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All Journal JURNAL HUTAN LESTARI JURNAL TEKNOLOGI LINGKUNGAN Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Seruni - Seminar Riset Unggulan Nasional Inoformatika dan Komputer Jurnal IPTEK Konversi BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL PERTANIAN Jurnal Leverage, Engagement, Empowerment of Community (LeECOM) Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society International Journal of Business, Law, and Education Abdimas Indonesian Journal Jurnal Ilmu Kehutanan Journal of Tourism, Culinary, and Entrepreneurship (JTCE)
Joko Sulistyo
Bagian Teknologi Hasil Hutan, Fakultas Kehutanan, Universitas Gadjah Mada
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Neuroscience Social Sciences Veterinary Other

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PURIFIKASI SENYAWA POLIFENOL GLUKOSIDA HASIL REAKSI TRANSGLIKOSILASI ENZIMATIK DARI BIAKAN DAN UJI AKTIVITASNYA SEBAGAI ANTIMIKROBA Handayani, Rini; Rahayu, Rita Dwi; Sulistyo, Joko
Jurnal Teknologi Lingkungan Vol. 13 No. 2 (2012)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jtl.v13i2.1420

Abstract

Polifenol-glukosida disintesis menggunakan CGTase yang berasal dari Nocardia sp. Sintesis polifenol-glukosida dilakukan dengan menggunakan resorsinol sebagai akseptor dan tepung sagu sebagai donor. Penelitian ini bertujuan untuk mensintesis senyawa polifenol-glukosida secara enzimatik menggunakan CGTase dari biakan Nocardia sp,menguji aktivitasnya sebagai senyawa antimikroba dan untuk mengetahui pengaruh senyawa polifenol-glukosida terhadap kerusakan morfologi sel dari biakan Bacillus subtilis. Polifenol–glukosida hasil reaksi transfer dimurnikan menggunakan kolom kromatografi yang berisi matriks oktadesil silica dan menggunakan eluen asam formatdalam methanol (40-90%). Produk yang sudah terpisah ditunjukkan sebagai noda tunggal pada plat kromatografi lapis tipis dengan nilai Rf mendekati nilai Rf standar arbutin. Nilai Rf dari produk transfer tersebut adalah sebesar 0,84 dan nilai Rf standar arbutin adalah sebesar 0.85. Polifenol-glukosida hasil sintesis menunjukkan aktivitasantimikroba terhadap biakan Bacillus subtilis dan Escherichia coli. Kata kunci : polifenol-glukosida, CGT-ase, Nocardia sp., Bacillus subtilis dan Escherichia coli AbstractPolyphenol-glucoside was synthesized by using CGTase derived from Nocardia sp. Synthesis polyphenol-glucoside of was done by using resorcinol as the acceptor and starch sago as the donor. This study aims to synthesized polyphenol-glucoside enzymatically using CGTase derived from Nocardia sp and to assay it’s activity as antimicrobial compound and to determine effect of polyphenol-glucoside on morphological damaging of Bacillus subtilis cells. The synthesized polyphenols-glucoside by transfer reaction was purified through column chromatography containing octadecyl silica matrix that was eluted with formic acid in methanol (40-90%). The separated product was demonstrated by single spot appearance on thin-layer chromatography plate with Rf value that was closed to standard of arbutin Rf. The Rf value of this transfer product was 0.84 while Rf value of arbutin as authentic standard was 0.85. The synthesized polyphenol-glucosie exhibited antimicrobial activity against Bacillus  subtilis and Escherichia coli. Key words : Polyphenol-glucoside, CGT-ase, Nocardia sp., Bacillus subtilis and  Escherichia coli.
EKSTRAKSI LEMAK SEL TUNGGAL MIKROBA YANG BERPOTENSI MENGHASILKAN BIODIESEL Sulistyo, Joko; Purwaningsih, Sri
Jurnal Teknologi Lingkungan Vol. 15 No. 1 (2014)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.631 KB) | DOI: 10.29122/jtl.v15i1.1452

