan 42,63 ± 0,08 mg/L (dari SP kering) pada kondisi ekstraksi yang optimum. AbstractPhycobiliprotein consisting of phycocyanin, allophycocyanin, and phycoerythrin, is a bioactive component found in Spirulina platensis (SP), which functions as an antioxidant and anticancer. Stirring and freezing-thawing were chosen as the extraction methods due to simple and easy to apply on an industrial scale. This study compared the stirring and freezing-thawing performance in extracting phycobiliprotein (PBP) from SP with distilled water as solvent. The variables studied were stirring time (1-30 minutes), solvent-to-biomass ratio (10-200 mL/g), freezing time (1-16 days), and the combination of stirring and freezing time. Fresh and dry SP obtained from CV. Paring Spirulina, Maguwoharjo, Sleman, Yogyakarta. Fresh SP (5 g) was added with distilled water (10-50 mL), stirred with a magnetic stirrer (300 rpm), extraction, and vacuum filtering. The absorbance of the filtrate was measured using a spectrophotometer at a wavelength of 562, 615, and 652 nm. Dry SP (0.5 to 5.0 g) was added with distilled water, then stirred like the procedure for wet SP. The freezing-thawing and stirring method began with SP freezing, followed by thawing, stirring, and vacuum filtering. Stirring for 1-5 minutes (without freezing) obtained PBP of 10.39 ± 0.44 mg/g dry SP. Freezing time of 16 days (with or without stirring) obtained relatively high PBP yields, namely 68.15 ± 2.89 mg/g dry SP. The optimum solvent ratio to biomass (S/B) was 100 mL/g dry SP. The antioxidant activity of PBP at the optimum condition, as indicated by the IC50 value, was 47.19 ± 0.04 mg/L (from fresh SP) and 42.63 ± 0.08 mg/L (from dry SP).
Pembuatan Serbuk Fikobiliprotein dari Spirulina platensis melalui Proses Freezing-Thawing dan Freeze-Drying Mazareta, Selva; Sulistiawati, Endah; Evitasari, Rachma Tia; Setyawan, Martomo; Hakika, Dhias Cahya
Eksergi Vol 21, No 3 (2024)
Publisher : Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31315/e.v21i3.12453

Abstract

Mikroalga golongan Cyanophyta seperti Spirulina Platensis (SP) merupakan salah satu sumber makanan fungsional yang bergizi. SP mengandung senyawa fikobiliprotein (PBP) yang merupakan komponen terbesar dari protein dan berfungsi sebagai antioksidan dan antikanker. Penelitian ini bertujuan untuk mengetahui pengaruh perbandingan solven-biomassa (S/B), waktu pengeringan beku, dan luas permukaan wadah sampel pada pengeringan beku terhadap serbuk PBP yang dihasilkan. Eksperimen diawali dengan proses perendaman SP kering (1,5 dan 3 g) selama 1 jam, pembekuan selama 24 jam, thawing, penyaringan vakum (30-90 menit) dan pengeringan beku (freeze-drying) pada filtrat yang diperoleh. Analisis konsentrasi PBP dalam filtrat dilakukan dengan mengukur absorbansinya menggunakan UV-Vis Spektrofotometer diuji pada panjang gelombang 562, 615, dan 652 nm.  Kadar air pada serbuk PBP setelah dilakukan proses freeze-drying diukur secara gravimeteri. Variasi yang dilakukan meliputi perbandingan S/B (6,67-133,33) mL air suling/g SP kering. Variasi waktu freeze drying selama 24 dan 32 jam. Waktu optimum untuk penyaringan vakum 30,55 menit dan menghasilkan konsentrasi PBP tertinggi (7,639 g/L). Nilai S/B optimum didapatkan  sebesar 20 mL/g dengan rendemen 112,32 mg/g (SP kering). Luas permukaan wadah sampel pada pengeringan beku yang terbaik adalah 427,5 cm2 dan banyaknya air yang teruapkan sebesar 99,71% dari berat filtrat
The Effect of Adding Bread Yeast, Tempeh Yeast, and Tape Yeast on The Process of Making VCO from Coconut Sinaga, Alexander Maruli Tua; Permadi, Adi; Sulistiawati, Endah; Totok Eka Suharto
Indonesian Journal of Chemical Engineering Vol. 2 No. 1 (2024)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/ijce.v2i1.665

