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Supplementation of Rumen By Pass Protein-Fat: Effect on Feed Intake, Nutrient Digestibility and The Profile of Duodenal Digesta Fatty Acids Hartati, Lilis; Sumantri, Ika; Agus, Ali
ANIMAL PRODUCTION Vol 16, No 2 (2014): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract.  The aim of this research was to study the effects of rumen by pass protein-fat supplementation (RBPF) on feed intake, nutrients digestibility and profile of duodenal digesta of fatty acids. Two rumen fistulated and duodenum canulated dairy cows were fed ration that consisted of king grass and concentrate (60:40). The concentrate was supplemented with 20% RBPF. The feeding trial was conducted for four weeks in which the first week was the control period, the second week was the adaptation period, the third week was the early collection period and finally the fourth week was the late collection period. The samples of duodenal digesta were collected 1 hour before morning feeding and 1, 3 and 5 hours after morning feeding. The cows were fed twice a day and concentrate was offered before forage. The results showed that in comparison to the control, supplementation of RBPF increased nutrients intake of dry matter (DM) (6.95 vs. 6.27%), organic matter (OM) (6.20 vs. 5.70%), crude fat (CF) (0.49 vs. 0.41%), crude fiber (CFb) (1.84 vs. 1.49%) and nitrogen free extract (NFE) (3.23 vs. 3.03%), while the crude protein (CP) intake decreased (0.64 vs. 0.77%). Supplementation of RBPF improved the nutrients digestibility of dry matter (67.24 vs. 62.15%), organic matter (68.71 vs. 65.29%), crude fiber (66.20 vs. 57.46%) and nitrogen free extract (72.37 vs. 66.47%). RBPF supplementation also increased linoleic acid content in duodenal digesta sample at 1and 3 hours after feeding. In conclusion, RBPF supplementation did not negatively affect feed intake and nutrient digestibility. RBPF also increased the proportion of linolenic acid, eicosapentaenoic acid (EPA) and docosahexsaenoic acid (DHA) in duodenal digesta until 3 hours after feeding. Key words: RBPF, supplementation, feed intake, nutrient digestibility, fatty acid Abstrak.  Penelitian ini bertujuan untuk mengetahui pengaruh  suplementasi protein-lemak by pass rumen terhadap konsumsi, kecernaan nutrien serta profil asam lemak pada digesta duodenum sapi perah. Dua ekor sapi perah berfistula rumen dan berkanula duodenum diberi pakan hijauan dan konsentrat dengan rasio 60:40, diberi suplemen protein-lemak by pass rumen 20% dari konsentrat.  Percobaan dilakukan selama 4 minggu, minggu pertama adalah periode kontrol, minggu ke-2 periode adaptasi, minggu ke-3 periode koleksi awal dan minggu ke-4 periode koleksi akhir. Sampel digesta duodenum diambil 1 jam sebelum pemberian pakan dan 1; 3 serta 5 jam setelah pemberian pakan. Ransum diberikan dua kali sehari dengan konsentrat lebih dulu sebelum hijauan. Dibanding periode kontrol, suplementasi protein lemak by pass rumen sebanyak 20% menaikkan konsumsi bahan kering (BK) (6,95 vs 6,27%), bahan organik (BO) (6,20 vs 5,70%), lemak kasar (LK) (0,49 vs 0,41%), serat kasar (SK) (1,84 vs 1,49%) dan bahan ekstrak tanpa nitrogen (BETN) (3,23 vs 3,03%) tetapi menurunkan konsumsi protein kasar (PK) (0,64 vs 0,77%). Suplementasi protein lemak by pass rumen juga memberikan kecernaan BK, BO, SK dan BETN ( 67,24 vs 62,15%; 68,71 vs 65,29%; 66,20 vs 57,46%; 72,37 vs 66,47%) yang lebih tinggi dibanding kontrol. Suplementasi lemak  by pass rumen juga menaikkan proporsi asam lemak linolenat pada jam ke-1 dan ke-3 setelah makan. Pemberian protein-lemak by pass rumen dalam ransum tidak berpengaruh negatif terhadap konsumsi dan kecernaan nutrien pakan serta meningkatkan proporsi asam lemak linoleat, EPA (eicosapentaenoic acid) dan DHA (docosahexsaenoic acid) digesta duodenum. Kata kunci: protein lemak by pass rumen, suplementasi, konsumsi, kecernaan nutrien, asam lemak
In Vitro Digestibilities of Six Rumen Protected Fat-Protein Supplement Formulas Hartati, Lilis; Agus, Ali; Widyobroto, BP; Yusiati, LM
ANIMAL PRODUCTION Vol 14, No 1 (2012): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The aim of the research was to evaluate the efficacy of protection method of rumen protected fat-protein supplements. In vitro digestibility test was carried out to examine nutrients digestibility of different supplement formula based on the sources of protein and oil. The research used two sources of fat namely crude palm oil (CPO) and fish oil (FO) and three sources of protein namely milk skim, soy flour, and soybean meal. Thus there were 6 combinations that subjected in the in vitro digestibility test. The observed variables were the digestibility of dry matter (DM), organic matter (OM), crude fat (CF), and crude protein (CP). Results indicated that the method for protecting protein and fat was effective. This was showed by low nutrients digestibility in the rumen and high nutrients digestibility in the post rumen. In conclusion the combination between skim milk and CPO gave the best results among the other supplement formula.Keywords: rumen protected nutrient, fat-protein supplement, rumen digestibility, in vitroAnimal Production 14(1):1-5, January 2012
Physicochemical Characteristics Identification and Secondary Metabolite Analysis of Solid Herbal Waste as Source of Feed Rich Fiber and Supplement for Ruminants Kisworo, Arif Nindyo; Agus, Ali; Kustantinah, Kustantinah; Suwignyo, Bambang
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (724.611 KB) | DOI: 10.20884/1.anprod.2016.18.2.535