Abstract

Beberapa biakan mikroba dan mikroalga dapat menghasilkan biomasa yang mengandung asamlemak, sehingga berpotensi sebagai sumber penghasil lemak sel tunggal. Pembuatan biodieseldari lemak sel tunggal dapat diproduksi dengan memodifikasi asam lemak melalui esterifikasi dantransesterifikasi menggunakan metanol serta basa atau asam kuat sebagai katalis. Tujuan daripenelitian adalah untuk mengeksplorasi potensi biakan mikroba dan alga sebagai penghasil lemaksel tunggal mulai dari penyiapan bahan, inkubasi, ekstraksi minyak, esterifikasi dan transesterifikasibiomasa secara tidak langsung. Transesterifikasi dengan pelarut heksan-isopropanol akanmenghasilkan kandungan metil ester asam lemak yang tinggi. Lemak sel tunggal yang diekstraksidari biakan kapang (Monascus purpureus, Monilia sitophyla, Aspergillus oryzae, Mucor javanicus,Fusarium oxysporum) dan alga (Chlorella vulgaris and Scenedesmus dimorphus) dapat menghasilkanbiodiesel melalui transesterifikasi secara langsung, menggunakan panas setelah penambahansejumlah banyak metanol anhidrat dan sodium hidroksida sebagai katalis yang berperan untukmeningkatkan kecepatan reaksi serta hasil yang diperoleh. Lemak sel tunggal pada kapang F.Oxysporum dan M. Javanicus didominasi oleh asam palmitat (41 % dan 40 %), sedangkan padakapang A. Oryzae, M. Purpureus dan M. Sitophyla didominasi oleh asam linoleat (42 %, 36,1 %dan 36 %). Sedangkan pada alga C. Vulgaris didominasi oleh asam linolenat (47 %), dan pada algaS. Limorphus didominasi oleh asam linoleat (35 %). Asam palmitat dan linoleat berpotensi untukmenghasilkan biodiesel melalui esterifikasi. Dimana, sejumlah banyak metanol digunakan untukmenghasilkan lebih banyak metil ester sebagai produk akhir.
BIOTRANSFORMASI PIROKATEKOL GLIKOSIDA MENGGUNAKAN KULTUR SUSPENSI SEL SOLANUM MAMMOSUM L. Soeka, Yati Sudaryati; Sulistyo, Joko
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.01 KB) | DOI: 10.24002/biota.v15i2.2704

Abstract

A syntesis of pyrocathecol glucoside was carried out by applying biotransformation cell culture suspension from calus of Solanum mammosum L., on modified medium of Murashige and Skoog (MS). A growth maximum volume at 15,5 ml of cell culture suspension of S. mommosum was achieved on day-8 incubation. The results showed that pyrocathecol glucoside as a bitransformation product that was obtained by application of Pyrocathecol at 50-200 ppm was determined by TLC and identified at Rf value of 0.82?0.83. Futhermore, the biotransformation products were determined by HPLC obtained from the cell culture suspension at concentration of 200 ppm pyrocathecol so that resulted in reaction products based on standard solution. The peaks number 1, 2 and 3 with retention time 2.53 min, 4.62 min and 7.58 min were appropriate to the retention time cellobiose, glucose and methyl ?-glucoside, respectively. Peak number 4 with retention time 8.52 min conformed to pyrocathecol-glucobioside as a product of side transfer and peak number 5 with retention time 10.52 min in line with the retention time of arbutin were pyrocathecol-glucoside as a transfer product expected from the result of biotransformation.
BIOLOGICAL ACTIVITY OF ENZYMATICALLY SYNTHESIZED POLYPHENOL GLYCOSIDE ON MICROBIAL GROWTH Soeka, Yati Sudaryati; Widiasih, Lutfi Erlita; Sulistyo, Joko
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3467

