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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Marine Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Marine Research in Indonesia Indonesian Journal of Natural Pigments Teknologi Indonesia Life Science Makara Journal of Technology Annales Bogorienses
DWI SUSILANINGSIH
Research Centre for Biotechnology, Indonesian Institute of Sciences, Cibinong - Bogor, 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Other

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Economically Potential Pigments from Marine Blue-Green Algae for the Application in Food and Health Salim, Katarina Purnomo; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Susilaningsih, Dwi; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.37

Abstract

Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study focused on the identification of the composition of the dominant chlorophylls and carotenoids. The results showed the presence of Zeaxanthin, Chlorophyll a, and β-carotene. The economic potency of those dominant pigments will be reviewed in this article.
Pengaruh Substitusi Limbah Cair Tahu Untuk Menstimulasi Pembentukan Lipida Pada Chlorella sp. Harahap, Puspita Sari; Susanto, Antonius Budi; Susilaningsih, Dwi; Rahma, Delicia Yunita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.672 KB) | DOI: 10.14710/jmr.v2i1.2070

Abstract

Chlorella sp. is one of microalgae which has potential lipid content. Chlorella sp. has a rapid growth rate and also easy to maintain. Therefore, chlorella has potency as source in biodiesel production.This aim of study is to determine substitution of carbon source as regular medium with tofu liquid waste to enhanced lipid production of Chlorella sp. This research was conducted by laboratory experimental method using Completely Randomized Design (CRD) with six treatments (three replicates) and one control. Material in this research is Chlorella sp. that obtained from Puslit Bioteknologi- Bioproses division, Lembaga Ilmu Pengetahuan Indonesia, Cibinong. Research’s data is analyzed using analysis variant (for the normal and homogenous data) or using non-parametric (for the abnormal and in homogenous data). The result of this study indicates treatment which has higher lipid is Chlorella sp.’s cultivation with tofu liquid waste addition 15 % in day 42nd (0,5160 g/ L), and treatment with tofu liquid waste addition 20 % in day 45th has the highest oil content (0,4040 g/ L).Culture of Chlorella sp. with tofu liquid waste as medium resulted decrese lipid production.
Kandungan Lipid Beberapa Jenis Sianobakteria Laut Sebagai Bahan Sumber Penghasil Biodiesel Sobari, Rifana; Susanto, Antonius Budi; Susilaningsih, Dwi; Rahma, Delicia Yunita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.083 KB) | DOI: 10.14710/jmr.v2i1.2063

Abstract

Microalgae are an organism that contains of chlorophyll, so it can make the process of photosynthesis. The process of photosynthesis is the content of lipid producing microalgae as a potential feedstock biodiesel producer. Microalgae have the ability to generate huge natural oils (lipids). The aim of this study is to investigate the characteristic of morfologi and growth of microalgae marine cyanobacteria of Indonesian water that have the most lipid content, so it can be used as raw material for biodiesel. The research was carried out in September 2011 - February 2012. The implementation of the research carried out at the Laboratory of Bioprocess, Biotechnology Research Center Indonesian Institute of Sciences (LIPI) Cibinong, Bogor. Result microalga identification done using Nikon SE light microskop with identification book whareas lipid content ekstraction microalga done with Bligh and Dyer method (1959). The results showed that the microalgae sianobacteria had base of morfologi characteristic is the type of Oscillatoria sp.1, Oscillatoria sp.2, Nostoc sp., Synecococcus sp. and Lyngbya sp.. The highges growth OD marine cyanobacteria is type of Nostoc sp. and the lowest growth is type of Oscillatoria sp.2. Lipid content on Oscillatoria sp.1 4,2 mg/25 ml, Oscillatoria sp.2 3,7 mg/ 25 ml, Nostoc sp. 3,3 mg/25 ml, Synecococcus sp. 4 mg/25 ml and Lyngbya sp. 7,1 mg/25 ml.
IDENTIFICATION OF BIOACTIVE COMPOUND FROM MICROALGA BTM 11 AS HEPATITIS C VIRUS RNA HELICASE INHIBITOR Mustopa, Apon Zaenal; Umami, Rifqiyah Nur; Putri, Prabawati Hyunita; Susilaningsih, Dwi; Farida, Hilda
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2198