Abstract

A study was conducted to determine the potency, physicochemical characteristics, and analyzed the secondary metabolites content of solid herbal waste (SWH) as a substitute source of feed rich fibre and feed supplements in ruminants. The first study includes an analysis of production potential, physical analysis, and chemical composition analysis of SWH. The second study was an analysis of secondary metabolites content of SWH. The results showed that SWH volume reached 6-8 m3/day or 4020-5360 kg/day, the chemical composition of herbal solid waste was similar to king grass with high lignin content (17.53%). SWH containing total phenols, flavonoids, tannins, saponins , alkaloids and essential oils which generally have antimicrobial activity. According to the potential availability, chemical composition and secondary metabolites content of SWH, it can be used as an alternative for substitute of feed rich fiber or feed supplements with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen. Further in vitro and in vivo research ore needed to determine the effect of SWH on rumen fermentation parameters and its application in the ration in ruminant livestock.
Free Fatty Acid Concentration and Carboxy methyl cellulase Activity of Some Formulas of Protected Fat-proteins Tested In Vitro Hartati, Lilis; Agus, Ali; Yusiati, Lies Mira; Widyobroto, Budi Prasetyo
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.311 KB) | DOI: 10.20884/1.anprod.2015.17.2.513

Abstract

The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and  cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of  free fatty acid concentration in fisrt stage in vitro (0.168%) and the highest result free fatty acid concentration in second stage in vitro ( 4.312%) . The activity of CMC-ase was not influenced by different  sources of fat and protein. It can be concluded was that the protection of the combination between skim milk and CPO gives the highest protection results.
Quality Evaluation of Complete Feed with Ramie Waste Added With Different Protein Source in Nutrient Composition and Ensilage Parameter Susanti, Emmy; Agus, Ali; Suraningdyah, Yuni; Suhartati, FM
ANIMAL PRODUCTION Vol 17, No 1 (2015): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.071 KB) | DOI: 10.20884/1.anprod.2015.17.1.480

