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All Journal Pharmaciana: Jurnal Kefarmasian Althea Medical Journal The Journal of The Indonesian Medical Students´ Association Damianus Journal of Medicine Indonesian Journal of Tropical and Infectious Disease Universa Medicina Dinamisia: Jurnal Pengabdian Kepada Masyarakat Journal of Community Service and Empowerment JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Mitramas Jurnal Pengabdian dan Pemberdayaan Masyarakat Mitramas Jurnal Pengabdian dan Pemberdayaan Masyarakat
Sem Samuel Surja
Department Of Parasitology, Faculty Of Medicine And Health Sciences, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Althea Medical Journal

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Preservation in Paraffin Oil: Alternative for Fungi Preservation in Simple Laboratory Concept Sem Samuel Surja; Gabi Vania Sally; Favian Sergius Ruby Hartoyo; Sandy Vitria Kurniawan; Yanto Budiman
Althea Medical Journal Vol 7, No 3 (2020)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v7n3.1975

Abstract

Background: A good collection of fungi culture can be maintained with good preservation methods. With appropriate methods, preservation can be performed in low-budget laboratories, such as with periodic subculture and preservation in either sterile water or in paraffin oil. The aim of this study was to assess the viability, morphology, contamination, and antifungal susceptibility profile of fungi preserved in sterile water or paraffin oil compared to a periodic subculture.Methods: This study was an experimental study using Aspergillus sp., Trichophyton mentagrophytes and Candida albicans. The fungi were preserved by a periodic subculture or in sterile water or in paraffin oil for six months. A comparison of viability, morphology, contamination, and antifungal susceptibility profile between methods were made before and after six months of study.Results: The viability of all the fungi was maintained in both periodic subculture and preservation with paraffin oil. However, the T. mentagrophytes was not viable in preservation using sterile water. All methods could maintain the morphology without contamination. There were reductions in the inhibition zone against antifungal drug in preservation with sterile water and paraffin oil.Conclusion: Preservation in paraffin oil is superior to preservation in sterile water in terms of maintaining fungal viability. This method is suitable for the use in a simple laboratory, albeit considerations should be given to the reduction of inhibition zone.
Anticandidal Activity of Lactoferrin, Apolactoferrin, and Oligosaccharides on Mueller-Hinton and Sabouraud Dextrose Agar against Fluconazole Resistant-Candida Albicans Maureen Miracle Stella; Sem Samuel Surja; Zita Arieselia
Althea Medical Journal Vol 9, No 3 (2022)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v9n3.2408

Abstract

Background: The demand for novel, useful, potential, and safe antifungal drugs and rapid fungal susceptibility test methods due to antifungal resistance and high prevalence of Candida albicans infection are continuing. Therefore, this study aimed to assess and compare the antifungal activity of lactoferrin, apolactoferrin, and oligosaccharides isolated from human, bovine, goat, and formula milk against C. albicans on Mueller-Hinton agar supplemented with 2% glucose and 5 µg/mL methylene blue and sabouraud dextrose agar.Methods: Lactoferrin, apolactoferrin, and oligosaccharides were extracted from human, bovine, goat, and formula milk. Lactoferrin was identified using the Bradford test and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis. Oligosaccharides were identified using Thin-Layer Chromatography. The antifungal activity of lactoferrin, apolactoferrin, and oligosaccharides against fluconazole-resistant Candida albicans ATCC 10231 was determined and compared using the disk diffusion method on Mueller-Hinton agar and sabouraud dextrose agar. The inhibition zone formed around the disk was observed after 24 hours of incubation.Results: Lactoferrin showed an inhibition zone on sabouraud dextrose agar against C. albicans, but not on Mueller-Hinton agar. Meanwhile, apolactoferrin and oligosaccharides showed no antifungal activity on both agar media.Conclusions: Different agar media in the diffusion disk test can give different results even though using the same test method and substance. These results could shed light and become the useful references on why some potential antifungals could yield a different results in in-vitro studies, in-vivo studies, or clinical trials.
Co-Authors Arifin, Erlangga Saputra Favian Sergius Ruby Hartoyo Felicia Anggraini, Felicia Gabi Vania Sally Gabi Vania Sally Handinata Indrawan Hanna Yolanda Hendrawan Ariwibowo Hengestu, Anastasia Hermawan, Melyawati Jessica Fedriani Joprang, Freggy Spicano Kaisar, Maria Mardalena Martini Kristin, Helen Kurniawan, Sandy Vitria Lorettha Wijaya Margareta, Revi Maureen Miracle Stella Muhyidin, Muhammad Fadli Olivia, Vera Pramanta, Pramanta Prasetya, Alver Rachel, Clarissa Rita Dewi Ruby, Rivaldi Setiawan, Glory Laura Soegianto Ali Stefani Krisanti Toemon, Agnes Immanuela Widowati, Tria Asri Wijaya, Meiliyana Yanto Budiman Zita Arieselia