Abstract

Coconut (Cocos nucifera L.) is one of Indonesia's agricultural products with potential. The most valuable coconut product is coconut oil, which can be obtained from the flesh of fresh coconuts or from copra. Virgin Coconut Oil (VCO) or pure coconut oil is the result of processing from the coconut plant in the form of a clear liquid with a distinctive coconut smell and has a long shelf life. Pure coconut oil or VCO has many benefits for body health, such as natural antibacterial, antiviral, anti-fungal and anti-protozoal properties. This study aims to compare the effect of tempe yeast, bread yeast, tape yeast, and without yeast on variations in the ratio of grated coconut and water (1: 1; 1:1,5; 1:2), on the amount of VCO produced. The experiment was carried out again and then the average volume of VCO produced by each yeast was taken. The average VCO results obtained ratio 1:1 were 209.5 ml for adding bread yeast, 153.5 ml for not using yeast, and 150.5 ml for adding tempeh yeast. Meanwhile, adding tape yeast produce the smallest yield 89 ml . Based on the ratio of grated coconut and water (1 kg: 1 liter) the optimum yield is 1:1. While the minimum yield is 1:1,5 ratio. The most VCO obtained is by adding baker's yeast to thick coconut milk in the second fermentation process. The order of highest yield based on the addition of yeast is bread yeast, tempeh yeast, and tape yeast. The yield of VCO yeast tempeh was slightly better than the yield without adding yeast. The organoleptic results and some of the VCO test results based on SNI 7783-2008 have fulfilled the specified requirements.
Karakteristik Tepung Kulit Pisang Modifikasi Menggunakan Hydrogen Rich Water budiarti, Gita Indah; Sulistiawati, Endah; Septiani, Nona; Septianindi, Windi
Jurnal Rekayasa Bahan Alam dan Energi Berkelanjutan Vol. 5 No. 1 (2021)
Publisher : Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rbaet.2021.005.01.05

Abstract

Banana peels are rich in fiber and antioxidants that are good for health. Banana peels can be used as flour. However, banana peel flour does not expand as well as flour. Starch modification is needed to improve the physicochemical properties of banana peel flour. Starch modification using Hydrogen Rich Water (HRW) is safer and cheaper than other methods. The novelty of this study is that there are no studies using HRW to modify banana peel flour. The purpose of this study was to determine the physical characteristics, reducing sugar content and protein content of HRW modified banana peel flour according to the standard SNI flour 3751: 2009. The main ingredients used are banana peels and HRW. Experiments were carried out by immersing banana peels in HRW for 15.30,45,60,75 minutes and pH (5 and 9). The results obtained were no changes in the physical characteristics of modified banana peel flour. The best results of sugar and protein content were obtained at pH 5. The best reducing sugar content at 60 minutes immersion was 2.1575%. The best protein content was obtained at 15 minutes immersion of 4.2152%.
Pelatihan Pemanfaatan Sampah Buah Menjadi Pupuk Cair Organik bagi Ibu-ibu PKK Kalurahan Murtigading Sanden Bantul Salamah, Siti; Hakika, Dhias Cahya; Sulistiawati, Endah; Amelia, Shinta; Rahmadewi, Yunda Maymana
Indonesia Berdaya Vol 3, No 3: May-July 2022
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.2022286

Abstract

Consumption of fruit is highly recommended to keep the body healthy. In line with increasing public awareness of fruit consumption, the amount of fruit waste also increases. One way to reusing fruit waste is to convert it into liquid organic fertilizer (LOF). A group of female senior citizen (PKK) from  Murtigading Village, Sanden, Bantul participated a workshop on making LOF reactors in the form of stacked buckets as an effort to convert fruit waste into plant fertilizer. LOF is made from fruit waste that is put into a stacked bucket reactor. The entire process takes two months to produce leachate, which is then aerated for one month before being used. The participants were pleased about the workshop and learned about the benefits of LOF 80% and how to make LOF 84%. 
Comparative study of ultrasonic and maceration extraction in enhancing antioxidant and SPF properties of green coffee beans serum Firdaus, Stradivary; Permadi, Adi; Sulistiawati, Endah
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/GAMCE9IN

Abstract

This study investigates the efficiency of maceration and ultrasonic extraction methods for obtaining antioxidants from Arabica, Robusta, and Liberica green coffee beans. Ultrasonic extraction demonstrated higher yields and enhanced antioxidant activity, with Liberica exhibiting the most potent radical scavenging potential (lowest IC50 values), followed by Robusta and Arabica in 45.3706 ppm, 46.6647 ppm, 49.4257 ppm. Formulated serums derived from these extracts were evaluated for compliance with SNI 16-4399-1996 standards. Both methods produced serums with acceptable texture, homogeneity, pH levels, and active ingredient retention. However, ultrasonic-derived serums displayed superior microbial safety profiles, with significantly lower total plate counts. Viscosity analysis revealed higher values for maceration-derived serums, while Sun Protection Factor (SPF) evaluation indicated that serum of Liberica extract provided the highest UV protection. These findings emphasize the potential of ultrasonic extraction and Liberica green coffee beans in developing high-value cosmetic and pharmaceutical products, paving the way for further research into optimized extraction techniques and broader applications.
DEKOLORISASI CRUDE RICE BRAN OIL MENGGUNAKAN BENTONIT Endah Sulistiawati; Arum Sari; Rafiqah Hidayati; Chaniago Chaniago
Spektrum Industri Vol. 10 No. 1: April 2012
Publisher : Universitas Ahmad Dahlan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/si.v10i1.1616