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Abstract.  The objective of this research was to evaluate the effect of ensilage technology (with and without ensilage) and protein sources (plant and animal) in complete feed with ramie-waste on nutrient composition and ensilage parameter. Ensilage process decreased significantly dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF), but it increased  ensilage parameter i.e. acetate acid (C2), propionic acid (C3), butyric acid (C4), lactic acid (LA), and decreased pH. It also had non-significant effect on crude fibre (CP), acid detergent fiber (ADF) and ammonia (NH3-N). Animal protein source in silage making decreased nutrient composition i.e. OM and CP and ensilage parameter i.e. C2, but it increased NH3-N.  Interaction between ensilage treatment and protein sources affected CP and pH also ensilage parameter of C2 and C3. In conclusion, plant protein used in silage of complete feed with ramie waste was better than animal protein, despite that both ensilage were not better due to pH over 4.5. Key words: ramie, ensilage technology, protein source, nutrient composition, ensilage parameter Abstrak. Penelitian ini bertujuan mengevaluasi pengaruh teknologi ensilage (dengan dan tanpa ensilage) dan sumber protein (nabati dan hewani) pada complete feed menggunakan limbah rami terhadap komposisi nutrien dan parameter ensilage. Perlakuan teknologi ensilage menurunkan komposisi nutrien pada bahan kering (BK), bahan organik (BO) dan neutral detergent fiber (NDF, meningkatkan kuantitas parameter ensilage pada asam asetat (C2), asam propionat (C3), asam butirat (C4) dan asam laktat (LA) dan menurunkan pH serta tidak nyata pada protein kasar (PK), acid detergent fiber (ADF) dan ammonia (NH3-N).  Perlakuan sumber protein menurunkan nutrien BO dan PK dan C2 serta meningkatkan konsentrasi NH3-N secara sangat nyata pada complete feed dengan protein hewani. Interaksi kedua perlakuan berpengaruh pada PK dan pH serta C2 dan C3. Kesimpulannya adalah penggunaan protein nabati pada silage complete feed menggunakan limbah rami lebih baik dari pada yang menggunakan protein hewani meskipun kedua jenis silage tidak lebih baik karena pH lebih dari 4,5. Kata kunci: rami, teknologi ensilage, sumber protein, komposisi nutrien, parameter ensilage
Effect of Undegraded Protein and Energy Level on Intake and Digestibility of Nutrient and Blood Metabolite in Dairy Cows Widyobroto, BP; Budhi, SPS; Agus, Ali
ANIMAL PRODUCTION Vol 10, No 2 (2008): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

An experiment was conducted to determine the effect of undegraded protein and energy level on intake and digestibility of nutrient and glucose and urea blood content in dairy cows. The benefit of the research was to inform about the utilization of undegraded protein and energy level to optimize nutrient utilization in dairy cattle. The experiment was conducted in 4 month in the Department of Animal Nutrition and Feed Science, Faculty of Animal Science Gadjah Mada University. The experiment used 4 female rumen fistulated dry cows (Friesian Holstein Crossbreed) of 3.0-3.5 years old and 350–400 kg body weight. The treatments of this experiment were T1: 20% of undegraded protein (UDP) and 70% energy from requirement; T2: 20% undegraded protein and 120% energy from requirement; T3: 30% undegraded protein and 70% energy from requirement; and T4: 30% undegraded protein and 120% energy from requirement with Latin square design.  Variables observed were intake and digestibility of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), Acid detergent fiber (ADF),  and glucose and urea blood content. Collected data were analyzed by analysis of variances, and further differences were tested by orthogonal contrast. Results of the research showed that the level of UDP in the rations did not affect DM, OM, CP intake, rumen degradable protein (RDP), NDF and ADF; however level of energy had significant affect on DM, OM, CP,  RDP and ADF intake. The level of UDP resulted in non significant difference in DM; but significantly different on CP digestibility. Digestibility of DM and OM were significantly influenced by the level of energy, but it did not influence NDF and ADF digestibility. Cattle treated with the high energy rations had higher blood glucose concentration than cattle treated with the low energy rations. Blood urea in cattle fed low UDP rations was higher than cattle fed high UDP rations. (Animal Production 10(2): 96-101 (2008) Key Words: Undegraded protein, nitrogen, energy, blood metabolite
Ruminant Nutrition: The Role of Agriculture by Products in Beef Cattle Production Agus, Ali
Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY PROCEEDING INTERNATIONAL SEMINAR
Publisher : Proceeding INTERNATIONAL SEMINAR IMPROVING TROPICAL ANIMAL PRODUCTION FOR FOOD SECURITY

