Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Veterinary Biomedical and Clinical Journal

The Analysis of Pancreatic Duodenal Homeobox 1 (PDX-1) Gene Mutation Based on Blood Glucose Level on Domestic Cats (Felis catus) Post Ovaryohisterectomy Putri, Hana Mitsuki; Aulani'am, Aulani'am; Nurmaningdyah, Ajeng Aeka; Prasetyo, Dodik; Permata, Fajar Shodiq
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.5

Abstract

Ovariohysterectomy (OH) in domestic cats causes several side effects, including obesity. Obesity is closely related to diabetes. The glucoregulatory gene that will cause insulin resistance if there is a gene mutation is the PDX-1 gene. This insulin resistance will have a very immediate effect on blood glucose in the body. The study examined the relationship between PDX-1 gene polymorphisms and blood glucose levels in domestic cats with OH. The method used in this study was in vitro PCR amplification of the PDX-1 gene with Forward primers 'CCG AGC TGT CAA AGC TAG CG' and Reverse 'GAA CCG GTA GAG GGT CTT GG' and followed by sequencing of PCR products. The results showed fundamental differences in the base sequence in the PDX-1 gene sequences at c.435G>A, c.927A>T, and c.584A>G. The fundamental difference in the PDX1 gene sequence is a silent mutation
Management of Avian Influenza H9N2 Vaccination in Broiler Chickens at JAPFA COMFEED Myanmar Pte Ltd. Verian, Bagas Ridjalu; Prasetyo, Dodik; Adrenalin, Sruti Listra; Wuragil, Dyah Kinasih; Cahyo, Budi Nur
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.3

Abstract

Avian influenza virus subtype H9N2 (AIV-H9N2) has been a concern for poultry health in the last 20 years. The virus emerged in domestic poultry in the mid-1990s and has been reported in several Asian countries, including Myanmar. AI subtype H9N2 caused huge economic losses to the poultry industry in the form of respiratory distress and 25% weight loss in broiler chickens. This study discusses the administration of Avian Influenza (AI) H9N2 strain inactivated vaccine to commercial broiler chickens at JAPFA COMFEED Myanmar Pte Ltd. The vaccination process was carried out through subcutaneous injection in the neck and intramuscular injection in the pectoralis musculus in the chest area of chickens at 11 days of age, with a dose of 0.5 ml per chicken. The vaccine used was VAKSIMUNE® AI H9 which uses oil as the emulsion material. Results from the HI serology test showed that 6 cages from Myothar and Wah Net Chaung farms showed protective antibody titers against Avian Influenza H9N2 virus at 30-40 days of age, with normal titer values or ≥24. This indicates that the AI subtype H9N2vaccine used is an effective antigen, capable of stimulating the broiler immune system, and has implications for increasing antibodies every week. This study contributes to the understanding of AI H9N2 vaccination management in commercial broilers at JAPFA COMFEED Myanmar Pte Ltd. With positive results in the serology test, it can be concluded that vaccination using VAKSIMUNE® AI H9 is effective in protecting broilers from Avian Influenza H9N2 infection, providing strong support to the sustainability of production and animal health in a commercial farm environment.