Abstract

ABSTRACTBiological Activity of Enzymatically Synthesized Polyphenol Glycoside on Microbial Growth. We have studied an indigenous bacterial strain produced a glycosyl transfer enzyme (CGTase) yielding polyphenol glycosides from a substrate of starch and polyphenol-aglycone. We observed that the CGTase derived from culture filtrate of some microbial strains (Candida rugosa, Bacillus megaterium, B. coagulans and B. polymixa) could synthesize transfer products in the presence of appropriate polyphenol-aglycones as their acceptors. An inhibitory effects of enzymatically synthesized polyphenol glycosides against bacterial growth was furthermore examined. It was found that polyphenol-glycoside, as one of the transfer products, exhibited high antibacterial activity on the growth of Bacillus subtilis and Escherichia coli, no effect when on Bacillus cereus.Key words : Cyclodextrin glucano transferase (CGTase), enzymatic transglycosylation,polyphenol glycoside, antibacterial activity.
PENGEMBANGAN INOVASI KECAP AROMATIK BEBAS AFLATOKSIN DI DESA BALONG TUNJUNG, KECAMATAN BENJENG, KABUPATEN GRESIK Sulistyo, Joko; Dewi, Irra Chrisyanti; Yoewono, Jessica Renata
Jurnal Leverage, Engagement, Empowerment of Community (LeECOM) Vol. 4 No. 2 (2022): Jurnal Leverage, Engagement, Empowerment of Community (LeECOM)
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/leecom.v4i2.3503

Abstract

Untuk mencegah kontaminasi aflatoksin dalam produksi kecap tradisional di Indonesia, ragi kecap dari biakan kapang mutan Aspergillus oryzae yang berspora putih, dipersiapkan dari biakan kapang induk A. oryzae yang berspora hijau, melalui teknik iradiasi dengan sinar UV pada intensitas tertentu, sehingga diperoleh ragi kecap mutan yang tidak memproduksi aflatoksin dan dapat digunakan sebagai ragi kecap dalam rangka pengembangan produk kecap aromatik bebas aflatoksin di Desa Balong Tunjung, Kecamatan Benjeng, Kabupaten Gresik. Proses pembuatan kecap aromatik bebas aflatoksin dilakukan melalui tiga tahapan utama, yaitu fermentasi ragi (koji) yang dipersiapkan melalui inokulasi ragi kecap mutan pada kacang kedelai yang telah direndam, dikuliti dan dimasak selama 3–5 hari, dilanjutkan dengan fermentasi garam (moromi) berupa perendaman koji pada kadar garam tinggi (18–22%) selama 1–6 bulan, dan selanjutnya dilakukan pengolahan moromi dengan penambahan rempah-rempah aromatik serta pengemasan produk kecap. Hasil fermentasi koji, menunjukkan pertumbuhan miselia kapang berspora putih secara merata pada permukaan kedelai, menunjukkan proses penguraian secara enzimatik protein secara optimal, sehingga mempercepat proses penguraian bahan-bahan organik yang terkandung dalam kedelai selama fermentasi moromi, sehingga meningkatkan kualitas nutrisi. Hasil tersebut menunjukkan bahwa penggunaan ragi kecap mutan berspora putih tidak hanya berkontribusi pada pencegahan kontaminasi aflatoksin tetapi juga meningkatkan kualitas nutrisi pada produk akhir kecap.
VARIASI AKSIAL DAN RADIAL DIMENSI SERAT, SIFAT FISIKA DAN MEKANIKA KAYU JABON (Neolamarckia cadamba Miq.) DI HUTAN RAKYAT KABUPATEN SLEMAN, YOGYAKARTA Ridho, Muhammad Rosyid; Marsoem, Sri Nugroho; Listyanto, Tomy; Sulistyo, Joko
JURNAL HUTAN LESTARI Vol 12, No 1 (2024): JURNAL HUTAN LESTARI
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jhl.v12i1.76292