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Abstract

Increasing demand on food of animal origin including red meat in developing country is predicted doubled or even three fold in 2050, while the production especially that from ruminant legs well behind the projected human requirements. Feeding and nutrition have been reported to be the main constraint to ruminant production. In Indonesia, such as in other tropical countries, availability of feedstuffs is fluctuating during the year. Discussion on the available feeds is urgent in the face of rapidly depleting natural resources, climate change, urbanization, population pressure, decreasing land for crop production, and the increasing competition of arable land for food, feed and biofuel production. The efficiency of use of the available feed resources is very important and determinant for animal performance and productivity. The utilization of low quality feed mainly from crop residues as well as agricultural by products and other non-conventional feed resources has a common practiced. However, the different technologies are needed to maintain feed availability, improve feed quality and to optimize the nutrient utilization by the animal. This paper discuss briefly role of agricultural by products in beef cattle production. One may explore more comprehensive information concerned in this issue by accessing the papers and previous review papers as indicated in the references listed.   Key Words: Agricultural by Products, Nutritive Quality, Beef Cattle
Pemanfaatan Susu Bubuk Kadaluwarsa dalam Complete Calf Starter dan Pengaruhnya terhadap Konsentrasi VFA dan Gula Sapih Mukodiningsih, Sri; Budhi, Subur Priyono Sasmito; Agus, Ali; Astuti, A
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 4 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Peningkatan produksi susu dapat dilakukan melalui program cow replacement dengan pemeliharaan pedet yang optimal sebelum disapih. Hal ini dapat dicapai dengan pemberian pakan starter (calf starter dan sumber serat) bersama susu atau susu pengganti setelah lahir untuk mempercepat perkembangan rumen. Penelitian ini dilakukan dengan tujuan mengkaji penggunaan susu bubuk kedaluwarsa sebagai binder dalam complete calf starter (CCS) dan pengaruhnya terhadap perkembangan retikulo-rumen pedet pra sapih. Materi yang digunakan jerami jagung, jagung kuning, bungkil kedelai, dedak halus, premix dan pedet PFH pra sapih umur 1-2 minggu, bobot badan ±32 kg. Rancangan penelitian yang digunakan adalah rancangan acak lengkap terdiri dari 3 perlakuan. Perlakuan: I. Pellet 100% CCS (tanpa binder) (P0); II : Pellet 90% CCS + 10% susu bubuk kedaluwarsa (P10) ; III: Pellet 80% CCS + 20% susu bubuk kedaluwarsa (P20). Variabel yang diamati adalah kualitas kimia, fisik organoleptik (hardnees dan durability)  dan kualitas biologis (konsumsi, pertambahan bobot badan harian (pbbh), kadar gula dan VFA darah).  Data diolah dengan analisis variansi dilanjutkan dengan uji ganda Duncan. Uji biologis pada pedet umur 3 minggu, menghasilkan konsumsi, pbbh, konsentrasi gula dan VFA darah masih dalam kisaran normal.  Disimpulkan bahwa penggunaan susu bubuk kadaluwarsa hingga 20% dalam CCS dapat diberikan pada pedet PFH umur 2 minggu dan menghasilkan  indicator perkembangan rumen pedet yang baik
The Exploration of Bioactive Peptides that Docked to SARS-CoV-2 Spike Protein from Goats’ Milk Beta-Casein by In Silico Widodo, Hermawan Setyo; Murti, Tridjoko Wisnu; Agus, Ali; Pertiwiningrum, Ambar
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.7297

