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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI AL KAUNIYAH Warta IHP (Warta Industri Hasil Pertanian) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Chimica et Natura Acta Menara Perkebunan Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Bulletin of Chemical Reaction Engineering & Catalysis Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Biodiversitas Journal of Biological Diversity
Sumi Hudiyono
Department Of Chemistry, Faculty Of Mathematics And Natural Science, Universitas Indonesia, Kampus UI Depok, Depok, 16424, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Lira Windriawati Listriyani; Sumi Hudiyono; Rudiyono Rudiyono; Siti Zulaeha; Ahmad Wibisana
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.
Sintesis reagen imunokimia untuk deteksi okratoksin dengan metode imunokromatografik nanopartikel emas Synthesis of immunochemical reagent for ochratoxin detection using gold nanoparticle immunochromatographic method Irma KRESNAWATY; Romsyah MARYAM; . SUHARYANTO; Sumi HUDIYONO
Menara Perkebunan Vol. 83 No. 1: 83 (1), 2015
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v83i1.8

Abstract

Abstract    The quality of Indonesiam coffee andcocoa products has been declined due to the contamination of fungi producing ochratoxin, a serious human mycotoxin. Therefore, develop-ment of fast, accurate and simple method for early detection of ochratoxin contamination is required. As a part of research attempted to develop early detection technique of ochratoxin in severall commodities, especially agricultural products, the objective of this study was to produce immunochemical reagent for ochratoxin detectionusing immunoglobulin Y (IgY) based on immunochromatographic method. Results showed that OTA-OVA could be synthesized using active ester method with addition of N-hydroxy-succiimide and dicyclocarboimide. The inter-mediate compound produced showed C=O stretching vibrational band at 1600 cm-1 and C-O stretching vibrational band at 1300-1000 cm-1. Antibody-gold nanoparticle conjugate was optimally produced at pH 9 and with antibody dilution of 1:7.5 (v/v). There was 50 nm absorb-tion shift in visible absorbtion after the antibody was conjugated with gold nanoparticle. Even though the test strip did not show  clear visual-ization, the cut off of ochratoxin concentrationis obviously determined at 10 ppb. This results suggest thatthe technique could be used to detect ochratoxin contamination. Abstrak Komoditas kopi dan kakao Indonesia ter-kendala masalah mutu produk yang rendah akibat kontaminasi jamur yang menghasilkan okra-toksin. Okratoksin merupakan mikotoksin yang membahayakan kesehatan manusia. Oleh karena itu, cara deteksi dini kontaminasi okratoksin yang cepat, akurat dan mudah perlu dikembangkan. Sebagai bagian dari usaha untuk mengembangkan teknik deteksi dini okratoksin pada pada berbagai komoditas, khususnya produk pertanian, pene-litian ini bertujuan menghasilkan reagen imuno-kimia yang menggunakan antibodi immune-globulin Y (IgY) berdasarkan metode imunokro-matografik untuk deteksi okratoksin. Hasil penelitian menunjukkan bahwa OTA-OVA dapat disintesis dengan metode ester aktif dengan menambahkan N-hidroksisuksiimida dan disiklo-karboimida. Senyawa antara yang dihasilkan memiliki absorpsi pada frekuensi 1600 cm-1 yang menunjukkan adanya vibrasi ulur ikatan C=O dan adanya banyak absorpsi pada 1300-1000 cm-1 yang mengindikasikan adanya serapan ulur yang kuat ikatan C-O. Konjugat antibodi-nanopartikel emas dihasilkan optimum pada pH 9 dan dengan pengenceran antibodi 1:7,5 (v/v). Hasil pengujian spektrofotometer visible menunjukkan adanya pergeseran serapan sebesar 50 nm setelah anti-bodi dikonjugasikan pada nanopartikel emas. Meskipun secara visual tidak begitu jelas, tetapi hasil pengujian pada teststrip menunjukkan bahwa nilai cut off konsentrasi okratoksin yang terdeteksi adalah10 ppb. Hasil ini menunjukkan bahwa teknik yang dikembangkan dapat diguna-kan untuk deteksi kontaminasi okratoksin.
Produksi imunoglobulin Y (IgY) untuk pengembangan metode deteksi dini kontaminasi okratoksin (Immunoglobulin Y (IgY) production to develop an early detection method for ochratoxin contamination) Irma KRESNAWATY; . SUHARYANTO; . SISWANTO; Sumi HUDIYONO
Menara Perkebunan Vol. 86 No. 1 (2018): 86 (1), 2018
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v1i1.279