Abstract

Minyak bekatul atau Rice Bran Oil kaya akan gizi, mengandung vitamin, antioksidan (tokoferol, tokotrienol, dan orizanol), serta nutrisi yang dapat meningkatkan kualitas kesehatan manusia. Pada penelitian sebelumnya, telah dilakukan pembuatan minyak bekatul dengan proses fermentasi, hingga diperoleh minyak berupa crude oil (minyak kasar), yang masih berwarna gelap. Selanjutnya dilakukan upaya pemurnian dengan proses pemucatan (dekolorisasi). Penelitian ini bertujuan untuk mendapatkan angka perbandingan berat bentonit dengan minyak bekatul serta waktu pengadukan yang optimal, sehingga diperoleh warna minyak yang lebih pucat dari semula. Bentonit dengan ukuran lebih dari 200 mesh dimasukkan kedalam beker glass yang telah diisi minyak bekatul 50 ml dan magnetic stirrer pada suhu kamar tanpa pemanasan dengan kecepatan pengadukan 600 rpm selama 30 menit. Setelah pengadukan dihentikan, campuran diendapkan selama 24 jam lalu dilakukan pemisahan antara bentonit dengan minyak yang sudah jernih menggunakan kertas saring. Untuk menyempurnakan hasilnya dilakukan pemisahan dengan centrifuge. Perlakuan yang sama diulang untuk variabel berat bentonit (5, 10, 15, 20, 25 gram) dan waktu pengadukan (30, 40, 50 menit pada masing–masing berat). Parameter yang digunakan untuk menganalisa kualitas minyak bekatul pada penelitian ini adalah absorbansi (angka kejernihan relatif), kadar air, dan kadar kotoran. Dari hasil penelitian diperoleh berbagai tingkat kejernihan, hasil terbaiknya pada perbandingan berat bentonit terhadap minyak 0,5 g/ml, lama pengadukan 30 menit, memiliki angka kejernihan relatif 0,520. Kadar kotoran yang terendah terdapat pada perbandingan berat bentonit terhadap minyak 0,1 g/ml dan lama waktu pengadukan 40 menit, yaitu sebesar 0,028 %. Kadar air rata-rata minyak hasil dekolorisasi masih cukup besar yaitu 12,57%. Kata Kunci: dekolorisasi, rice bran, minyak, bentonit
DELIGNIFIKASI BAMBU PETUNG (DENDROCALAMUS ASPER) DENGAN EKSTRAK ABU JERAMI PADI DAN KAYU Endah Sulistiawati; Imam Santosa
Spektrum Industri Vol. 10 No. 2: Oktober 2012
Publisher : Universitas Ahmad Dahlan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/si.v10i2.1631

Abstract

Kebutuhan tekstil di Indonesia terus meningkat seiring dengan bertambahnya jumlah penduduk. Selama ini pemenuhan tekstil sebagian besar diimpor dari beberapa negara, antara lain: India, China, dan Jepang. Di sisi lain, Indonesia merupakan negara agraris, dengan limbah pertanian yang melimpah. Juga tanaman bambu tumbuh subur dan banyak terdapat di negeri ini. Keunggulan serat tekstil bambu adalah bersifat antiseptik, tahan terhadap mikroba. Penelitian ini merupakan salah satu langkah dalam pembuatan serat tekstil alami dari bambu petung (Dendrocalamus asper) dan limbah pertanian yaitu abu jerami padi dan kayu, yaitu tahap delignifikasi. Tujuan penelitian ini mencari waktu perendaman bambu dalam ekstrak abu yang memberikan hasil terbaik. Bambu dipotong dan dibelah tipis, berukuran panjang 15 cm, setebal 0,5 mm. Mula-mula sampel bambu ditimbang (antara 12 sampai 57 gram), lalu direndam dalam ekstrak abu (jerami padi dan kayu) sebanyak 500 ml dalam sebuah botol berkapasitas 600 ml dan ditutup. Perendaman dilakukan pada suhu kamar. Waktu perendaman bervariasi dari 4 jam hingga 80 jam. Setelah perendaman selesai, hasil disaring. Sampel filtrat dititrasi untuk diketahui konsentrasi alkali aktifnya. Bambu yang telah direndam lalu dikeringkan menggunakan oven pada suhu 110ºC sampai berat tetap. Selisih berat antara bambu awal dan akhir (kering) dihitung, dan dianggap sebagai lignin yang terdegradasi. Dari penelitian dapat disimpulkan bahwa perendaman menggunakan ekstrak abu jerami padi memberikan hasil yang lebih baik dari pada ekstrak abu kayu, karena pengurangan berat padatan lebih besar. Hasil yang tertinggi pada perendaman menggunakan ekstrak abu jerami padi selama 76 jam, dengan selisih berat (basis kering) mencapai 44,5%. Kata kunci: delignifikasi; bambu petung; ekstrak abu.
Qualitative and Quantitative Analysis of Lecithin Content and Bioactive Chemical Compenents of Corn Oil From Dompu District Ramadhan, M. Kaisar Sutomo; Permadi, Adi; Sulistiawati, Endah
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7494