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Beta-casein in milk is known to be a bioactive peptide producer because of its amino acid sequence. Bioactive peptides have prospected molecules that can adhere with SARS-CoV-2 spike protein, so they can inhibit the virus from hooking up with human cell receptor protein. The research is aimed to find any peptides from goat’s milk beta-casein that are prospective candidates as SARS-CoV-2 spike protein inhibitors. Goat’s milk beta-casein was simulated as being digested by the digestive tract. Pepsin, trypsin, and chymotrypsin enzymes cut the beta-casein amino acids sequence into small peptides. Then, their bioavailability was predicted by Lipinski’s Rules of 5 (Ro5), any most fitted peptides to the rules will be simulated to dock to SARS-CoV2 spike protein besides Curcumin as the control ligand. Peptides with the best bind activity with the spike protein will be selected as inhibitor candidates. Peptide QPK is selected as a SARS-CoV-2 inhibitor candidate because it has better affinity energy than Curcumin or other selected peptides.
Pemanfaatan Digital Marketing dan Peningkatan Motivasi Jiwa Pengusaha dalam Mengembangkan Desa Wisata di Desa Karangsari Garut Agus, Ali; Muratin, Muratin; Ardista, Rini; Kusuma, Aditya Putra; Parantoro, Apri; Freddy, Daulat; Priyatno, Suratman Hadi
Jurnal Pengabdian Dharma Laksana Vol. 6 No. 1 (2023): JPDL (Jurnal Pengabdian Dharma Laksana)
Publisher : LPPM Universitas Pamulang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32493/j.pdl.v6i1.26330

Abstract

Indonesia menargetkan pendapatan dari sector pariwisata meningkat hingga 1,7 miliar dolar AS atau sekitar Rp24 miliar di 2023 mendatang. Begitu juga kontribusi Product Domestic Bruto (PDB) pariwisata ditarget meningkat sebesar 4,3 persen.  Pariwisata juga tumbuh secara spektakuler menjadi sektor andalan dalam pendapatan ekonomi masyarakat.  Desa Karangsari Kecamatan Pakenjeng Kabupaten Garut yang memiliki tempat wisata pantai yang menarik,bersih, elok, tentram, aman dan humanis. Kita dapat melihat sunrise di pagi hari dan sunset di sore hari .  Hal ini menjadi daya tarik tersendiri bagi para wisatawan baik lokal maupun non local sehingga banyak dikunjungi oleh wisatawan . Namun sangat disayangkan objek wisata tersebut belum optimal. Jumlah wistawan yang datang masih sangat terbatas.  Hal tersebut disebabkan kurangnya informasi dan promosi tentang Desa Wisata tersebut.  Salah satu promosi yang dapat diandalakn sekarang ini adalah media social yang ada di internet.  Promosi tersebut akan  memberikan peluang yang besar untuk dapat mempromosikan dan menginformasikan adanya desa wisata pantai.  Melalui media maya atau digital media internet maka wisata pantai tersebut di kenal di seluruh dunia, dengan demikian maka meskipun letak pantainya berada di pedesaan yang cukup terpencil, Desa Karangsari,  tetap banyak diketahui dan diminati oleh orang banyak.  Tujuan kegiatan pengabdian kepada masyarakat ini adalah memberikan pemahaman tentang digital marketing dalam mempromosikan wisata pantai laut selatan yang berada di desa Karangsari Kecamatan Pakenjeng Kabupaten Garut.