Abstract

Indonesian coffee and cocoa commodities are constrained by low product quality problem due to contamination of fungal metabolites which  producing ochratoxin A (OTA). Ochratoxin is neprotoxic, immunogenic, carcinogenic and teratogenic to the human health. Early detection method on site detection should be developed  because of  those negative effects. The aim of this study  was to produce antibody to develop a method for  OTA detection. Antibody was produced by immunization of egg laying hen. Antibody-produced was sepatared and analyzed using ELISA (Enzyme-Linked Immunosorbent Assay) and DBIA (dot blot immunoassay),and tested its composition using HPLC and SDS PAGE. The results showed that anti-OTA polyclonal antibodies had been obtained already from chicken eggs in the 4th period (7 weeks after initial immunization). These antibodies showed anti-OTA reactivity by DBIA method and still showed anti-OTA reactivity up to 9th period (12 weeks after initial immunization). The anti-BSA antibodies produced should be removed to increase the sensitivity of antibodies againts ochratoxin A. The separation of BSA antibodies can be conducted by the absorption of the protein.  [Keywords: ochratoxin A; early detection; antibody IgY]. AbstrakKomoditas kopi dan kakao Indonesia terkendala masalah mutu produk yang rendah akibat kontaminasi cendawan penghasil okratoksin A. Okratoksin A (OTA) bersifat neprotoksik, imunogenik, karsinogenik dan teratogenik yang membahayakan kesehatan manusia. Karena efek negatif yang diakibatkan oleh mikotoksin ini, maka perlu dikembangkan deteksi dini kontaminasi okratoksin langsung di lokasi. Penelitian ini bertujuan menghasilkan antibodi imunoglobulin Y (IgY) untuk mengembangkan metode perakitan perangkat deteksi cepat berbasis imunologi untuk deteksi OTA. Antibodi dihasilkan menggunakan uji ayam petelur. Antibodi yang dihasilkan dipisahkan dan dianalisis aktivitasnya dengan ELISA (Enzyme-Linked Immunosorbent Assay) dan DBIA (dot blot immunoassay), serta diuji komposisinya dengan HPLC dan SDS PAGE. Hasil penelitian menunjukkan bahwa antibodi poliklonal anti-OTA sudah diperoleh dari telur ayam pada periode ke-4 (7 minggu setelah imunisasi awal). Antibodi ini menunjukkan reaktivitas anti-OTA dengan metode DBIA dan masih menunjukkan reaktivitas anti-OTA sampai periode 9 (12 minggu setelah imunisasi awal). Komposisi asam amino antibodi anti-OTA menunjukkan perbedaan dengan komposisi asam amino IgY di database. Antibodi anti BSA yang dihasilkan harus dihilangkan terlebih dahulu untuk meningkatkan sensitivitas antibodi terhadap okratoksin A dan pemisahan dapat dilakukan dengan penyerapan antibodi BSA.[Kata Kunci:  okratoksin A;  deteksi dini; antibodi IgY].
Gibberellic acid (GA) role in acetyl-coA carboxylase enzyme regulation and in improving oil palm yield Irma Kresnawaty; Djoko Santoso; Galuh Wening Permatasari; Sumi Hudiyono
Menara Perkebunan Vol. 91 No. 2 (2023): 91 (2), 2023
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v91i2.533

Abstract

Seaweed specifically, Sargassum sp. is known to contains a boosting hormone growth that has been promoted plant growth and yield due to the containing of auxin, gibberelic acid (GA) and cytokinine, and also some amino acids especially glutamic acid. Those composition could be used as an booster of palm oil production which related to acetyl co-A carboxylase activity (ACC). ACC is the rate determination step in fatty acid accumulation, and becomes active through dephosphorylation of some serine residues that induced by magnesium and glutamate. Moreover, ACC was regulated by AtWRI1 and AtWRI1-TCP4 interaction, a mechanism that allow fine-tuning of the oil biosynthetic pathway. In this research we conducted gene expression experiments, and molecular docking analyses to study the possible mechanism of seaweed composition  stimulating oil  accumulation  in the    oil palm. Further  analysis  was  conducted  to  ensure whether the interaction between TCP4 and candidate inhibitors were able to phosphorylate TCP4 and decrease its activity. GA application resulted in the increase of oil accumulation in 1 month after application, although in the second month the oil accumulation showed decreasing. Increase of oil accumulation in the first month in line with the increase of the expression of ACC in 3rd and 5th weeks. Meanwhile, TCP4 showed decrease expression that resulted in the increase of the WRI1 in 5th week. From this result, it was indicated that GA application could block the TCP4, so it could not interact with WRI1, resulted in the expression of WRI1 and ACC. This interaction stimulates the oil accumulation in oil palm.  
OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Listriyani, Lira Windriawati; Hudiyono, Sumi; Rudiyono, Rudiyono; Zulaeha, Siti; Wibisana, Ahmad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1748