Abstract

Indonesia is one of the Asian nations that has an abundance of vegetation that can be utilized as food yields and medication. One of the plants that can be utilized as neighborhood food is the Corn plant (Zea mays L.), corn is one kind of food crop that has for some time been known and developed. Dompu Regency, located in the province of West Nusa Tenggara,  is one of the largest corn producers in Indonesia. From this statement, a revolution is needed for the development of the food industry sector, namely the development of processed products from corn raw materials, namely corn oil. Corn oil extraction is carried out by the Maceration Method, a simple extraction process carried out by soaking the simplicia in a solvent for a certain time at room temperature and protected from light. Development in finding the percentage level (%) of lecithin LC-HRMS (Liquid Choromatography High-Resulution Mass Spectrometry), Among the Compounds: Percentage level (%) of bioactive lecithin compounds from major (main) components: 1-[(11Z)-octadecenoyl]-sn-glycero-3-phosphocholine (C26H52NO7P) = 8.882%, 1-Linoleoyl-2-Hydroxy-sn-glycero-3-PC (C26H50NO7P) = 6.874%, 1 Palmitoy-Phospadicholine (C24H50NO7P) = 1.898%, 1-Oleoyl-2-hydroxy-sn-glycero-3-PE (C23H46NO7P) = 1.161%, 2-monolinolenin (C21H36O4) = 0.577, (2R)-2-Hydroxy-3-(phosphonooxy)propyl (11Z,14E)-11,14-icosadienoate (C23H43O7P) = 0.386 %, 1-Stearoylglycerol (C21H42O4) = 0.072.
Pemanfaatan Ampas Kelapa Hasil Dapur Rumah Tangga Menjadi Pangan Olahan Rahmadewi, Yunda Maymanah; Hakika, Dhias Cahya; Sulistiawati, Endah; Salamah, Siti; Amelia, Shinta
Indonesia Berdaya Vol 4, No 4 (2023)
Publisher : UKInstitute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.2023561

Abstract

Coconut dregs often become waste and garbage due to the lack of knowledge and skills of the community in utilizing the existing potential. Even though, coconut dregs still contain fiber which is good for the body. Activities using educating and training methods aimed to increase the knowledge and skills of the community in processing coconut dregs into processed food. Educating can increase participants' knowledge by 28.33 points. Training on processing sago cheese (sagu keju) from coconut dregs flour can improve skills in products that need to be equipped with a brand so that they have competitiveness in the market.
Co-Authors Acnes Meyta Puspa Adi Permadi, Adi Agus Aktawan Agus Aktawan, Agus Amelia, Shinta Andinni Putri Winata APS, Yunizar Rizka Arum Sari Arya Aji Saka Ayu Wulandari Budiarti, Gita Indah Chaniago Chaniago Chaniago, Chaniago Delvi Aristianingsih Dessy Norma Yunita Dhias Cahya Hakika Erna Astuti Evitasari, Rachma Tia Firdaus, Stradivary Gita Indah Budiarti Harkris Alfian Makasar Hidayati, Rafiqah Ika Dyah Kumalasari Imam Santosa Imam Santosa Juwitaningtyas, Titisari Lukhi Mulia Shitophyta Mahsunah, Anis Herliyati Martomo Setyawan Martomo Setyawan Mazareta, Selva Mitha Ulli Rahmatillah Muhammad Darmawan Nurani Sofiana Purba, Irene Enjeli Qaulina Tsabit Rafiqah Hidayati Rahayu, Aster Rahmadewi, Yunda Maymana Rahmadewi, Yunda Maymanah Ramadhan, M. Kaisar Sutomo Riantika Aulia Putri Saka, Arya Aji Sarah Fazlia Sari, Arum Septiani, Nona Septianindi, Windi Setiyani, Seila Sinaga, Alexander Maruli Tua Siti Mutmaina Siti Salamah Siti Salamah Suhendra Suhendra Suhendra Suhendra Tegar Wahyu Pamungkas Totok Eka Suharto Tresya Pantoiyo Wahidah Mahanani Rahayu Winata, Andinni Putri Yunizar Rizka APS Zainal Abidin