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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Buletin Peternakan Jurnal Veteriner Panrita Abdi - Jurnal Pengabdian pada Masyarakat Animal Production : Indonesian Journal of Animal Production Tropical Wetland Journal Journal of Animal Research in Wetland Ecosystem
Ika Sumantri
Department Of Animal Science, Faculty Of Agriculture, Universitas Lambung Mangkurat, Banjarbaru 70714
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Journal : Buletin Peternakan

 1 
KAPASITAS DAN STABILITAS PENGIKATAN BEBERAPA ADSORBEN AFLATOKSIN ALAMI DI DALAM RUMEN IN VITRO Ika Sumantri; Tridjoko Wisnu Murti; Joseph Boehm; Ali Agus
Buletin Peternakan Vol 36, No 3 (2012): Buletin Peternakan Vol. 36 (3) Oktober 2012
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v36i3.1623

Abstract

Penelitian ini dilaksanakan untuk menguji kapasitas dan stabilitas beberapa adsorben alami dalam mengikat aflatoksin B1 (AFB1) dengan metode in vitro yang melibatkan kondisi di dalam rumen. Percobaan in vitro dilakukan dengan menggunakan 3 macam adsorben alam (bentonit, zeolit dan karbon aktif) dan satu adsorben produk komersial (CAA). Pengujian dilakukan pada 3 macam medium in vitro (aquades steril, cairan rumen dan cairan rumen steril) dan 2 rasio AFB1:adsorben (1:1000 dan 1:10.000 yang setara dengan 1000 mg AFB1-contaminated feed:1 mg adsorben dan 100 mg AFB1-contaminated feed:1 mg adsorben). Inkubasi dilakukan selama 2 jam dengan penggojokan 70 rpm pada suhu 38,5oC menggunakan shaking incubator. Medium kemudian disentrifugasi selama 15 menit pada 3500 g. Supernatan diambil untuk dianalisis kadar AFB1 yang tidak terikat oleh adsorben, sehingga persentase AFB1 terikat dapat dihitung sebagai peubah kapasitas pengikatan. Presipitat selanjutnya diresuspensi dan diinkubasi kembali. Supernatan yang diperoleh setelah sentrifugasi 3500 g selama 15 menit dianalisis kandungan AFB1-nya untuk mendapatkan persentase AFB1 yang terlepas dari ikatan dengan adsorben untuk mendapatkan data stabilitas pengikatan. Data dianalisis variansi dengan prosedur general linear model rancangan acak lengkap pola faktorial menggunakan SPSS versi 17.0. Hasil memperlihatkan bahwa bentonit memiliki kapasitas pengikatan AFB1 tertinggi (77,54%) dengan medium aquades. Stabilitas pengikatan tertinggi ditunjukkan oleh CAA (99,78%) yang tidak berbeda dengan stabilitas bentonit (99,38%). Pengikatan AFB1 secara nyata (P<0,05) dipengaruhi oleh pH medium dengan kapasitas tertinggi diperoleh pada pH medium kurang dari 5,0. Berdasarkan peubah kapasitas dan stabilitas pengikatan serta pH optimum pengikatan dapat disimpulkan bahwa bentonit merupakan adsorben alami yang paling potensial untuk dipergunakan pada ternak ruminansia.(Kata kunci: Adsorben aflatoksin, Metode in vitro rumen)
AFLATOXINS CONTAMINATION IN FEED AND PRODUCTS OF ALABIO DUCK (Anas platyrinchos borneo) COLLECTED FROM SOUTH KALIMANTAN, INDONESIA Ika Sumantri; Ali Agus; Bambang Irawan; Habibah Habibah; Nur Faizah; Kharisma Julia Wulandari
Buletin Peternakan Vol 41, No 2 (2017): BULETIN PETERNAKAN VOL. 41 (2) MEI 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i2.15514