Abstract

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.
Effect of Gamma Irradiation on Microbial Quality and Physicochemical Attributes of Tilapia (Oreochromis niloticus) Indra Mustika Pratama; Ashri Mukti Benita; Aldi Mujiyanto; Bani Fadhilah Akbar; Sumi Hudiyono; Irawan Sugoro
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i2.46518

Abstract

Tilapia (Oreochromis niloticus) is a widely consumed freshwater fish commodity that is highly susceptible to microbial spoilage, leading to rapid quality deterioration and a short shelf life. Gamma irradiation is a promising preservation technology to address this issue. This study aimed to evaluate the effectiveness of gamma irradiation in reducing microbial contamination and maintaining the physicochemical quality of fresh tilapia. Samples were irradiated at a dose of 3 kGy (dose rate 1 kGy/h) in combination with vacuum packaging and refrigeration. Microbiological analyses included total plate count, Salmonella–Shigella, and total mold and yeast, while physicochemical parameters comprised pH, ash, fat, protein, water activity (aw), total volatile base nitrogen (TVB-N), trimethylamine (TMA-N), and color (L*, a*, b*), using non-irradiated samples as controls. The results showed that irradiation reduced total bacteria and Salmonella–Shigella by approximately 2 log cycles and eliminated molds and yeasts without significant changes (P >0.05) in physicochemical parameters compared to control samples. These findings indicate that gamma irradiation at 3 kGy is effective in improving microbial safety while preserving the physicochemical quality of fresh tilapia.
Effects of plant growth regulator from seaweed extracts on expression of genes regulating the oil and related metabolites productions in oil palm IRMA KRESNAWATY; DJOKO SANTOSO; SUMI HUDIYONO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 2 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230225

Abstract

Abstract. Kresnawaty I, Santoso D,Hudiyono S. 2022. Effects of plant growth regulator from seaweed extracts on expression of genes regulating the oil and related metabolites productions in oil palm. Biodiversitas 23: 793-800. Plant growth regulators (PGRs) have been used in agricultural practicesto regulate various processes, such as germination, vegetative growth, reproduction, maturation, leaf senescence and post-harvest. Seaweed extract as natural PGRs source containsauxin, cytokines, and gibberellins. Although PGRs have been used for decades, the effects of these compounds on the production of secondary metabolites, proteins and related gene-expressionare not yetwell knownin some perennial crops.To study those,treatments consist of trunk injection of oil palm trees with biostimulant that contain PGRs from seaweed extract. Effects of treatments compared were biostimulant (B), biostimulant with activator for oil accumulation (BA) and control (C). The results indicated that the biostimulant treatments affected ACCase Biotin Carboxylase and HMG-CoA genes expression ratio andwere maximum at4 weeks after treatments (WAT) for biostimulant with activators treatments and 8 WAT for biostimulants without activator. Effect of biostimulants treatments in inducing the gene expression was not significant after 8 WAT. Moreover samples with addition biostimulant in 1 and 7 had the highest values ??ofthe gibberellin (GID) gene expression. Oil accumulation showed increase in 4 WAT dan had optimum level in 8 WATbecause of activators addition, especially increased of palmitic and linoleic acid.
Anticandidal and antioxidant potencies of endophytic fungi associated with Tinospora crispa AHMAD FATHONI; SUMI HUDIYONO; ANTONIUS HERRY CAHYANA; MUHAMMAD ILYAS; ISMU PURNANINGSIH; ANDRIA AGUSTA
Biodiversitas Journal of Biological Diversity Vol. 24 No. 5 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240506