Abstract

ABSTRACTHepatitis C virus (HCV) is the major causative agent of chronic liver disease. Recently, the inhibition of NS3 RNA helicase/ATPase activity is being explored as the specifically targeted antiviral therapy (STAT) against HCV infection. This study was aimed to elucidate potential candidates for anti-HCV therapy derived from Indonesian indigenous microalgae. The microalga designated as BTM 11 was isolated and cultured. Methanol extract of BTM 11 was screened as the opponent of purified HCV NS3 RNA helicase enzyme through colorimetric ATPase assay. Screening of chemical compound and fractionation by using gel filtration chromatography with eluent of methanol : chloroform (1:99) were conducted for identification and isolation of the bioactive compounds. The third fraction of fractionated sample showed a relatively strong ATPase inhibitory effect (81.23 ± 2.25 %) compared to the negative control. Further analysis of third fraction using thin layer chromatography (TLC) with eluent of chloroform : methanol (9:2) gave two spots with the Rf value of 0.8 and 0.37, respectively. In addition, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 and 16.617 minutes which absorbed at 266 and 230 nm wavelenght, respectively. According to those analyses, this study suggests that bioactive compounds derived from BTM 11 were classified as the groups of flavonoids and feasible as potential candidates for anti-HCV therapy through the inhibitory effect of NS3 RNA helicase/ATPase activity. Keywords: Hepatitis C Virus, NS3 RNA helicase, ATPase, Microalga, Flavonoids 
Characterization of lipid productivity and fatty acid profile of three fast-growing microalgae isolated from Bengkulu for possible use in health application Praharyawan, Swastika; Rahman, Delicia Yunita; Susilaningsih, Dwi
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.03

Abstract

Three strains of fast-growing microalgae were investigated in this study for their potential of lipid production and its possible use in health application. Lipid content, lipid productivity, and fatty acids profile of the 3 mi-croalgae strains were also analyzed. The strain of LBB13-2-AL045 and LBB13-2-AL048 possessed highest lipid content (49.08 ± 0.25%) and lipid productivity (40.27 ± 1.91 mg.L-1.day-1), respectively, among the other tested strains. The fatty acids profile from the 3 strains exhibited its possible use in health application. The two strains of LBB13-2-AL046 and LBB13-2-AL048 possessed high valuable fatty acids of polyunsaturated fatty ac-ids (PUFA) omega-3 and omega-6, whereas LBB13-2- AL045 possessed balance ratio of saturated fatty acid (SFA) : monounsaturated fatty acid (MUFA) : PUFA (1 : 1.3 : 1) as is recommended by American Heart As-sociation (AHA) (1 : 1.4 : 0.8). The high content of omega-3 and omega-6 fatty acids indicated that the algal lipids of two strains (LBB13-2-AL046 and LBB13-2-AL048) were potential to be applied in cardiovascular health. The balance ratio of SFA : MUFA: PUFA as is recommended by AHA indicated that the algal oil of LBB13-2-AL045 strain was recommended in order to generate the best LDL/HDL ratio.
Produksi Biohidrogen dari Limbah Organik Cair Molase dan Vinasse Menggunakan Bakteri Rhodobium marinum Anhari, Saeful; Bintari, Siti Harnina; Mubarok, Ibnul; Susilaningsih, Dwi
Life Science Vol 5 No 2 (2016): October 2016
Publisher : Universitas Negeri Semarang