Abstract

A limited survey was conducted to determine aflatoxins contaminations in feed and products of Alabio duck. A total of 271 feed samples, 48 liver sample, 42 meat samples, and 38 egg samples were analyzed for determination of aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) using Enzyme-Linked Immuno-Sorbent Assay (ELISA) tests. Results showed high prevalence of AFB1 contamination in feed sample, especially in feed concentrate, dried fish, and rice hulls (100%). AFB1 concentrations were higher than tolerable limit for AFB1 in feed for laying duck according to Indonesia National Standard (SNI), being 20 ppb, except for sago pit. This survey also showed high prevalence and levels of aflatoxin residues in the products of Alabio duck. AFB1 was found in all liver samples, with concentrations ranging from 4 to 12 ppb (average: 7 ppb). AFM1 was found in all of liver, meat, and egg samples. The highest level of AMF1 was found in liver which was ranging from 105 to 1,215 ppt (average: 304 ppt). High level of AFM1 was also found in meat, namely between 71 to 128 ppt (averaged: 91 ppt). Although found at low level, AFM1 was detected in egg, which was ranging from 10 to 36 ppt (average: 19 ppt). This survey showed high contaminations of aflatoxins in the liver, meat and egg of Alabio duck collected from the area of survey and their concentrations of aflatoxins were harmful for the consumer.
Effect of Phyllosilicates As Toxin Binder On Productivity, Intestinal Morphology, and Liver Toxicity in Broiler Fed Afb1 Contaminated Feed Gardika Windar Prahara; Adhe Humaera; Lies Mira Yusiati; Chusnul Hanim; Asih Kurniawati; Ika Sumantri; Ali Agus; Erika Kusumawardani; Muhsin Al Anas
Buletin Peternakan Vol 47, No 2 (2023): BULETIN PETERNAKAN VOL. 47 (2) MAY 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i2.81410

Abstract

Aflatoxin B1 is a toxin produced by the fungus Aspergillus flavus which reduces the development and function of organs in broilers. The aim of this study was to determine the effect of adding binder toxin from different bentonite to feed contaminated with AFB1 on productivity, intestinal morphology, and liver toxicity in broilers. A total of 60-day old chick male broilers were placed in 12 pens. Each treatment consisted of three replicates, each replicate containing five broilers. Treatment in the study consisted of P0 (control, basal diet, without the addition of AFB1), P1 (P0 + 100 µg/kg AFB1 + 4 g/kg calcium bentonite Type A), P2 (P0 + 100 µg/kg AFB1 + 4 g/kg calcium bentonite Type B), and P3 (P0 + 100 µg/kg AFB1 + 4 g/kg calcium bentonite Type B + kerolite + saponite). Treatment diets were given to broilers from day 22 to 35 (finisher phase). The results showed that the toxin binder on AFB1 contaminated feed had no effect on feed consumption, body weight gain and feed conversion (p>0.05). Addition of toxin binder on AFB1 contaminated feed increased the relative weight of the duodenum (p=0.024), although P3 was not significantly different. Treatments had no effect on villus length, crypt depth, and ratio of villus length to crypt depth (p>0.05), but decreased villus width (p=0.013). The addition of toxin binder tended to decrease the villus area (p=0.055). SGOT and SGPT did not show differences between treatments. AFB1 contamination with the addition of toxin binder showed signs of toxicity on liver histopathological observations. Based on the research, it can be concluded that the addition of binder toxin in feed contaminated with AFB1 can reduce the negative effect on the development of intestinal villus and chemical effect to the liver. Toxin binder Type B has the best efficacy for reduce the negative effect
Co-Authors Abrani Sulaiman Achmad Syamsu Hidayat Adhe Humaera Ali Agus Ali Agus Ali Agus Ali Agus Ali Agus Anis Wahdi Asih Kurniawati Bahtiyar, Saiul Bambang Irawan Chusnul Hanim Erika Kusumawardani Fitri Purwanti Gardika Windar Prahara Habibah Habibah Hadian, Gloria Rida Herliani Herliani Herliwati Herliwati Joseph Boehm Kharisma Julia Wulandari Kuni Irfah Lies Mira Yusiati Lilis Hartati M. Rizki Amin Badali Mijani Rahman Misliani, Yesi Muhammad Riyadhi MUHAMMAD RIZAL Muhsin Al Anas Nur Faizah Nursyam Andi Syarifuddin Nuryono Nuryono Parwanto Rifani, Muhammad Ade Rizqiana, Sista Ronny Kurniawan Sholih Nugroho Hadi Sigit PRASTOWO Sista Rizqiana Tridjoko Wisnu Murti Ulil Amri Widi, TriSatya Mastuti Yesi Misliani