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Isolation and Analysis of Bioactive Compounds Endophytic Bacteria of Sea Fern (Acrtostichum aureum L.) from Bengkalis Island, Riau Tetty Marta Linda; Azizul Berlyansah; Bernadeta Leni Fibriarti; Nery Sofiyanti; Silvera Devi
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3104

Abstract

Endophytic bacteria have been known to produce high levels of secondary metabolites. The sterile leaves of the sea fern (Acrostichum aureum L.) contain many phytochemical compounds. This sea fern is often found in peat swamp areas. The aim of this study was to isolate, partial characterization, and analysis of bioactive compounds from endophytic bacteria of sea ferns to determine their bioactive compounds. Endophytic bacteria were isolated by a direct technique using nutrient agar (NA) media. In this study, the endophytic bacteria were characterized by macroscopic, microscopic, and Gram. Several tests were further performed, namely phytochemical test and Fourier Transform Infra Red (FT IR) FTIR tests from a crude extract of secondary metabolites of bacteria. The results of the isolation obtained six isolates of endophytic bacteria consisting of all isolates were Gram-negative. The phytochemical test results for the six isolates were positive for alkaloids and saponins but no steroids. Based on the results of phytochemical tests and FT IR, it is suspected that the isolate DSB 1.6 has similarities with the alkaloid functional group. This endophytic bacterial isolate can be developed as a candidate for produced of pharmacological compounds.
Standarisasi produk dan strategi digital marketing untuk meningkatkan pemasaran pada UMKM VCO Bertuah Sujarwati Sujarwati; Siti Fatonah; Bernadeta Leni Fibriarti; Silvera Devi Sy; Oswati Hasanah; Arini Arini
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.234-240

Abstract

VCO Bertuah is an MSME in Pekanbaru that produces virgin coconut oil. Bertuah VCO has legality permits in the form of IUMK, NIB and P-IRT Certificate. However, Bertuah VCO does not have a halal certificate, BPOM distribution permit, and product SNI. The marketing of Bertuah VCO products is mostly done online, especially through social media platforms such as Facebook and Instagram. Other digital marketing strategies such as youtube and websites have not been carried out. Based on the results of the activity, it can be concluded that UMKM need assistance to take care of business legality. It is necessary to have active communication between UMKM and government agencies in charge of business licensing. Assistance activities have succeeded in increasing partners' understanding of the business legality management procedures. Assistance activities are very helpful for partners in preparing documents for business legality requirements. Assistance activities increase partners' understanding of digital marketing strategies through the website, instagram and youtube. Assistance activities help partners prepare advertising content in the form of product photos, instagram feed designs, video testimonials, and good ad copywriting.
Optimization of Nitrogen and Mineral Sources in Liquid Media for Amylase Production Thermophilic Aspergillus sp. LBKURCC304 Mhd Muslim Syaifullah; Devi, Silvera; Saryono, Saryono; Itnawita, Itnawita; Mukhlis, Mukhlis
Photon: Journal of Natural Sciences and Technology Vol 14 No 1 (2023): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v14i1.5468

Abstract

The application of amylase is relatively large, used in various industries: textiles, pulp and paper, feed, detergents, and food. Therefore, the isolation and optimization of amylase production from various microorganisms has been ongoing. Optimization of amylase production from Aspergillus sp. LBKURCC304 was carried out in liquid media with variable sources of nitrogen (soy flour, tempeh flour or catfish flour) and minerals (FeSO47H2O; CaCl2 2H2O; MgSO4 7H2O; MnSO4 H2O, BaCl2 2H2O) incubated at 50⁰C for 11 days with an agitation speed of 150 rpm. The amylase activity produced was determined by the Nelson-Semogyi method, the protein content by the Lowry method and the specific activity was calculated from the ratio of the amylase activity to the protein content. The research data were statistically tested using ANOVA and the multiple-distance Duncan method at a significance level of 5%. The results of the study showed that the highest amylase activity of 0.0084±0.0014 U/mL was produced in a medium with a nitrogen source of tempeh, the mineral was MgSO4.7H2O, a concentration of 0.05%. The protein content was 0.5111±0.0073 mg/ml and the specific activity was 0.0164±0.00 U/mg.
Isolation and antimicrobial activity of lactic acid bacteria from the traditional fermented food cangkuok semaung in Riau, Indonesia Pratiwi, Nova Wahyu; Saryono, Saryono; Ismawati, Ismawati; Devi, Silvera; Suraya, Nabella; Lovenia, Nia; Nurafriza, Widya; Sasmita, Ellen Rosyelina; Ardhi, Aulia
Jurnal Ilmiah Pertanian Vol. 22 No. 2 (2025): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v22i2.27537

Abstract

Fermentation can be an effective step in addressing postharvest handling challenges and enhancing nutritional value through the conversion of perishable crops into more stable products. One of the traditional fermented foods originating from Kuantan Singingi Regency, Riau Province, Indonesia, is cangkuok semaung (CS). This food is prepared by fermenting all fruit components of the semaung (Pangium edule). This research aimed to isolate and identify lactic acid bacteria (LAB) from CS and investigate their antimicrobial properties. The bacteria were isolated from the food, purified using the quadrant streak method on the De Man–Rogosa–Sharpe agar, and continued with morphological analysis and antimicrobial activities evaluation. The findings of this study revealed that 45 isolates were gram-positive bacteria identified as Lactobacillus sp. Among the LAB isolates, the highest antimicrobial activity against Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae were shown by isolates of S12, S14, S27, and S24, respectively. These results suggest that LAB from CS, especially isolates S12 and S14, have promising antimicrobial properties and show potential as probiotic candidates that merit further investigation into possible applications in food and health sectors.
Optimization of Contact Time and Stirring Speed in The Adsorption of Cd(II) Using Acacia crassicarpa Bark Powder as Adsorbent Mukhlis; Hidayah, Nurul; Hanifah, Abu; Itnawita; Anita, Sofia; Devi, Silvera; Tamboesai, Emrizal Mahidin
Indonesian Journal of Chemical Analysis (IJCA) Vol. 8 No. 2 (2025): Indonesian Journal of Chemical Analysis
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/ijca.vol8.iss2.art2

Abstract

Acacia crassicarpa bark powder showed potential as an adsorbent due to its high cellulose content of approximately 50%. This research aimed to determine the optimum contact time, stirring speed, and adsorption kinetic models of Acacia crassicarpa bark powder for Cd(II) removal. Adsorbent characterization was conducted using a Surface Area and Pore Size Analyzer, FTIR, and SEM-EDX, while batch adsorption experiments were conducted to evaluate performance. The adsorbent exhibited a specific surface area of 0.460 m²/g and pore diameters ranging from 32.707 to 45.426 Å, indicating mesoporous characteristics. FTIR analysis identified functional groups such as O–H, C=O, and O–Cd. SEM-EDX analysis before adsorption revealed a rough surface with open, irregular pores and dominant elements including C, N, and O. After adsorption, the surface appeared smoother, with pores filled by Cd(II), as confirmed by Cd peaks in the EDX spectrum. Optimum conditions were obtained at a contact time of 70 minutes and a stirring speed of 120 rpm, resulting in an adsorption efficiency of 96.93% and a 1.3613 mg/g capacity. The adsorption kinetics followed a pseudo-second-order model (R2 = 0.9985), indicating that the adsorption mechanism occurred via chemisorption.