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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) JPBIO (Jurnal Pendidikan Biologi) YARSI Medical Journal Health and Medical Journal Jurnal MedScientiae Jurnal Bioteknologi & Biosains Indonesia (JBBI) Cermin Dunia Kedokteran Jurnal Bioteknologi & Biosains Indonesia
Kris Herawan Timotius
Krida Wacana Christian University, Jalan Arjuna Utara 6, Jakarta 11510, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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BIOTECHNOLOGY OF PRODIGIOSIN: RECENT DEVELOPMENTS AND TECHNOLOGICAL CHALLENGES Gunardi, Wani Devita; Margaretha; Timotius, Kris Herawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2010

Abstract

Background: Prodigiosin is produced by Serratia marcescens. It has several pharmacological benefits, such as anticancer, antimicrobial, and antidaibetic. However, prodigiosin production still faces problems because it cannot be produced effectively, efficiently, and cheaply. Objective: This study aimed to conduct a review that can explain the upstream and the downstream process in prodigiosin production. Methods: Articles were searched from PubMed and ScienceDirect with the keywords prodigiosin and Serratia marcescens from Juny until September 2023 including review and original article. Relevant data and information were then extracted. Results: Prodigiosin has spectrometrical characteristics, which are crucial for evaluating its production, extraction, and purification identification. Submerged or solid-state fermentation is applicable for prodigiosin production, but solid-state fermentation is better. The kind of growing substrates and the cultural condition influence it. The use of oil-based carbon sources is recommended for the high productivity of prodigiosin. In order to have a cheap, effective, and efficient production process, different experiments have been conducted. Standard extraction and purification methods can carry out the downstream process. Conclusion: Prodigiosin can be produced via submerged or solid-state fermentation. Using cheap and readily available substrate are the key to success for the upstream and downstream process. The standard extraction and purification methods are available.This findings can be used as a basis for further research regarding large-scale production of prodigiosin with the cheap, effective, and efficient methode.
MICROBIAL CONTAMINATION AND BIOACTIVE COMPOUNDS OF JAMU BERAS KENCUR Gunardi, Wani Devita; Teiseran, Virginia Marsella; Timotius, Kris Herawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5966

Abstract

Background: Jamu Beras Kencur (JBK) is recognized as an herbal medicine, containing two main components: the rhizome of Kaempferia galanga and rice. While previous research has identified bioactive compounds in galangal rhizomes, such as Ethyl p-Methoxycinnamate (EPMC), Ethyl-cinnamate, and Kaempferol, there are few reports on polar or aqueous compounds in JBK. LC-MS/MS and GC-MS enable comprehensive analysis of bioactive compounds, with LC-MS/MS detecting non-volatile, polar, and thermally sensitive compounds like flavonoids and glycosides, while GC-MS analyzes volatile and semi-volatile compounds, such as terpenoids, providing precise separation and identification. Therefore, this study were to know the amount and the growth of contaminant bacteria, yeast and mold; to determine the main bioactive compounds in JBK; and to determine the bioactive compound in aqueous and ethanolic extracts of rhizome that analysed with LC-MS/MS and GC-MS. Methode: JBK samples were sourced from local producers in West Jakarta, freshly prepared, and immediately analyzed for microbial contamination and bioactive compounds. Result: The analysis revealed microbial contamination in JBK, including Escherichia coli, Staphylococcus aureus, Coliform, yeast, and mold. Additionally, three novel flavonoid glycosides were identified: Chrysoeriol-4'-O-β-D-glucopyranoside, Patuletin-7-O-[6′′-(2-methylbutyryl)]-glucoside, and Acacetin-7-galactoside. Conclusion: Therefore, from the pharmacological perspective, JBK has the potentials as a healthy herbal drink. However, further preclinical and clinical studies are essential to validate its safety and efficacy for clinical use, which could pave the way for its integration into mainstream healthcare as a natural therapeutic option.
Co-Authors Ahmad Hamim Sadewa Donna Mesina Rosadini Pasaribu Flora Rumiati Hemi Sinorita Ignatio R. Haryono Ika Rahayu Kartika, Ronald Kartika, Ronald Winardi Kelvin Kelvin Margaretha Puspasari, Monica Sancnia, Sancnia Santoso, Adit Widodo Sartika, Chyntia Retna Simamora, Adelina Steven, Michael Sudrajat, Susana Elya Teiseran, Virginia Marsella Tena Djuartina, Tena Veronika Maria Sidharta Wani Devita Gunardi