Abstract

Abstract. Fathoni A, Hudiyono S, Cahyana AH, Ilyas M, Purnaningsih I, Agusta A. 2023. Anticandidal and antioxidant potencies of endophytic fungi associated with Tinospora crispa. Biodiversitas 24: 2547-2555. Natural products including endophytic fungi, have opportunities for medicinal uses as anticandidal and antioxidant agents. This study aimed to investigate the anticandidal and antioxidant activities of endophytic fungi isolated from Brotowali (Tinospora crispa (L.) Miers ex Hook.fil. & Thomson) using disc diffusion, thin layer chromatography (TLC) dot-blot, and microdilution methods. Eighty extracts were screened for anticandidal activity against Candida albicans (C.P.Robin) Berkhout and antioxidants against 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. Disc diffusion results showed that 38 endophytic fungi had an anticandidal activity with an inhibition zone diameter (IZD) of 7.00±00 to 18.1± 0.29 mm and 4 extracts with minimum inhibitory concentration (MIC) values of 32-64 µg/mL (strong, MIC<100). The TLC dot-blot results showed that 23 extracts had antioxidant activity and 8 extracts were very strong with an inhibitory concentration of 50% (IC50) values of 7.76±0.77 to 25.54±1.37 µg/mL, while the antioxidant activity index (AAI) values ranged from 2.01±0.10 to 6.64±0.63 (very strong, AAI>2). Results of the Pearson correlations coefficient between the total phenolic content (TPC) value and AAI value, revealed that there was a low correlation, while the correlation of IZD and MIC as anticandidal has a moderate negative (the r value 0.332, and -0.673, respectively (P<0.01). Purification of bioactive compounds is necessary, and endophytic fungi associated with T. crispa can serve as a potential source of natural antioxidants and anticandidal agents.
Citronellyl Caffeate as a Novel α-Glucosidase Inhibitor with Potential Antidiabetic Activity Lestari Elvina Wibowo; Sumi Hudiyono; Muhammad Hanafi; Nina Artanti
Molekul Vol 21 No 2 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.2.18162

Abstract

ABSTRACT. Caffeic acid, a well-studied phenolic acid, has been extensively investigated for its diverse bioactivities, including antidiabetic, anti-inflammatory, antioxidant, antimicrobial, and antiviral properties. Esterification of caffeic acid has been explored to modulate its physicochemical and biological properties, however, optimization of lipophilicity while maintaining inhibitory activity remains a challenge. In this context, citronellyl caffeate was designed as a structurally optimized derivative that enhances α-glucosidase inhibition while improving lipophilicity and permeability. To enhance antidiabetic bioactivity through α-glucosidase inhibition, caffeic acid and citronellol were coupled using dicyclohexylcarbodiimide (DCC) as an activator and 4-dimethylaminopyridine (DMAP) as a catalyst with dimethylformamide (DMF) as the solvent. The synthesis was carried out under reflux conditions, followed by purification via liquid–liquid extraction and column chromatography, with thin-layer chromatography (TLC) used to confirm purity. The resulting compound, citronellyl caffeate, was characterized using ¹H NMR and ¹³C NMR spectroscopy. Evaluation through α-glucosidase inhibition assays demonstrated that citronellyl caffeate showed enhanced α-glucosidase inhibitory activity (IC₅₀ = 31.17 µg/mL) compared to its precursors, caffeic acid and citronellol, despite being less potent than quercetin (IC₅₀ = 2.43 µg/mL), it demonstrates biologically relevant inhibition consistent with successful structural optimization. Keywords: antidiabetic, caffeic acid, citronellol, glucosidase, synthesis
Co-Authors -, Budiawan -, Yuniarti . Rudiyono . SISWANTO AHMAD FATHONI Ahmad Wibisana AHMAD WIBISANA Ahmad Wibisana, Ahmad Aldi Mujiyanto ANDRIA AGUSTA ANTONIUS HERRY CAHYANA Ashri Mukti Benita Awaliatul Barkah Bani Fadhilah Akbar Barkah, Awaliatul Budiawan - Chiko Harahap DJOKO SANTOSO Djoko Santoso Galuh Wening Permatasari Handayani, Sri Hanny Hafiar Heny Suseno Ika Novianingsih Indra Mustika Pratama Irawan Sugoro IRMA KRESNAWATY Irma Kresnawaty Irma KRESNAWATY Irma Kresnawaty ISMU PURNANINGSIH Lestari Elvina Wibowo Lira Windriawati Listriyani Lira Windriawati Listriyani Listriyani, Lira Windriawati MUHAMMAD ILYAS Muhammad Hanafi Muslim Muslim Nina Artanti Novianingsih, Ika Partomuan Simanjuntak Romsyah Maryam Rosalina, Khoerunissa Novianti Rudiyono Rudiyono Rudiyono, Rudiyono Siti Zulaeha Siti Zulaeha Siti Zulaeha, Siti Sri Handayani Sri Handayani Sri Handayani Suharyanto Tuti Wukirsari Wien Kusharyoto Yatri Hapsari Yuniarti -