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Abstract

Biohidrogen merupakan salah satu sumber energi alternatif terbarukan yang dihasilkan melalui proses biologis menggunakan bahan baku biomassa organik dengan melibatkan mikroorganisme penghasil gas hidrogen. Penelitian ini bertujuan menentukan dan membandingkan rasio kebaharuan terhadap produksi gas biohidrogen dari limbah organik cair molase dan vinasse selama fotofermentasi menggunakan Rhodobium marinum. Penelitian ini menggunakan Rancangan Acak Lengkap tiga faktor, terdiri dari jenis limbah (molase dan vinasse), konsentrasi limbah (10%, 50%, 100%) dan pH limbah (6, 7, 8), pola perlakuan 2x3x3 dengan tiga kali ulangan. Penelitian ini memiliki alur penelitian: pembuatan media tumbuh bakteri, kultivasi bakteri, persiapan media produksi, karakterisasi media produksi, fotofermentasi, dan pengukuran kadar biohidrogen. Rerata hasil produksi gas biohidrogen limbah molase tersignifikan berturut-turut pada K1P3, K2P3, K3P3 sebesar 86/10-1 L kultur, 146/10-1 l kultur, 188/10-1 L kultur dan produksi gas biohidrogen limbah vinasse berturut-turut pada K2P3, K3P3 sebesar 86/ 10-1 L kultur, 110/ 10-1 L kultur. Kesimpulan yang diperoleh bahwa rasio kebaharuan produksi gas biohidrogen molase:vinasse sebesar 27:20. Produksi gas biohidrogen tertinggi pada limbah molase sebesar 188/10-1 L kultur dan limbah vinasse sebesar 110/10-1 L kultur. Biohydrogen is one alternative renewable energy sources produced through biological processes using organic biomass feedstocks involve hydrogen gas-producing microorganisms. This study aims to determine and compare the recency ratio biohydrogen to gas production from organic waste liquid molasses and vinasse by Rhodobium marinum fotofermentation. This study used a completely randomized design of three factors, comprised of the types of waste (molasses and vinasse), effluent concentration (10%, 50%, 100%) and a pH of waste (6, 7, 8) with a pattern of treatment 2x3x3 in three replications. The stages of research were: creation of bacterial growth media, bacteria cultivation, preparation of media production, media characterization of production, fotofermentation, and measurement of biohydrogen. Average results of biohydrogen gas production waste molasses for K1P3, K2P3, K3P3 as amount of 86 / 10-1 L culture, 146 / 10-1 L culture, 188 / 10-1 L culture, respectively and production biohidrogen gas of vinasse waste of K2P3, K3P3 by 86 / 10-1 L culture, 110 / 10-1 L culture, respectively. The conclusion that the newness ratio of biohydrogen production molasses: vinasse was at 27:20. The highest gas production biohydrogen on molasses waste by 188 / 10-1 l culture and vinasse waste by 110 / 10-1 L culture.
Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production Susilaningsih, Dwi; Harwati, Theresia Umi; Anam, Khairul; Yopi, Yopi
Makara Journal of Technology Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production. Addressing to the global warming problem, energy crisis and pollution, hydrogen production by micro-organisms using biotechnological approach should be considered, since it fulfils the recent society requirement to safely produce, renewable and environmental friendly energy. Hydrogen is one of the most promising green energy sources, because it is easily converted to electricity and cleanly combustible. There are three types of micro-organisms for hydrogen production, the first is cyanobacteria through the photosynthesis process, the second is anaerobic bacteria, which use organic substances as electron donor and energy and convert them to hydrogen, the third is photosynthetic bacteria, somewhat between photosynthetic and anaerobic bacteria, which are capable of converting the organic substances to hydrogen at a fairly high rate. We propose to use the abundant waste biomasses in Indonesia for hydrogen production by the microbial system. Our focus research is the production of hydrogen from waste biomasses by two-stage fermentation systems, which combine the conversion process of monomer biomasses to lactic acid by Lactobacillus sp. and the conversion process of lactic acid to hydrogen by photosynthetic bacteria. In this research, two kind substrates preparation were apply for woody waste biomass such as chemical hydrolysis and biological methods with several treatments. The results of the substrate preparation state showed that hydrolyses process of biomasses using strong acid are yielded total sugar about 70-90% of previous original content. Moreover, hydrolyses process using weak/diluted acid are yielded total sugar about 4-30% of original sugar. Furthermore, the biological treatments of degradation of woody waste biomasses are yielded total sugar about 0-10% (by single culture) and 10-50% (by consortium). Those hydrolysates substrates will use for fermentation two stages of lactate fermentation and conversion by photosynthetic bacteria in order to produce hydrogen gas.
Morphology and Molecular Characterization of Newly Isolated Microalgae Strain Chlorella volutis LIPI13-WKT066 from Wakatobi Islands and Its Potential Use Rahman, Delicia Yunita; Praharyawan, Swastika; Fadiyah, Farizul; Raharjo, Sapto; Susilaningsih, Dwi
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