Abstract

Utilization of fast-growing wood species needs to be increased to support the sustainability of wood industry in Indonesia. Burflower (Neolamarckia cadamba Miq.) is a fast-growing wood species that widely planted in some community forest on Java Island recently, including in Yogyakarta. This study aims to provide information about burflower wood quality, based on its fiber dimension, physical and mechanical properties. The axial and radial variation of wood properties within stem in the axial and radial direction also examined. Three (3) free defects of burflower trees from community forest in Sleman regency, Yogyakarta, have been felled and cut into samples. The results show that green moisture content, air-dried density, and oven-dried density were varied in the axial direction. Meanwhile in the radial direction, fiber length, density, and static bending strength increase from the pith towards the bark. In contrast, green moisture content decreases from the pith towards the bark.Keywords: density, fast-growing, fiber length, modulus of elasticity, shrinkage. AbstrakPemanfaatan jenis kayu cepat tumbuh perlu ditingkatkan untuk mendukung kelanjutan industri perkayuan di Indonesia. Jabon (Neolamarckia cadamba Miq.) adalah salah satu jenis kayu cepat tumbuh yang banyak ditanam di hutan rakyat Pulau Jawa pada beberapa tahun terakhir, termasuk di Yogyakarta. Penelitian ini bertujuan untuk memberikan informasi terkait kualitas kayu jabon di hutan rakyat, berdasarkan dimensi serat, sifat fisika dan mekanikanya. Variasi aksial dan radial sifat kayu dalam satu batang pohon juga diamati. Sebanyak 3 batang pohon jabon bebas cacat dari hutan rakyat di Kabupaten Sleman, Yogyakarta, ditebang dan dibuat sampel uji. Hasil pengamatan menunjukkan bahwa kadar air kering udara (KAKu), kerapatan kering udara (KKu), dan kerapatan kering oven (KKo) bervariasi pada arah aksial. Sementara itu pada arah radial, variasi terlihat pada panjang serat, kerapatan, dan kekuatan lengkung statis yang menunjukkan pola peningkatan dari empulur menuju kulit. Sebaliknya, kadar air segar menurun dari empulur menuju kulit.Kata kunci: cepat tumbuh, kerapatan, modulus elastisitas, panjang serat, penyusutan
Physicochemical properties of fortified flour based modified cassava flour blended with bacterial poly-glutamic acid Sulistyo, Joko; Yu, Shin Ying; Heroney, Hevenney Vianie
Bahasa Indonesia Vol 3 No 2 (2023): OCTOBER 2023
Publisher : School of Tourism, Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/jtce.v3i2.4204

Abstract

Cassava is one of the most important staple crops worldwide. However, cassava flour needs to meet the high-quality requirements in terms of physicochemical characteristics. The present study was designed to investigate properties of fortified cassava flour produced from co-processing of modified cassava flour with poly-glutamic acid (PGA) derived from protein of beans that had been fermented by Bacillus natto. Proximate analysis, cyanide content, swelling power, solubility, and viscosity of modified cassava flour (MCF) which was fortified with poly-glutamic acid (PGA) was found to indicate improvements as compared to the native flour. The modified flours were further investigated for their physicochemical properties after addition of poly- -glutamic acid ( -PGA) at different levels. All flour samples showed no significant (p>0.05) differences in terms of lightness (L*), while greenness to redness (a*) of native flours was significantly (p<0.05) higher than modified flours with -PGA. There were significant (p<0.05) differences in the swelling power and solubility measured at various temperatures. From the pasting profiles, there were significant (p<0.05) increases in peak viscosity, final viscosity and pasting temperature of cassava flours due to addition of -PGA. Observation by scanning electron microscopy (SEM), pronounced cracks were observed in starch granules indicative of enzyme attack. It indicated that starch granules of modified and fortified cassava flour were depolymerized by enzymatic hydrolysis which led to cause change and degrade exterior surface of the granules within corrosion via pores of small granules.
Characterization of virgin coconut oil fermented using starter culture prepared with probiotic bacterial strain Sulistyo, Joko; Ramli, Adilah Md
Bahasa Indonesia Vol 4 No 1 (2024): APRIL 2024
Publisher : School of Tourism, Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/jtce.v4i1.4635

Abstract

Fermented Coconut Oil (FCO) stands out as a unique form of pure coconut oil, processed through fermentation with a starter culture. Primarily composed of medium-chain saturated fatty acids, it inherits the biological properties of coconut oil, extensively studied for its remarkable antibacterial potential. The high concentration of medium-chain fatty acids, particularly lauric acid and its monoglyceride derivative, monolaurin. endows FCO with potent antibacterial properties. This enables FCO to combat a range of pathogenic microorganisms effectively. This study investigated the antibacterial activity of FCO against cultured pathogens, including Listeria monocitogenes, Bacillus cereus and Salmonella typhimurium. The results revealed the ability of FCO to inhibit the growth of these pathogens. Regarding in vivo testing with mice, parameters such as weight gain, blood sugar, cholesterol. and triglyceride levels were assessed in blood serum. Histopathological examination of the spleen, liver, kidneys, and intestines did not reveal any adverse changes. However, mice infected with Escherichia coli and simultaneously given FCO showed focal necrosis in the liver due to E. coli infection. Observation of kidney tissue showed glomerular swelling and renal tubular epithelial necrosis in some groups, but did not appear in the control group. In particular, the group infected with E. coli and given FCO showed glomerular swelling and renal tubular epithelial cell necrosis. This shows the potential of FCO in reducing bacterial infections and highlights its impact on kidney health by preventing inflammation and necrosis in the kidneys.
Pengembangan Tempe Fantasy dengan Penambahan Pewarna Alami dan Rempah-Rempah Mengandung Antioksidan Sulistyo, Joko; Dewi, Irra Chrisyanti; Oktavio, Adrie
Abdimas Indonesian Journal Vol. 4 No. 1 (2024)
Publisher : Civiliza Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59525/aij.v4i1.333