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Abstract

Morphology and molecular characterization of microalgae isolated from Wakatobi Marine National Park was conducted. An understanding of the characteristics of morphology, molecular, as well as metabolites profile of the microalgae species is potentially useful for its future application. The primary aim of this study was to isolate, identify and characterize the microalgae strain isolated from Wakatobi Marine National Park labeled as LIPI13-WKT066 with the emphasis on the evaluation of amino acid content as a basis for its health application. Morphological observation under the microscope and molecular identification suggested that the microalgae strain of LIPI13-WKT066 belong to the strain under species of Chlorella volutis. Metabolite characterization of the microalgae strain showed that the content of protein (11.9%), lipid (12.4%) and carbohydrate (4.7%) was in the regular range. Further analysis of its amino acid content revealed the potency of the microalgae strain to be used as antihypertensive agent.
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
Publisher : BRIN

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Abstract

Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).
Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
Publisher : BRIN

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Abstract

BTM 11 is unknown species of microalgae which has active compounds that can inhibit viruses. Lectin is a carbohydrate-binding protein that is found in microalgae with antiviral and antibacterial activities. The purpose of this study was to perform identification, isolation, characterization, and assay of lectin inhibitory activity of microalgae BTM 11. The result shows that microalgae BTM 11 has homology with Cyanobacterium (99%) and Geitlerinema sp (98%). Lectin of microalgae BTM 11 has molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be an alternative to antiviral and antibacterial proteins.
Co-Authors A.A. Ketut Agung Cahyawan W Anam, M. Khairul Anhari, Saeful Antonius Budi Susanto Apon Zaenal Mustopa Delicia Yunita Rahma Fadiyah, Farizul Farida, Hilda Farida, Hilda Hadi Susilo, Hadi Hani Susanti, Hani Harayama, Shigeaki Harayama, Shigeaki Harwati, Theresia Umi Ibnul Mubarok Isworo, Rhestu Isworo, Rhestu Leenawaty Limantara Mala Nurilmala Ochieng, Fredrick Onyango Okazaki, Fumiyoshi Okazaki, Fumiyoshi Puspita Sari Harahap Putri, Prabawati Hyunita Putri, Prabawati Hyunita Rahman, Delicia Y. Rahman, Delicia Yunita Rifana Sobari Rifqiyah Nur Umami, Rifqiyah Nur Rosita Dwi Chandra, Rosita Dwi Salim, Katarina Purnomo Sapto Raharjo Siburian, Marlinang Diarta Siburian, Marlinang Diarta Siti Harnina Bintari Swastika Praharyawan, Swastika Swastika, I Nengah Syamsiar, Syamsiar Tatas H.P. Brotosudarmo Theresia Harwati, Theresia Topik Hidayat Tutik Murniasih Umoro, Asahedi UTUT WIDYASTUTI Widyaningrum, Dian Noverita Yantyati Widyastuti YOPI YOPI Yundari, Yundari