Abstract

Color is one of the most relevant organoleptic attributes that directly influence consumer acceptance and food selection. Natural dyes play an important role in the food industry, not only because of their aesthetic appeal but also their health benefits. Natural dyes offer a variety of health-promoting properties, such as antioxidant, antimicrobial, and anti-inflammatory effects. Therefore, this important characteristic is the reason for adding coloring to develop a tempeh product namely Tempeh Fantasy. This research aims to determine the functional properties, enamelyspecially the antioxidant activity of tempeh products that have been added with natural colorings and aromatic ingredients. Through community service activities, research, and training, the development of tempeh-fantasy innovation has been carried out, which involves soaking cooked soybeans in combination solutions of butterfly pea flower and star anise powder, roselle flower and cinnamon, moringa leaf extract and ginger powder, turmeric and garlic extract, as well as Pangium nut and black garlic solution, then adding tempeh starter and further fermenting it. The results showed that the addition of natural colorings and herb-spices to tempeh succeeded in increasing its antioxidant activity. Additionally, the addition of natural colorings and spices to tempeh can enhance its exotic color and attractive aroma.
Pengaruh Penambahan Ekstrak Buah Duwet dan Bunga Mawar pada Kemasan Pintar Berbasis Selulosa Bakteri sebagai Indikator Kerusakan Makanan Winata, Kezia Jasmine; Anggara, Jaclyn Regina; Angkadjaja, Belinda Manuela; Gunawan, Fayola Feivel; Sulistyo, Joko
Abdimas Indonesian Journal Vol. 4 No. 2 (2024)
Publisher : Civiliza Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59525/aij.v4i2.409

Abstract

Despite its environmental and health drawbacks, plastic continues to be widely used because of its cost-effectiveness and versatility. This study investigates the potential of biodegradable plastic made from cellulose derived from nata de coco, which is produced through the fermentation of Acetobacter xylinum bacteria, as a sustainable alternative to conventional non-biodegradable plastics. The structural integrity of the bioplastic was improved by adding cassava starch, glycerin, and CMC. Additionally, extracts from rose and Java plum were incorporated into the material to serve as indicators of food spoilage. Functionality tests revealed that the bioplastic containing rose extract demonstrated the most significant color change, likely due to the degradation of anthocyanins present in the extract. This research presents a promising approach to addressing plastic pollution and reducing food waste through the use of biodegradable smart packaging.
Co-Authors Adrie Oktavio Anggara, Jaclyn Regina Angkadjaja, Belinda Manuela Evi Triana Fia Sri Mumpuni Gaby, Cecilia Gunawan, Fayola Feivel Hamidy, Raymond Heroney, Hevenney Vianie Hesti Wijayanti Irra Chrisyanti Dewi Jacob, Alexander Michael Joko Sulistyo Muarif, Muarif Muarif, Muarif Muhammad Rosyid Ridho Naiola, Elidar Napitupulu, Rostiati NR Nikkuni, Sayuki Nyoman Puspa Asri Oki Krisbianto Praharnata, Praharnata Purnama Dewi R. Sapto Hendri Boedi Soesatyo Rachman, Hans Rahmaningsih, Heni Ramli, Adilah Md Rini Handayani Rita Dwi Rahayu Sri Nugroho Marsoem Swee Ling, Joanna Ho Tomy Listyanto Widiasih, Lutfi Erlita Widiasih, Lutfi Erlita Winata, Kezia Jasmine Yati Sudaryati Soeka Yoewono, Jessica Renata Yu, Shin Ying